ERS942529 SAMEA3635380 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:42Z External Id SAMEA3635380 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:42Z INSDC status public Submitter Id E-MTAB-3991:Sample 1 broker name ArrayExpress common name baker's yeast genotype HIS3, LEU2, URA3, MET15 knockout sample name E-MTAB-3991:Sample 1 scientific_name Saccharomyces cerevisiae strain HLUM ERS942530 SAMEA3635381 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:42Z External Id SAMEA3635381 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:42Z INSDC status public Submitter Id E-MTAB-3991:Sample 10 broker name ArrayExpress common name baker's yeast genotype HIS3, LEU2, MET15 knockout sample name E-MTAB-3991:Sample 10 scientific_name Saccharomyces cerevisiae strain HLM ERS942531 SAMEA3635382 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:42Z External Id SAMEA3635382 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:42Z INSDC status public Submitter Id E-MTAB-3991:Sample 11 broker name ArrayExpress common name baker's yeast genotype HIS3, LEU2, MET15 knockout sample name E-MTAB-3991:Sample 11 scientific_name Saccharomyces cerevisiae strain HLM ERS942533 SAMEA3635384 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:42Z External Id SAMEA3635384 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:42Z INSDC status public Submitter Id E-MTAB-3991:Sample 13 broker name ArrayExpress common name baker's yeast genotype HIS3, URA3, MET15 knockout sample name E-MTAB-3991:Sample 13 scientific_name Saccharomyces cerevisiae strain HUM ERS942535 SAMEA3635386 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:47Z External Id SAMEA3635386 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:47Z INSDC status public Submitter Id E-MTAB-3991:Sample 15 broker name ArrayExpress common name baker's yeast genotype HIS3, URA3, MET15 knockout sample name E-MTAB-3991:Sample 15 scientific_name Saccharomyces cerevisiae strain HUM ERS942537 SAMEA3635388 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:47Z External Id SAMEA3635388 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:47Z INSDC status public Submitter Id E-MTAB-3991:Sample 17 broker name ArrayExpress common name baker's yeast genotype HIS3, URA3, MET15 knockout sample name E-MTAB-3991:Sample 17 scientific_name Saccharomyces cerevisiae strain HUM ERS942539 SAMEA3635390 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:47Z External Id SAMEA3635390 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:47Z INSDC status public Submitter Id E-MTAB-3991:Sample 19 broker name ArrayExpress common name baker's yeast genotype LEU2, URA3, MET15 knockout sample name E-MTAB-3991:Sample 19 scientific_name Saccharomyces cerevisiae strain LUM ERS942542 SAMEA3635393 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:52Z External Id SAMEA3635393 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:52Z INSDC status public Submitter Id E-MTAB-3991:Sample 21 broker name ArrayExpress common name baker's yeast genotype LEU2, URA3, MET15 knockout sample name E-MTAB-3991:Sample 21 scientific_name Saccharomyces cerevisiae strain LUM ERS942544 SAMEA3635395 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:52Z External Id SAMEA3635395 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:52Z INSDC status public Submitter Id E-MTAB-3991:Sample 23 broker name ArrayExpress common name baker's yeast genotype LEU2, URA3, MET15 knockout sample name E-MTAB-3991:Sample 23 scientific_name Saccharomyces cerevisiae strain LUM ERS942548 SAMEA3635399 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:57Z External Id SAMEA3635399 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:57Z INSDC status public Submitter Id E-MTAB-3991:Sample 27 broker name ArrayExpress common name baker's yeast genotype HIS3, LEU2, URA3 knockout sample name E-MTAB-3991:Sample 27 scientific_name Saccharomyces cerevisiae strain HLU ERS942550 SAMEA3635401 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:57Z External Id SAMEA3635401 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:57Z INSDC status public Submitter Id E-MTAB-3991:Sample 29 broker name ArrayExpress common name baker's yeast genotype HIS3, LEU2, URA3 knockout sample name E-MTAB-3991:Sample 29 scientific_name Saccharomyces cerevisiae strain HLU ERS942551 SAMEA3635402 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:57Z External Id SAMEA3635402 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:57Z INSDC status public Submitter Id E-MTAB-3991:Sample 3 broker name ArrayExpress common name baker's yeast genotype HIS3, LEU2, URA3, MET15 knockout sample name E-MTAB-3991:Sample 3 scientific_name Saccharomyces cerevisiae strain HLUM ERS942553 SAMEA3635404 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:02Z External Id SAMEA3635404 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:02Z INSDC status public Submitter Id E-MTAB-3991:Sample 31 broker name ArrayExpress common name baker's yeast genotype HIS3, MET15 knockout sample name E-MTAB-3991:Sample 31 scientific_name Saccharomyces cerevisiae strain HM ERS942555 SAMEA3635406 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:02Z External Id SAMEA3635406 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:02Z INSDC status public Submitter Id E-MTAB-3991:Sample 33 broker name ArrayExpress common name baker's yeast genotype HIS3, MET15 knockout sample name E-MTAB-3991:Sample 33 scientific_name Saccharomyces cerevisiae strain HM ERS942557 SAMEA3635408 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:02Z External Id SAMEA3635408 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:02Z INSDC status public Submitter Id E-MTAB-3991:Sample 35 broker name ArrayExpress common name baker's yeast genotype HIS3, MET15 knockout sample name E-MTAB-3991:Sample 35 scientific_name Saccharomyces cerevisiae strain HM ERS942559 SAMEA3635410 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:08Z External Id SAMEA3635410 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:08Z INSDC status public Submitter Id E-MTAB-3991:Sample 37 broker name ArrayExpress common name baker's yeast genotype LEU2, MET15 knockout sample name E-MTAB-3991:Sample 37 scientific_name Saccharomyces cerevisiae strain LM ERS942561 SAMEA3635412 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:08Z External Id SAMEA3635412 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:08Z INSDC status public Submitter Id E-MTAB-3991:Sample 39 broker name ArrayExpress common name baker's yeast genotype LEU2, MET15 knockout sample name E-MTAB-3991:Sample 39 scientific_name Saccharomyces cerevisiae strain LM ERS942564 SAMEA3635415 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:08Z External Id SAMEA3635415 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:08Z INSDC status public Submitter Id E-MTAB-3991:Sample 41 broker name ArrayExpress common name baker's yeast genotype LEU2, MET15 knockout sample name E-MTAB-3991:Sample 41 scientific_name Saccharomyces cerevisiae strain LM ERS942566 SAMEA3635417 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:12Z External Id SAMEA3635417 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:12Z INSDC status public Submitter Id E-MTAB-3991:Sample 43 broker name ArrayExpress common name baker's yeast genotype URA3, MET15 knockout sample name E-MTAB-3991:Sample 43 scientific_name Saccharomyces cerevisiae strain UM ERS942568 SAMEA3635419 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:12Z External Id SAMEA3635419 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:12Z INSDC status public Submitter Id E-MTAB-3991:Sample 45 broker name ArrayExpress common name baker's yeast genotype URA3, MET15 knockout sample name E-MTAB-3991:Sample 45 scientific_name Saccharomyces cerevisiae strain UM ERS942570 SAMEA3635421 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:12Z External Id SAMEA3635421 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:12Z INSDC status public Submitter Id E-MTAB-3991:Sample 47 broker name ArrayExpress common name baker's yeast genotype URA3, MET15 knockout sample name E-MTAB-3991:Sample 47 scientific_name Saccharomyces cerevisiae strain UM ERS942572 SAMEA3635423 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:16Z External Id SAMEA3635423 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:16Z INSDC status public Submitter Id E-MTAB-3991:Sample 49 broker name ArrayExpress common name baker's yeast genotype HIS3, LEU2 knockout sample name E-MTAB-3991:Sample 49 scientific_name Saccharomyces cerevisiae strain UM ERS942573 SAMEA3635424 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:16Z External Id SAMEA3635424 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:16Z INSDC status public Submitter Id E-MTAB-3991:Sample 5 broker name ArrayExpress common name baker's yeast genotype HIS3, LEU2, URA3, MET15 knockout sample name E-MTAB-3991:Sample 5 scientific_name Saccharomyces cerevisiae strain HLUM ERS942575 SAMEA3635426 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:16Z External Id SAMEA3635426 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:16Z INSDC status public Submitter Id E-MTAB-3991:Sample 51 broker name ArrayExpress common name baker's yeast genotype HIS3, LEU2 knockout sample name E-MTAB-3991:Sample 51 scientific_name Saccharomyces cerevisiae strain UM ERS942577 SAMEA3635428 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:21Z External Id SAMEA3635428 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:21Z INSDC status public Submitter Id E-MTAB-3991:Sample 53 broker name ArrayExpress common name baker's yeast genotype HIS3, LEU2 knockout sample name E-MTAB-3991:Sample 53 scientific_name Saccharomyces cerevisiae strain UM ERS942579 SAMEA3635430 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:21Z External Id SAMEA3635430 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:21Z INSDC status public Submitter Id E-MTAB-3991:Sample 55 broker name ArrayExpress common name baker's yeast genotype HIS3, URA3 knockout sample name E-MTAB-3991:Sample 55 scientific_name Saccharomyces cerevisiae strain UM ERS942581 SAMEA3635432 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:21Z External Id SAMEA3635432 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:21Z INSDC status public Submitter Id E-MTAB-3991:Sample 57 broker name ArrayExpress common name baker's yeast genotype HIS3, URA3 knockout sample name E-MTAB-3991:Sample 57 scientific_name Saccharomyces cerevisiae strain UM ERS942583 SAMEA3635434 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:26Z External Id SAMEA3635434 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:26Z INSDC status public Submitter Id E-MTAB-3991:Sample 59 broker name ArrayExpress common name baker's yeast genotype HIS3, URA3 knockout sample name E-MTAB-3991:Sample 59 scientific_name Saccharomyces cerevisiae strain UM ERS942586 SAMEA3635437 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:26Z External Id SAMEA3635437 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:26Z INSDC status public Submitter Id E-MTAB-3991:Sample 61 broker name ArrayExpress common name baker's yeast genotype LEU2, URA3 knockout sample name E-MTAB-3991:Sample 61 scientific_name Saccharomyces cerevisiae strain UM ERS942588 SAMEA3635439 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:26Z External Id SAMEA3635439 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:26Z INSDC status public Submitter Id E-MTAB-3991:Sample 63 broker name ArrayExpress common name baker's yeast genotype LEU2, URA3 knockout sample name E-MTAB-3991:Sample 63 scientific_name Saccharomyces cerevisiae strain UM ERS942590 SAMEA3635441 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:32Z External Id SAMEA3635441 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:32Z INSDC status public Submitter Id E-MTAB-3991:Sample 65 broker name ArrayExpress common name baker's yeast genotype LEU2, URA3 knockout sample name E-MTAB-3991:Sample 65 scientific_name Saccharomyces cerevisiae strain UM ERS942592 SAMEA3635443 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:32Z External Id SAMEA3635443 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:32Z INSDC status public Submitter Id E-MTAB-3991:Sample 67 broker name ArrayExpress common name baker's yeast genotype MET15 knockout sample name E-MTAB-3991:Sample 67 scientific_name Saccharomyces cerevisiae strain UM ERS942546 SAMEA3635397 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:52Z External Id SAMEA3635397 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:52Z INSDC status public Submitter Id E-MTAB-3991:Sample 25 broker name ArrayExpress common name baker's yeast genotype HIS3, LEU2, URA3 knockout sample name E-MTAB-3991:Sample 25 scientific_name Saccharomyces cerevisiae strain HLU ERS942595 SAMEA3635446 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:26Z ENA-LAST-UPDATE 2018-03-09T09:26:37Z External Id SAMEA3635446 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:26Z INSDC last update 2018-03-09T09:26:37Z INSDC status public Submitter Id E-MTAB-3991:Sample 7 broker name ArrayExpress common name baker's yeast genotype HIS3, LEU2, MET15 knockout sample name E-MTAB-3991:Sample 7 scientific_name Saccharomyces cerevisiae strain HLM ERS942597 SAMEA3635448 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:26Z ENA-LAST-UPDATE 2018-03-09T09:26:37Z External Id SAMEA3635448 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:26Z INSDC last update 2018-03-09T09:26:37Z INSDC status public Submitter Id E-MTAB-3991:Sample 71 broker name ArrayExpress common name baker's yeast genotype MET15 knockout sample name E-MTAB-3991:Sample 71 scientific_name Saccharomyces cerevisiae strain UM ERS942599 SAMEA3635450 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:26Z ENA-LAST-UPDATE 2018-03-09T09:26:37Z External Id SAMEA3635450 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:26Z INSDC last update 2018-03-09T09:26:37Z INSDC status public Submitter Id E-MTAB-3991:Sample 73 broker name ArrayExpress common name baker's yeast genotype HIS3 knockout sample name E-MTAB-3991:Sample 73 scientific_name Saccharomyces cerevisiae strain UM ERS942601 SAMEA3635452 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:26Z ENA-LAST-UPDATE 2018-03-09T09:26:43Z External Id SAMEA3635452 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:26Z INSDC last update 2018-03-09T09:26:43Z INSDC status public Submitter Id E-MTAB-3991:Sample 75 broker name ArrayExpress common name baker's yeast genotype HIS3 knockout sample name E-MTAB-3991:Sample 75 scientific_name Saccharomyces cerevisiae strain UM ERS942603 SAMEA3635454 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:26Z ENA-LAST-UPDATE 2018-03-09T09:26:43Z External Id SAMEA3635454 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:26Z INSDC last update 2018-03-09T09:26:43Z INSDC status public Submitter Id E-MTAB-3991:Sample 77 broker name ArrayExpress common name baker's yeast genotype HIS3 knockout sample name E-MTAB-3991:Sample 77 scientific_name Saccharomyces cerevisiae strain UM ERS942605 SAMEA3635456 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:26Z ENA-LAST-UPDATE 2018-03-09T09:26:43Z External Id SAMEA3635456 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:26Z INSDC last update 2018-03-09T09:26:43Z INSDC status public Submitter Id E-MTAB-3991:Sample 79 broker name ArrayExpress common name baker's yeast genotype LEU2 knockout sample name E-MTAB-3991:Sample 79 scientific_name Saccharomyces cerevisiae strain UM ERS942608 SAMEA3635459 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:26Z ENA-LAST-UPDATE 2018-03-09T09:26:49Z External Id SAMEA3635459 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:26Z INSDC last update 2018-03-09T09:26:49Z INSDC status public Submitter Id E-MTAB-3991:Sample 81 broker name ArrayExpress common name baker's yeast genotype LEU2 knockout sample name E-MTAB-3991:Sample 81 scientific_name Saccharomyces cerevisiae strain UM ERS942610 SAMEA3635461 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:26Z ENA-LAST-UPDATE 2018-03-09T09:26:49Z External Id SAMEA3635461 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:26Z INSDC last update 2018-03-09T09:26:49Z INSDC status public Submitter Id E-MTAB-3991:Sample 83 broker name ArrayExpress common name baker's yeast genotype LEU2 knockout sample name E-MTAB-3991:Sample 83 scientific_name Saccharomyces cerevisiae strain UM ERS942612 SAMEA3635463 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:26Z ENA-LAST-UPDATE 2018-03-09T09:26:49Z External Id SAMEA3635463 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:26Z INSDC last update 2018-03-09T09:26:49Z INSDC status public Submitter Id E-MTAB-3991:Sample 85 broker name ArrayExpress common name baker's yeast genotype URA3 knockout sample name E-MTAB-3991:Sample 85 scientific_name Saccharomyces cerevisiae strain UM ERS942614 SAMEA3635465 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:32Z External Id SAMEA3635465 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:32Z INSDC status public Submitter Id E-MTAB-3991:Sample 87 broker name ArrayExpress common name baker's yeast genotype URA3 knockout sample name E-MTAB-3991:Sample 87 scientific_name Saccharomyces cerevisiae strain UM ERS942616 SAMEA3635467 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:32Z External Id SAMEA3635467 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:32Z INSDC status public Submitter Id E-MTAB-3991:Sample 89 broker name ArrayExpress common name baker's yeast genotype URA3 knockout sample name E-MTAB-3991:Sample 89 scientific_name Saccharomyces cerevisiae strain UM ERS942619 SAMEA3635470 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:37Z External Id SAMEA3635470 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:37Z INSDC status public Submitter Id E-MTAB-3991:Sample 91 broker name ArrayExpress common name baker's yeast genotype PROTOTROPH sample name E-MTAB-3991:Sample 91 scientific_name Saccharomyces cerevisiae strain UM ERS942621 SAMEA3635472 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:37Z External Id SAMEA3635472 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:37Z INSDC status public Submitter Id E-MTAB-3991:Sample 93 broker name ArrayExpress common name baker's yeast genotype PROTOTROPH sample name E-MTAB-3991:Sample 93 scientific_name Saccharomyces cerevisiae strain UM ERS942623 SAMEA3635474 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:25:37Z External Id SAMEA3635474 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:25:37Z INSDC status public Submitter Id E-MTAB-3991:Sample 95 broker name ArrayExpress common name baker's yeast genotype PROTOTROPH sample name E-MTAB-3991:Sample 95 scientific_name Saccharomyces cerevisiae strain UM ERS942594 SAMEA3635445 4932 Saccharomyces cerevisiae Protocols: S. cerevisiae strains were transferred from cryo-preserved cultures to agar dishes containing drop-in media and grown for 2 days at 30C. Pre-cultures were inoculated in 10 ml drop-in media and grown for 30 h at 30C. The cultures were washed with H2O and the main-cultures (n=5) inoculated at 0.15 OD600 in 200 l synthetic complete (SC) media in a 96-well plate. Growth was recorded for 35 hour for every 14 min by measuring OD595 using a FLUOstar OPTIMA (BMG Labtech) plate reader (87 rpm, 30C). Main-cultures were inoculated in 50 ml synthetic complete media at a starting OD600 of 0.15 (30C, 180 rpm). Strains were then grown for 6-19 hour until an OD600 of 0.8, the cells collected by centrifugation (2 min, 3000 g) and the supernatant discarded. The pellet was re-suspended in 1 ml H2O and three aliquots prepared with 350 l each. The cells were collected (0.5 min, 5000 g), the supernatant removed with a pipette and the sample snap frozen and stored at 80C until further processing. RNA for transcriptomics was prepared using the yeast RNA mini kit (Zymo Research) followed by DNase treatment, and processed using the TruSeq RNA library preparation kit (Illumina) following the manufacturers instructions ENA-FIRST-PUBLIC 2015-12-07T17:01:25Z ENA-LAST-UPDATE 2018-03-09T09:26:32Z External Id SAMEA3635445 INSDC center name Ralser Group, Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Sanger Building, CB2 1GA, Cambridge,a United Kingdom. INSDC first public 2015-12-07T17:01:25Z INSDC last update 2018-03-09T09:26:32Z INSDC status public Submitter Id E-MTAB-3991:Sample 69 broker name ArrayExpress common name baker's yeast genotype MET15 knockout sample name E-MTAB-3991:Sample 69 scientific_name Saccharomyces cerevisiae strain UM ERS942532 SAMEA4084136 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 12 genotype HIS3, LEU2, MET15 knockout strain HLM ERS942534 SAMEA4084083 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 14 genotype HIS3, URA3, MET15 knockout strain HUM ERS942536 SAMEA4084122 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 16 genotype HIS3, URA3, MET15 knockout strain HUM ERS942538 SAMEA4084164 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 18 genotype HIS3, URA3, MET15 knockout strain HUM ERS942540 SAMEA4084135 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 2 genotype HIS3, LEU2, URA3, MET15 knockout strain HLUM ERS942541 SAMEA4084148 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 20 genotype LEU2, URA3, MET15 knockout strain LUM ERS942543 SAMEA4084093 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 22 genotype LEU2, URA3, MET15 knockout strain LUM ERS942547 SAMEA4084080 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 26 genotype HIS3, LEU2, URA3 knockout strain HLU ERS942545 SAMEA4084132 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 24 genotype LEU2, URA3, MET15 knockout strain LUM ERS942549 SAMEA4084119 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 28 genotype HIS3, LEU2, URA3 knockout strain HLU ERS942552 SAMEA4084104 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 30 genotype HIS3, LEU2, URA3 knockout strain HLU ERS942554 SAMEA4084143 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 32 genotype HIS3, MET15 knockout strain HM ERS942556 SAMEA4084089 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 34 genotype HIS3, MET15 knockout strain HM ERS942558 SAMEA4084128 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 36 genotype HIS3, MET15 knockout strain HM ERS942560 SAMEA4084077 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 38 genotype LEU2, MET15 knockout strain LM ERS942562 SAMEA4084074 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 4 genotype HIS3, LEU2, URA3, MET15 knockout strain HLUM ERS942563 SAMEA4084156 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 40 genotype LEU2, MET15 knockout strain LM ERS942565 SAMEA4084100 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 42 genotype LEU2, MET15 knockout strain LM ERS942567 SAMEA4084140 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 44 genotype URA3, MET15 knockout strain UM ERS942569 SAMEA4084086 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 46 genotype URA3, MET15 knockout strain UM ERS942571 SAMEA4084126 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 48 genotype URA3, MET15 knockout strain UM ERS942574 SAMEA4084110 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 50 genotype HIS3, LEU2 knockout strain UM ERS942578 SAMEA4084098 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 54 genotype HIS3, LEU2 knockout strain UM ERS942576 SAMEA4084152 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 52 genotype HIS3, LEU2 knockout strain UM ERS942580 SAMEA4084137 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 56 genotype HIS3, URA3 knockout strain UM ERS942582 SAMEA4084084 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 58 genotype HIS3, URA3 knockout strain UM ERS942584 SAMEA4084151 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 6 genotype HIS3, LEU2, URA3, MET15 knockout strain HLUM ERS942585 SAMEA4084070 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 60 genotype HIS3, URA3 knockout strain UM ERS942587 SAMEA4084107 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 62 genotype LEU2, URA3 knockout strain UM ERS942589 SAMEA4084149 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 64 genotype LEU2, URA3 knockout strain UM ERS942591 SAMEA4084094 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 66 genotype LEU2, URA3 knockout strain UM ERS942593 SAMEA4084133 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 68 genotype MET15 knockout strain UM ERS942596 SAMEA4084118 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 70 genotype MET15 knockout strain UM ERS942598 SAMEA4084161 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 72 genotype MET15 knockout strain UM ERS942600 SAMEA4084105 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 74 genotype HIS3 knockout strain UM ERS942602 SAMEA4084144 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 76 genotype HIS3 knockout strain UM ERS942604 SAMEA4084090 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 78 genotype HIS3 knockout strain UM ERS942606 SAMEA4084112 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 8 genotype HIS3, LEU2, MET15 knockout strain HLM ERS942607 SAMEA4084078 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 80 genotype LEU2 knockout strain UM ERS942611 SAMEA4084157 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 84 genotype LEU2 knockout strain UM ERS942609 SAMEA4084115 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 82 genotype LEU2 knockout strain UM ERS942613 SAMEA4084101 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 86 genotype URA3 knockout strain UM ERS942615 SAMEA4084141 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 88 genotype URA3 knockout strain UM ERS942617 SAMEA4084069 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 9 genotype HIS3, LEU2, MET15 knockout strain HLM ERS942618 SAMEA4084125 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 90 genotype URA3 knockout strain UM ERS942620 SAMEA4084073 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 92 genotype PROTOTROPH strain UM ERS942622 SAMEA4084111 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 94 genotype PROTOTROPH strain UM ERS942624 SAMEA4084153 4932 Saccharomyces cerevisiae baker''s yeast Sample Name source Sample 96 genotype PROTOTROPH strain UM