ERX682264 E-MTAB-3261:PolyA+_kidney E-MTAB-3261:PolyA+_kidney ERP009351 E-MTAB-3261 GENCODE PCR-Seq Batch M1 ERS643859 SAMEA3216853 E-MTAB-3261:PolyA+_kidney PolyA+_kidney AMPLICON TRANSCRIPTOMIC RT-PCR An 8-week-old C57/BL6 mouse purchased from Charles River was sacrificed, from which brain, heart, kidney, liver, lung, muscle (biceps femoris), spleen and testis were dissected. The tissues were homogenised in Trizol and total RNA was extracted. Any remaining DNA was removed with the Qiagen RNase-free DNase kit (ref. 79254) and total RNA was cleaned up with a Qiagen RNeasy mini kit (ref. 74104) column. Then, poly-A+ RNA was extracted from the total RNA using the Qiagen Oligotex mRNA mini kit (ref. 70022). Reverse transcription was performed with the Invitrogen SuperScript III first-strand synthesis kit (ref. 18080-051) using random hexamers for priming. PCR amplifications were performed using 0.2 ng of initial poly A+ RNA (as measured before doing the reverse transcription) in a final volume of 12.5 uL with JumpStart REDTaq ReadyMix (Sigma-Aldrich) and a primer concentration of 0.4 uM. Reactions took place in 384-well plates format on an automatized Evoware platform (TECAN) combined with a Tetrad2 thermocycler (Bio-Rad) that allows processing four plates in parallel. Because monoexonic amplification is sensitive to genomic DNA contaminations, monoexonic models were assessed by amplification of cDNA in which a dNTP analog was incorporated using the mRNA Selective PCR Kit (TAKARA). Illumina HiSeq 2000 Experimental Factor: organism part kidney LIBRARY_STRAND not applicable ERX682265 E-MTAB-3261:PolyA+_liver E-MTAB-3261:PolyA+_liver ERP009351 E-MTAB-3261 GENCODE PCR-Seq Batch M1 ERS643860 SAMEA3216854 E-MTAB-3261:PolyA+_liver PolyA+_liver AMPLICON TRANSCRIPTOMIC RT-PCR An 8-week-old C57/BL6 mouse purchased from Charles River was sacrificed, from which brain, heart, kidney, liver, lung, muscle (biceps femoris), spleen and testis were dissected. The tissues were homogenised in Trizol and total RNA was extracted. Any remaining DNA was removed with the Qiagen RNase-free DNase kit (ref. 79254) and total RNA was cleaned up with a Qiagen RNeasy mini kit (ref. 74104) column. Then, poly-A+ RNA was extracted from the total RNA using the Qiagen Oligotex mRNA mini kit (ref. 70022). Reverse transcription was performed with the Invitrogen SuperScript III first-strand synthesis kit (ref. 18080-051) using random hexamers for priming. PCR amplifications were performed using 0.2 ng of initial poly A+ RNA (as measured before doing the reverse transcription) in a final volume of 12.5 uL with JumpStart REDTaq ReadyMix (Sigma-Aldrich) and a primer concentration of 0.4 uM. Reactions took place in 384-well plates format on an automatized Evoware platform (TECAN) combined with a Tetrad2 thermocycler (Bio-Rad) that allows processing four plates in parallel. Because monoexonic amplification is sensitive to genomic DNA contaminations, monoexonic models were assessed by amplification of cDNA in which a dNTP analog was incorporated using the mRNA Selective PCR Kit (TAKARA). Illumina HiSeq 2000 Experimental Factor: organism part liver LIBRARY_STRAND not applicable ERX682258 E-MTAB-3261:PolyA+_brain E-MTAB-3261:PolyA+_brain ERP009351 E-MTAB-3261 GENCODE PCR-Seq Batch M1 ERS643853 SAMEA3216847 E-MTAB-3261:PolyA+_brain PolyA+_brain AMPLICON TRANSCRIPTOMIC RT-PCR An 8-week-old C57/BL6 mouse purchased from Charles River was sacrificed, from which brain, heart, kidney, liver, lung, muscle (biceps femoris), spleen and testis were dissected. The tissues were homogenised in Trizol and total RNA was extracted. Any remaining DNA was removed with the Qiagen RNase-free DNase kit (ref. 79254) and total RNA was cleaned up with a Qiagen RNeasy mini kit (ref. 74104) column. Then, poly-A+ RNA was extracted from the total RNA using the Qiagen Oligotex mRNA mini kit (ref. 70022). Reverse transcription was performed with the Invitrogen SuperScript III first-strand synthesis kit (ref. 18080-051) using random hexamers for priming. PCR amplifications were performed using 0.2 ng of initial poly A+ RNA (as measured before doing the reverse transcription) in a final volume of 12.5 uL with JumpStart REDTaq ReadyMix (Sigma-Aldrich) and a primer concentration of 0.4 uM. Reactions took place in 384-well plates format on an automatized Evoware platform (TECAN) combined with a Tetrad2 thermocycler (Bio-Rad) that allows processing four plates in parallel. Because monoexonic amplification is sensitive to genomic DNA contaminations, monoexonic models were assessed by amplification of cDNA in which a dNTP analog was incorporated using the mRNA Selective PCR Kit (TAKARA). Illumina HiSeq 2000 Experimental Factor: organism part brain LIBRARY_STRAND not applicable ERX682259 E-MTAB-3261:PolyA+_spleen E-MTAB-3261:PolyA+_spleen ERP009351 E-MTAB-3261 GENCODE PCR-Seq Batch M1 ERS643854 SAMEA3216848 E-MTAB-3261:PolyA+_spleen PolyA+_spleen AMPLICON TRANSCRIPTOMIC RT-PCR An 8-week-old C57/BL6 mouse purchased from Charles River was sacrificed, from which brain, heart, kidney, liver, lung, muscle (biceps femoris), spleen and testis were dissected. The tissues were homogenised in Trizol and total RNA was extracted. Any remaining DNA was removed with the Qiagen RNase-free DNase kit (ref. 79254) and total RNA was cleaned up with a Qiagen RNeasy mini kit (ref. 74104) column. Then, poly-A+ RNA was extracted from the total RNA using the Qiagen Oligotex mRNA mini kit (ref. 70022). Reverse transcription was performed with the Invitrogen SuperScript III first-strand synthesis kit (ref. 18080-051) using random hexamers for priming. PCR amplifications were performed using 0.2 ng of initial poly A+ RNA (as measured before doing the reverse transcription) in a final volume of 12.5 uL with JumpStart REDTaq ReadyMix (Sigma-Aldrich) and a primer concentration of 0.4 uM. Reactions took place in 384-well plates format on an automatized Evoware platform (TECAN) combined with a Tetrad2 thermocycler (Bio-Rad) that allows processing four plates in parallel. Because monoexonic amplification is sensitive to genomic DNA contaminations, monoexonic models were assessed by amplification of cDNA in which a dNTP analog was incorporated using the mRNA Selective PCR Kit (TAKARA). Illumina HiSeq 2000 Experimental Factor: organism part spleen LIBRARY_STRAND not applicable ERX682260 E-MTAB-3261:PolyA+_testis E-MTAB-3261:PolyA+_testis ERP009351 E-MTAB-3261 GENCODE PCR-Seq Batch M1 ERS643855 SAMEA3216849 E-MTAB-3261:PolyA+_testis PolyA+_testis AMPLICON TRANSCRIPTOMIC RT-PCR An 8-week-old C57/BL6 mouse purchased from Charles River was sacrificed, from which brain, heart, kidney, liver, lung, muscle (biceps femoris), spleen and testis were dissected. The tissues were homogenised in Trizol and total RNA was extracted. Any remaining DNA was removed with the Qiagen RNase-free DNase kit (ref. 79254) and total RNA was cleaned up with a Qiagen RNeasy mini kit (ref. 74104) column. Then, poly-A+ RNA was extracted from the total RNA using the Qiagen Oligotex mRNA mini kit (ref. 70022). Reverse transcription was performed with the Invitrogen SuperScript III first-strand synthesis kit (ref. 18080-051) using random hexamers for priming. PCR amplifications were performed using 0.2 ng of initial poly A+ RNA (as measured before doing the reverse transcription) in a final volume of 12.5 uL with JumpStart REDTaq ReadyMix (Sigma-Aldrich) and a primer concentration of 0.4 uM. Reactions took place in 384-well plates format on an automatized Evoware platform (TECAN) combined with a Tetrad2 thermocycler (Bio-Rad) that allows processing four plates in parallel. Because monoexonic amplification is sensitive to genomic DNA contaminations, monoexonic models were assessed by amplification of cDNA in which a dNTP analog was incorporated using the mRNA Selective PCR Kit (TAKARA). Illumina HiSeq 2000 Experimental Factor: organism part testis LIBRARY_STRAND not applicable ERX682261 E-MTAB-3261:PolyA+_heart E-MTAB-3261:PolyA+_heart ERP009351 E-MTAB-3261 GENCODE PCR-Seq Batch M1 ERS643856 SAMEA3216850 E-MTAB-3261:PolyA+_heart PolyA+_heart AMPLICON TRANSCRIPTOMIC RT-PCR An 8-week-old C57/BL6 mouse purchased from Charles River was sacrificed, from which brain, heart, kidney, liver, lung, muscle (biceps femoris), spleen and testis were dissected. The tissues were homogenised in Trizol and total RNA was extracted. Any remaining DNA was removed with the Qiagen RNase-free DNase kit (ref. 79254) and total RNA was cleaned up with a Qiagen RNeasy mini kit (ref. 74104) column. Then, poly-A+ RNA was extracted from the total RNA using the Qiagen Oligotex mRNA mini kit (ref. 70022). Reverse transcription was performed with the Invitrogen SuperScript III first-strand synthesis kit (ref. 18080-051) using random hexamers for priming. PCR amplifications were performed using 0.2 ng of initial poly A+ RNA (as measured before doing the reverse transcription) in a final volume of 12.5 uL with JumpStart REDTaq ReadyMix (Sigma-Aldrich) and a primer concentration of 0.4 uM. Reactions took place in 384-well plates format on an automatized Evoware platform (TECAN) combined with a Tetrad2 thermocycler (Bio-Rad) that allows processing four plates in parallel. Because monoexonic amplification is sensitive to genomic DNA contaminations, monoexonic models were assessed by amplification of cDNA in which a dNTP analog was incorporated using the mRNA Selective PCR Kit (TAKARA). Illumina HiSeq 2000 Experimental Factor: organism part heart LIBRARY_STRAND not applicable ERX682262 E-MTAB-3261:PolyA+_lung E-MTAB-3261:PolyA+_lung ERP009351 E-MTAB-3261 GENCODE PCR-Seq Batch M1 ERS643857 SAMEA3216851 E-MTAB-3261:PolyA+_lung PolyA+_lung AMPLICON TRANSCRIPTOMIC RT-PCR An 8-week-old C57/BL6 mouse purchased from Charles River was sacrificed, from which brain, heart, kidney, liver, lung, muscle (biceps femoris), spleen and testis were dissected. The tissues were homogenised in Trizol and total RNA was extracted. Any remaining DNA was removed with the Qiagen RNase-free DNase kit (ref. 79254) and total RNA was cleaned up with a Qiagen RNeasy mini kit (ref. 74104) column. Then, poly-A+ RNA was extracted from the total RNA using the Qiagen Oligotex mRNA mini kit (ref. 70022). Reverse transcription was performed with the Invitrogen SuperScript III first-strand synthesis kit (ref. 18080-051) using random hexamers for priming. PCR amplifications were performed using 0.2 ng of initial poly A+ RNA (as measured before doing the reverse transcription) in a final volume of 12.5 uL with JumpStart REDTaq ReadyMix (Sigma-Aldrich) and a primer concentration of 0.4 uM. Reactions took place in 384-well plates format on an automatized Evoware platform (TECAN) combined with a Tetrad2 thermocycler (Bio-Rad) that allows processing four plates in parallel. Because monoexonic amplification is sensitive to genomic DNA contaminations, monoexonic models were assessed by amplification of cDNA in which a dNTP analog was incorporated using the mRNA Selective PCR Kit (TAKARA). Illumina HiSeq 2000 Experimental Factor: organism part lung LIBRARY_STRAND not applicable ERX682263 E-MTAB-3261:PolyA+_muscle E-MTAB-3261:PolyA+_muscle ERP009351 E-MTAB-3261 GENCODE PCR-Seq Batch M1 ERS643858 SAMEA3216852 E-MTAB-3261:PolyA+_muscle PolyA+_muscle AMPLICON TRANSCRIPTOMIC RT-PCR An 8-week-old C57/BL6 mouse purchased from Charles River was sacrificed, from which brain, heart, kidney, liver, lung, muscle (biceps femoris), spleen and testis were dissected. The tissues were homogenised in Trizol and total RNA was extracted. Any remaining DNA was removed with the Qiagen RNase-free DNase kit (ref. 79254) and total RNA was cleaned up with a Qiagen RNeasy mini kit (ref. 74104) column. Then, poly-A+ RNA was extracted from the total RNA using the Qiagen Oligotex mRNA mini kit (ref. 70022). Reverse transcription was performed with the Invitrogen SuperScript III first-strand synthesis kit (ref. 18080-051) using random hexamers for priming. PCR amplifications were performed using 0.2 ng of initial poly A+ RNA (as measured before doing the reverse transcription) in a final volume of 12.5 uL with JumpStart REDTaq ReadyMix (Sigma-Aldrich) and a primer concentration of 0.4 uM. Reactions took place in 384-well plates format on an automatized Evoware platform (TECAN) combined with a Tetrad2 thermocycler (Bio-Rad) that allows processing four plates in parallel. Because monoexonic amplification is sensitive to genomic DNA contaminations, monoexonic models were assessed by amplification of cDNA in which a dNTP analog was incorporated using the mRNA Selective PCR Kit (TAKARA). Illumina HiSeq 2000 Experimental Factor: organism part skeletal muscle LIBRARY_STRAND not applicable