ERS7121561 SAMEA9405884 10090 Mus musculus Protocols: C57BL/6 pancreas cell line B6panc expressing full length Ova were either non-treated or given different amounts of OT-I CD8 T cells. Surviving cells were harvested and sequenced. C57BL/6 mouse pancreas cells were transduced with EF1 promoter driven full-length chicken ovalbumin (Ovalbumin cDNA from Addgene # 64599) and selected with blasticidin (Thermo cat# A11139-03). Cells were treated with 20 ng murine interferon gamma (Peprotech, cat#315-05) for 16 h and stained with a PE anti-mouse H-2Kb bound to SIINFEKL Antibody (Biolegend, cat# 141603). The top 10% of cells that stained positively for SIINFEKL presentation were sorted by flow (Sony MA900). Following a week of passaging, the sorted cells were again treated with 20 ng murine interferon gamma and the top 2% of cells positive for SIINFEKL presentation were sorted. This population of cells was used for all screens. phenol chloroform custom primers for sgRNA amplicon ENA first public 2021-10-01 ENA last update 2021-10-01 External Id SAMEA9405884 INSDC center alias HMS BWH INSDC center name HMS BWH INSDC first public 2021-10-01T00:24:52Z INSDC last update 2021-10-01T00:24:52Z INSDC status public Submitter Id E-MTAB-10724:0.1_1_rep1 broker name ArrayExpress cell line B6panc coculture coculture 1 to 10 with OT-I CD8 T cell common name house mouse developmental stage adult genotype OVA full length organism part pancreas sample name E-MTAB-10724:0.1_1_rep1 strain C57BL/6J ERS7121562 SAMEA9405885 10090 Mus musculus Protocols: C57BL/6 pancreas cell line B6panc expressing full length Ova were either non-treated or given different amounts of OT-I CD8 T cells. Surviving cells were harvested and sequenced. C57BL/6 mouse pancreas cells were transduced with EF1 promoter driven full-length chicken ovalbumin (Ovalbumin cDNA from Addgene # 64599) and selected with blasticidin (Thermo cat# A11139-03). Cells were treated with 20 ng murine interferon gamma (Peprotech, cat#315-05) for 16 h and stained with a PE anti-mouse H-2Kb bound to SIINFEKL Antibody (Biolegend, cat# 141603). The top 10% of cells that stained positively for SIINFEKL presentation were sorted by flow (Sony MA900). Following a week of passaging, the sorted cells were again treated with 20 ng murine interferon gamma and the top 2% of cells positive for SIINFEKL presentation were sorted. This population of cells was used for all screens. phenol chloroform custom primers for sgRNA amplicon ENA first public 2021-10-01 ENA last update 2021-10-01 External Id SAMEA9405885 INSDC center alias HMS BWH INSDC center name HMS BWH INSDC first public 2021-10-01T00:24:52Z INSDC last update 2021-10-01T00:24:52Z INSDC status public Submitter Id E-MTAB-10724:0.1_1_rep2 broker name ArrayExpress cell line B6panc coculture coculture 1 to 10 with OT-I CD8 T cell common name house mouse developmental stage adult genotype OVA full length organism part pancreas sample name E-MTAB-10724:0.1_1_rep2 strain C57BL/6J ERS7121563 SAMEA9405886 10090 Mus musculus Protocols: C57BL/6 pancreas cell line B6panc expressing full length Ova were either non-treated or given different amounts of OT-I CD8 T cells. Surviving cells were harvested and sequenced. C57BL/6 mouse pancreas cells were transduced with EF1 promoter driven full-length chicken ovalbumin (Ovalbumin cDNA from Addgene # 64599) and selected with blasticidin (Thermo cat# A11139-03). Cells were treated with 20 ng murine interferon gamma (Peprotech, cat#315-05) for 16 h and stained with a PE anti-mouse H-2Kb bound to SIINFEKL Antibody (Biolegend, cat# 141603). The top 10% of cells that stained positively for SIINFEKL presentation were sorted by flow (Sony MA900). Following a week of passaging, the sorted cells were again treated with 20 ng murine interferon gamma and the top 2% of cells positive for SIINFEKL presentation were sorted. This population of cells was used for all screens. phenol chloroform custom primers for sgRNA amplicon ENA first public 2021-10-01 ENA last update 2021-10-01 External Id SAMEA9405886 INSDC center alias HMS BWH INSDC center name HMS BWH INSDC first public 2021-10-01T00:24:52Z INSDC last update 2021-10-01T00:24:52Z INSDC status public Submitter Id E-MTAB-10724:0.1_1_rep3 broker name ArrayExpress cell line B6panc coculture coculture 1 to 10 with OT-I CD8 T cell common name house mouse developmental stage adult genotype OVA full length organism part pancreas sample name E-MTAB-10724:0.1_1_rep3 strain C57BL/6J ERS7121564 SAMEA9405889 10090 Mus musculus Protocols: C57BL/6 pancreas cell line B6panc expressing full length Ova were either non-treated or given different amounts of OT-I CD8 T cells. Surviving cells were harvested and sequenced. C57BL/6 mouse pancreas cells were transduced with EF1 promoter driven full-length chicken ovalbumin (Ovalbumin cDNA from Addgene # 64599) and selected with blasticidin (Thermo cat# A11139-03). Cells were treated with 20 ng murine interferon gamma (Peprotech, cat#315-05) for 16 h and stained with a PE anti-mouse H-2Kb bound to SIINFEKL Antibody (Biolegend, cat# 141603). The top 10% of cells that stained positively for SIINFEKL presentation were sorted by flow (Sony MA900). Following a week of passaging, the sorted cells were again treated with 20 ng murine interferon gamma and the top 2% of cells positive for SIINFEKL presentation were sorted. This population of cells was used for all screens. phenol chloroform custom primers for sgRNA amplicon ENA first public 2021-10-01 ENA last update 2021-10-01 External Id SAMEA9405889 INSDC center alias HMS BWH INSDC center name HMS BWH INSDC first public 2021-10-01T00:24:52Z INSDC last update 2021-10-01T00:24:52Z INSDC status public Submitter Id E-MTAB-10724:0.25_1_rep1 broker name ArrayExpress cell line B6panc coculture coculture 1 to 4 with OT-I CD8 T cell common name house mouse developmental stage adult genotype OVA full length organism part pancreas sample name E-MTAB-10724:0.25_1_rep1 strain C57BL/6J ERS7121565 SAMEA9405887 10090 Mus musculus Protocols: C57BL/6 pancreas cell line B6panc expressing full length Ova were either non-treated or given different amounts of OT-I CD8 T cells. Surviving cells were harvested and sequenced. C57BL/6 mouse pancreas cells were transduced with EF1 promoter driven full-length chicken ovalbumin (Ovalbumin cDNA from Addgene # 64599) and selected with blasticidin (Thermo cat# A11139-03). Cells were treated with 20 ng murine interferon gamma (Peprotech, cat#315-05) for 16 h and stained with a PE anti-mouse H-2Kb bound to SIINFEKL Antibody (Biolegend, cat# 141603). The top 10% of cells that stained positively for SIINFEKL presentation were sorted by flow (Sony MA900). Following a week of passaging, the sorted cells were again treated with 20 ng murine interferon gamma and the top 2% of cells positive for SIINFEKL presentation were sorted. This population of cells was used for all screens. phenol chloroform custom primers for sgRNA amplicon ENA first public 2021-10-01 ENA last update 2021-10-01 External Id SAMEA9405887 INSDC center alias HMS BWH INSDC center name HMS BWH INSDC first public 2021-10-01T00:24:52Z INSDC last update 2021-10-01T00:24:52Z INSDC status public Submitter Id E-MTAB-10724:0.25_1_rep2 broker name ArrayExpress cell line B6panc coculture coculture 1 to 4 with OT-I CD8 T cell common name house mouse developmental stage adult genotype OVA full length organism part pancreas sample name E-MTAB-10724:0.25_1_rep2 strain C57BL/6J ERS7121566 SAMEA9405888 10090 Mus musculus Protocols: C57BL/6 pancreas cell line B6panc expressing full length Ova were either non-treated or given different amounts of OT-I CD8 T cells. Surviving cells were harvested and sequenced. C57BL/6 mouse pancreas cells were transduced with EF1 promoter driven full-length chicken ovalbumin (Ovalbumin cDNA from Addgene # 64599) and selected with blasticidin (Thermo cat# A11139-03). Cells were treated with 20 ng murine interferon gamma (Peprotech, cat#315-05) for 16 h and stained with a PE anti-mouse H-2Kb bound to SIINFEKL Antibody (Biolegend, cat# 141603). The top 10% of cells that stained positively for SIINFEKL presentation were sorted by flow (Sony MA900). Following a week of passaging, the sorted cells were again treated with 20 ng murine interferon gamma and the top 2% of cells positive for SIINFEKL presentation were sorted. This population of cells was used for all screens. phenol chloroform custom primers for sgRNA amplicon ENA first public 2021-10-01 ENA last update 2021-10-01 External Id SAMEA9405888 INSDC center alias HMS BWH INSDC center name HMS BWH INSDC first public 2021-10-01T00:24:52Z INSDC last update 2021-10-01T00:24:52Z INSDC status public Submitter Id E-MTAB-10724:0.25_1_rep3 broker name ArrayExpress cell line B6panc coculture coculture 1 to 4 with OT-I CD8 T cell common name house mouse developmental stage adult genotype OVA full length organism part pancreas sample name E-MTAB-10724:0.25_1_rep3 strain C57BL/6J ERS7121567 SAMEA9405890 10090 Mus musculus Protocols: C57BL/6 pancreas cell line B6panc expressing full length Ova were either non-treated or given different amounts of OT-I CD8 T cells. Surviving cells were harvested and sequenced. C57BL/6 mouse pancreas cells were transduced with EF1 promoter driven full-length chicken ovalbumin (Ovalbumin cDNA from Addgene # 64599) and selected with blasticidin (Thermo cat# A11139-03). Cells were treated with 20 ng murine interferon gamma (Peprotech, cat#315-05) for 16 h and stained with a PE anti-mouse H-2Kb bound to SIINFEKL Antibody (Biolegend, cat# 141603). The top 10% of cells that stained positively for SIINFEKL presentation were sorted by flow (Sony MA900). Following a week of passaging, the sorted cells were again treated with 20 ng murine interferon gamma and the top 2% of cells positive for SIINFEKL presentation were sorted. This population of cells was used for all screens. phenol chloroform custom primers for sgRNA amplicon ENA first public 2021-10-01 ENA last update 2021-10-01 External Id SAMEA9405890 INSDC center alias HMS BWH INSDC center name HMS BWH INSDC first public 2021-10-01T00:24:52Z INSDC last update 2021-10-01T00:24:52Z INSDC status public Submitter Id E-MTAB-10724:No_T_Cells_rep1 broker name ArrayExpress cell line B6panc coculture monoculture common name house mouse developmental stage adult genotype OVA full length organism part pancreas sample name E-MTAB-10724:No_T_Cells_rep1 strain C57BL/6J ERS7121568 SAMEA9405891 10090 Mus musculus Protocols: C57BL/6 pancreas cell line B6panc expressing full length Ova were either non-treated or given different amounts of OT-I CD8 T cells. Surviving cells were harvested and sequenced. C57BL/6 mouse pancreas cells were transduced with EF1 promoter driven full-length chicken ovalbumin (Ovalbumin cDNA from Addgene # 64599) and selected with blasticidin (Thermo cat# A11139-03). Cells were treated with 20 ng murine interferon gamma (Peprotech, cat#315-05) for 16 h and stained with a PE anti-mouse H-2Kb bound to SIINFEKL Antibody (Biolegend, cat# 141603). The top 10% of cells that stained positively for SIINFEKL presentation were sorted by flow (Sony MA900). Following a week of passaging, the sorted cells were again treated with 20 ng murine interferon gamma and the top 2% of cells positive for SIINFEKL presentation were sorted. This population of cells was used for all screens. phenol chloroform custom primers for sgRNA amplicon ENA first public 2021-10-01 ENA last update 2021-10-01 External Id SAMEA9405891 INSDC center alias HMS BWH INSDC center name HMS BWH INSDC first public 2021-10-01T00:24:52Z INSDC last update 2021-10-01T00:24:52Z INSDC status public Submitter Id E-MTAB-10724:No_T_Cells_rep2 broker name ArrayExpress cell line B6panc coculture monoculture common name house mouse developmental stage adult genotype OVA full length organism part pancreas sample name E-MTAB-10724:No_T_Cells_rep2 strain C57BL/6J ERS7121569 SAMEA9405892 10090 Mus musculus Protocols: C57BL/6 pancreas cell line B6panc expressing full length Ova were either non-treated or given different amounts of OT-I CD8 T cells. Surviving cells were harvested and sequenced. C57BL/6 mouse pancreas cells were transduced with EF1 promoter driven full-length chicken ovalbumin (Ovalbumin cDNA from Addgene # 64599) and selected with blasticidin (Thermo cat# A11139-03). Cells were treated with 20 ng murine interferon gamma (Peprotech, cat#315-05) for 16 h and stained with a PE anti-mouse H-2Kb bound to SIINFEKL Antibody (Biolegend, cat# 141603). The top 10% of cells that stained positively for SIINFEKL presentation were sorted by flow (Sony MA900). Following a week of passaging, the sorted cells were again treated with 20 ng murine interferon gamma and the top 2% of cells positive for SIINFEKL presentation were sorted. This population of cells was used for all screens. phenol chloroform custom primers for sgRNA amplicon ENA first public 2021-10-01 ENA last update 2021-10-01 External Id SAMEA9405892 INSDC center alias HMS BWH INSDC center name HMS BWH INSDC first public 2021-10-01T00:24:52Z INSDC last update 2021-10-01T00:24:52Z INSDC status public Submitter Id E-MTAB-10724:No_T_Cells_rep3 broker name ArrayExpress cell line B6panc coculture monoculture common name house mouse developmental stage adult genotype OVA full length organism part pancreas sample name E-MTAB-10724:No_T_Cells_rep3 strain C57BL/6J