ERS7421539 SAMEA9697305 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697305 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:FR-p84_c-cpe_1 age 10 breed pitbull terrier broker name ArrayExpress cell line TihoDProCarc0840 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genetic modification transfected with pFusionRed-C genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:FR-p84_c-cpe_1 sex castrated male stimulus treated with 20 microgram per milliliter C-CPE time 3 sub_species familiaris ERS7421540 SAMEA9697306 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697306 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:FR-p84_c-cpe_2 age 10 breed pitbull terrier broker name ArrayExpress cell line TihoDProCarc0840 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genetic modification transfected with pFusionRed-C genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:FR-p84_c-cpe_2 sex castrated male stimulus treated with 20 microgram per milliliter C-CPE time 3 sub_species familiaris ERS7421541 SAMEA9697307 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697307 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:FR-p84_c-cpe_3 age 10 breed pitbull terrier broker name ArrayExpress cell line TihoDProCarc0840 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genetic modification transfected with pFusionRed-C genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:FR-p84_c-cpe_3 sex castrated male stimulus treated with 20 microgram per milliliter C-CPE time 3 sub_species familiaris ERS7421542 SAMEA9697308 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697308 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:FR-p85_trans_1 age 10 breed pitbull terrier broker name ArrayExpress cell line TihoDProCarc0840 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genetic modification transfected with pFusionRed-C genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:FR-p85_trans_1 sex castrated male sub_species familiaris ERS7421543 SAMEA9697309 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697309 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:FR-p85_trans_2 age 10 breed pitbull terrier broker name ArrayExpress cell line TihoDProCarc0840 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genetic modification transfected with pFusionRed-C genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:FR-p85_trans_2 sex castrated male sub_species familiaris ERS7421544 SAMEA9697310 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697310 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:FR-p85_trans_3 age 10 breed pitbull terrier broker name ArrayExpress cell line TihoDProCarc0840 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genetic modification transfected with pFusionRed-C genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:FR-p85_trans_3 sex castrated male sub_species familiaris ERS7421545 SAMEA9697311 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697311 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:p286_c-cpe_1 age 10 breed pitbull terrier broker name ArrayExpress cell line TihoDProCarc0840 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:p286_c-cpe_1 sex castrated male stimulus treated with 20 microgram per milliliter C-CPE time 3 sub_species familiaris ERS7421546 SAMEA9697312 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697312 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:p286_c-cpe_2 age 10 breed pitbull terrier broker name ArrayExpress cell line TihoDProCarc0840 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:p286_c-cpe_2 sex castrated male stimulus treated with 20 microgram per milliliter C-CPE time 3 sub_species familiaris ERS7421547 SAMEA9697313 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697313 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:p286_c-cpe_3 age 10 breed pitbull terrier broker name ArrayExpress cell line TihoDProCarc0840 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:p286_c-cpe_3 sex castrated male stimulus treated with 20 microgram per milliliter C-CPE time 3 sub_species familiaris ERS7421548 SAMEA9697314 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697314 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:p287_con_1 age 10 breed pitbull terrier broker name ArrayExpress cell line TihoDProCarc0840 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:p287_con_1 sex castrated male sub_species familiaris ERS7421549 SAMEA9697315 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697315 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:p287_con_2 age 10 breed pitbull terrier broker name ArrayExpress cell line TihoDProCarc0840 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:p287_con_2 sex castrated male sub_species familiaris ERS7421550 SAMEA9697316 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697316 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:p287_con_3 age 10 breed pitbull terrier broker name ArrayExpress cell line TihoDProCarc0840 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:p287_con_3 sex castrated male sub_species familiaris ERS7421551 SAMEA9697317 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697317 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:FR-p68_c-cpe_1 age 6 breed german rough-haired pointer broker name ArrayExpress cell line TihoDProAdCarc0846 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genetic modification transfected with pFusionRed-C genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:FR-p68_c-cpe_1 sex male stimulus treated with 20 microgram per milliliter C-CPE time 3 sub_species familiaris ERS7421552 SAMEA9697318 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697318 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:FR-p68_c-cpe_2 age 6 breed german rough-haired pointer broker name ArrayExpress cell line TihoDProAdCarc0846 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genetic modification transfected with pFusionRed-C genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:FR-p68_c-cpe_2 sex male stimulus treated with 20 microgram per milliliter C-CPE time 3 sub_species familiaris ERS7421553 SAMEA9697319 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697319 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:FR-p68_c-cpe_3 age 6 breed german rough-haired pointer broker name ArrayExpress cell line TihoDProAdCarc0846 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genetic modification transfected with pFusionRed-C genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:FR-p68_c-cpe_3 sex male stimulus treated with 20 microgram per milliliter C-CPE time 3 sub_species familiaris ERS7421554 SAMEA9697320 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697320 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:FR-p69_trans_1 age 6 breed german rough-haired pointer broker name ArrayExpress cell line TihoDProAdCarc0846 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genetic modification transfected with pFusionRed-C genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:FR-p69_trans_1 sex male sub_species familiaris ERS7421555 SAMEA9697321 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697321 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:FR-p69_trans_2 age 6 breed german rough-haired pointer broker name ArrayExpress cell line TihoDProAdCarc0846 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genetic modification transfected with pFusionRed-C genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:FR-p69_trans_2 sex male sub_species familiaris ERS7421556 SAMEA9697322 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697322 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:FR-p69_trans_3 age 6 breed german rough-haired pointer broker name ArrayExpress cell line TihoDProAdCarc0846 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genetic modification transfected with pFusionRed-C genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:FR-p69_trans_3 sex male sub_species familiaris ERS7421557 SAMEA9697323 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697323 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:p539_c-cpe_1 age 6 breed german rough-haired pointer broker name ArrayExpress cell line TihoDProAdCarc0846 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:p539_c-cpe_1 sex male stimulus treated with 20 microgram per milliliter C-CPE time 3 sub_species familiaris ERS7421558 SAMEA9697324 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697324 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:p539_c-cpe_2 age 6 breed german rough-haired pointer broker name ArrayExpress cell line TihoDProAdCarc0846 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:p539_c-cpe_2 sex male stimulus treated with 20 microgram per milliliter C-CPE time 3 sub_species familiaris ERS7421559 SAMEA9697325 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697325 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:p539_c-cpe_3 age 6 breed german rough-haired pointer broker name ArrayExpress cell line TihoDProAdCarc0846 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:p539_c-cpe_3 sex male stimulus treated with 20 microgram per milliliter C-CPE time 3 sub_species familiaris ERS7421560 SAMEA9697326 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697326 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:p540_con_1 age 6 breed german rough-haired pointer broker name ArrayExpress cell line TihoDProAdCarc0846 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:p540_con_1 sex male sub_species familiaris ERS7421561 SAMEA9697327 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697327 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:p540_con_2 age 6 breed german rough-haired pointer broker name ArrayExpress cell line TihoDProAdCarc0846 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:p540_con_2 sex male sub_species familiaris ERS7421562 SAMEA9697328 9615 Canis lupus familiaris Protocols: Canine tumor cell lines TihoDProCarc0840 (0840) and TihoDProAdCarc0846 (0846) were derived from canine prostate carcinomas previously described by Reimann-Berg et. al (2011) and Packeiser et al. (2020). Both cell lines express CLDN3, -4 and -7 (Hammer et. al. 2016).Transfected cell lines 0840-FusionRed and 0846-FusionRed (transfected with pFusionRed-C (Cat.#FP411, Evrogen)) were generated and characterized. C-CPE was prepared as described previously by Becker et. al. 2019. Native and transfected cell lines were seeded in triplicate with a density of 5*105 cells in 6-well plates 48 hours to reach monolayer. C-CPE with a concentration of 20 µg/ml was added and the cells incubated for 3 hours to allow binding of C-CPE to CLDNs. In the next step, culture medium was removed, and cells were washed with 5ml phosphate buffered saline (PBS). TrypLETM Express (Gibco by Life technologiesTM, Darmstadt, Germany) was used for detaching cells, centrifugation at 1000 rpm for 10 min followed for pelleting. Pellets were stored at -80 °C and followed by RNA-isolation for transcriptome analysis. The cells were cultivated separately in 25 cm2 cell culture flasks in medium 199 (Gibco by Life Technologies, Darmstadt, Germany) supplemented with 10 % fetal calf serum (FCS Superior, Biochrom GmbH, Berlin, Germany) and 2 % penicillin/streptomycin (Biochrom GmbH, Berlin, Germany), and incubated in a humidified incubator maintained at 37 ˚C with 5 % CO2. Cultivation medium was replaced twice per week. Total RNA was isolated from transfected and native prostate tumor cell lines with C-CPE treatment and without C-CPE treatment using the RNeasy®Mini Kit RNA Purification (Qiagen, Hilden, Germany) according to the manufacturer´s instruction. RNA isolation was performed three times per cell line. On-column DNase digestion was carried out with RNase-Free DNase Set (Qiagen Hilden, Germany) to avoid genomic DNA contamination.The RNA quality was assessed using an Agilent RNA 6000 Nano kit and 2100 Bioanalyzer (Agilent). Samples with RNA integrity number (RIN) > 8 were used for the DNA library preparation using a TruSeq Stranded mRNA Sample Preparation kit according to the manufacture's protocol (Illumina). Briefly, 1 µg of total RNA was used as input for an mRNA enrichment using poly-T oligo coated magnetic beads and chemically fragmented under elevated temperature. The fragmented RNA was then reverse-transcribed into the first- and second-strand cDNA using random hexamers and Superscript II reverse transcriptase. Double stranded cDNA fragments were ligated with TruSeq RNA adapters with a unique DNA sequencing index and PCR-amplified. The DNA libraries were quality-controlled using an Agilent Technologies 2100 Bioanalyzer and Agilent DNA-1000 Chip kit. ENA first public 2021-11-30 ENA last update 2021-11-30 External Id SAMEA9697328 INSDC center alias Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC center name Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit INSDC first public 2021-11-30T12:27:11Z INSDC last update 2021-11-30T12:27:11Z INSDC status public Submitter Id E-MTAB-10839:p540_con_3 age 6 breed german rough-haired pointer broker name ArrayExpress cell line TihoDProAdCarc0846 cell type prostate gland cancer cell common name dog developmental stage adult disease prostate carcinoma genotype wild type genotype organism part prostate gland sample name E-MTAB-10839:p540_con_3 sex male sub_species familiaris