ERX1030597 E-MTAB-3695:ZJ1_H3K18ac E-MTAB-3695:ZJ1_H3K18ac ERP010910 E-MTAB-3695 Histone H3K18ac ChIP-seq in U2OS cells ERS784131 SAMEA3472022 E-MTAB-3695:ZJ1_H3K18ac ZJ1_H3K18ac ChIP-Seq GENOMIC ChIP 'The human osteosarcoma U2OS cells were grown in DMEM supplement with 10% foetal bovine serum for 24h, then cells were transfected for 48 hours with ON-TARGETplus Non-targeting Pool (Dharmacon D-001810-10) using Lipofectamine RNAiMax (Life technologies) according to manufacturer''s protocol. Cells were cross-linked with 1% Formaldehyde for 10 minutes.' After reverse cross-link and protein digestion with Proteinase K (Roche), DNA was purified using Qiaquick, PCR purification kit (Qiagen) following the manufacturer’s instructions. ChIP-seq was performed as described in Schmidt et al., 2009, using 10 x 106 U2OS. Immuno-precipitation was performed overnight at 4° C by incubating the shared DNA-protein complex with 10 μg of anti-H3K18Ac (Abcam AB1191 ) antibodies previously coupled to Dynabeads protein G (Invitrogen). Schmidt D, Wilson MD, Spyrou C, Brown GD, Hadfield J, Odom DT. (2009) ChIP-seq: using high-throughput sequencing to discover protein-DNA interactions. Methods 48: 240–248. Illumina Genome Analyzer IIx Experimental Factor: anti-H3K18ac immunoprecipitate ERX1030598 E-MTAB-3695:ZJ3_input E-MTAB-3695:ZJ3_input ERP010910 E-MTAB-3695 Histone H3K18ac ChIP-seq in U2OS cells ERS784132 SAMEA3472023 E-MTAB-3695:ZJ3_input ZJ3_input ChIP-Seq GENOMIC RANDOM 'The human osteosarcoma U2OS cells were grown in DMEM supplement with 10% foetal bovine serum for 24h, then cells were transfected for 48 hours with ON-TARGETplus Non-targeting Pool (Dharmacon D-001810-10) using Lipofectamine RNAiMax (Life technologies) according to manufacturer''s protocol. Cells were cross-linked with 1% Formaldehyde for 10 minutes.' After reverse cross-link and protein digestion with Proteinase K (Roche), DNA was purified using Qiaquick, PCR purification kit (Qiagen) following the manufacturer’s instructions. ChIP-seq was performed as described in Schmidt et al., 2009, using 10 x 106 U2OS. Immuno-precipitation was performed overnight at 4° C by incubating the shared DNA-protein complex with 10 μg of anti-H3K18Ac (Abcam AB1191 ) antibodies previously coupled to Dynabeads protein G (Invitrogen). Schmidt D, Wilson MD, Spyrou C, Brown GD, Hadfield J, Odom DT. (2009) ChIP-seq: using high-throughput sequencing to discover protein-DNA interactions. Methods 48: 240–248. Illumina Genome Analyzer IIx Experimental Factor: input DNA immunoprecipitate