ERX5142284 E-MTAB-10097:C2_1_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822686 SAMEA8135865 C2_1_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_1 ERX5142285 E-MTAB-10097:C2_10_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822687 SAMEA8135866 C2_10_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_10 ERX5142286 E-MTAB-10097:C2_11_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822688 SAMEA8135867 C2_11_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_11 ERX5142287 E-MTAB-10097:C2_12_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822689 SAMEA8135868 C2_12_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_12 ERX5142288 E-MTAB-10097:C2_13_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822690 SAMEA8135869 C2_13_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_13 ERX5142289 E-MTAB-10097:C2_14_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822691 SAMEA8135870 C2_14_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_14 ERX5142290 E-MTAB-10097:C2_15_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822692 SAMEA8135871 C2_15_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_15 ERX5142291 E-MTAB-10097:C2_16_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822693 SAMEA8135872 C2_16_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_16 ERX5142292 E-MTAB-10097:C2_17_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822694 SAMEA8135873 C2_17_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_17 ERX5142293 E-MTAB-10097:C2_18_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822695 SAMEA8135874 C2_18_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_18 ERX5142294 E-MTAB-10097:C2_19_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822696 SAMEA8135875 C2_19_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_19 ERX5142295 E-MTAB-10097:C2_2_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822697 SAMEA8135876 C2_2_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_2 ERX5142296 E-MTAB-10097:C2_20_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822698 SAMEA8135877 C2_20_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_20 ERX5142297 E-MTAB-10097:C2_21_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822699 SAMEA8135878 C2_21_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_21 ERX5142298 E-MTAB-10097:C2_22_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822700 SAMEA8135879 C2_22_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_22 ERX5142299 E-MTAB-10097:C2_23_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822701 SAMEA8135880 C2_23_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_23 ERX5142300 E-MTAB-10097:C2_24_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822702 SAMEA8135881 C2_24_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_24 ERX5142301 E-MTAB-10097:C2_3_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822703 SAMEA8135882 C2_3_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_3 ERX5142302 E-MTAB-10097:C2_4_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822704 SAMEA8135883 C2_4_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_4 ERX5142303 E-MTAB-10097:C2_5_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822705 SAMEA8135884 C2_5_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_5 ERX5142304 E-MTAB-10097:C2_6_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822706 SAMEA8135885 C2_6_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_6 ERX5142305 E-MTAB-10097:C2_7_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822707 SAMEA8135886 C2_7_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_7 ERX5142306 E-MTAB-10097:C2_8_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822708 SAMEA8135887 C2_8_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_8 ERX5142307 E-MTAB-10097:C2_9_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822709 SAMEA8135888 C2_9_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C2_9 ERX5142308 E-MTAB-10097:C3_1_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822710 SAMEA8135889 C3_1_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_1 ERX5142309 E-MTAB-10097:C3_10_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822711 SAMEA8135890 C3_10_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_10 ERX5142310 E-MTAB-10097:C3_11_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822712 SAMEA8135891 C3_11_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_11 ERX5142311 E-MTAB-10097:C3_12_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822713 SAMEA8135892 C3_12_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_12 ERX5142312 E-MTAB-10097:C3_13_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822714 SAMEA8135893 C3_13_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_13 ERX5142313 E-MTAB-10097:C3_14_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822715 SAMEA8135894 C3_14_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_14 ERX5142314 E-MTAB-10097:C3_15_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822716 SAMEA8135895 C3_15_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_15 ERX5142315 E-MTAB-10097:C3_16_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822717 SAMEA8135896 C3_16_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_16 ERX5142316 E-MTAB-10097:C3_17_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822718 SAMEA8135897 C3_17_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_17 ERX5142317 E-MTAB-10097:C3_18_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822719 SAMEA8135898 C3_18_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_18 ERX5142318 E-MTAB-10097:C3_19_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822720 SAMEA8135899 C3_19_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_19 ERX5142319 E-MTAB-10097:C3_2_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822721 SAMEA8135900 C3_2_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_2 ERX5142320 E-MTAB-10097:C3_20_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822722 SAMEA8135901 C3_20_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_20 ERX5142321 E-MTAB-10097:C3_21_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822723 SAMEA8135902 C3_21_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_21 ERX5142322 E-MTAB-10097:C3_22_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822724 SAMEA8135903 C3_22_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_22 ERX5142323 E-MTAB-10097:C3_23_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822725 SAMEA8135904 C3_23_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_23 ERX5142324 E-MTAB-10097:C3_24_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822726 SAMEA8135905 C3_24_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_24 ERX5142325 E-MTAB-10097:C3_25_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822727 SAMEA8135906 C3_25_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_25 ERX5142326 E-MTAB-10097:C3_26_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822728 SAMEA8135907 C3_26_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_26 ERX5142327 E-MTAB-10097:C3_27_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822729 SAMEA8135908 C3_27_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_27 ERX5142328 E-MTAB-10097:C3_28_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822730 SAMEA8135909 C3_28_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_28 ERX5142329 E-MTAB-10097:C3_29_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822731 SAMEA8135910 C3_29_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_29 ERX5142330 E-MTAB-10097:C3_3_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822732 SAMEA8135911 C3_3_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_3 ERX5142331 E-MTAB-10097:C3_30_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822733 SAMEA8135912 C3_30_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_30 ERX5142332 E-MTAB-10097:C3_31_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822734 SAMEA8135913 C3_31_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_31 ERX5142333 E-MTAB-10097:C3_32_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822735 SAMEA8135914 C3_32_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_32 ERX5142334 E-MTAB-10097:C3_4_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822736 SAMEA8135915 C3_4_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_4 ERX5142335 E-MTAB-10097:C3_5_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822737 SAMEA8135916 C3_5_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_5 ERX5142336 E-MTAB-10097:C3_6_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822738 SAMEA8135917 C3_6_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_6 ERX5142337 E-MTAB-10097:C3_7_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822739 SAMEA8135918 C3_7_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_7 ERX5142338 E-MTAB-10097:C3_8_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822740 SAMEA8135919 C3_8_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_8 ERX5142339 E-MTAB-10097:C3_9_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822741 SAMEA8135920 C3_9_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C3_9 ERX5142340 E-MTAB-10097:C4_1_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822742 SAMEA8135921 C4_1_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_1 ERX5142341 E-MTAB-10097:C4_10_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822743 SAMEA8135922 C4_10_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_10 ERX5142342 E-MTAB-10097:C4_11_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822744 SAMEA8135923 C4_11_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_11 ERX5142343 E-MTAB-10097:C4_12_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822745 SAMEA8135924 C4_12_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_12 ERX5142344 E-MTAB-10097:C4_13_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822746 SAMEA8135925 C4_13_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_13 ERX5142345 E-MTAB-10097:C4_14_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822747 SAMEA8135926 C4_14_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_14 ERX5142346 E-MTAB-10097:C4_15_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822748 SAMEA8135927 C4_15_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_15 ERX5142347 E-MTAB-10097:C4_16_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822749 SAMEA8135928 C4_16_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_16 ERX5142348 E-MTAB-10097:C4_2_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822750 SAMEA8135929 C4_2_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_2 ERX5142349 E-MTAB-10097:C4_3_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822751 SAMEA8135930 C4_3_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_3 ERX5142350 E-MTAB-10097:C4_4_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822752 SAMEA8135931 C4_4_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_4 ERX5142351 E-MTAB-10097:C4_5_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822753 SAMEA8135932 C4_5_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_5 ERX5142352 E-MTAB-10097:C4_6_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822754 SAMEA8135933 C4_6_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_6 ERX5142353 E-MTAB-10097:C4_7_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822755 SAMEA8135934 C4_7_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_7 ERX5142354 E-MTAB-10097:C4_8_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822756 SAMEA8135935 C4_8_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_8 ERX5142355 E-MTAB-10097:C4_9_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822757 SAMEA8135936 C4_9_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C4_9 ERX5142356 E-MTAB-10097:C5_1_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822758 SAMEA8135937 C5_1_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C5_1 ERX5142357 E-MTAB-10097:C5_2_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822759 SAMEA8135938 C5_2_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C5_2 ERX5142358 E-MTAB-10097:C5_3_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822760 SAMEA8135939 C5_3_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C5_3 ERX5142359 E-MTAB-10097:C5_4_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822761 SAMEA8135940 C5_4_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C5_4 ERX5142360 E-MTAB-10097:C5_5_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822762 SAMEA8135941 C5_5_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C5_5 ERX5142361 E-MTAB-10097:C5_6_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822763 SAMEA8135942 C5_6_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C5_6 ERX5142362 E-MTAB-10097:C5_7_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822764 SAMEA8135943 C5_7_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C5_7 ERX5142363 E-MTAB-10097:C5_8_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822765 SAMEA8135944 C5_8_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C5_8 ERX5142364 E-MTAB-10097:C6_1_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822766 SAMEA8135945 C6_1_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_1 ERX5142365 E-MTAB-10097:C6_10_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822767 SAMEA8135946 C6_10_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_10 ERX5142366 E-MTAB-10097:C6_11_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822768 SAMEA8135947 C6_11_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_11 ERX5142367 E-MTAB-10097:C6_12_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822769 SAMEA8135948 C6_12_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_12 ERX5142368 E-MTAB-10097:C6_13_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822770 SAMEA8135949 C6_13_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_13 ERX5142369 E-MTAB-10097:C6_14_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822771 SAMEA8135950 C6_14_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_14 ERX5142370 E-MTAB-10097:C6_15_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822772 SAMEA8135951 C6_15_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_15 ERX5142371 E-MTAB-10097:C6_16_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822773 SAMEA8135952 C6_16_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_16 ERX5142372 E-MTAB-10097:C6_2_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822774 SAMEA8135953 C6_2_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_2 ERX5142373 E-MTAB-10097:C6_3_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822775 SAMEA8135954 C6_3_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_3 ERX5142374 E-MTAB-10097:C6_4_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822776 SAMEA8135955 C6_4_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_4 ERX5142375 E-MTAB-10097:C6_5_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822777 SAMEA8135956 C6_5_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_5 ERX5142376 E-MTAB-10097:C6_6_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822778 SAMEA8135957 C6_6_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_6 ERX5142377 E-MTAB-10097:C6_7_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822779 SAMEA8135958 C6_7_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_7 ERX5142378 E-MTAB-10097:C6_8_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822780 SAMEA8135959 C6_8_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_8 ERX5142379 E-MTAB-10097:C6_9_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822781 SAMEA8135960 C6_9_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C6_9 ERX5142380 E-MTAB-10097:C7_1_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822782 SAMEA8135961 C7_1_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_1 ERX5142381 E-MTAB-10097:C7_10_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822783 SAMEA8135962 C7_10_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_10 ERX5142382 E-MTAB-10097:C7_11_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822784 SAMEA8135963 C7_11_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_11 ERX5142383 E-MTAB-10097:C7_12_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822785 SAMEA8135964 C7_12_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_12 ERX5142384 E-MTAB-10097:C7_13_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822786 SAMEA8135965 C7_13_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_13 ERX5142385 E-MTAB-10097:C7_14_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822787 SAMEA8135966 C7_14_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_14 ERX5142386 E-MTAB-10097:C7_15_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822788 SAMEA8135967 C7_15_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_15 ERX5142387 E-MTAB-10097:C7_16_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822789 SAMEA8135968 C7_16_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_16 ERX5142388 E-MTAB-10097:C7_17_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822790 SAMEA8135969 C7_17_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_17 ERX5142389 E-MTAB-10097:C7_18_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822791 SAMEA8135970 C7_18_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_18 ERX5142390 E-MTAB-10097:C7_19_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822792 SAMEA8135971 C7_19_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_19 ERX5142391 E-MTAB-10097:C7_2_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822793 SAMEA8135972 C7_2_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_2 ERX5142392 E-MTAB-10097:C7_20_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822794 SAMEA8135973 C7_20_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_20 ERX5142393 E-MTAB-10097:C7_21_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822795 SAMEA8135974 C7_21_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_21 ERX5142394 E-MTAB-10097:C7_22_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822796 SAMEA8135975 C7_22_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_22 ERX5142395 E-MTAB-10097:C7_23_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822797 SAMEA8135976 C7_23_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_23 ERX5142396 E-MTAB-10097:C7_24_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822798 SAMEA8135977 C7_24_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_24 ERX5142397 E-MTAB-10097:C7_3_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822799 SAMEA8135978 C7_3_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_3 ERX5142398 E-MTAB-10097:C7_4_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822800 SAMEA8135979 C7_4_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_4 ERX5142399 E-MTAB-10097:C7_5_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822801 SAMEA8135980 C7_5_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_5 ERX5142400 E-MTAB-10097:C7_6_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822802 SAMEA8135981 C7_6_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_6 ERX5142401 E-MTAB-10097:C7_7_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822803 SAMEA8135982 C7_7_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_7 ERX5142402 E-MTAB-10097:C7_8_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822804 SAMEA8135983 C7_8_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_8 ERX5142403 E-MTAB-10097:C7_9_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822805 SAMEA8135984 C7_9_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C7_9 ERX5142404 E-MTAB-10097:C8_1_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822806 SAMEA8135985 C8_1_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_1 ERX5142405 E-MTAB-10097:C8_10_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822807 SAMEA8135986 C8_10_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_10 ERX5142406 E-MTAB-10097:C8_11_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822808 SAMEA8135987 C8_11_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_11 ERX5142407 E-MTAB-10097:C8_12_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822809 SAMEA8135988 C8_12_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_12 ERX5142408 E-MTAB-10097:C8_13_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822810 SAMEA8135989 C8_13_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_13 ERX5142409 E-MTAB-10097:C8_14_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822811 SAMEA8135990 C8_14_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_14 ERX5142410 E-MTAB-10097:C8_15_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822812 SAMEA8135991 C8_15_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_15 ERX5142411 E-MTAB-10097:C8_16_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822813 SAMEA8135992 C8_16_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_16 ERX5142412 E-MTAB-10097:C8_17_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822814 SAMEA8135993 C8_17_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_17 ERX5142413 E-MTAB-10097:C8_18_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822815 SAMEA8135994 C8_18_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_18 ERX5142414 E-MTAB-10097:C8_19_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822816 SAMEA8135995 C8_19_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_19 ERX5142415 E-MTAB-10097:C8_2_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822817 SAMEA8135996 C8_2_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_2 ERX5142416 E-MTAB-10097:C8_20_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822818 SAMEA8135997 C8_20_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_20 ERX5142417 E-MTAB-10097:C8_21_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822819 SAMEA8135998 C8_21_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_21 ERX5142418 E-MTAB-10097:C8_22_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822820 SAMEA8135999 C8_22_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_22 ERX5142419 E-MTAB-10097:C8_23_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822821 SAMEA8136000 C8_23_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_23 ERX5142420 E-MTAB-10097:C8_24_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822822 SAMEA8136001 C8_24_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_24 ERX5142421 E-MTAB-10097:C8_3_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822823 SAMEA8136002 C8_3_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_3 ERX5142422 E-MTAB-10097:C8_4_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822824 SAMEA8136003 C8_4_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_4 ERX5142423 E-MTAB-10097:C8_5_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822825 SAMEA8136004 C8_5_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_5 ERX5142424 E-MTAB-10097:C8_6_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822826 SAMEA8136005 C8_6_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_6 ERX5142425 E-MTAB-10097:C8_7_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822827 SAMEA8136006 C8_7_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_7 ERX5142426 E-MTAB-10097:C8_8_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822828 SAMEA8136007 C8_8_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_8 ERX5142427 E-MTAB-10097:C8_9_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822829 SAMEA8136008 C8_9_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C8_9 ERX5142428 E-MTAB-10097:C9_1_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822830 SAMEA8136009 C9_1_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_1 ERX5142429 E-MTAB-10097:C9_10_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822831 SAMEA8136010 C9_10_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_10 ERX5142430 E-MTAB-10097:C9_11_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822832 SAMEA8136011 C9_11_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_11 ERX5142431 E-MTAB-10097:C9_12_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822833 SAMEA8136012 C9_12_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_12 ERX5142432 E-MTAB-10097:C9_13_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822834 SAMEA8136013 C9_13_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_13 ERX5142433 E-MTAB-10097:C9_14_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822835 SAMEA8136014 C9_14_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_14 ERX5142434 E-MTAB-10097:C9_15_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822836 SAMEA8136015 C9_15_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_15 ERX5142435 E-MTAB-10097:C9_16_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822837 SAMEA8136016 C9_16_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_16 ERX5142436 E-MTAB-10097:C9_17_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822838 SAMEA8136017 C9_17_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_17 ERX5142437 E-MTAB-10097:C9_18_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822839 SAMEA8136018 C9_18_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_18 ERX5142438 E-MTAB-10097:C9_19_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822840 SAMEA8136019 C9_19_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_19 ERX5142439 E-MTAB-10097:C9_2_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822841 SAMEA8136020 C9_2_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_2 ERX5142440 E-MTAB-10097:C9_20_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822842 SAMEA8136021 C9_20_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_20 ERX5142441 E-MTAB-10097:C9_21_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822843 SAMEA8136022 C9_21_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_21 ERX5142442 E-MTAB-10097:C9_22_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822844 SAMEA8136023 C9_22_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_22 ERX5142443 E-MTAB-10097:C9_23_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822845 SAMEA8136024 C9_23_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_23 ERX5142444 E-MTAB-10097:C9_24_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822846 SAMEA8136025 C9_24_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_24 ERX5142445 E-MTAB-10097:C9_3_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822847 SAMEA8136026 C9_3_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_3 ERX5142446 E-MTAB-10097:C9_4_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822848 SAMEA8136027 C9_4_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_4 ERX5142447 E-MTAB-10097:C9_5_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822849 SAMEA8136028 C9_5_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_5 ERX5142448 E-MTAB-10097:C9_6_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822850 SAMEA8136029 C9_6_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_6 ERX5142449 E-MTAB-10097:C9_7_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822851 SAMEA8136030 C9_7_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_7 ERX5142450 E-MTAB-10097:C9_8_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822852 SAMEA8136031 C9_8_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_8 ERX5142451 E-MTAB-10097:C9_9_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822853 SAMEA8136032 C9_9_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound none Experimental Factor: single cell identifier C9_9 ERX5142452 E-MTAB-10097:D10_1_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822854 SAMEA8136033 D10_1_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_1 ERX5142453 E-MTAB-10097:D10_10_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822855 SAMEA8136034 D10_10_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_10 ERX5142454 E-MTAB-10097:D10_11_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822856 SAMEA8136035 D10_11_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_11 ERX5142455 E-MTAB-10097:D10_12_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822857 SAMEA8136036 D10_12_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_12 ERX5142456 E-MTAB-10097:D10_13_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822858 SAMEA8136037 D10_13_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_13 ERX5142457 E-MTAB-10097:D10_14_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822859 SAMEA8136038 D10_14_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_14 ERX5142458 E-MTAB-10097:D10_15_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822860 SAMEA8136039 D10_15_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_15 ERX5142459 E-MTAB-10097:D10_16_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822861 SAMEA8136040 D10_16_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_16 ERX5142460 E-MTAB-10097:D10_17_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822862 SAMEA8136041 D10_17_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_17 ERX5142461 E-MTAB-10097:D10_18_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822863 SAMEA8136042 D10_18_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_18 ERX5142462 E-MTAB-10097:D10_19_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822864 SAMEA8136043 D10_19_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_19 ERX5142463 E-MTAB-10097:D10_2_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822865 SAMEA8136044 D10_2_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_2 ERX5142464 E-MTAB-10097:D10_20_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822866 SAMEA8136045 D10_20_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_20 ERX5142465 E-MTAB-10097:D10_21_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822867 SAMEA8136046 D10_21_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_21 ERX5142466 E-MTAB-10097:D10_22_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822868 SAMEA8136047 D10_22_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_22 ERX5142467 E-MTAB-10097:D10_23_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822869 SAMEA8136048 D10_23_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_23 ERX5142468 E-MTAB-10097:D10_24_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822870 SAMEA8136049 D10_24_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_24 ERX5142469 E-MTAB-10097:D10_25_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822871 SAMEA8136050 D10_25_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_25 ERX5142470 E-MTAB-10097:D10_26_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822872 SAMEA8136051 D10_26_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_26 ERX5142471 E-MTAB-10097:D10_27_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822873 SAMEA8136052 D10_27_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_27 ERX5142472 E-MTAB-10097:D10_28_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822874 SAMEA8136053 D10_28_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_28 ERX5142473 E-MTAB-10097:D10_29_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822875 SAMEA8136054 D10_29_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_29 ERX5142474 E-MTAB-10097:D10_3_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822876 SAMEA8136055 D10_3_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_3 ERX5142475 E-MTAB-10097:D10_30_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822877 SAMEA8136056 D10_30_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_30 ERX5142476 E-MTAB-10097:D10_31_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822878 SAMEA8136057 D10_31_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_31 ERX5142477 E-MTAB-10097:D10_32_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822879 SAMEA8136058 D10_32_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_32 ERX5142478 E-MTAB-10097:D10_33_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822880 SAMEA8136059 D10_33_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_33 ERX5142479 E-MTAB-10097:D10_34_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822881 SAMEA8136060 D10_34_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_34 ERX5142480 E-MTAB-10097:D10_35_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822882 SAMEA8136061 D10_35_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_35 ERX5142481 E-MTAB-10097:D10_36_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822883 SAMEA8136062 D10_36_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_36 ERX5142482 E-MTAB-10097:D10_37_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822884 SAMEA8136063 D10_37_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_37 ERX5142483 E-MTAB-10097:D10_38_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822885 SAMEA8136064 D10_38_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_38 ERX5142484 E-MTAB-10097:D10_39_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822886 SAMEA8136065 D10_39_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_39 ERX5142485 E-MTAB-10097:D10_4_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822887 SAMEA8136066 D10_4_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_4 ERX5142486 E-MTAB-10097:D10_40_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822888 SAMEA8136067 D10_40_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_40 ERX5142487 E-MTAB-10097:D10_41_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822889 SAMEA8136068 D10_41_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_41 ERX5142488 E-MTAB-10097:D10_42_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822890 SAMEA8136069 D10_42_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_42 ERX5142489 E-MTAB-10097:D10_43_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822891 SAMEA8136070 D10_43_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_43 ERX5142490 E-MTAB-10097:D10_44_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822892 SAMEA8136071 D10_44_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_44 ERX5142491 E-MTAB-10097:D10_45_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822893 SAMEA8136072 D10_45_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_45 ERX5142492 E-MTAB-10097:D10_46_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822894 SAMEA8136073 D10_46_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_46 ERX5142493 E-MTAB-10097:D10_47_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822895 SAMEA8136074 D10_47_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_47 ERX5142494 E-MTAB-10097:D10_48_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822896 SAMEA8136075 D10_48_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_48 ERX5142495 E-MTAB-10097:D10_49_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822897 SAMEA8136076 D10_49_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_49 ERX5142496 E-MTAB-10097:D10_5_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822898 SAMEA8136077 D10_5_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_5 ERX5142497 E-MTAB-10097:D10_50_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822899 SAMEA8136078 D10_50_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_50 ERX5142498 E-MTAB-10097:D10_51_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822900 SAMEA8136079 D10_51_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_51 ERX5142499 E-MTAB-10097:D10_52_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822901 SAMEA8136080 D10_52_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_52 ERX5142500 E-MTAB-10097:D10_53_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822902 SAMEA8136081 D10_53_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_53 ERX5142501 E-MTAB-10097:D10_54_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822903 SAMEA8136082 D10_54_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_54 ERX5142502 E-MTAB-10097:D10_55_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822904 SAMEA8136083 D10_55_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_55 ERX5142503 E-MTAB-10097:D10_56_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822905 SAMEA8136084 D10_56_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_56 ERX5142504 E-MTAB-10097:D10_57_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822906 SAMEA8136085 D10_57_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_57 ERX5142505 E-MTAB-10097:D10_58_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822907 SAMEA8136086 D10_58_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_58 ERX5142506 E-MTAB-10097:D10_59_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822908 SAMEA8136087 D10_59_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_59 ERX5142507 E-MTAB-10097:D10_6_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822909 SAMEA8136088 D10_6_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_6 ERX5142508 E-MTAB-10097:D10_60_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822910 SAMEA8136089 D10_60_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_60 ERX5142509 E-MTAB-10097:D10_61_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822911 SAMEA8136090 D10_61_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_61 ERX5142510 E-MTAB-10097:D10_62_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822912 SAMEA8136091 D10_62_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_62 ERX5142511 E-MTAB-10097:D10_63_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822913 SAMEA8136092 D10_63_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_63 ERX5142512 E-MTAB-10097:D10_7_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822914 SAMEA8136093 D10_7_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_7 ERX5142513 E-MTAB-10097:D10_8_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822915 SAMEA8136094 D10_8_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_8 ERX5142514 E-MTAB-10097:D10_9_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822916 SAMEA8136095 D10_9_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D10_9 ERX5142515 E-MTAB-10097:D11_1_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822917 SAMEA8136096 D11_1_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_1 ERX5142516 E-MTAB-10097:D11_10_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822918 SAMEA8136097 D11_10_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_10 ERX5142517 E-MTAB-10097:D11_11_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822919 SAMEA8136098 D11_11_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_11 ERX5142518 E-MTAB-10097:D11_12_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822920 SAMEA8136099 D11_12_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_12 ERX5142519 E-MTAB-10097:D11_13_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822921 SAMEA8136100 D11_13_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_13 ERX5142520 E-MTAB-10097:D11_14_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822922 SAMEA8136101 D11_14_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_14 ERX5142521 E-MTAB-10097:D11_15_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822923 SAMEA8136102 D11_15_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_15 ERX5142522 E-MTAB-10097:D11_16_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822924 SAMEA8136103 D11_16_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_16 ERX5142523 E-MTAB-10097:D11_17_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822925 SAMEA8136104 D11_17_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_17 ERX5142524 E-MTAB-10097:D11_18_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822926 SAMEA8136105 D11_18_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_18 ERX5142525 E-MTAB-10097:D11_19_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822927 SAMEA8136106 D11_19_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_19 ERX5142526 E-MTAB-10097:D11_2_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822928 SAMEA8136107 D11_2_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_2 ERX5142527 E-MTAB-10097:D11_20_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822929 SAMEA8136108 D11_20_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_20 ERX5142528 E-MTAB-10097:D11_21_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822930 SAMEA8136109 D11_21_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_21 ERX5142529 E-MTAB-10097:D11_22_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822931 SAMEA8136110 D11_22_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_22 ERX5142530 E-MTAB-10097:D11_23_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822932 SAMEA8136111 D11_23_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_23 ERX5142531 E-MTAB-10097:D11_24_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822933 SAMEA8136112 D11_24_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_24 ERX5142532 E-MTAB-10097:D11_25_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822934 SAMEA8136113 D11_25_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_25 ERX5142533 E-MTAB-10097:D11_26_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822935 SAMEA8136114 D11_26_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_26 ERX5142534 E-MTAB-10097:D11_27_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822936 SAMEA8136115 D11_27_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_27 ERX5142535 E-MTAB-10097:D11_28_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822937 SAMEA8136116 D11_28_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_28 ERX5142536 E-MTAB-10097:D11_29_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822938 SAMEA8136117 D11_29_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_29 ERX5142537 E-MTAB-10097:D11_3_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822939 SAMEA8136118 D11_3_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_3 ERX5142538 E-MTAB-10097:D11_30_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822940 SAMEA8136119 D11_30_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_30 ERX5142539 E-MTAB-10097:D11_31_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822941 SAMEA8136120 D11_31_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_31 ERX5142540 E-MTAB-10097:D11_32_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822942 SAMEA8136121 D11_32_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_32 ERX5142541 E-MTAB-10097:D11_4_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822943 SAMEA8136122 D11_4_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_4 ERX5142542 E-MTAB-10097:D11_5_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822944 SAMEA8136123 D11_5_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_5 ERX5142543 E-MTAB-10097:D11_6_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822945 SAMEA8136124 D11_6_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_6 ERX5142544 E-MTAB-10097:D11_7_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822946 SAMEA8136125 D11_7_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_7 ERX5142545 E-MTAB-10097:D11_8_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822947 SAMEA8136126 D11_8_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_8 ERX5142546 E-MTAB-10097:D11_9_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822948 SAMEA8136127 D11_9_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D11_9 ERX5142547 E-MTAB-10097:D12_1_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822949 SAMEA8136128 D12_1_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_1 ERX5142548 E-MTAB-10097:D12_10_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822950 SAMEA8136129 D12_10_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_10 ERX5142549 E-MTAB-10097:D12_11_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822951 SAMEA8136130 D12_11_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_11 ERX5142550 E-MTAB-10097:D12_12_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822952 SAMEA8136131 D12_12_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_12 ERX5142551 E-MTAB-10097:D12_13_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822953 SAMEA8136132 D12_13_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_13 ERX5142552 E-MTAB-10097:D12_14_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822954 SAMEA8136133 D12_14_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_14 ERX5142553 E-MTAB-10097:D12_15_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822955 SAMEA8136134 D12_15_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_15 ERX5142554 E-MTAB-10097:D12_16_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822956 SAMEA8136135 D12_16_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_16 ERX5142555 E-MTAB-10097:D12_17_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822957 SAMEA8136136 D12_17_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_17 ERX5142556 E-MTAB-10097:D12_18_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822958 SAMEA8136137 D12_18_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_18 ERX5142557 E-MTAB-10097:D12_19_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822959 SAMEA8136138 D12_19_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_19 ERX5142558 E-MTAB-10097:D12_2_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822960 SAMEA8136139 D12_2_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_2 ERX5142559 E-MTAB-10097:D12_20_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822961 SAMEA8136140 D12_20_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_20 ERX5142560 E-MTAB-10097:D12_21_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822962 SAMEA8136141 D12_21_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_21 ERX5142561 E-MTAB-10097:D12_22_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822963 SAMEA8136142 D12_22_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_22 ERX5142562 E-MTAB-10097:D12_23_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822964 SAMEA8136143 D12_23_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_23 ERX5142563 E-MTAB-10097:D12_24_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822965 SAMEA8136144 D12_24_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_24 ERX5142564 E-MTAB-10097:D12_25_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822966 SAMEA8136145 D12_25_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_25 ERX5142565 E-MTAB-10097:D12_26_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822967 SAMEA8136146 D12_26_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_26 ERX5142566 E-MTAB-10097:D12_27_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822968 SAMEA8136147 D12_27_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_27 ERX5142567 E-MTAB-10097:D12_28_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822969 SAMEA8136148 D12_28_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_28 ERX5142568 E-MTAB-10097:D12_29_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822970 SAMEA8136149 D12_29_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_29 ERX5142569 E-MTAB-10097:D12_3_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822971 SAMEA8136150 D12_3_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_3 ERX5142570 E-MTAB-10097:D12_30_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822972 SAMEA8136151 D12_30_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_30 ERX5142571 E-MTAB-10097:D12_31_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822973 SAMEA8136152 D12_31_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_31 ERX5142572 E-MTAB-10097:D12_32_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822974 SAMEA8136153 D12_32_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_32 ERX5142573 E-MTAB-10097:D12_4_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822975 SAMEA8136154 D12_4_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_4 ERX5142574 E-MTAB-10097:D12_5_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822976 SAMEA8136155 D12_5_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_5 ERX5142575 E-MTAB-10097:D12_6_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822977 SAMEA8136156 D12_6_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_6 ERX5142576 E-MTAB-10097:D12_7_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822978 SAMEA8136157 D12_7_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_7 ERX5142577 E-MTAB-10097:D12_8_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822979 SAMEA8136158 D12_8_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_8 ERX5142578 E-MTAB-10097:D12_9_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822980 SAMEA8136159 D12_9_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D12_9 ERX5142579 E-MTAB-10097:D8_1_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822981 SAMEA8136160 D8_1_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_1 ERX5142580 E-MTAB-10097:D8_10_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822982 SAMEA8136161 D8_10_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_10 ERX5142581 E-MTAB-10097:D8_11_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822983 SAMEA8136162 D8_11_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_11 ERX5142582 E-MTAB-10097:D8_12_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822984 SAMEA8136163 D8_12_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_12 ERX5142583 E-MTAB-10097:D8_13_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822985 SAMEA8136164 D8_13_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_13 ERX5142584 E-MTAB-10097:D8_14_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822986 SAMEA8136165 D8_14_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_14 ERX5142585 E-MTAB-10097:D8_15_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822987 SAMEA8136166 D8_15_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_15 ERX5142586 E-MTAB-10097:D8_16_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822988 SAMEA8136167 D8_16_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_16 ERX5142587 E-MTAB-10097:D8_17_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822989 SAMEA8136168 D8_17_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_17 ERX5142588 E-MTAB-10097:D8_18_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822990 SAMEA8136169 D8_18_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_18 ERX5142589 E-MTAB-10097:D8_19_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822991 SAMEA8136170 D8_19_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_19 ERX5142590 E-MTAB-10097:D8_2_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822992 SAMEA8136171 D8_2_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_2 ERX5142591 E-MTAB-10097:D8_20_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822993 SAMEA8136172 D8_20_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_20 ERX5142592 E-MTAB-10097:D8_21_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822994 SAMEA8136173 D8_21_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_21 ERX5142593 E-MTAB-10097:D8_22_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822995 SAMEA8136174 D8_22_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_22 ERX5142594 E-MTAB-10097:D8_23_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822996 SAMEA8136175 D8_23_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_23 ERX5142595 E-MTAB-10097:D8_24_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822997 SAMEA8136176 D8_24_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_24 ERX5142596 E-MTAB-10097:D8_25_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822998 SAMEA8136177 D8_25_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_25 ERX5142597 E-MTAB-10097:D8_26_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5822999 SAMEA8136178 D8_26_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_26 ERX5142598 E-MTAB-10097:D8_27_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823000 SAMEA8136179 D8_27_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_27 ERX5142599 E-MTAB-10097:D8_28_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823001 SAMEA8136180 D8_28_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_28 ERX5142600 E-MTAB-10097:D8_29_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823002 SAMEA8136181 D8_29_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_29 ERX5142601 E-MTAB-10097:D8_3_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823003 SAMEA8136182 D8_3_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_3 ERX5142602 E-MTAB-10097:D8_30_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823004 SAMEA8136183 D8_30_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_30 ERX5142603 E-MTAB-10097:D8_31_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823005 SAMEA8136184 D8_31_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_31 ERX5142604 E-MTAB-10097:D8_32_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823006 SAMEA8136185 D8_32_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_32 ERX5142605 E-MTAB-10097:D8_4_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823007 SAMEA8136186 D8_4_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_4 ERX5142606 E-MTAB-10097:D8_5_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823008 SAMEA8136187 D8_5_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_5 ERX5142607 E-MTAB-10097:D8_6_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823009 SAMEA8136188 D8_6_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_6 ERX5142608 E-MTAB-10097:D8_7_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823010 SAMEA8136189 D8_7_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_7 ERX5142609 E-MTAB-10097:D8_8_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823011 SAMEA8136190 D8_8_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_8 ERX5142610 E-MTAB-10097:D8_9_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823012 SAMEA8136191 D8_9_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D8_9 ERX5142611 E-MTAB-10097:D9_1_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823013 SAMEA8136192 D9_1_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_1 ERX5142612 E-MTAB-10097:D9_10_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823014 SAMEA8136193 D9_10_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_10 ERX5142613 E-MTAB-10097:D9_11_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823015 SAMEA8136194 D9_11_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_11 ERX5142614 E-MTAB-10097:D9_12_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823016 SAMEA8136195 D9_12_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_12 ERX5142615 E-MTAB-10097:D9_13_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823017 SAMEA8136196 D9_13_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_13 ERX5142616 E-MTAB-10097:D9_14_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823018 SAMEA8136197 D9_14_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_14 ERX5142617 E-MTAB-10097:D9_15_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823019 SAMEA8136198 D9_15_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_15 ERX5142618 E-MTAB-10097:D9_16_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823020 SAMEA8136199 D9_16_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_16 ERX5142619 E-MTAB-10097:D9_17_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823021 SAMEA8136200 D9_17_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_17 ERX5142620 E-MTAB-10097:D9_18_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823022 SAMEA8136201 D9_18_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_18 ERX5142621 E-MTAB-10097:D9_19_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823023 SAMEA8136202 D9_19_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_19 ERX5142622 E-MTAB-10097:D9_2_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823024 SAMEA8136203 D9_2_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_2 ERX5142623 E-MTAB-10097:D9_20_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823025 SAMEA8136204 D9_20_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_20 ERX5142624 E-MTAB-10097:D9_21_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823026 SAMEA8136205 D9_21_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_21 ERX5142625 E-MTAB-10097:D9_22_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823027 SAMEA8136206 D9_22_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_22 ERX5142626 E-MTAB-10097:D9_23_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823028 SAMEA8136207 D9_23_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_23 ERX5142627 E-MTAB-10097:D9_24_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823029 SAMEA8136208 D9_24_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_24 ERX5142628 E-MTAB-10097:D9_25_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823030 SAMEA8136209 D9_25_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_25 ERX5142629 E-MTAB-10097:D9_26_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823031 SAMEA8136210 D9_26_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_26 ERX5142630 E-MTAB-10097:D9_27_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823032 SAMEA8136211 D9_27_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_27 ERX5142631 E-MTAB-10097:D9_28_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823033 SAMEA8136212 D9_28_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_28 ERX5142632 E-MTAB-10097:D9_29_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823034 SAMEA8136213 D9_29_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_29 ERX5142633 E-MTAB-10097:D9_3_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823035 SAMEA8136214 D9_3_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_3 ERX5142634 E-MTAB-10097:D9_30_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823036 SAMEA8136215 D9_30_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_30 ERX5142635 E-MTAB-10097:D9_31_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823037 SAMEA8136216 D9_31_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_31 ERX5142636 E-MTAB-10097:D9_32_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823038 SAMEA8136217 D9_32_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_32 ERX5142637 E-MTAB-10097:D9_4_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823039 SAMEA8136218 D9_4_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_4 ERX5142638 E-MTAB-10097:D9_5_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823040 SAMEA8136219 D9_5_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_5 ERX5142639 E-MTAB-10097:D9_6_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823041 SAMEA8136220 D9_6_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_6 ERX5142640 E-MTAB-10097:D9_7_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823042 SAMEA8136221 D9_7_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_7 ERX5142641 E-MTAB-10097:D9_8_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823043 SAMEA8136222 D9_8_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_8 ERX5142642 E-MTAB-10097:D9_9_p ERP127251 PRJEB43299 Single Cell Multi-Omics of Human Preimplantation Embryos Demonstrates Susceptibility to Excess Glucocorticoid Exposure(BSseq part) ERS5823044 SAMEA8136223 D9_9_p Bisulfite-Seq GENOMIC SINGLE CELL RANDOM Human embryos were obtained the Huddinge Karolinska Hospital with ethical approval from regional ethics board (2018/691-31). Embryos were dissociated through trituration in TrypLE, (Life technologies) and handpicked with fine glass capillaries. Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5% O2 in IVF plates containing 700μl of CCMTM media (Vitrolife) covered with 300μl of OvoilTM (Vitrolife) Embryos were acquired fresh (not vitrified) from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and were cultured until E7 (expanded blastocyst), supplemented with glucocorticoid (dexamethasone, 0.1µg/ml (2.5µM)). Cells were directly dispensed in lysis buffer prepared according to a modified version of the snMT-seq protocol The cDNA libraries were generated using Smart-seq2 as previously described.The quantity and quality of the cDNA, small ncRNA and DNA methylation libraries were assessed using an Agilent 2100 BioAnalyzer (Agilent Technologies). Illumina HiSeq 2000 Experimental Factor: compound dexamethasone Experimental Factor: dose 2.5 Experimental Factor: single cell identifier D9_9