ERX5229603 E-MTAB-10197:Replicate 1_p ERP127562 PRJEB43593 Gremlin 1+ fibroblastic niche maintains dendritic cell homeostasis in lymphoid tissues ERS5915022 SAMEA8228452 Replicate 1_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA Skin draining lymph nodes (inguinal, axillary and brachial, unless otherwise noted) were collected and incubated in RPMI medium containing 2% FBS, 0.1mg/ml DNAse I (Invitrogen), 0.2mg/ml collagenase P (Roche) and 0.8 mg/ml Dispase (Roche). Sorted cells were loaded onto a 10x Chromium Chip using reagents from the Chromium Single Cell 3' Library Kit (10x Genomics) according to the manufacturer's protocol. Amplified cDNA was used for 3' RNA-seq library generation, RNA-seq libraries were prepared following themanufacturer's user guide (10x Genomics). Illumina HiSeq 4000 Experimental Factor: replicate 1 ERX5229604 E-MTAB-10197:Replicate 2_p ERP127562 PRJEB43593 Gremlin 1+ fibroblastic niche maintains dendritic cell homeostasis in lymphoid tissues ERS5915023 SAMEA8228453 Replicate 2_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PolyA Skin draining lymph nodes (inguinal, axillary and brachial, unless otherwise noted) were collected and incubated in RPMI medium containing 2% FBS, 0.1mg/ml DNAse I (Invitrogen), 0.2mg/ml collagenase P (Roche) and 0.8 mg/ml Dispase (Roche). Sorted cells were loaded onto a 10x Chromium Chip using reagents from the Chromium Single Cell 3' Library Kit (10x Genomics) according to the manufacturer's protocol. Amplified cDNA was used for 3' RNA-seq library generation, RNA-seq libraries were prepared following themanufacturer's user guide (10x Genomics). Illumina HiSeq 4000 Experimental Factor: replicate 2