ERP010232 PRJEB9153 E-MTAB-3491 E-MTAB-3491 Control and Bmi1-overexpressing adult murine neural stem cells Neural stem cells were isolated from embryonic (E18) cortex and from adult mouse subventricular zone and transduced with a Bmi1-overexpressing lentiviral vector or an empty vector control. Cells were grown as neurospheres (in non-adherent culture conditions) for two passages for eNSCs and three passages for aNSCs and RNA purified (after three weeks for eNSCs and four weeks for aNSCs). Control and Bmi1-overexpressing adult murine neural stem cells Neural stem cells were isolated from embryonic (E18) cortex and from adult mouse subventricular zone and transduced with a Bmi1-overexpressing lentiviral vector or an empty vector control. Cells were grown as neurospheres (in non-adherent culture conditions) for two passages for eNSCs and three passages for aNSCs and RNA purified (after three weeks for eNSCs and four weeks for aNSCs). E-MTAB-3491 in ArrayExpress http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-3491 ArrayExpress E-MTAB-3491