ERS710488 SAMEA3356196 10090 Mus musculus Protocols: Isolated neural stem cells from E18.5 or adult Swiss Webster mice were grown under non-adherent conditions for three weeks/two passages (eNSCs) or four weeks/three passages (aNSCs) prior to RNA isolation Embryonic NSCs were acutely transduced with empty vector (EV) or Bmi1-overexpressing lentiviral vector; adult NSCs were allowed to recover overnight prior to transduction Total RNA was isolated using RNAeasy Plus Micro kit (Qiagen) 150-250 ng RNA was used for library construction. Ribosomal RNA was removed using the Ribo-Zero Magnetic Kit (Epicentre), and libraries prepared using the ScripSeq V2 RNA-seq Kit (Epicentre), using the low input protocol. ENA-FIRST-PUBLIC 2015-12-01T17:02:04Z ENA-LAST-UPDATE 2018-03-09T00:37:20Z External Id SAMEA3356196 INSDC center name Wadsworth Center, NY State Dept of Health INSDC first public 2015-12-01T17:02:04Z INSDC last update 2018-03-09T00:37:20Z INSDC status public Submitter Id E-MTAB-3491:eNSC Bmi1-2 broker name ArrayExpress cell type neural stem cell common name house mouse developmental stage embryonic day 18.5 genetic modification Bmi1-overexpressing lentiviral vector growth condition neurospheres sample name E-MTAB-3491:eNSC Bmi1-2 scientific_name Mus musculus ERS710489 SAMEA3356197 10090 Mus musculus Protocols: Isolated neural stem cells from E18.5 or adult Swiss Webster mice were grown under non-adherent conditions for three weeks/two passages (eNSCs) or four weeks/three passages (aNSCs) prior to RNA isolation Embryonic NSCs were acutely transduced with empty vector (EV) or Bmi1-overexpressing lentiviral vector; adult NSCs were allowed to recover overnight prior to transduction Total RNA was isolated using RNAeasy Plus Micro kit (Qiagen) 150-250 ng RNA was used for library construction. Ribosomal RNA was removed using the Ribo-Zero Magnetic Kit (Epicentre), and libraries prepared using the ScripSeq V2 RNA-seq Kit (Epicentre), using the low input protocol. ENA-FIRST-PUBLIC 2015-12-01T17:02:04Z ENA-LAST-UPDATE 2018-03-09T00:37:20Z External Id SAMEA3356197 INSDC center name Wadsworth Center, NY State Dept of Health INSDC first public 2015-12-01T17:02:04Z INSDC last update 2018-03-09T00:37:20Z INSDC status public Submitter Id E-MTAB-3491:aNSC EV-2 broker name ArrayExpress cell type neural stem cell common name house mouse developmental stage adult genetic modification empty vector control growth condition neurospheres sample name E-MTAB-3491:aNSC EV-2 scientific_name Mus musculus ERS710490 SAMEA3356198 10090 Mus musculus Protocols: Isolated neural stem cells from E18.5 or adult Swiss Webster mice were grown under non-adherent conditions for three weeks/two passages (eNSCs) or four weeks/three passages (aNSCs) prior to RNA isolation Embryonic NSCs were acutely transduced with empty vector (EV) or Bmi1-overexpressing lentiviral vector; adult NSCs were allowed to recover overnight prior to transduction Total RNA was isolated using RNAeasy Plus Micro kit (Qiagen) 150-250 ng RNA was used for library construction. Ribosomal RNA was removed using the Ribo-Zero Magnetic Kit (Epicentre), and libraries prepared using the ScripSeq V2 RNA-seq Kit (Epicentre), using the low input protocol. ENA-FIRST-PUBLIC 2015-12-01T17:02:04Z ENA-LAST-UPDATE 2018-03-09T00:37:20Z External Id SAMEA3356198 INSDC center name Wadsworth Center, NY State Dept of Health INSDC first public 2015-12-01T17:02:04Z INSDC last update 2018-03-09T00:37:20Z INSDC status public Submitter Id E-MTAB-3491:eNSC H1-2 broker name ArrayExpress cell type neural stem cell common name house mouse developmental stage embryonic day 18.5 genetic modification empty vector control growth condition neurospheres sample name E-MTAB-3491:eNSC H1-2 scientific_name Mus musculus ERS710491 SAMEA3356199 10090 Mus musculus Protocols: Isolated neural stem cells from E18.5 or adult Swiss Webster mice were grown under non-adherent conditions for three weeks/two passages (eNSCs) or four weeks/three passages (aNSCs) prior to RNA isolation Embryonic NSCs were acutely transduced with empty vector (EV) or Bmi1-overexpressing lentiviral vector; adult NSCs were allowed to recover overnight prior to transduction Total RNA was isolated using RNAeasy Plus Micro kit (Qiagen) 150-250 ng RNA was used for library construction. Ribosomal RNA was removed using the Ribo-Zero Magnetic Kit (Epicentre), and libraries prepared using the ScripSeq V2 RNA-seq Kit (Epicentre), using the low input protocol. ENA-FIRST-PUBLIC 2015-12-01T17:02:04Z ENA-LAST-UPDATE 2018-03-09T00:37:20Z External Id SAMEA3356199 INSDC center name Wadsworth Center, NY State Dept of Health INSDC first public 2015-12-01T17:02:04Z INSDC last update 2018-03-09T00:37:20Z INSDC status public Submitter Id E-MTAB-3491:aNSC Bmi1-1 broker name ArrayExpress cell type neural stem cell common name house mouse developmental stage adult genetic modification Bmi1-overexpressing lentiviral vector growth condition neurospheres sample name E-MTAB-3491:aNSC Bmi1-1 scientific_name Mus musculus ERS710492 SAMEA3356200 10090 Mus musculus Protocols: Isolated neural stem cells from E18.5 or adult Swiss Webster mice were grown under non-adherent conditions for three weeks/two passages (eNSCs) or four weeks/three passages (aNSCs) prior to RNA isolation Embryonic NSCs were acutely transduced with empty vector (EV) or Bmi1-overexpressing lentiviral vector; adult NSCs were allowed to recover overnight prior to transduction Total RNA was isolated using RNAeasy Plus Micro kit (Qiagen) 150-250 ng RNA was used for library construction. Ribosomal RNA was removed using the Ribo-Zero Magnetic Kit (Epicentre), and libraries prepared using the ScripSeq V2 RNA-seq Kit (Epicentre), using the low input protocol. ENA-FIRST-PUBLIC 2015-12-01T17:02:04Z ENA-LAST-UPDATE 2018-03-09T00:37:36Z External Id SAMEA3356200 INSDC center name Wadsworth Center, NY State Dept of Health INSDC first public 2015-12-01T17:02:04Z INSDC last update 2018-03-09T00:37:36Z INSDC status public Submitter Id E-MTAB-3491:aNSC EV-1 broker name ArrayExpress cell type neural stem cell common name house mouse developmental stage adult genetic modification empty vector control growth condition neurospheres sample name E-MTAB-3491:aNSC EV-1 scientific_name Mus musculus ERS710493 SAMEA3356201 10090 Mus musculus Protocols: Isolated neural stem cells from E18.5 or adult Swiss Webster mice were grown under non-adherent conditions for three weeks/two passages (eNSCs) or four weeks/three passages (aNSCs) prior to RNA isolation Embryonic NSCs were acutely transduced with empty vector (EV) or Bmi1-overexpressing lentiviral vector; adult NSCs were allowed to recover overnight prior to transduction Total RNA was isolated using RNAeasy Plus Micro kit (Qiagen) 150-250 ng RNA was used for library construction. Ribosomal RNA was removed using the Ribo-Zero Magnetic Kit (Epicentre), and libraries prepared using the ScripSeq V2 RNA-seq Kit (Epicentre), using the low input protocol. ENA-FIRST-PUBLIC 2015-12-01T17:02:04Z ENA-LAST-UPDATE 2018-03-09T00:37:36Z External Id SAMEA3356201 INSDC center name Wadsworth Center, NY State Dept of Health INSDC first public 2015-12-01T17:02:04Z INSDC last update 2018-03-09T00:37:36Z INSDC status public Submitter Id E-MTAB-3491:aNSC Bmi1-2 broker name ArrayExpress cell type neural stem cell common name house mouse developmental stage adult genetic modification Bmi1-overexpressing lentiviral vector growth condition neurospheres sample name E-MTAB-3491:aNSC Bmi1-2 scientific_name Mus musculus ERS710494 SAMEA3356202 10090 Mus musculus Protocols: Isolated neural stem cells from E18.5 or adult Swiss Webster mice were grown under non-adherent conditions for three weeks/two passages (eNSCs) or four weeks/three passages (aNSCs) prior to RNA isolation Embryonic NSCs were acutely transduced with empty vector (EV) or Bmi1-overexpressing lentiviral vector; adult NSCs were allowed to recover overnight prior to transduction Total RNA was isolated using RNAeasy Plus Micro kit (Qiagen) 150-250 ng RNA was used for library construction. Ribosomal RNA was removed using the Ribo-Zero Magnetic Kit (Epicentre), and libraries prepared using the ScripSeq V2 RNA-seq Kit (Epicentre), using the low input protocol. ENA-FIRST-PUBLIC 2015-12-01T17:02:04Z ENA-LAST-UPDATE 2018-03-09T00:37:36Z External Id SAMEA3356202 INSDC center name Wadsworth Center, NY State Dept of Health INSDC first public 2015-12-01T17:02:04Z INSDC last update 2018-03-09T00:37:36Z INSDC status public Submitter Id E-MTAB-3491:eNSC H1-1 broker name ArrayExpress cell type neural stem cell common name house mouse developmental stage embryonic day 18.5 genetic modification empty vector control growth condition neurospheres sample name E-MTAB-3491:eNSC H1-1 scientific_name Mus musculus ERS710495 SAMEA3356203 10090 Mus musculus Protocols: Isolated neural stem cells from E18.5 or adult Swiss Webster mice were grown under non-adherent conditions for three weeks/two passages (eNSCs) or four weeks/three passages (aNSCs) prior to RNA isolation Embryonic NSCs were acutely transduced with empty vector (EV) or Bmi1-overexpressing lentiviral vector; adult NSCs were allowed to recover overnight prior to transduction Total RNA was isolated using RNAeasy Plus Micro kit (Qiagen) 150-250 ng RNA was used for library construction. Ribosomal RNA was removed using the Ribo-Zero Magnetic Kit (Epicentre), and libraries prepared using the ScripSeq V2 RNA-seq Kit (Epicentre), using the low input protocol. ENA-FIRST-PUBLIC 2015-12-01T17:02:04Z ENA-LAST-UPDATE 2018-03-09T00:37:36Z External Id SAMEA3356203 INSDC center name Wadsworth Center, NY State Dept of Health INSDC first public 2015-12-01T17:02:04Z INSDC last update 2018-03-09T00:37:36Z INSDC status public Submitter Id E-MTAB-3491:eNSC Bmi1-1 broker name ArrayExpress cell type neural stem cell common name house mouse developmental stage embryonic day 18.5 genetic modification Bmi1-overexpressing lentiviral vector growth condition neurospheres sample name E-MTAB-3491:eNSC Bmi1-1 scientific_name Mus musculus