ERX538424 E-MTAB-2816:E8.5 Sox17 assay 2 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527817 SAMEA2700083 E-MTAB-2816:E8.5 Sox17^GFP/+ embryos 2 E8.5 Sox17^GFP/+ RNA 2 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 110 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Sox17-expressing definitive endodermal cell Experimental Factor: developmental stage embryonic day 8.5 ERX538425 E-MTAB-2816:E9.5 Pdx1^CFP/+ assay 3 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527818 SAMEA2700084 E-MTAB-2816:E9.5 Pdx1^CFP/+ embryos 3 E9.5 Pdx1^CFP/+ RNA 3 RNA-Seq TRANSCRIPTOMIC cDNA See file RNA_extraction_protocol_for_FACS-isolated_cells.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 101 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Pdx1-expressing foregut endodermal cell Experimental Factor: developmental stage embryonic day 9.5 ERX538426 E-MTAB-2816:E9.5 Pdx1^CFP/+ assay 2 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527819 SAMEA2700085 E-MTAB-2816:E9.5 Pdx1^CFP/+ embryos 2 E9.5 Pdx1^CFP/+ RNA 2 RNA-Seq TRANSCRIPTOMIC cDNA See file RNA_extraction_protocol_for_FACS-isolated_cells.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 101 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Pdx1-expressing foregut endodermal cell Experimental Factor: developmental stage embryonic day 9.5 ERX538427 E-MTAB-2816:E15.5 Ngn3^GFP/+ assay 1 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527820 SAMEA2700086 E-MTAB-2816:E15.5 Ngn3^GFP/+ embryos 1 E15.5 Ngn3^GFP/+ RNA 1 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type neurogenin-3-expressing endocrine progenitor cell Experimental Factor: developmental stage embryonic day 15.5 ERX538428 E-MTAB-2816:E15.5 Insm1^GFP/+ assay 1 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527821 SAMEA2700087 E-MTAB-2816:E15.5 Insm1^GFP/+ embryos 1 E15.5 Insm1^GFP/+ RNA 1 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Insm1-expressing endocrine progenitor cell Experimental Factor: developmental stage embryonic day 15.5 ERX538429 E-MTAB-2816:E8.0 Sox17 assay 3 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527822 SAMEA2700088 E-MTAB-2816:E8.0 Sox17^GFP/+ embryos 3 E8.0 Sox17^GFP/+ RNA 3 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 110 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Sox17-expressing definitive endodermal cell Experimental Factor: developmental stage embryonic day 8 ERX538430 E-MTAB-2816:E16.5 MIP-GFP assay 1 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527823 SAMEA2700089 E-MTAB-2816:E16.5 MIP-GFP embryos 1 E16.5 MIP-GFP RNA 1 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 105 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type insulin-expressing immature beta cell Experimental Factor: developmental stage embryonic day 16.5 ERX538431 E-MTAB-2816:E8.0 Sox17 assay 1 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527824 SAMEA2700090 E-MTAB-2816:E8.0 Sox17^GFP/+ embryos 1 E8.0 Sox17^GFP/+ RNA 1 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 110 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Sox17-expressing definitive endodermal cell Experimental Factor: developmental stage embryonic day 8 ERX538432 E-MTAB-2816:E15.5 Insm1^GFP/GFP assay 2 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527825 SAMEA2700091 E-MTAB-2816:E15.5 Insm1^GFP/GFP embryos 2 E15.5 Insm1^GFP/GFP RNA 2 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Insm1-lacking endocrine progenitor cell Experimental Factor: developmental stage embryonic day 15.5 ERX538433 E-MTAB-2816:E8.0 Sox17 assay 2 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527826 SAMEA2700092 E-MTAB-2816:E8.0 Sox17^GFP/+ embryos 2 E8.0 Sox17^GFP/+ RNA 2 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 110 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Sox17-expressing definitive endodermal cell Experimental Factor: developmental stage embryonic day 8 ERX538434 E-MTAB-2816:E9.5 Pdx1^CFP/+ assay 1 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527827 SAMEA2700093 E-MTAB-2816:E9.5 Pdx1^CFP/+ embryos 1 E9.5 Pdx1^CFP/+ RNA 1 RNA-Seq TRANSCRIPTOMIC cDNA See file RNA_extraction_protocol_for_FACS-isolated_cells.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 101 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Pdx1-expressing foregut endodermal cell Experimental Factor: developmental stage embryonic day 9.5 ERX538435 E-MTAB-2816:E15.5 Ngn3^GFP/+ assay 3 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527828 SAMEA2700094 E-MTAB-2816:E15.5 Ngn3^GFP/+ embryos 3 E15.5 Ngn3^GFP/+ RNA 3 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type neurogenin-3-expressing endocrine progenitor cell Experimental Factor: developmental stage embryonic day 15.5 ERX538436 E-MTAB-2816:E9.5 Sox17 EpCAM+ assay 2 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527829 SAMEA2700095 E-MTAB-2816:E9.5 Sox17^GFP/+ embryos 2 E8.5 Sox17^GFP/+ EpCAM+ RNA 2 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 110 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Sox17-expressing Epcam+ pancreatic epithelial cell Experimental Factor: developmental stage embryonic day 9.5 ERX538437 E-MTAB-2816:E15.5 Insm1^GFP/GFP assay 3 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527830 SAMEA2700096 E-MTAB-2816:E15.5 Insm1^GFP/GFP embryos 3 E15.5 Insm1^GFP/GFP RNA 3 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Insm1-lacking endocrine progenitor cell Experimental Factor: developmental stage embryonic day 15.5 ERX538438 E-MTAB-2816:E10.5 Ptf1a^YFP/+ assay 1 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527831 SAMEA2700097 E-MTAB-2816:E10.5 Ptf1a^YFP/+ embryos 1 E10.5 Ptf1a^YFP/+ RNA 1 RNA-Seq TRANSCRIPTOMIC cDNA See file RNA_extraction_protocol_for_FACS-isolated_cells.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Ptf1a-expressing multipotent pancreatic cell Experimental Factor: developmental stage embryonic day 10.5 ERX538439 E-MTAB-2816:E15.5 Insm1^GFP/+ assay 3 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527832 SAMEA2700098 E-MTAB-2816:E15.5 Insm1^GFP/+ embryos 3 E15.5 Insm1^GFP/+ RNA 3 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Insm1-expressing endocrine progenitor cell Experimental Factor: developmental stage embryonic day 15.5 ERX538440 E-MTAB-2816:E8.5 Sox17 assay 3 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527833 SAMEA2700099 E-MTAB-2816:E8.5 Sox17^GFP/+ embryos 3 E8.5 Sox17^GFP/+ RNA 3 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 110 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Sox17-expressing definitive endodermal cell Experimental Factor: developmental stage embryonic day 8.5 ERX538441 E-MTAB-2816:E15.5 Ngn3^GFP/GFP assay 1 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527834 SAMEA2700100 E-MTAB-2816:E15.5 Ngn3^GFP/GFP embryos 1 E15.5 Ngn3^GFP/GFP RNA 1 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type neurogenin-3-lacking pancreatic progenitor cell Experimental Factor: developmental stage embryonic day 15.5 ERX538442 E-MTAB-2816:E15.5 Ngn3^GFP/+ assay 2 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527835 SAMEA2700101 E-MTAB-2816:E15.5 Ngn3^GFP/+ embryos 2 E15.5 Ngn3^GFP/+ RNA 2 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type neurogenin-3-expressing endocrine progenitor cell Experimental Factor: developmental stage embryonic day 15.5 ERX538443 E-MTAB-2816:E8.5 Sox17 assay 1 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527836 SAMEA2700102 E-MTAB-2816:E8.5 Sox17^GFP/+ embryos 1 E8.5 Sox17^GFP/+ RNA 1 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 110 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Sox17-expressing definitive endodermal cell Experimental Factor: developmental stage embryonic day 8.5 ERX538444 E-MTAB-2816:P60 MIP-GFP assay 3 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527837 SAMEA2700103 E-MTAB-2816:P60 MIP-GFP mice 3 P60 MIP-GFP RNA 3 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type insulin-expressing mature beta cell Experimental Factor: developmental stage postnatal day 60 ERX538445 E-MTAB-2816:E16.5 MIP-GFP assay 3 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527838 SAMEA2700104 E-MTAB-2816:E16.5 MIP-GFP embryos 3 E16.5 MIP-GFP RNA 3 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 107 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type insulin-expressing immature beta cell Experimental Factor: developmental stage embryonic day 16.5 ERX538446 E-MTAB-2816:E10.5 Ptf1a^YFP/+ assay 2 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527839 SAMEA2700105 E-MTAB-2816:E10.5 Ptf1a^YFP/+ embryos 2 E10.5 Ptf1a^YFP/+ RNA 2 RNA-Seq TRANSCRIPTOMIC cDNA See file RNA_extraction_protocol_for_FACS-isolated_cells.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Ptf1a-expressing multipotent pancreatic cell Experimental Factor: developmental stage embryonic day 10.5 ERX538447 E-MTAB-2816:E15.5 Insm1^GFP/GFP assay 1 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527840 SAMEA2700106 E-MTAB-2816:E15.5 Insm1^GFP/GFP embryos 1 E15.5 Insm1^GFP/GFP RNA 1 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Insm1-lacking endocrine progenitor cell Experimental Factor: developmental stage embryonic day 15.5 ERX538448 E-MTAB-2816:E15.5 Ngn3^GFP/GFP assay 3 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527841 SAMEA2700107 E-MTAB-2816:E15.5 Ngn3^GFP/GFP embryos 3 E15.5 Ngn3^GFP/GFP RNA 3 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type neurogenin-3-lacking pancreatic progenitor cell Experimental Factor: developmental stage embryonic day 15.5 ERX538449 E-MTAB-2816:E15.5 Ngn3^GFP/GFP assay 2 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527842 SAMEA2700108 E-MTAB-2816:E15.5 Ngn3^GFP/GFP embryos 2 E15.5 Ngn3^GFP/GFP RNA 2 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type neurogenin-3-lacking pancreatic progenitor cell Experimental Factor: developmental stage embryonic day 15.5 ERX538450 E-MTAB-2816:P60 MIP-GFP assay 2 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527843 SAMEA2700109 E-MTAB-2816:P60 MIP-GFP mice 2 P60 MIP-GFP RNA 2 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type insulin-expressing mature beta cell Experimental Factor: developmental stage postnatal day 60 ERX538451 E-MTAB-2816:E9.5 Sox17 EpCAM+ assay 1 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527844 SAMEA2700110 E-MTAB-2816:E9.5 Sox17^GFP/+ embryos 1 E8.5 Sox17^GFP/+ EpCAM+ RNA 1 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 110 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Sox17-expressing Epcam+ pancreatic epithelial cell Experimental Factor: developmental stage embryonic day 9.5 ERX538452 E-MTAB-2816:E16.5 MIP-GFP assay 2 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527845 SAMEA2700111 E-MTAB-2816:E16.5 MIP-GFP embryos 2 E16.5 MIP-GFP RNA 2 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 106 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type insulin-expressing immature beta cell Experimental Factor: developmental stage embryonic day 16.5 ERX538453 E-MTAB-2816:E10.5 Ptf1a^YFP/+ assay 3 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527846 SAMEA2700112 E-MTAB-2816:E10.5 Ptf1a^YFP/+ embryos 3 E10.5 Ptf1a^YFP/+ RNA 3 RNA-Seq TRANSCRIPTOMIC cDNA See file RNA_extraction_protocol_for_FACS-isolated_cells.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Ptf1a-expressing multipotent pancreatic cell Experimental Factor: developmental stage embryonic day 10.5 ERX538454 E-MTAB-2816:P60 MIP-GFP assay 1 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527847 SAMEA2700113 E-MTAB-2816:P60 MIP-GFP mice 1 P60 MIP-GFP RNA 1 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type insulin-expressing mature beta cell Experimental Factor: developmental stage postnatal day 60 ERX538455 E-MTAB-2816:E15.5 Insm1^GFP/+ assay 2 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527848 SAMEA2700114 E-MTAB-2816:E15.5 Insm1^GFP/+ embryos 2 E15.5 Insm1^GFP/+ RNA 2 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 111 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Insm1-expressing endocrine progenitor cell Experimental Factor: developmental stage embryonic day 15.5 ERX538456 E-MTAB-2816:E9.5 Sox17 EpCAM+ assay 3 ERP006673 E-MTAB-2816 Whole transcriptome profiling of beta cell development in the mouse ERS527849 SAMEA2700115 E-MTAB-2816:E9.5 Sox17^GFP/+ embryos 3 E8.5 Sox17^GFP/+ EpCAM+ RNA 3 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008). 110 0 Application Read Forward 1 Illumina HiSeq 2000 Experimental Factor: cell type Sox17-expressing Epcam+ pancreatic epithelial cell Experimental Factor: developmental stage embryonic day 9.5