ERS1046950 SAMEA3859816 10090 Mus musculus Protocols: R1 and E14Tg2 mouse ESCs were cultured on 0,1% gelatin in ESC medium: knock-out DMEM supplemented with 15% FBS (Sigma) and 1,000 U/ml LIF ESGRO (Millipore). mESCs were treated at indicated concentrations to activate the Wnt pathway: purified Wnt3a (Peprotech); BIO (Calbiochem); CHIR99021 (Calbiochem). BIO and CHIR99021 were resuspendend in DMSO (Sigma). Briefly, ESCs were trypsinised and crosslinked in 1% formaldehyde for 10 min at room temperature. Crosslinking was quenched with 0.125 M glycine for 5 min. The pelleted cells were lysed in 1 ml ChIP buffer and sonicated for 10 min in a Bioruptor sonicator (Diagenode). The soluble material was quantified using Bradford assays. To immunoprecipitate the transcription factors, 500 g protein was used. Antibodies were incubated overnight with the chromatin. The immunocomplexes were recovered with 30 l protein A or G agarose bead slurries. The immunoprecipitated material was washed three times with low-salt buffer and one time with high-salt buffer. ENA first public 2017-03-14 ENA last update 2016-01-28 External Id SAMEA3859816 INSDC center alias Research Associate INSDC center name Research Associate INSDC first public 2017-03-14T17:01:19Z INSDC last update 2016-01-28T12:20:33Z INSDC status public Submitter Id E-MTAB-4358:TCF1 broker name ArrayExpress cell type embryonic stem cell common name house mouse sample name E-MTAB-4358:TCF1 ERS1046951 SAMEA3859817 10090 Mus musculus Protocols: R1 and E14Tg2 mouse ESCs were cultured on 0,1% gelatin in ESC medium: knock-out DMEM supplemented with 15% FBS (Sigma) and 1,000 U/ml LIF ESGRO (Millipore). mESCs were treated at indicated concentrations to activate the Wnt pathway: purified Wnt3a (Peprotech); BIO (Calbiochem); CHIR99021 (Calbiochem). BIO and CHIR99021 were resuspendend in DMSO (Sigma). Briefly, ESCs were trypsinised and crosslinked in 1% formaldehyde for 10 min at room temperature. Crosslinking was quenched with 0.125 M glycine for 5 min. The pelleted cells were lysed in 1 ml ChIP buffer and sonicated for 10 min in a Bioruptor sonicator (Diagenode). The soluble material was quantified using Bradford assays. To immunoprecipitate the transcription factors, 500 g protein was used. Antibodies were incubated overnight with the chromatin. The immunocomplexes were recovered with 30 l protein A or G agarose bead slurries. The immunoprecipitated material was washed three times with low-salt buffer and one time with high-salt buffer. ENA first public 2017-03-14 ENA last update 2016-01-28 External Id SAMEA3859817 INSDC center alias Research Associate INSDC center name Research Associate INSDC first public 2017-03-14T17:01:19Z INSDC last update 2016-01-28T12:20:33Z INSDC status public Submitter Id E-MTAB-4358:TCF1-IgG broker name ArrayExpress cell type embryonic stem cell common name house mouse sample name E-MTAB-4358:TCF1-IgG ERS1046952 SAMEA3859818 10090 Mus musculus Protocols: R1 and E14Tg2 mouse ESCs were cultured on 0,1% gelatin in ESC medium: knock-out DMEM supplemented with 15% FBS (Sigma) and 1,000 U/ml LIF ESGRO (Millipore). mESCs were treated at indicated concentrations to activate the Wnt pathway: purified Wnt3a (Peprotech); BIO (Calbiochem); CHIR99021 (Calbiochem). BIO and CHIR99021 were resuspendend in DMSO (Sigma). Briefly, ESCs were trypsinised and crosslinked in 1% formaldehyde for 10 min at room temperature. Crosslinking was quenched with 0.125 M glycine for 5 min. The pelleted cells were lysed in 1 ml ChIP buffer and sonicated for 10 min in a Bioruptor sonicator (Diagenode). The soluble material was quantified using Bradford assays. To immunoprecipitate the transcription factors, 500 g protein was used. Antibodies were incubated overnight with the chromatin. The immunocomplexes were recovered with 30 l protein A or G agarose bead slurries. The immunoprecipitated material was washed three times with low-salt buffer and one time with high-salt buffer. ENA first public 2017-03-14 ENA last update 2016-01-28 External Id SAMEA3859818 INSDC center alias Research Associate INSDC center name Research Associate INSDC first public 2017-03-14T17:01:19Z INSDC last update 2016-01-28T12:20:33Z INSDC status public Submitter Id E-MTAB-4358:TCF3 broker name ArrayExpress cell type embryonic stem cell common name house mouse sample name E-MTAB-4358:TCF3 ERS1046953 SAMEA3859819 10090 Mus musculus Protocols: R1 and E14Tg2 mouse ESCs were cultured on 0,1% gelatin in ESC medium: knock-out DMEM supplemented with 15% FBS (Sigma) and 1,000 U/ml LIF ESGRO (Millipore). mESCs were treated at indicated concentrations to activate the Wnt pathway: purified Wnt3a (Peprotech); BIO (Calbiochem); CHIR99021 (Calbiochem). BIO and CHIR99021 were resuspendend in DMSO (Sigma). Briefly, ESCs were trypsinised and crosslinked in 1% formaldehyde for 10 min at room temperature. Crosslinking was quenched with 0.125 M glycine for 5 min. The pelleted cells were lysed in 1 ml ChIP buffer and sonicated for 10 min in a Bioruptor sonicator (Diagenode). The soluble material was quantified using Bradford assays. To immunoprecipitate the transcription factors, 500 g protein was used. Antibodies were incubated overnight with the chromatin. The immunocomplexes were recovered with 30 l protein A or G agarose bead slurries. The immunoprecipitated material was washed three times with low-salt buffer and one time with high-salt buffer. ENA first public 2017-03-14 ENA last update 2016-01-28 External Id SAMEA3859819 INSDC center alias Research Associate INSDC center name Research Associate INSDC first public 2017-03-14T17:01:19Z INSDC last update 2016-01-28T12:20:33Z INSDC status public Submitter Id E-MTAB-4358:TCF3-IgG broker name ArrayExpress cell type embryonic stem cell common name house mouse sample name E-MTAB-4358:TCF3-IgG