ERS7094944 SAMEA9361877 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361877 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:170921_d29_409b2 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition continuous exposure to doxycycline phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:170921_d29_409b2 ERS7094945 SAMEA9361880 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361880 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:171122_d1 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition continuous exposure to doxycycline phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:171122_d1 ERS7094946 SAMEA9361879 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361879 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:171122_d28 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition continuous exposure to doxycycline phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:171122_d28 ERS7094947 SAMEA9361881 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361881 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:171122_h0 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition continuous exposure to doxycycline phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:171122_h0 ERS7094948 SAMEA9361878 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361878 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:171212_d14 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition continuous exposure to doxycycline phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:171212_d14 ERS7094949 SAMEA9361882 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361882 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:171212_d5 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition continuous exposure to doxycycline phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:171212_d5 ERS7094950 SAMEA9361884 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361884 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:180221_d35 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition continuous exposure to doxycycline phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:180221_d35 ERS7094951 SAMEA9361883 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361883 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:180321_d28 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition continuous exposure to doxycycline phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:180321_d28 ERS7094952 SAMEA9361885 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361885 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:180410_d35 broker name ArrayExpress cell line 409b2_monoclonal/Sc102a1 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition continuous exposure to doxycycline phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:180410_d35 ERS7094953 SAMEA9361886 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361886 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:180420_d2 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition continuous exposure to doxycycline phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:180420_d2 ERS7094954 SAMEA9361887 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361887 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:180628_h6/12 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition continuous exposure to doxycycline phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:180628_h6/12 ERS7094955 SAMEA9361888 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361888 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:180904_d5 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition continuous exposure to doxycycline phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:180904_d5 ERS7094956 SAMEA9361889 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361889 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:iN_Dox_ctrl broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition continuous exposure to doxycycline phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:iN_Dox_ctrl ERS7094957 SAMEA9361890 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361890 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:iN_Dox_d1 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition doxycycline removed after 1 day phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:iN_Dox_d1 ERS7094958 SAMEA9361891 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361891 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:iN_Dox_d3 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition doxycycline removed after 3 days phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:iN_Dox_d3 ERS7094959 SAMEA9361892 9606 Homo sapiens Protocols: Ngn2-iNs were selectively dissociated from co-cultured rat astrocytes using a mild dissociation procedure. Enriched neural cell suspensions were diluted to 500-1000 cells/mL. Single cells were encapsulated along with beads and reverse transcription reagents into droplets using 10X microfluidic chip system. Reverse transcription of first strand cDNA was performed in droplets. Induced pluripotent stem cells and corresponding rtTA/Ngn2-derivatives were cultured in standard feeder-free conditions in mTeSR1 (StemCell Technologies) on plates coated with matrigel (Corning). Primary cortical rat astrocytes (Gibco) were cultured in high-glucose DMEM containing 10% FCS and 1% Pen/Strep on plates coated with poly-D-Lysine (Sigma-Aldrich). Fresh media was added to the astrocytes every 4-5 days and passaged once a week with Trypsin-EDTA digestion at a standard ratio of 1:2. Astrocytes were used up to passage 10, with passage 0 being the culture of initial isolation. rtTA/Ngn2 double positive stem cell lines were generated and differentiated into Ngn2-iNs as previously described (Frega et al., 2017). Single stranded cDNA was cleaned up and amplified after breaking the droplets generated during the sample collection using the 10X 3' Single cell Kit v2 according to the manufacturer's instructions. Quality and quantity of the resulting cDNA was assessed using Agilent's Bioanalyzer High Sensitivity DNA assay. Libraries were constructed following the 10X library construction protocol v2. Briefly, cDNA was fragmented, end repaired and adaptor sequences were ligated, followed by a PCR to itroduce library specific sequencing primers as provided by 10X. Libraries were quantified and checked for size distribution using Agilent Bioanalyzer DNA High Sensitivity Assays. ENA first public 2021-08-18 ENA last update 2021-08-18 External Id SAMEA9361892 INSDC center alias D-BSSE, ETH ZURICH INSDC center name D-BSSE, ETH ZURICH INSDC first public 2021-08-18T12:20:02Z INSDC last update 2021-08-18T12:20:02Z INSDC status public Submitter Id E-MTAB-10632:iN_Dox_d5 broker name ArrayExpress cell line 409b2 cell type Ngn2-induced neuron common name human genotype rtTA-Ngn2 growth condition doxycycline removed after 5 days phenotype doxycycline induced overexpression of Neurogenin2 progenitor cell type induced pluripotent stem cell sample name E-MTAB-10632:iN_Dox_d5