ERX1092055 E-MTAB-3868:CB11130_A E-MTAB-3868:CB11130_A ERP012006 E-MTAB-3868 ATAC-seq of BMP response in glioblastoma-derived neural stem cells ERS839181 SAMEA3532032 E-MTAB-3868:CB11130_A CB11130_A OTHER GENOMIC PCR GNS and NS cells were cultured using serum-free basal media supplemented with B27 and N2 culture supplements (Life Technologies). Growth factors EGF and FGF-2 (20 ng/ml) were added. Culture vessels were coated with Laminin (Sigma) for 3 hr at 10 ug/ml prior to use. Alternatively media was directly supplemented with 1 ug/ml Laminin. GNS cells were routinely grown to confluence, dissociated using Accutase (Sigma), and then split 1:3 to 1:7. Medium was replaced every 3-7 days. GNS and NS cells were split as appropriate to maintain a plating density of ~10 cells/mm2; differentiating cells were maintained in media supplemented with bone morphogenic protein (BMP) without EGF and FGF-2. After 8 days’ treatment cells were harvested, counted and pelleted in aliquots of 50,000 cells. Cells were lysed and the nuclei were pelleted then suspended in buffer and treated with Tn5 transposase for 30 min. ATAC library preparation was undertaken according to published protocols (Buenrostro et al. 2013, Nat Methods 10:1213-8). DNA was purified using Qiagen MinElute columns, followed by 12 cycles of PCR amplification using NEBNext High Fidelity Master Mix and custom primers, with adapter sequences previously specified for ATAC-seq (Buenrostro et al., 2013), followed by further purification on MinElute columns. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 ERX1092056 E-MTAB-3868:CB11130_B E-MTAB-3868:CB11130_B ERP012006 E-MTAB-3868 ATAC-seq of BMP response in glioblastoma-derived neural stem cells ERS839182 SAMEA3532033 E-MTAB-3868:CB11130_B CB11130_B OTHER GENOMIC PCR GNS and NS cells were cultured using serum-free basal media supplemented with B27 and N2 culture supplements (Life Technologies). Growth factors EGF and FGF-2 (20 ng/ml) were added. Culture vessels were coated with Laminin (Sigma) for 3 hr at 10 ug/ml prior to use. Alternatively media was directly supplemented with 1 ug/ml Laminin. GNS cells were routinely grown to confluence, dissociated using Accutase (Sigma), and then split 1:3 to 1:7. Medium was replaced every 3-7 days. GNS and NS cells were split as appropriate to maintain a plating density of ~10 cells/mm2; differentiating cells were maintained in media supplemented with bone morphogenic protein (BMP) without EGF and FGF-2. After 8 days’ treatment cells were harvested, counted and pelleted in aliquots of 50,000 cells. Cells were lysed and the nuclei were pelleted then suspended in buffer and treated with Tn5 transposase for 30 min. ATAC library preparation was undertaken according to published protocols (Buenrostro et al. 2013, Nat Methods 10:1213-8). DNA was purified using Qiagen MinElute columns, followed by 12 cycles of PCR amplification using NEBNext High Fidelity Master Mix and custom primers, with adapter sequences previously specified for ATAC-seq (Buenrostro et al., 2013), followed by further purification on MinElute columns. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 ERX1092057 E-MTAB-3868:CB11130_BMP_A E-MTAB-3868:CB11130_BMP_A ERP012006 E-MTAB-3868 ATAC-seq of BMP response in glioblastoma-derived neural stem cells ERS839183 SAMEA3532034 E-MTAB-3868:CB11130_BMP_A CB11130_BMP_A OTHER GENOMIC PCR GNS and NS cells were cultured using serum-free basal media supplemented with B27 and N2 culture supplements (Life Technologies). Growth factors EGF and FGF-2 (20 ng/ml) were added. Culture vessels were coated with Laminin (Sigma) for 3 hr at 10 ug/ml prior to use. Alternatively media was directly supplemented with 1 ug/ml Laminin. GNS cells were routinely grown to confluence, dissociated using Accutase (Sigma), and then split 1:3 to 1:7. Medium was replaced every 3-7 days. GNS and NS cells were split as appropriate to maintain a plating density of ~10 cells/mm2; differentiating cells were maintained in media supplemented with bone morphogenic protein (BMP) without EGF and FGF-2. After 8 days’ treatment cells were harvested, counted and pelleted in aliquots of 50,000 cells. Cells were lysed and the nuclei were pelleted then suspended in buffer and treated with Tn5 transposase for 30 min. ATAC library preparation was undertaken according to published protocols (Buenrostro et al. 2013, Nat Methods 10:1213-8). DNA was purified using Qiagen MinElute columns, followed by 12 cycles of PCR amplification using NEBNext High Fidelity Master Mix and custom primers, with adapter sequences previously specified for ATAC-seq (Buenrostro et al., 2013), followed by further purification on MinElute columns. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: bone morphogenic protein stimulus ERX1092058 E-MTAB-3868:CB11130_BMP_B E-MTAB-3868:CB11130_BMP_B ERP012006 E-MTAB-3868 ATAC-seq of BMP response in glioblastoma-derived neural stem cells ERS839184 SAMEA3532035 E-MTAB-3868:CB11130_BMP_B CB11130_BMP_B OTHER GENOMIC PCR GNS and NS cells were cultured using serum-free basal media supplemented with B27 and N2 culture supplements (Life Technologies). Growth factors EGF and FGF-2 (20 ng/ml) were added. Culture vessels were coated with Laminin (Sigma) for 3 hr at 10 ug/ml prior to use. Alternatively media was directly supplemented with 1 ug/ml Laminin. GNS cells were routinely grown to confluence, dissociated using Accutase (Sigma), and then split 1:3 to 1:7. Medium was replaced every 3-7 days. GNS and NS cells were split as appropriate to maintain a plating density of ~10 cells/mm2; differentiating cells were maintained in media supplemented with bone morphogenic protein (BMP) without EGF and FGF-2. After 8 days’ treatment cells were harvested, counted and pelleted in aliquots of 50,000 cells. Cells were lysed and the nuclei were pelleted then suspended in buffer and treated with Tn5 transposase for 30 min. ATAC library preparation was undertaken according to published protocols (Buenrostro et al. 2013, Nat Methods 10:1213-8). DNA was purified using Qiagen MinElute columns, followed by 12 cycles of PCR amplification using NEBNext High Fidelity Master Mix and custom primers, with adapter sequences previously specified for ATAC-seq (Buenrostro et al., 2013), followed by further purification on MinElute columns. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: bone morphogenic protein stimulus ERX1092059 E-MTAB-3868:G26_A E-MTAB-3868:G26_A ERP012006 E-MTAB-3868 ATAC-seq of BMP response in glioblastoma-derived neural stem cells ERS839185 SAMEA3532036 E-MTAB-3868:G26_A G26_A OTHER GENOMIC PCR GNS and NS cells were cultured using serum-free basal media supplemented with B27 and N2 culture supplements (Life Technologies). Growth factors EGF and FGF-2 (20 ng/ml) were added. Culture vessels were coated with Laminin (Sigma) for 3 hr at 10 ug/ml prior to use. Alternatively media was directly supplemented with 1 ug/ml Laminin. GNS cells were routinely grown to confluence, dissociated using Accutase (Sigma), and then split 1:3 to 1:7. Medium was replaced every 3-7 days. GNS and NS cells were split as appropriate to maintain a plating density of ~10 cells/mm2; differentiating cells were maintained in media supplemented with bone morphogenic protein (BMP) without EGF and FGF-2. After 8 days’ treatment cells were harvested, counted and pelleted in aliquots of 50,000 cells. Cells were lysed and the nuclei were pelleted then suspended in buffer and treated with Tn5 transposase for 30 min. ATAC library preparation was undertaken according to published protocols (Buenrostro et al. 2013, Nat Methods 10:1213-8). DNA was purified using Qiagen MinElute columns, followed by 12 cycles of PCR amplification using NEBNext High Fidelity Master Mix and custom primers, with adapter sequences previously specified for ATAC-seq (Buenrostro et al., 2013), followed by further purification on MinElute columns. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: glioblastoma multiforme disease ERX1092060 E-MTAB-3868:G26_B E-MTAB-3868:G26_B ERP012006 E-MTAB-3868 ATAC-seq of BMP response in glioblastoma-derived neural stem cells ERS839186 SAMEA3532037 E-MTAB-3868:G26_B G26_B OTHER GENOMIC PCR GNS and NS cells were cultured using serum-free basal media supplemented with B27 and N2 culture supplements (Life Technologies). Growth factors EGF and FGF-2 (20 ng/ml) were added. Culture vessels were coated with Laminin (Sigma) for 3 hr at 10 ug/ml prior to use. Alternatively media was directly supplemented with 1 ug/ml Laminin. GNS cells were routinely grown to confluence, dissociated using Accutase (Sigma), and then split 1:3 to 1:7. Medium was replaced every 3-7 days. GNS and NS cells were split as appropriate to maintain a plating density of ~10 cells/mm2; differentiating cells were maintained in media supplemented with bone morphogenic protein (BMP) without EGF and FGF-2. After 8 days’ treatment cells were harvested, counted and pelleted in aliquots of 50,000 cells. Cells were lysed and the nuclei were pelleted then suspended in buffer and treated with Tn5 transposase for 30 min. ATAC library preparation was undertaken according to published protocols (Buenrostro et al. 2013, Nat Methods 10:1213-8). DNA was purified using Qiagen MinElute columns, followed by 12 cycles of PCR amplification using NEBNext High Fidelity Master Mix and custom primers, with adapter sequences previously specified for ATAC-seq (Buenrostro et al., 2013), followed by further purification on MinElute columns. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: glioblastoma multiforme disease ERX1092061 E-MTAB-3868:G26_BMP_A E-MTAB-3868:G26_BMP_A ERP012006 E-MTAB-3868 ATAC-seq of BMP response in glioblastoma-derived neural stem cells ERS839187 SAMEA3532038 E-MTAB-3868:G26_BMP_A G26_BMP_A OTHER GENOMIC PCR GNS and NS cells were cultured using serum-free basal media supplemented with B27 and N2 culture supplements (Life Technologies). Growth factors EGF and FGF-2 (20 ng/ml) were added. Culture vessels were coated with Laminin (Sigma) for 3 hr at 10 ug/ml prior to use. Alternatively media was directly supplemented with 1 ug/ml Laminin. GNS cells were routinely grown to confluence, dissociated using Accutase (Sigma), and then split 1:3 to 1:7. Medium was replaced every 3-7 days. GNS and NS cells were split as appropriate to maintain a plating density of ~10 cells/mm2; differentiating cells were maintained in media supplemented with bone morphogenic protein (BMP) without EGF and FGF-2. After 8 days’ treatment cells were harvested, counted and pelleted in aliquots of 50,000 cells. Cells were lysed and the nuclei were pelleted then suspended in buffer and treated with Tn5 transposase for 30 min. ATAC library preparation was undertaken according to published protocols (Buenrostro et al. 2013, Nat Methods 10:1213-8). DNA was purified using Qiagen MinElute columns, followed by 12 cycles of PCR amplification using NEBNext High Fidelity Master Mix and custom primers, with adapter sequences previously specified for ATAC-seq (Buenrostro et al., 2013), followed by further purification on MinElute columns. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: glioblastoma multiforme disease Experimental Factor: bone morphogenic protein stimulus ERX1092062 E-MTAB-3868:G26_BMP_B E-MTAB-3868:G26_BMP_B ERP012006 E-MTAB-3868 ATAC-seq of BMP response in glioblastoma-derived neural stem cells ERS839188 SAMEA3532039 E-MTAB-3868:G26_BMP_B G26_BMP_B OTHER GENOMIC PCR GNS and NS cells were cultured using serum-free basal media supplemented with B27 and N2 culture supplements (Life Technologies). Growth factors EGF and FGF-2 (20 ng/ml) were added. Culture vessels were coated with Laminin (Sigma) for 3 hr at 10 ug/ml prior to use. Alternatively media was directly supplemented with 1 ug/ml Laminin. GNS cells were routinely grown to confluence, dissociated using Accutase (Sigma), and then split 1:3 to 1:7. Medium was replaced every 3-7 days. GNS and NS cells were split as appropriate to maintain a plating density of ~10 cells/mm2; differentiating cells were maintained in media supplemented with bone morphogenic protein (BMP) without EGF and FGF-2. After 8 days’ treatment cells were harvested, counted and pelleted in aliquots of 50,000 cells. Cells were lysed and the nuclei were pelleted then suspended in buffer and treated with Tn5 transposase for 30 min. ATAC library preparation was undertaken according to published protocols (Buenrostro et al. 2013, Nat Methods 10:1213-8). DNA was purified using Qiagen MinElute columns, followed by 12 cycles of PCR amplification using NEBNext High Fidelity Master Mix and custom primers, with adapter sequences previously specified for ATAC-seq (Buenrostro et al., 2013), followed by further purification on MinElute columns. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: glioblastoma multiforme disease Experimental Factor: bone morphogenic protein stimulus