ERS7121543 SAMEA9405865 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405865 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 1 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype wild type genotype organism part bone marrow sample name E-MTAB-10732:Sample 1 sex female strain C57BL/6 ERS7121544 SAMEA9405867 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405867 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 10 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype CD40LG -/- organism part bone marrow sample name E-MTAB-10732:Sample 10 sex female strain C57BL/6 ERS7121545 SAMEA9405866 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405866 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 11 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype CD40LG -/- organism part bone marrow sample name E-MTAB-10732:Sample 11 sex female strain C57BL/6 ERS7121546 SAMEA9405868 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405868 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 12 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype CD40LG -/- organism part bone marrow sample name E-MTAB-10732:Sample 12 sex female strain C57BL/6 ERS7121547 SAMEA9405869 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405869 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 13 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype CD40LG -/- organism part bone marrow sample name E-MTAB-10732:Sample 13 sex female strain C57BL/6 ERS7121548 SAMEA9405870 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405870 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 14 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype CD40LG -/- organism part bone marrow sample name E-MTAB-10732:Sample 14 sex female strain C57BL/6 ERS7121549 SAMEA9405871 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405871 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 15 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype CD40LG -/- organism part bone marrow sample name E-MTAB-10732:Sample 15 sex female strain C57BL/6 ERS7121550 SAMEA9405872 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405872 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 16 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype CD40LG -/- organism part bone marrow sample name E-MTAB-10732:Sample 16 sex female strain C57BL/6 ERS7121551 SAMEA9405873 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405873 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 17 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype CD40LG -/- organism part bone marrow sample name E-MTAB-10732:Sample 17 sex female strain C57BL/6 ERS7121552 SAMEA9405874 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405874 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 18 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype CD40LG -/- organism part bone marrow sample name E-MTAB-10732:Sample 18 sex female strain C57BL/6 ERS7121553 SAMEA9405875 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405875 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 2 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype wild type genotype organism part bone marrow sample name E-MTAB-10732:Sample 2 sex female strain C57BL/6 ERS7121554 SAMEA9405876 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405876 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 3 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype wild type genotype organism part bone marrow sample name E-MTAB-10732:Sample 3 sex female strain C57BL/6 ERS7121555 SAMEA9405877 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405877 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 4 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype wild type genotype organism part bone marrow sample name E-MTAB-10732:Sample 4 sex female strain C57BL/6 ERS7121556 SAMEA9405878 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405878 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 5 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype wild type genotype organism part bone marrow sample name E-MTAB-10732:Sample 5 sex female strain C57BL/6 ERS7121557 SAMEA9405879 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405879 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 6 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype wild type genotype organism part bone marrow sample name E-MTAB-10732:Sample 6 sex female strain C57BL/6 ERS7121558 SAMEA9405880 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405880 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 7 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype wild type genotype organism part bone marrow sample name E-MTAB-10732:Sample 7 sex female strain C57BL/6 ERS7121559 SAMEA9405881 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405881 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 8 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype wild type genotype organism part bone marrow sample name E-MTAB-10732:Sample 8 sex female strain C57BL/6 ERS7121560 SAMEA9405882 10090 Mus musculus Protocols: Bone marrow cells were harvested aseptically from both femur and tibia bones and processed immediately for analysis. Mouse bone marrow neutrophils were purified by negative selection on an autoMACS cell separator using a neutrophil isolation kit (Miltenyi Biotec, Cologne, BG) and following the manufacturer's instructions. The cell purity obtained using this procedure was verified by FACS staining of purified cells using antibodies specific to CD11b and Ly6G and was routinely found to be 90-95%. After purification, RNA from bone marrow neutrophils was isolated by TRIZOL reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. When necessary, cells obtained from two different mice of the same group and processed simultaneously were pooled to obtain the number of cells required to perform the sequencing. RNA integrity and concentration were assessed using the Agilent 2100 Bioanalyser RNA Nanochip. Samples with RNA integrity number (RIN) ≥ 8 were used for thr transcriptome analysis. cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ was performed using the NEBNext Ultra Sample Preparation Kit (Illumina Inc, San Diego, CA) according to each manufacturer's instructions. ENA first public 2021-07-30 ENA last update 2021-07-30 External Id SAMEA9405882 INSDC center alias Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC center name Department of Immunology, Institute of Biomedical Science, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. INSDC first public 2021-07-30T00:22:22Z INSDC last update 2021-07-30T00:22:22Z INSDC status public Submitter Id E-MTAB-10732:Sample 9 age 8 to 12 broker name ArrayExpress cell type neutrophil common name house mouse genotype wild type genotype organism part bone marrow sample name E-MTAB-10732:Sample 9 sex female strain C57BL/6