ERS687494 SAMEA3311345 4932 Saccharomyces cerevisiae Protocols: Grown in SCD-His media at 30oC to exponential phase (OD600 0.5-0.7), pelleted by centrifugation and snap frozen into liquid nitrogen. Samples lysed in Lola lysis buffer using a 6870 Freezer Mill (Spex). Lysates cleared by centrifugating twice at 15000 g. Nucleic acids extracted using Trizol Reagent. rRNA was depleted from samples using RibominusTM Eukaryote Kit for RNA-Seq (Invitrogen). Depleted samples were precipitated with 2.5x volumes ethanol, 1/10th volume 3M sodium acetate and 1μl glycogen, washed twice with 70% ethanol and re-suspended in 10 μl DEPC water. Sequencing libraries were generated using the whole Transcriptome Library preparation protocol provided with the SOLiD® Total RNA-Seq Kit. ENA-FIRST-PUBLIC 2016-03-31T17:36:25Z ENA-LAST-UPDATE 2018-03-08T23:56:54Z External Id SAMEA3311345 INSDC center name The University of Manchester INSDC first public 2016-03-31T17:36:25Z INSDC last update 2018-03-08T23:56:54Z INSDC status public Submitter Id E-MTAB-3407:JCSEQ141 broker name ArrayExpress common name baker's yeast genotype Mat a; his3 1; leu2 0; met15 0; ura3 0; YLL013c::kanMX4 sample name E-MTAB-3407:JCSEQ141 scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS687495 SAMEA3311346 4932 Saccharomyces cerevisiae Protocols: Grown in SCD-His media at 30oC to exponential phase (OD600 0.5-0.7), pelleted by centrifugation and snap frozen into liquid nitrogen. Samples lysed in Lola lysis buffer using a 6870 Freezer Mill (Spex). Lysates cleared by centrifugating twice at 15000 g. Nucleic acids extracted using Trizol Reagent. rRNA was depleted from samples using RibominusTM Eukaryote Kit for RNA-Seq (Invitrogen). Depleted samples were precipitated with 2.5x volumes ethanol, 1/10th volume 3M sodium acetate and 1μl glycogen, washed twice with 70% ethanol and re-suspended in 10 μl DEPC water. Sequencing libraries were generated using the whole Transcriptome Library preparation protocol provided with the SOLiD® Total RNA-Seq Kit. ENA-FIRST-PUBLIC 2016-03-31T17:36:25Z ENA-LAST-UPDATE 2018-03-08T23:56:54Z External Id SAMEA3311346 INSDC center name The University of Manchester INSDC first public 2016-03-31T17:36:25Z INSDC last update 2018-03-08T23:56:54Z INSDC status public Submitter Id E-MTAB-3407:JCSEQ139 broker name ArrayExpress common name baker's yeast genotype Mat a; his3 1; leu2 0; met15 0; ura3 0; YLL013c::kanMX4 sample name E-MTAB-3407:JCSEQ139 scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741 ERS687496 SAMEA3311347 4932 Saccharomyces cerevisiae Protocols: Grown in SCD-His media at 30oC to exponential phase (OD600 0.5-0.7), pelleted by centrifugation and snap frozen into liquid nitrogen. Samples lysed in Lola lysis buffer using a 6870 Freezer Mill (Spex). Lysates cleared by centrifugating twice at 15000 g. Nucleic acids extracted using Trizol Reagent. rRNA was depleted from samples using RibominusTM Eukaryote Kit for RNA-Seq (Invitrogen). Depleted samples were precipitated with 2.5x volumes ethanol, 1/10th volume 3M sodium acetate and 1μl glycogen, washed twice with 70% ethanol and re-suspended in 10 μl DEPC water. Sequencing libraries were generated using the whole Transcriptome Library preparation protocol provided with the SOLiD® Total RNA-Seq Kit. ENA-FIRST-PUBLIC 2016-03-31T17:36:25Z ENA-LAST-UPDATE 2018-03-08T23:56:54Z External Id SAMEA3311347 INSDC center name The University of Manchester INSDC first public 2016-03-31T17:36:25Z INSDC last update 2018-03-08T23:56:54Z INSDC status public Submitter Id E-MTAB-3407:JCSEQ137 broker name ArrayExpress common name baker's yeast genotype Mat a; his3 1; leu2 0; met15 0; ura3 0; YLL013c::kanMX4 sample name E-MTAB-3407:JCSEQ137 scientific_name Saccharomyces cerevisiae sex Mat a specimen with known storage state fresh specimen strain BY4741