ERX1200889 E-MTAB-4035:1810027O10Rik knockout abdominal muscle bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956445 SAMEA3649296 E-MTAB-4035:1810027O10Rik knockout abdominal muscle bioreplicate 1 1810027O10Rik knockout abdominal muscle bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 1810027O10Rik knockout genotype Experimental Factor: musculature organism part Experimental Factor: abdomen sampling site ERX1200890 E-MTAB-4035:1810027O10Rik knockout abdominal muscle bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956446 SAMEA3649297 E-MTAB-4035:1810027O10Rik knockout abdominal muscle bioreplicate 2 1810027O10Rik knockout abdominal muscle bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 1810027O10Rik knockout genotype Experimental Factor: musculature organism part Experimental Factor: abdomen sampling site ERX1200891 E-MTAB-4035:1810027O10Rik knockout abdominal muscle bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956447 SAMEA3649298 E-MTAB-4035:1810027O10Rik knockout abdominal muscle bioreplicate 3 1810027O10Rik knockout abdominal muscle bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 1810027O10Rik knockout genotype Experimental Factor: musculature organism part Experimental Factor: abdomen sampling site ERX1200892 E-MTAB-4035:1810027O10Rik knockout gonadal adipose bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956448 SAMEA3649299 E-MTAB-4035:1810027O10Rik knockout gonadal adipose bioreplicate 1 1810027O10Rik knockout gonadal adipose bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 1810027O10Rik knockout genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200893 E-MTAB-4035:1810027O10Rik knockout gonadal adipose bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956449 SAMEA3649300 E-MTAB-4035:1810027O10Rik knockout gonadal adipose bioreplicate 2 1810027O10Rik knockout gonadal adipose bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 1810027O10Rik knockout genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200894 E-MTAB-4035:1810027O10Rik knockout gonadal adipose bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956450 SAMEA3649301 E-MTAB-4035:1810027O10Rik knockout gonadal adipose bioreplicate 3 1810027O10Rik knockout gonadal adipose bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 1810027O10Rik knockout genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200895 E-MTAB-4035:1810027O10Rik knockout liver bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956451 SAMEA3649302 E-MTAB-4035:1810027O10Rik knockout liver bioreplicate 1 1810027O10Rik knockout liver bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 1810027O10Rik knockout genotype Experimental Factor: liver organism part ERX1200896 E-MTAB-4035:1810027O10Rik knockout liver bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956452 SAMEA3649303 E-MTAB-4035:1810027O10Rik knockout liver bioreplicate 2 1810027O10Rik knockout liver bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 1810027O10Rik knockout genotype Experimental Factor: liver organism part ERX1200897 E-MTAB-4035:1810027O10Rik knockout liver bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956453 SAMEA3649304 E-MTAB-4035:1810027O10Rik knockout liver bioreplicate 3 1810027O10Rik knockout liver bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 1810027O10Rik knockout genotype Experimental Factor: liver organism part ERX1200898 E-MTAB-4035:1810027O10Rik knockout lung bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956454 SAMEA3649305 E-MTAB-4035:1810027O10Rik knockout lung bioreplicate 1 1810027O10Rik knockout lung bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 1810027O10Rik knockout genotype Experimental Factor: lung organism part ERX1200899 E-MTAB-4035:1810027O10Rik knockout lung bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956455 SAMEA3649306 E-MTAB-4035:1810027O10Rik knockout lung bioreplicate 2 1810027O10Rik knockout lung bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 1810027O10Rik knockout genotype Experimental Factor: lung organism part ERX1200900 E-MTAB-4035:1810027O10Rik knockout lung bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956456 SAMEA3649307 E-MTAB-4035:1810027O10Rik knockout lung bioreplicate 3 1810027O10Rik knockout lung bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 1810027O10Rik knockout genotype Experimental Factor: lung organism part ERX1200901 E-MTAB-4035:3110043O21Rik knockout kidney bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956457 SAMEA3649308 E-MTAB-4035:3110043O21Rik knockout kidney bioreplicate 1 3110043O21Rik knockout kidney bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 3110043O21Rik knockout genotype Experimental Factor: kidney organism part ERX1200902 E-MTAB-4035:3110043O21Rik knockout kidney bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956458 SAMEA3649309 E-MTAB-4035:3110043O21Rik knockout kidney bioreplicate 2 3110043O21Rik knockout kidney bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 3110043O21Rik knockout genotype Experimental Factor: kidney organism part ERX1200903 E-MTAB-4035:3110043O21Rik knockout liver bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956459 SAMEA3649310 E-MTAB-4035:3110043O21Rik knockout liver bioreplicate 1 3110043O21Rik knockout liver bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 3110043O21Rik knockout genotype Experimental Factor: liver organism part ERX1200904 E-MTAB-4035:3110043O21Rik knockout liver bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956460 SAMEA3649311 E-MTAB-4035:3110043O21Rik knockout liver bioreplicate 2 3110043O21Rik knockout liver bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 3110043O21Rik knockout genotype Experimental Factor: liver organism part ERX1200905 E-MTAB-4035:3110043O21Rik knockout liver bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956461 SAMEA3649312 E-MTAB-4035:3110043O21Rik knockout liver bioreplicate 3 3110043O21Rik knockout liver bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 3110043O21Rik knockout genotype Experimental Factor: liver organism part ERX1200906 E-MTAB-4035:3110043O21Rik knockout spleen bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956462 SAMEA3649313 E-MTAB-4035:3110043O21Rik knockout spleen bioreplicate 1 3110043O21Rik knockout spleen bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 3110043O21Rik knockout genotype Experimental Factor: spleen organism part ERX1200907 E-MTAB-4035:3110043O21Rik knockout spleen bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956463 SAMEA3649314 E-MTAB-4035:3110043O21Rik knockout spleen bioreplicate 2 3110043O21Rik knockout spleen bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 3110043O21Rik knockout genotype Experimental Factor: spleen organism part ERX1200908 E-MTAB-4035:3110043O21Rik knockout spleen bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956464 SAMEA3649315 E-MTAB-4035:3110043O21Rik knockout spleen bioreplicate 3 3110043O21Rik knockout spleen bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: 3110043O21Rik knockout genotype Experimental Factor: spleen organism part ERX1200909 E-MTAB-4035:Sik1 knockout abdominal muscle bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956465 SAMEA3649316 E-MTAB-4035:Sik1 knockout abdominal muscle bioreplicate 1 Sik1 knockout abdominal muscle bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: Sik1 knockout genotype Experimental Factor: musculature organism part Experimental Factor: abdomen sampling site ERX1200910 E-MTAB-4035:Sik1 knockout abdominal muscle bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956466 SAMEA3649317 E-MTAB-4035:Sik1 knockout abdominal muscle bioreplicate 2 Sik1 knockout abdominal muscle bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: Sik1 knockout genotype Experimental Factor: musculature organism part Experimental Factor: abdomen sampling site ERX1200911 E-MTAB-4035:Sik1 knockout abdominal muscle bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956467 SAMEA3649318 E-MTAB-4035:Sik1 knockout abdominal muscle bioreplicate 3 Sik1 knockout abdominal muscle bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: Sik1 knockout genotype Experimental Factor: musculature organism part Experimental Factor: abdomen sampling site ERX1200912 E-MTAB-4035:Sik1 knockout gonadal adipose bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956468 SAMEA3649319 E-MTAB-4035:Sik1 knockout gonadal adipose bioreplicate 1 Sik1 knockout gonadal adipose bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: Sik1 knockout genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200913 E-MTAB-4035:Sik1 knockout gonadal adipose bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956469 SAMEA3649320 E-MTAB-4035:Sik1 knockout gonadal adipose bioreplicate 2 Sik1 knockout gonadal adipose bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: Sik1 knockout genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200914 E-MTAB-4035:Sik1 knockout gonadal adipose bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956470 SAMEA3649321 E-MTAB-4035:Sik1 knockout gonadal adipose bioreplicate 3 Sik1 knockout gonadal adipose bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: Sik1 knockout genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200915 E-MTAB-4035:Sik1 knockout liver bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956471 SAMEA3649322 E-MTAB-4035:Sik1 knockout liver bioreplicate 1 Sik1 knockout liver bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: Sik1 knockout genotype Experimental Factor: liver organism part ERX1200916 E-MTAB-4035:Sik1 knockout liver bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956472 SAMEA3649323 E-MTAB-4035:Sik1 knockout liver bioreplicate 2 Sik1 knockout liver bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: Sik1 knockout genotype Experimental Factor: liver organism part ERX1200917 E-MTAB-4035:Sik1 knockout liver bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956473 SAMEA3649324 E-MTAB-4035:Sik1 knockout liver bioreplicate 3 Sik1 knockout liver bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: Sik1 knockout genotype Experimental Factor: liver organism part ERX1200918 E-MTAB-4035:wild type genotype abdominal muscle bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956474 SAMEA3649325 E-MTAB-4035:wild type genotype abdominal muscle bioreplicate 1 wild type genotype abdominal muscle bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: musculature organism part Experimental Factor: abdomen sampling site ERX1200919 E-MTAB-4035:wild type genotype abdominal muscle bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956475 SAMEA3649326 E-MTAB-4035:wild type genotype abdominal muscle bioreplicate 2 wild type genotype abdominal muscle bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: musculature organism part Experimental Factor: abdomen sampling site ERX1200920 E-MTAB-4035:wild type genotype abdominal muscle bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956476 SAMEA3649327 E-MTAB-4035:wild type genotype abdominal muscle bioreplicate 3 wild type genotype abdominal muscle bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: musculature organism part Experimental Factor: abdomen sampling site ERX1200921 E-MTAB-4035:wild type genotype abdominal muscle bioreplicate 4 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956477 SAMEA3649328 E-MTAB-4035:wild type genotype abdominal muscle bioreplicate 4 wild type genotype abdominal muscle bioreplicate 4 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: musculature organism part Experimental Factor: abdomen sampling site ERX1200922 E-MTAB-4035:wild type genotype abdominal muscle bioreplicate 5 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956478 SAMEA3649329 E-MTAB-4035:wild type genotype abdominal muscle bioreplicate 5 wild type genotype abdominal muscle bioreplicate 5 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: musculature organism part Experimental Factor: abdomen sampling site ERX1200923 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956479 SAMEA3649330 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 1 wild type genotype gonadal adipose bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200924 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956480 SAMEA3649331 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 2 wild type genotype gonadal adipose bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200925 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956481 SAMEA3649332 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 3 wild type genotype gonadal adipose bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200926 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 4 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956482 SAMEA3649333 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 4 wild type genotype gonadal adipose bioreplicate 4 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200927 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 5 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956483 SAMEA3649334 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 5 wild type genotype gonadal adipose bioreplicate 5 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200928 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 6 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956484 SAMEA3649335 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 6 wild type genotype gonadal adipose bioreplicate 6 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200929 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 7 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956485 SAMEA3649336 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 7 wild type genotype gonadal adipose bioreplicate 7 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200930 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 8 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956486 SAMEA3649337 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 8 wild type genotype gonadal adipose bioreplicate 8 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200931 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 9 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956487 SAMEA3649338 E-MTAB-4035:wild type genotype gonadal adipose bioreplicate 9 wild type genotype gonadal adipose bioreplicate 9 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: adipose tissue organism part Experimental Factor: gonad sampling site ERX1200932 E-MTAB-4035:wild type genotype kidney bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956488 SAMEA3649339 E-MTAB-4035:wild type genotype kidney bioreplicate 1 wild type genotype kidney bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: kidney organism part ERX1200933 E-MTAB-4035:wild type genotype kidney bioreplicate 10 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956489 SAMEA3649340 E-MTAB-4035:wild type genotype kidney bioreplicate 10 wild type genotype kidney bioreplicate 10 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: kidney organism part ERX1200934 E-MTAB-4035:wild type genotype kidney bioreplicate 11 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956490 SAMEA3649341 E-MTAB-4035:wild type genotype kidney bioreplicate 11 wild type genotype kidney bioreplicate 11 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: kidney organism part ERX1200935 E-MTAB-4035:wild type genotype kidney bioreplicate 12 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956491 SAMEA3649342 E-MTAB-4035:wild type genotype kidney bioreplicate 12 wild type genotype kidney bioreplicate 12 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: kidney organism part ERX1200936 E-MTAB-4035:wild type genotype kidney bioreplicate 13 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956492 SAMEA3649343 E-MTAB-4035:wild type genotype kidney bioreplicate 13 wild type genotype kidney bioreplicate 13 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: kidney organism part ERX1200937 E-MTAB-4035:wild type genotype kidney bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956493 SAMEA3649344 E-MTAB-4035:wild type genotype kidney bioreplicate 2 wild type genotype kidney bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: kidney organism part ERX1200938 E-MTAB-4035:wild type genotype kidney bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956494 SAMEA3649345 E-MTAB-4035:wild type genotype kidney bioreplicate 3 wild type genotype kidney bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: kidney organism part ERX1200939 E-MTAB-4035:wild type genotype kidney bioreplicate 4 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956495 SAMEA3649346 E-MTAB-4035:wild type genotype kidney bioreplicate 4 wild type genotype kidney bioreplicate 4 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: kidney organism part ERX1200940 E-MTAB-4035:wild type genotype kidney bioreplicate 5 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956496 SAMEA3649347 E-MTAB-4035:wild type genotype kidney bioreplicate 5 wild type genotype kidney bioreplicate 5 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: kidney organism part ERX1200941 E-MTAB-4035:wild type genotype kidney bioreplicate 6 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956497 SAMEA3649348 E-MTAB-4035:wild type genotype kidney bioreplicate 6 wild type genotype kidney bioreplicate 6 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: kidney organism part ERX1200942 E-MTAB-4035:wild type genotype kidney bioreplicate 7 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956498 SAMEA3649349 E-MTAB-4035:wild type genotype kidney bioreplicate 7 wild type genotype kidney bioreplicate 7 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: kidney organism part ERX1200943 E-MTAB-4035:wild type genotype kidney bioreplicate 8 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956499 SAMEA3649350 E-MTAB-4035:wild type genotype kidney bioreplicate 8 wild type genotype kidney bioreplicate 8 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: kidney organism part ERX1200944 E-MTAB-4035:wild type genotype kidney bioreplicate 9 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956500 SAMEA3649351 E-MTAB-4035:wild type genotype kidney bioreplicate 9 wild type genotype kidney bioreplicate 9 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: kidney organism part ERX1200945 E-MTAB-4035:wild type genotype liver bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956501 SAMEA3649352 E-MTAB-4035:wild type genotype liver bioreplicate 1 wild type genotype liver bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200946 E-MTAB-4035:wild type genotype liver bioreplicate 10 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956502 SAMEA3649353 E-MTAB-4035:wild type genotype liver bioreplicate 10 wild type genotype liver bioreplicate 10 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200947 E-MTAB-4035:wild type genotype liver bioreplicate 11 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956503 SAMEA3649354 E-MTAB-4035:wild type genotype liver bioreplicate 11 wild type genotype liver bioreplicate 11 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200948 E-MTAB-4035:wild type genotype liver bioreplicate 12 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956504 SAMEA3649355 E-MTAB-4035:wild type genotype liver bioreplicate 12 wild type genotype liver bioreplicate 12 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200949 E-MTAB-4035:wild type genotype liver bioreplicate 13 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956505 SAMEA3649356 E-MTAB-4035:wild type genotype liver bioreplicate 13 wild type genotype liver bioreplicate 13 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200950 E-MTAB-4035:wild type genotype liver bioreplicate 14 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956506 SAMEA3649357 E-MTAB-4035:wild type genotype liver bioreplicate 14 wild type genotype liver bioreplicate 14 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200951 E-MTAB-4035:wild type genotype liver bioreplicate 15 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956507 SAMEA3649358 E-MTAB-4035:wild type genotype liver bioreplicate 15 wild type genotype liver bioreplicate 15 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200952 E-MTAB-4035:wild type genotype liver bioreplicate 16 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956508 SAMEA3649359 E-MTAB-4035:wild type genotype liver bioreplicate 16 wild type genotype liver bioreplicate 16 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200953 E-MTAB-4035:wild type genotype liver bioreplicate 17 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956509 SAMEA3649360 E-MTAB-4035:wild type genotype liver bioreplicate 17 wild type genotype liver bioreplicate 17 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200954 E-MTAB-4035:wild type genotype liver bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956510 SAMEA3649361 E-MTAB-4035:wild type genotype liver bioreplicate 2 wild type genotype liver bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200955 E-MTAB-4035:wild type genotype liver bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956511 SAMEA3649362 E-MTAB-4035:wild type genotype liver bioreplicate 3 wild type genotype liver bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200956 E-MTAB-4035:wild type genotype liver bioreplicate 4 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956512 SAMEA3649363 E-MTAB-4035:wild type genotype liver bioreplicate 4 wild type genotype liver bioreplicate 4 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200957 E-MTAB-4035:wild type genotype liver bioreplicate 5 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956513 SAMEA3649364 E-MTAB-4035:wild type genotype liver bioreplicate 5 wild type genotype liver bioreplicate 5 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200958 E-MTAB-4035:wild type genotype liver bioreplicate 6 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956514 SAMEA3649365 E-MTAB-4035:wild type genotype liver bioreplicate 6 wild type genotype liver bioreplicate 6 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200959 E-MTAB-4035:wild type genotype liver bioreplicate 7 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956515 SAMEA3649366 E-MTAB-4035:wild type genotype liver bioreplicate 7 wild type genotype liver bioreplicate 7 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200960 E-MTAB-4035:wild type genotype liver bioreplicate 8 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956516 SAMEA3649367 E-MTAB-4035:wild type genotype liver bioreplicate 8 wild type genotype liver bioreplicate 8 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200961 E-MTAB-4035:wild type genotype liver bioreplicate 9 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956517 SAMEA3649368 E-MTAB-4035:wild type genotype liver bioreplicate 9 wild type genotype liver bioreplicate 9 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: liver organism part ERX1200962 E-MTAB-4035:wild type genotype lung bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956518 SAMEA3649369 E-MTAB-4035:wild type genotype lung bioreplicate 1 wild type genotype lung bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: lung organism part ERX1200963 E-MTAB-4035:wild type genotype lung bioreplicate 10 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956519 SAMEA3649370 E-MTAB-4035:wild type genotype lung bioreplicate 10 wild type genotype lung bioreplicate 10 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: lung organism part ERX1200964 E-MTAB-4035:wild type genotype lung bioreplicate 11 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956520 SAMEA3649371 E-MTAB-4035:wild type genotype lung bioreplicate 11 wild type genotype lung bioreplicate 11 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: lung organism part ERX1200965 E-MTAB-4035:wild type genotype lung bioreplicate 12 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956521 SAMEA3649372 E-MTAB-4035:wild type genotype lung bioreplicate 12 wild type genotype lung bioreplicate 12 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: lung organism part ERX1200966 E-MTAB-4035:wild type genotype lung bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956522 SAMEA3649373 E-MTAB-4035:wild type genotype lung bioreplicate 2 wild type genotype lung bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: lung organism part ERX1200967 E-MTAB-4035:wild type genotype lung bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956523 SAMEA3649374 E-MTAB-4035:wild type genotype lung bioreplicate 3 wild type genotype lung bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: lung organism part ERX1200968 E-MTAB-4035:wild type genotype lung bioreplicate 4 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956524 SAMEA3649375 E-MTAB-4035:wild type genotype lung bioreplicate 4 wild type genotype lung bioreplicate 4 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: lung organism part ERX1200969 E-MTAB-4035:wild type genotype lung bioreplicate 5 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956525 SAMEA3649376 E-MTAB-4035:wild type genotype lung bioreplicate 5 wild type genotype lung bioreplicate 5 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: lung organism part ERX1200970 E-MTAB-4035:wild type genotype lung bioreplicate 6 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956526 SAMEA3649377 E-MTAB-4035:wild type genotype lung bioreplicate 6 wild type genotype lung bioreplicate 6 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: lung organism part ERX1200971 E-MTAB-4035:wild type genotype lung bioreplicate 7 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956527 SAMEA3649378 E-MTAB-4035:wild type genotype lung bioreplicate 7 wild type genotype lung bioreplicate 7 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: lung organism part ERX1200972 E-MTAB-4035:wild type genotype lung bioreplicate 8 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956528 SAMEA3649379 E-MTAB-4035:wild type genotype lung bioreplicate 8 wild type genotype lung bioreplicate 8 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: lung organism part ERX1200973 E-MTAB-4035:wild type genotype lung bioreplicate 9 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956529 SAMEA3649380 E-MTAB-4035:wild type genotype lung bioreplicate 9 wild type genotype lung bioreplicate 9 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: lung organism part ERX1200974 E-MTAB-4035:wild type genotype spleen bioreplicate 1 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956530 SAMEA3649381 E-MTAB-4035:wild type genotype spleen bioreplicate 1 wild type genotype spleen bioreplicate 1 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: spleen organism part ERX1200975 E-MTAB-4035:wild type genotype spleen bioreplicate 2 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956531 SAMEA3649382 E-MTAB-4035:wild type genotype spleen bioreplicate 2 wild type genotype spleen bioreplicate 2 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: spleen organism part ERX1200976 E-MTAB-4035:wild type genotype spleen bioreplicate 3 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956532 SAMEA3649383 E-MTAB-4035:wild type genotype spleen bioreplicate 3 wild type genotype spleen bioreplicate 3 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: spleen organism part ERX1200977 E-MTAB-4035:wild type genotype spleen bioreplicate 4 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956533 SAMEA3649384 E-MTAB-4035:wild type genotype spleen bioreplicate 4 wild type genotype spleen bioreplicate 4 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: spleen organism part ERX1200978 E-MTAB-4035:wild type genotype spleen bioreplicate 5 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956534 SAMEA3649385 E-MTAB-4035:wild type genotype spleen bioreplicate 5 wild type genotype spleen bioreplicate 5 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: spleen organism part ERX1200979 E-MTAB-4035:wild type genotype spleen bioreplicate 6 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956535 SAMEA3649386 E-MTAB-4035:wild type genotype spleen bioreplicate 6 wild type genotype spleen bioreplicate 6 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: spleen organism part ERX1200980 E-MTAB-4035:wild type genotype spleen bioreplicate 7 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956536 SAMEA3649387 E-MTAB-4035:wild type genotype spleen bioreplicate 7 wild type genotype spleen bioreplicate 7 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: spleen organism part ERX1200981 E-MTAB-4035:wild type genotype spleen bioreplicate 8 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956537 SAMEA3649388 E-MTAB-4035:wild type genotype spleen bioreplicate 8 wild type genotype spleen bioreplicate 8 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: spleen organism part ERX1200982 E-MTAB-4035:wild type genotype spleen bioreplicate 9 ERP013119 E-MTAB-4035 RNAseq of coding RNA in the liver, spleen, kidney, abdominal muscle and gonadal adipose tissue of knock out mice and wild type controls as part of the KOMP2 project. ERS956538 SAMEA3649389 E-MTAB-4035:wild type genotype spleen bioreplicate 9 wild type genotype spleen bioreplicate 9 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Knockout mice were generated on C57BL/6N background as part of the Knockout Mouse Project as described in Skarnes et al. 2011. Nature 474(7351): 337-42. doi: 10.1038/nature10163 (for more information see www.mousephenotype.org; www.komp.org; or www.kompphenotype.org). Mice were euthanised at 16 weeks of age and liver, spleen, kidney, abdominal muscle , or gonadal adipose tissue was dissected for RNA extraction. The strains used in this study were 1810027O10Rik , Sik1, 3110043O21Rik, and 3110043O21Rik knockout mice. RNA extraction was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Library preparation was performed as described in Wang L, Si Y, Dedow LK, Shao Y, Liu P, et al. (2011) A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand- Specific Multiplex RNA-Seq. PLoS ONE 6(10): e26426. doi:10.1371/journal.pone.0026426 Illumina HiSeq 2500 Experimental Factor: wild type genotype genotype Experimental Factor: spleen organism part