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    <TITLE>Illumina MiSeq paired end sequencing; tilapia_ddrad_1</TITLE>
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        </POOL>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>tilapia_ddrad_1</LIBRARY_NAME>
        <LIBRARY_STRATEGY>OTHER</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>GENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>Restriction Digest</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED NOMINAL_LENGTH="455" NOMINAL_SDEV="135"/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Double digest RAD sequencing protocol was used with SbfI and SphI as restriction enzymes</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
      <SPOT_DESCRIPTOR>
        <SPOT_DECODE_SPEC>
          <SPOT_LENGTH>324</SPOT_LENGTH>
          <READ_SPEC>
            <READ_INDEX>0</READ_INDEX>
            <READ_LABEL>F</READ_LABEL>
            <READ_CLASS>Application Read</READ_CLASS>
            <READ_TYPE>Forward</READ_TYPE>
            <BASE_COORD>1</BASE_COORD>
          </READ_SPEC>
          <READ_SPEC>
            <READ_INDEX>1</READ_INDEX>
            <READ_LABEL>barcode_tag_a</READ_LABEL>
            <READ_CLASS>Technical Read</READ_CLASS>
            <READ_TYPE>BarCode</READ_TYPE>
            <EXPECTED_BASECALL_TABLE base_coord="1">
              <BASECALL read_group_tag="barcode1_TCAGA" min_match="5" max_mismatch="0" match_edge="full">TCAGA</BASECALL>
              <BASECALL read_group_tag="barcode1_GATCG" min_match="5" max_mismatch="0" match_edge="full">GATCG</BASECALL>
              <BASECALL read_group_tag="barcode1_CATGA" min_match="5" max_mismatch="0" match_edge="full">CATGA</BASECALL>
              <BASECALL read_group_tag="barcode1_ATCGA" min_match="5" max_mismatch="0" match_edge="full">ATCGA</BASECALL>
              <BASECALL read_group_tag="barcode1_TCGAG" min_match="5" max_mismatch="0" match_edge="full">TCGAG</BASECALL>
              <BASECALL read_group_tag="barcode1_GTCAC" min_match="5" max_mismatch="0" match_edge="full">GTCAC</BASECALL>
              <BASECALL read_group_tag="barcode1_GCATT" min_match="5" max_mismatch="0" match_edge="full">GCATT</BASECALL>
              <BASECALL read_group_tag="barcode1_CGATA" min_match="5" max_mismatch="0" match_edge="full">CGATA</BASECALL>
              <BASECALL read_group_tag="barcode1_TGCAACA" min_match="7" max_mismatch="0" match_edge="full">TGCAACA</BASECALL>
              <BASECALL read_group_tag="barcode1_CGTATCA" min_match="7" max_mismatch="0" match_edge="full">CGTATCA</BASECALL>
              <BASECALL read_group_tag="barcode1_CACAGAC" min_match="7" max_mismatch="0" match_edge="full">CACAGAC</BASECALL>
              <BASECALL read_group_tag="barcode1_ACTGCAC" min_match="7" max_mismatch="0" match_edge="full">ACTGCAC</BASECALL>
              <BASECALL read_group_tag="barcode1_TCTCTCA" min_match="7" max_mismatch="0" match_edge="full">TCTCTCA</BASECALL>
              <BASECALL read_group_tag="barcode1_GTACACA" min_match="7" max_mismatch="0" match_edge="full">GTACACA</BASECALL>
              <BASECALL read_group_tag="barcode1_CTCTTCA" min_match="7" max_mismatch="0" match_edge="full">CTCTTCA</BASECALL>
              <BASECALL read_group_tag="barcode1_CTAGGAC" min_match="7" max_mismatch="0" match_edge="full">CTAGGAC</BASECALL>
              <BASECALL read_group_tag="barcode1_ACGTA" min_match="5" max_mismatch="0" match_edge="full">ACGTA</BASECALL>
              <BASECALL read_group_tag="barcode1_AGAGT" min_match="5" max_mismatch="0" match_edge="full">AGAGT</BASECALL>
              <BASECALL read_group_tag="barcode1_ATGCT" min_match="5" max_mismatch="0" match_edge="full">ATGCT</BASECALL>
              <BASECALL read_group_tag="barcode1_GACTA" min_match="5" max_mismatch="0" match_edge="full">GACTA</BASECALL>
              <BASECALL read_group_tag="barcode1_CAGTCAC" min_match="7" max_mismatch="0" match_edge="full">CAGTCAC</BASECALL>
              <BASECALL read_group_tag="barcode1_GCTAACA" min_match="7" max_mismatch="0" match_edge="full">GCTAACA</BASECALL>
              <BASECALL read_group_tag="barcode1_ACACGAG" min_match="7" max_mismatch="0" match_edge="full">ACACGAG</BASECALL>
              <BASECALL read_group_tag="barcode1_AGGACAC" min_match="7" max_mismatch="0" match_edge="full">AGGACAC</BASECALL>
            </EXPECTED_BASECALL_TABLE>
          </READ_SPEC>
          <READ_SPEC>
            <READ_INDEX>2</READ_INDEX>
            <READ_LABEL>R</READ_LABEL>
            <READ_CLASS>Application Read</READ_CLASS>
            <READ_TYPE>Reverse</READ_TYPE>
            <BASE_COORD>163</BASE_COORD>
          </READ_SPEC>
          <READ_SPEC>
            <READ_INDEX>3</READ_INDEX>
            <READ_LABEL>barcode_tag_b</READ_LABEL>
            <READ_CLASS>Technical Read</READ_CLASS>
            <READ_TYPE>BarCode</READ_TYPE>
            <EXPECTED_BASECALL_TABLE base_coord="163">
              <BASECALL read_group_tag="barcode2_CGATC" min_match="5" max_mismatch="0" match_edge="full">CGATC</BASECALL>
              <BASECALL read_group_tag="barcode2_CATCTGT" min_match="7" max_mismatch="0" match_edge="full">CATCTGT</BASECALL>
              <BASECALL read_group_tag="barcode2_CTGGT" min_match="5" max_mismatch="0" match_edge="full">CTGGT</BASECALL>
              <BASECALL read_group_tag="barcode2_GTCAAGT" min_match="7" max_mismatch="0" match_edge="full">GTCAAGT</BASECALL>
              <BASECALL read_group_tag="barcode2_GAAGC" min_match="5" max_mismatch="0" match_edge="full">GAAGC</BASECALL>
              <BASECALL read_group_tag="barcode2_ATACGGT" min_match="7" max_mismatch="0" match_edge="full">ATACGGT</BASECALL>
            </EXPECTED_BASECALL_TABLE>
          </READ_SPEC>
        </SPOT_DECODE_SPEC>
      </SPOT_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina MiSeq</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
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</EXPERIMENT_SET>
