ERS527817 SAMEA2700083 10090 Mus musculus pool of 9 E8.5 (8~9 somites) Sox17^GFP/+ embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The embryos were dissected and the dissection event was confirmed by checking the marker protein (e.g., GFP) fluorescence. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:35Z External Id SAMEA2700083 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:35Z INSDC status public Submitter Id E-MTAB-2816:E8.5 Sox17^GFP/+ embryos 2 age measurement datum embryonic day 8.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E8.5 Sox17^GFP/+ embryos 2 scientific_name Mus musculus strain STOCK Sox17tm2(EGFP/cre)Mgn/Mmnc mouse strain ERS527818 SAMEA2700084 10090 Mus musculus pool of 6 E9.5 Pdx1^CFP/+ embryos (2 litters) Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The embryos were dissected and the dissection event was confirmed by checking the marker protein (e.g., GFP) fluorescence. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:35Z External Id SAMEA2700084 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:35Z INSDC status public Submitter Id E-MTAB-2816:E9.5 Pdx1^CFP/+ embryos 3 age measurement datum embryonic day 9.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E9.5 Pdx1^CFP/+ embryos 3 scientific_name Mus musculus strain B6.129S6-Pdx1tm2Mgn/Mmnc mouse strain ERS527819 SAMEA2700085 10090 Mus musculus pool 4 E9.5 Pdx1^CFP/+ embryos (2 litters) Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The embryos were dissected and the dissection event was confirmed by checking the marker protein (e.g., GFP) fluorescence. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:35Z External Id SAMEA2700085 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:35Z INSDC status public Submitter Id E-MTAB-2816:E9.5 Pdx1^CFP/+ embryos 2 age measurement datum embryonic day 9.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E9.5 Pdx1^CFP/+ embryos 2 scientific_name Mus musculus strain B6.129S6-Pdx1tm2Mgn/Mmnc mouse strain ERS527820 SAMEA2700086 10090 Mus musculus pool of 7 E15.5 Ngn3^GFP/+ embryos (2 litters) Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:35Z External Id SAMEA2700086 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:35Z INSDC status public Submitter Id E-MTAB-2816:E15.5 Ngn3^GFP/+ embryos 1 age measurement datum embryonic day 15.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E15.5 Ngn3^GFP/+ embryos 1 scientific_name Mus musculus strain B6.129S-Neurog3tm1(EGFP)Khk/Mmcd mouse strain ERS527821 SAMEA2700087 10090 Mus musculus pool of 2 E15.5 Insm1^GFP/+ embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Heterozygous embryos were identified using a fluorescent microscope, and embryos obtained from heterozygous interbreeding were genotyped using custom-designed TaqMan primers and probes. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:35Z External Id SAMEA2700087 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:35Z INSDC status public Submitter Id E-MTAB-2816:E15.5 Insm1^GFP/+ embryos 1 age measurement datum embryonic day 15.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E15.5 Insm1^GFP/+ embryos 1 scientific_name Mus musculus strain Insm1tm2Mgn mouse strain ERS527822 SAMEA2700088 10090 Mus musculus pool of 13 E8.0 (5~7 somites) Sox17^GFP/+ embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The embryos were dissected and the dissection event was confirmed by checking the marker protein (e.g., GFP) fluorescence. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:35Z External Id SAMEA2700088 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:35Z INSDC status public Submitter Id E-MTAB-2816:E8.0 Sox17^GFP/+ embryos 3 age measurement datum embryonic day 8 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E8.0 Sox17^GFP/+ embryos 3 scientific_name Mus musculus strain STOCK Sox17tm2(EGFP/cre)Mgn/Mmnc mouse strain ERS527823 SAMEA2700089 10090 Mus musculus pool of 4 E16.5 MIP-GFP embryos Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:35Z External Id SAMEA2700089 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:35Z INSDC status public Submitter Id E-MTAB-2816:E16.5 MIP-GFP embryos 1 age measurement datum embryonic day 16.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E16.5 MIP-GFP embryos 1 scientific_name Mus musculus strain STOCK Tg(Ins1-EGFP/GH1)14Hara/Mmmh mouse strain ERS527824 SAMEA2700090 10090 Mus musculus pool of 6 E8.0 (5~7 somites) Sox17^GFP/+ embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The embryos were dissected and the dissection event was confirmed by checking the marker protein (e.g., GFP) fluorescence. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:35Z External Id SAMEA2700090 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:35Z INSDC status public Submitter Id E-MTAB-2816:E8.0 Sox17^GFP/+ embryos 1 age measurement datum embryonic day 8 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E8.0 Sox17^GFP/+ embryos 1 scientific_name Mus musculus strain STOCK Sox17tm2(EGFP/cre)Mgn/Mmnc mouse strain ERS527825 SAMEA2700091 10090 Mus musculus pool 1 of 5 E15.5 Insm1^GFP/GFP embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Heterozygous embryos were identified using a fluorescent microscope, and embryos obtained from heterozygous interbreeding were genotyped using custom-designed TaqMan primers and probes. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:58Z External Id SAMEA2700091 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:58Z INSDC status public Submitter Id E-MTAB-2816:E15.5 Insm1^GFP/GFP embryos 2 age measurement datum embryonic day 15.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E15.5 Insm1^GFP/GFP embryos 2 scientific_name Mus musculus strain Insm1tm2Mgn mouse strain ERS527826 SAMEA2700092 10090 Mus musculus pool of 11 E8.0 (6~7 somites) Sox17^GFP/+ embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The embryos were dissected and the dissection event was confirmed by checking the marker protein (e.g., GFP) fluorescence. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:58Z External Id SAMEA2700092 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:58Z INSDC status public Submitter Id E-MTAB-2816:E8.0 Sox17^GFP/+ embryos 2 age measurement datum embryonic day 8 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E8.0 Sox17^GFP/+ embryos 2 scientific_name Mus musculus strain STOCK Sox17tm2(EGFP/cre)Mgn/Mmnc mouse strain ERS527827 SAMEA2700093 10090 Mus musculus pool of 5 E9.5 Pdx1^CFP/+ embryos (2 litters) Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The embryos were dissected and the dissection event was confirmed by checking the marker protein (e.g., GFP) fluorescence. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:58Z External Id SAMEA2700093 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:58Z INSDC status public Submitter Id E-MTAB-2816:E9.5 Pdx1^CFP/+ embryos 1 age measurement datum embryonic day 9.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E9.5 Pdx1^CFP/+ embryos 1 scientific_name Mus musculus strain B6.129S6-Pdx1tm2Mgn/Mmnc mouse strain ERS527828 SAMEA2700094 10090 Mus musculus pool of 23 E15.5 Ngn3^GFP/+ embryos (8 litters) Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:58Z External Id SAMEA2700094 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:58Z INSDC status public Submitter Id E-MTAB-2816:E15.5 Ngn3^GFP/+ embryos 3 age measurement datum embryonic day 15.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E15.5 Ngn3^GFP/+ embryos 3 scientific_name Mus musculus strain B6.129S-Neurog3tm1(EGFP)Khk/Mmcd mouse strain ERS527829 SAMEA2700095 10090 Mus musculus pool of 27 E9.5 Sox17^GFP/+ embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The embryos were dissected and the dissection event was confirmed by checking the marker protein (e.g., GFP) fluorescence. Cells were immuno-labeled. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:58Z External Id SAMEA2700095 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:58Z INSDC status public Submitter Id E-MTAB-2816:E9.5 Sox17^GFP/+ embryos 2 age measurement datum embryonic day 9.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E9.5 Sox17^GFP/+ embryos 2 scientific_name Mus musculus strain STOCK Sox17tm2(EGFP/cre)Mgn/Mmnc mouse strain ERS527830 SAMEA2700096 10090 Mus musculus pool 2 of 5 E15.5 Insm1^GFP/GFP embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Heterozygous embryos were identified using a fluorescent microscope, and embryos obtained from heterozygous interbreeding were genotyped using custom-designed TaqMan primers and probes. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:58Z External Id SAMEA2700096 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:58Z INSDC status public Submitter Id E-MTAB-2816:E15.5 Insm1^GFP/GFP embryos 3 age measurement datum embryonic day 15.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E15.5 Insm1^GFP/GFP embryos 3 scientific_name Mus musculus strain Insm1tm2Mgn mouse strain ERS527831 SAMEA2700097 10090 Mus musculus pool of 11 E10.5 Ptf1a^YFP/+ embryos (2 litters) Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Heterozygous embryos were identified using a fluorescent microscope, and embryos obtained from heterozygous interbreeding were genotyped using custom-designed TaqMan primers and probes. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:58Z External Id SAMEA2700097 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:58Z INSDC status public Submitter Id E-MTAB-2816:E10.5 Ptf1a^YFP/+ embryos 1 age measurement datum embryonic day 10.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E10.5 Ptf1a^YFP/+ embryos 1 scientific_name Mus musculus strain STOCK Ptf1atm1.1Mgn/Mmmh mouse strain ERS527832 SAMEA2700098 10090 Mus musculus pool of 5 E15.5 Insm1^GFP/+ embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Heterozygous embryos were identified using a fluorescent microscope, and embryos obtained from heterozygous interbreeding were genotyped using custom-designed TaqMan primers and probes. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:58Z External Id SAMEA2700098 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:58Z INSDC status public Submitter Id E-MTAB-2816:E15.5 Insm1^GFP/+ embryos 3 age measurement datum embryonic day 15.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E15.5 Insm1^GFP/+ embryos 3 scientific_name Mus musculus strain Insm1tm2Mgn mouse strain ERS527833 SAMEA2700099 10090 Mus musculus pool of 12 E8.5 (8~10 somites) Sox17^GFP/+ embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The embryos were dissected and the dissection event was confirmed by checking the marker protein (e.g., GFP) fluorescence. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:58Z External Id SAMEA2700099 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:58Z INSDC status public Submitter Id E-MTAB-2816:E8.5 Sox17^GFP/+ embryos 3 age measurement datum embryonic day 8.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E8.5 Sox17^GFP/+ embryos 3 scientific_name Mus musculus strain STOCK Sox17tm2(EGFP/cre)Mgn/Mmnc mouse strain ERS527834 SAMEA2700100 10090 Mus musculus pool of 6 E15.5 Ngn3^GFP/GFP embryos (4 litters) Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:59Z External Id SAMEA2700100 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:59Z INSDC status public Submitter Id E-MTAB-2816:E15.5 Ngn3^GFP/GFP embryos 1 age measurement datum embryonic day 15.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E15.5 Ngn3^GFP/GFP embryos 1 scientific_name Mus musculus strain B6.129S-Neurog3tm1(EGFP)Khk/Mmcd mouse strain ERS527835 SAMEA2700101 10090 Mus musculus pool of 16 E15.5 Ngn3^GFP/+ embryos (4 litters) Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:14:59Z External Id SAMEA2700101 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:14:59Z INSDC status public Submitter Id E-MTAB-2816:E15.5 Ngn3^GFP/+ embryos 2 age measurement datum embryonic day 15.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E15.5 Ngn3^GFP/+ embryos 2 scientific_name Mus musculus strain B6.129S-Neurog3tm1(EGFP)Khk/Mmcd mouse strain ERS527836 SAMEA2700102 10090 Mus musculus pool of 7 E8.5 (8~10 somites) Sox17^GFP/+ embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The embryos were dissected and the dissection event was confirmed by checking the marker protein (e.g., GFP) fluorescence. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:16:28Z External Id SAMEA2700102 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:16:28Z INSDC status public Submitter Id E-MTAB-2816:E8.5 Sox17^GFP/+ embryos 1 age measurement datum embryonic day 8.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E8.5 Sox17^GFP/+ embryos 1 scientific_name Mus musculus strain STOCK Sox17tm2(EGFP/cre)Mgn/Mmnc mouse strain ERS527837 SAMEA2700103 10090 Mus musculus 2 adult P60 MIP-GFP mice Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:16:28Z External Id SAMEA2700103 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:16:28Z INSDC status public Submitter Id E-MTAB-2816:P60 MIP-GFP mice 3 age measurement datum postnatal day 60 broker name ArrayExpress common name house mouse life cycle stage post-juvenile adult stage sample name E-MTAB-2816:P60 MIP-GFP mice 3 scientific_name Mus musculus strain STOCK Tg(Ins1-EGFP/GH1)14Hara/Mmmh mouse strain ERS527838 SAMEA2700104 10090 Mus musculus pool of 4 E16.5 MIP-GFP embryos Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:16:28Z External Id SAMEA2700104 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:16:28Z INSDC status public Submitter Id E-MTAB-2816:E16.5 MIP-GFP embryos 3 age measurement datum embryonic day 16.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E16.5 MIP-GFP embryos 3 scientific_name Mus musculus strain STOCK Tg(Ins1-EGFP/GH1)14Hara/Mmmh mouse strain ERS527839 SAMEA2700105 10090 Mus musculus pool of 21 E10.5 Ptf1a^YFP/+ embryos (3 litters) Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Heterozygous embryos were identified using a fluorescent microscope, and embryos obtained from heterozygous interbreeding were genotyped using custom-designed TaqMan primers and probes. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:16:28Z External Id SAMEA2700105 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:16:28Z INSDC status public Submitter Id E-MTAB-2816:E10.5 Ptf1a^YFP/+ embryos 2 age measurement datum embryonic day 10.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E10.5 Ptf1a^YFP/+ embryos 2 scientific_name Mus musculus strain STOCK Ptf1atm1.1Mgn/Mmmh mouse strain ERS527840 SAMEA2700106 10090 Mus musculus pool of 2 E15.5 Insm1^GFP/GFP embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Heterozygous embryos were identified using a fluorescent microscope, and embryos obtained from heterozygous interbreeding were genotyped using custom-designed TaqMan primers and probes. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:16:29Z External Id SAMEA2700106 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:16:29Z INSDC status public Submitter Id E-MTAB-2816:E15.5 Insm1^GFP/GFP embryos 1 age measurement datum embryonic day 15.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E15.5 Insm1^GFP/GFP embryos 1 scientific_name Mus musculus strain Insm1tm2Mgn mouse strain ERS527841 SAMEA2700107 10090 Mus musculus pool of 15 E15.5 Ngn3^GFP/GFP embryos (8 litters) Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:16:29Z External Id SAMEA2700107 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:16:29Z INSDC status public Submitter Id E-MTAB-2816:E15.5 Ngn3^GFP/GFP embryos 3 age measurement datum embryonic day 15.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E15.5 Ngn3^GFP/GFP embryos 3 scientific_name Mus musculus strain B6.129S-Neurog3tm1(EGFP)Khk/Mmcd mouse strain ERS527842 SAMEA2700108 10090 Mus musculus pool of 3 E15.5 Ngn3^GFP/GFP embryos (2 litters) Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:16:29Z External Id SAMEA2700108 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:16:29Z INSDC status public Submitter Id E-MTAB-2816:E15.5 Ngn3^GFP/GFP embryos 2 age measurement datum embryonic day 15.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E15.5 Ngn3^GFP/GFP embryos 2 scientific_name Mus musculus strain B6.129S-Neurog3tm1(EGFP)Khk/Mmcd mouse strain ERS527843 SAMEA2700109 10090 Mus musculus 2 adult P60 MIP-GFP mice Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:16:29Z External Id SAMEA2700109 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:16:29Z INSDC status public Submitter Id E-MTAB-2816:P60 MIP-GFP mice 2 age measurement datum postnatal day 60 broker name ArrayExpress common name house mouse life cycle stage post-juvenile adult stage sample name E-MTAB-2816:P60 MIP-GFP mice 2 scientific_name Mus musculus strain STOCK Tg(Ins1-EGFP/GH1)14Hara/Mmmh mouse strain ERS527844 SAMEA2700110 10090 Mus musculus pool of 29 E9.5 Sox17^GFP/+ embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The embryos were dissected and the dissection event was confirmed by checking the marker protein (e.g., GFP) fluorescence. Cells were immuno-labeled. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:16:29Z External Id SAMEA2700110 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:16:29Z INSDC status public Submitter Id E-MTAB-2816:E9.5 Sox17^GFP/+ embryos 1 age measurement datum embryonic day 9.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E9.5 Sox17^GFP/+ embryos 1 scientific_name Mus musculus strain STOCK Sox17tm2(EGFP/cre)Mgn/Mmnc mouse strain ERS527845 SAMEA2700111 10090 Mus musculus pool of 6 E16.5 MIP-GFP embryos Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:16:29Z External Id SAMEA2700111 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:16:29Z INSDC status public Submitter Id E-MTAB-2816:E16.5 MIP-GFP embryos 2 age measurement datum embryonic day 16.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E16.5 MIP-GFP embryos 2 scientific_name Mus musculus strain STOCK Tg(Ins1-EGFP/GH1)14Hara/Mmmh mouse strain ERS527846 SAMEA2700112 10090 Mus musculus pool of 32 E10.5 Ptf1a^YFP/+ embryos (6 litters) Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Heterozygous embryos were identified using a fluorescent microscope, and embryos obtained from heterozygous interbreeding were genotyped using custom-designed TaqMan primers and probes. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:16:29Z External Id SAMEA2700112 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:16:29Z INSDC status public Submitter Id E-MTAB-2816:E10.5 Ptf1a^YFP/+ embryos 3 age measurement datum embryonic day 10.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E10.5 Ptf1a^YFP/+ embryos 3 scientific_name Mus musculus strain STOCK Ptf1atm1.1Mgn/Mmmh mouse strain ERS527847 SAMEA2700113 10090 Mus musculus 2 adult P60 MIP-GFP mice Protocols: Embryos were isolated from mice; the presence of vaginal plug at noon was considered as E0.5. Embryos obtained from heterozygous interbreeding were genotyped by fluorescence microscopy or were genotyped using PCR. Pancreatic tissues were dissociated with trypsin at 37 C for 10-60 minutes. Adult pancreas were perfused, islets handpicked, and then subject to sorting. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. Littermates without EGFP were used as controls to set gating. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:18:18Z External Id SAMEA2700113 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:18:18Z INSDC status public Submitter Id E-MTAB-2816:P60 MIP-GFP mice 1 age measurement datum postnatal day 60 broker name ArrayExpress common name house mouse life cycle stage post-juvenile adult stage sample name E-MTAB-2816:P60 MIP-GFP mice 1 scientific_name Mus musculus strain STOCK Tg(Ins1-EGFP/GH1)14Hara/Mmmh mouse strain ERS527848 SAMEA2700114 10090 Mus musculus pool of 3 E15.5 Insm1^GFP/+ embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Heterozygous embryos were identified using a fluorescent microscope, and embryos obtained from heterozygous interbreeding were genotyped using custom-designed TaqMan primers and probes. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:18:18Z External Id SAMEA2700114 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:18:18Z INSDC status public Submitter Id E-MTAB-2816:E15.5 Insm1^GFP/+ embryos 2 age measurement datum embryonic day 15.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E15.5 Insm1^GFP/+ embryos 2 scientific_name Mus musculus strain Insm1tm2Mgn mouse strain ERS527849 SAMEA2700115 10090 Mus musculus pool of 26 E9.5 Sox17^GFP/+ embryos Protocols: Embryos were isolated from mice on specified day of development; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The embryos were dissected and the dissection event was confirmed by checking the marker protein (e.g., GFP) fluorescence. Cells were immuno-labeled. Tissues were dissociated with Accumax (Sigma) at 37 C. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II. ENA-FIRST-PUBLIC 2021-02-19T04:08:52Z ENA-LAST-UPDATE 2018-03-08T19:18:18Z External Id SAMEA2700115 INSDC center name Vanderbilt University INSDC first public 2021-02-19T04:08:52Z INSDC last update 2018-03-08T19:18:18Z INSDC status public Submitter Id E-MTAB-2816:E9.5 Sox17^GFP/+ embryos 3 age measurement datum embryonic day 9.5 broker name ArrayExpress common name house mouse life cycle stage embryo stage sample name E-MTAB-2816:E9.5 Sox17^GFP/+ embryos 3 scientific_name Mus musculus strain STOCK Sox17tm2(EGFP/cre)Mgn/Mmnc mouse strain