ERS7147476 SAMEA9424249 9606 Homo sapiens Protocols: Cultured breast cancer cells were harvested, and pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation Total RNA samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit. Protocol was modified based on the quality and quality of the samples. Customized adapters with unique molecular indexes (UMI) and Sample-specific dual-indexes primers were added to each library. The quantity of libraries was measured with Qubit and quality measured by TapStation Genomic DNA Assay. ENA-FIRST-PUBLIC 2022-07-05 ENA-LAST-UPDATE 2022-07-05 External Id SAMEA9424249 INSDC center alias Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC center name Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC first public 2022-07-05T12:03:08Z INSDC last update 2022-07-05T12:03:08Z INSDC status public Submitter Id E-MTAB-10733:MDA-MB-231_#1 broker name ArrayExpress cell_line MDAMB231 common name human developmental stage adult disease adenocarcinoma growth condition in vitro immunophenotype ER negative isolate not applicable organism part mammary gland sample name E-MTAB-10733:MDA-MB-231_#1 ERS7147477 SAMEA9424250 9606 Homo sapiens Protocols: Cultured breast cancer cells were harvested, and pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation Total RNA samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit. Protocol was modified based on the quality and quality of the samples. Customized adapters with unique molecular indexes (UMI) and Sample-specific dual-indexes primers were added to each library. The quantity of libraries was measured with Qubit and quality measured by TapStation Genomic DNA Assay. ENA-FIRST-PUBLIC 2022-07-05 ENA-LAST-UPDATE 2022-07-05 External Id SAMEA9424250 INSDC center alias Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC center name Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC first public 2022-07-05T12:03:08Z INSDC last update 2022-07-05T12:03:08Z INSDC status public Submitter Id E-MTAB-10733:MDA-MB-231_#2 broker name ArrayExpress cell_line MDAMB231 common name human developmental stage adult disease adenocarcinoma growth condition in vitro immunophenotype ER negative isolate not applicable organism part mammary gland sample name E-MTAB-10733:MDA-MB-231_#2 ERS7147478 SAMEA9424251 9606 Homo sapiens Protocols: Cultured breast cancer cells were harvested, and pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation Total RNA samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit. Protocol was modified based on the quality and quality of the samples. Customized adapters with unique molecular indexes (UMI) and Sample-specific dual-indexes primers were added to each library. The quantity of libraries was measured with Qubit and quality measured by TapStation Genomic DNA Assay. ENA-FIRST-PUBLIC 2022-07-05 ENA-LAST-UPDATE 2022-07-05 External Id SAMEA9424251 INSDC center alias Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC center name Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC first public 2022-07-05T12:03:08Z INSDC last update 2022-07-05T12:03:08Z INSDC status public Submitter Id E-MTAB-10733:MDA-MB-453_#1 broker name ArrayExpress cell_line MDAMB453 common name human developmental stage adult disease metastatic carcinoma growth condition in vitro immunophenotype ER negative isolate not applicable organism part mammary gland sample name E-MTAB-10733:MDA-MB-453_#1 ERS7147479 SAMEA9424252 9606 Homo sapiens Protocols: Cultured breast cancer cells were harvested, and pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation Total RNA samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit. Protocol was modified based on the quality and quality of the samples. Customized adapters with unique molecular indexes (UMI) and Sample-specific dual-indexes primers were added to each library. The quantity of libraries was measured with Qubit and quality measured by TapStation Genomic DNA Assay. ENA-FIRST-PUBLIC 2022-07-05 ENA-LAST-UPDATE 2022-07-05 External Id SAMEA9424252 INSDC center alias Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC center name Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC first public 2022-07-05T12:03:08Z INSDC last update 2022-07-05T12:03:08Z INSDC status public Submitter Id E-MTAB-10733:MDA-MB-453_#2 broker name ArrayExpress cell_line MDAMB453 common name human developmental stage adult disease metastatic carcinoma growth condition in vitro immunophenotype ER negative isolate not applicable organism part mammary gland sample name E-MTAB-10733:MDA-MB-453_#2 ERS7147480 SAMEA9424253 9606 Homo sapiens Protocols: Cultured breast cancer cells were harvested, and pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation Total RNA samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit. Protocol was modified based on the quality and quality of the samples. Customized adapters with unique molecular indexes (UMI) and Sample-specific dual-indexes primers were added to each library. The quantity of libraries was measured with Qubit and quality measured by TapStation Genomic DNA Assay. ENA-FIRST-PUBLIC 2022-07-05 ENA-LAST-UPDATE 2022-07-05 External Id SAMEA9424253 INSDC center alias Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC center name Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC first public 2022-07-05T12:03:08Z INSDC last update 2022-07-05T12:03:08Z INSDC status public Submitter Id E-MTAB-10733:PDX-ERa-negative_#1 broker name ArrayExpress common name human developmental stage adult disease breast cancer growth condition patient derived xenograft immunophenotype ER negative isolate not applicable organism part mammary gland sample name E-MTAB-10733:PDX-ERa-negative_#1 ERS7147481 SAMEA9424254 9606 Homo sapiens Protocols: Cultured breast cancer cells were harvested, and pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation Total RNA samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit. Protocol was modified based on the quality and quality of the samples. Customized adapters with unique molecular indexes (UMI) and Sample-specific dual-indexes primers were added to each library. The quantity of libraries was measured with Qubit and quality measured by TapStation Genomic DNA Assay. ENA-FIRST-PUBLIC 2022-07-05 ENA-LAST-UPDATE 2022-07-05 External Id SAMEA9424254 INSDC center alias Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC center name Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC first public 2022-07-05T12:03:08Z INSDC last update 2022-07-05T12:03:08Z INSDC status public Submitter Id E-MTAB-10733:PDX-ERa-negative_#2 broker name ArrayExpress common name human developmental stage adult disease breast cancer growth condition patient derived xenograft immunophenotype ER negative isolate not applicable organism part mammary gland sample name E-MTAB-10733:PDX-ERa-negative_#2 ERS7147482 SAMEA9424255 9606 Homo sapiens Protocols: Cultured breast cancer cells were harvested, and pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation Total RNA samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit. Protocol was modified based on the quality and quality of the samples. Customized adapters with unique molecular indexes (UMI) and Sample-specific dual-indexes primers were added to each library. The quantity of libraries was measured with Qubit and quality measured by TapStation Genomic DNA Assay. ENA-FIRST-PUBLIC 2022-07-05 ENA-LAST-UPDATE 2022-07-05 External Id SAMEA9424255 INSDC center alias Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC center name Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC first public 2022-07-05T12:03:08Z INSDC last update 2022-07-05T12:03:08Z INSDC status public Submitter Id E-MTAB-10733:MCF-7_#1 broker name ArrayExpress cell_line MCF-7 common name human developmental stage adult disease adenocarcinoma growth condition in vitro immunophenotype ER positive isolate not applicable organism part mammary gland sample name E-MTAB-10733:MCF-7_#1 ERS7147483 SAMEA9424256 9606 Homo sapiens Protocols: Cultured breast cancer cells were harvested, and pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation Total RNA samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit. Protocol was modified based on the quality and quality of the samples. Customized adapters with unique molecular indexes (UMI) and Sample-specific dual-indexes primers were added to each library. The quantity of libraries was measured with Qubit and quality measured by TapStation Genomic DNA Assay. ENA-FIRST-PUBLIC 2022-07-05 ENA-LAST-UPDATE 2022-07-05 External Id SAMEA9424256 INSDC center alias Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC center name Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC first public 2022-07-05T12:03:08Z INSDC last update 2022-07-05T12:03:08Z INSDC status public Submitter Id E-MTAB-10733:MCF-7_#2 broker name ArrayExpress cell_line MCF-7 common name human developmental stage adult disease adenocarcinoma growth condition in vitro immunophenotype ER positive isolate not applicable organism part mammary gland sample name E-MTAB-10733:MCF-7_#2 ERS7147484 SAMEA9424257 9606 Homo sapiens Protocols: Cultured breast cancer cells were harvested, and pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation Total RNA samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit. Protocol was modified based on the quality and quality of the samples. Customized adapters with unique molecular indexes (UMI) and Sample-specific dual-indexes primers were added to each library. The quantity of libraries was measured with Qubit and quality measured by TapStation Genomic DNA Assay. ENA-FIRST-PUBLIC 2022-07-05 ENA-LAST-UPDATE 2022-07-05 External Id SAMEA9424257 INSDC center alias Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC center name Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC first public 2022-07-05T12:03:08Z INSDC last update 2022-07-05T12:03:08Z INSDC status public Submitter Id E-MTAB-10733:PDX-ERa-positive_#1 broker name ArrayExpress common name human developmental stage adult disease breast cancer growth condition patient derived xenograft immunophenotype ER positive isolate not applicable organism part mammary gland sample name E-MTAB-10733:PDX-ERa-positive_#1 ERS7147485 SAMEA9424258 9606 Homo sapiens Protocols: Cultured breast cancer cells were harvested, and pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation Total RNA samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit. Protocol was modified based on the quality and quality of the samples. Customized adapters with unique molecular indexes (UMI) and Sample-specific dual-indexes primers were added to each library. The quantity of libraries was measured with Qubit and quality measured by TapStation Genomic DNA Assay. ENA-FIRST-PUBLIC 2022-07-05 ENA-LAST-UPDATE 2022-07-05 External Id SAMEA9424258 INSDC center alias Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC center name Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC first public 2022-07-05T12:03:08Z INSDC last update 2022-07-05T12:03:08Z INSDC status public Submitter Id E-MTAB-10733:PDX-ERa-positive_#2 broker name ArrayExpress common name human developmental stage adult disease breast cancer growth condition patient derived xenograft immunophenotype ER positive isolate not applicable organism part mammary gland sample name E-MTAB-10733:PDX-ERa-positive_#2 ERS7147486 SAMEA9424259 9606 Homo sapiens Protocols: Cultured breast cancer cells were harvested, and pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation Total RNA samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit. Protocol was modified based on the quality and quality of the samples. Customized adapters with unique molecular indexes (UMI) and Sample-specific dual-indexes primers were added to each library. The quantity of libraries was measured with Qubit and quality measured by TapStation Genomic DNA Assay. ENA-FIRST-PUBLIC 2022-07-05 ENA-LAST-UPDATE 2022-07-05 External Id SAMEA9424259 INSDC center alias Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC center name Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC first public 2022-07-05T12:03:08Z INSDC last update 2022-07-05T12:03:08Z INSDC status public Submitter Id E-MTAB-10733:T-47D_#1 broker name ArrayExpress cell_line T-47D common name human developmental stage adult disease breast ductal adenocarcinoma growth condition in vitro immunophenotype ER positive isolate not applicable organism part mammary gland sample name E-MTAB-10733:T-47D_#1 ERS7147487 SAMEA9424260 9606 Homo sapiens Protocols: Cultured breast cancer cells were harvested, and pellets were washed 3X with cold PBS solution before RNA extraction. Trizol / Chloroform extraction followed by isopropanol precipitation Total RNA samples (100 ng) were input in the RNA library construction using the KAPA RNA Hyper library prep kit. Protocol was modified based on the quality and quality of the samples. Customized adapters with unique molecular indexes (UMI) and Sample-specific dual-indexes primers were added to each library. The quantity of libraries was measured with Qubit and quality measured by TapStation Genomic DNA Assay. ENA-FIRST-PUBLIC 2022-07-05 ENA-LAST-UPDATE 2022-07-05 External Id SAMEA9424260 INSDC center alias Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC center name Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA. INSDC first public 2022-07-05T12:03:08Z INSDC last update 2022-07-05T12:03:08Z INSDC status public Submitter Id E-MTAB-10733:T-47D_#2 broker name ArrayExpress cell_line T-47D common name human developmental stage adult disease breast ductal adenocarcinoma growth condition in vitro immunophenotype ER positive isolate not applicable organism part mammary gland sample name E-MTAB-10733:T-47D_#2