ERX6131382 E-MTAB-9532_2:Human_colon_16S8157837_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421563 SAMEA9697329 Human_colon_16S8157837_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part veniform appendix Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131383 E-MTAB-9532_2:Human_colon_16S8157873_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421564 SAMEA9697330 Human_colon_16S8157873_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part veniform appendix Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131384 E-MTAB-9532_2:Human_colon_16S8157897_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421565 SAMEA9697331 Human_colon_16S8157897_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part veniform appendix Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131385 E-MTAB-9532_2:Human_colon_16S8157832_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421566 SAMEA9697332 Human_colon_16S8157832_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ascending colon Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131386 E-MTAB-9532_2:Human_colon_16S8157898_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421567 SAMEA9697333 Human_colon_16S8157898_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ascending colon Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131387 E-MTAB-9532_2:Human_colon_16S8157899_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421568 SAMEA9697334 Human_colon_16S8157899_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ascending colon Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131388 E-MTAB-9532_2:Human_colon_16S8157831_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421569 SAMEA9697335 Human_colon_16S8157831_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part caecum Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131389 E-MTAB-9532_2:Human_colon_16S8157874_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421570 SAMEA9697336 Human_colon_16S8157874_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part caecum Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131390 E-MTAB-9532_2:Human_colon_16S8157888_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421571 SAMEA9697337 Human_colon_16S8157888_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part caecum Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131391 E-MTAB-9532_2:Human_colon_16S8157896_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421572 SAMEA9697338 Human_colon_16S8157896_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part caecum Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131392 E-MTAB-9532_2:Human_colon_16S8157834_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421573 SAMEA9697339 Human_colon_16S8157834_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part descending colon Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131393 E-MTAB-9532_2:Human_colon_16S8157900_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421574 SAMEA9697340 Human_colon_16S8157900_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part descending colon Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131394 E-MTAB-9532_2:Human_colon_16S8157839_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421575 SAMEA9697341 Human_colon_16S8157839_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part duodenum Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131395 E-MTAB-9532_2:Human_colon_16S8157841_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421576 SAMEA9697342 Human_colon_16S8157841_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ileum Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131396 E-MTAB-9532_2:Human_colon_16S8157842_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421577 SAMEA9697343 Human_colon_16S8157842_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ileum Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131397 E-MTAB-9532_2:Human_colon_16S8157844_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421578 SAMEA9697344 Human_colon_16S8157844_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ileum Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131398 E-MTAB-9532_2:Human_colon_16S8157845_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421579 SAMEA9697345 Human_colon_16S8157845_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ileum Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131399 E-MTAB-9532_2:Human_colon_16S8157840_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421580 SAMEA9697346 Human_colon_16S8157840_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part jejunum Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131400 E-MTAB-9532_2:Human_colon_16S8157895_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421581 SAMEA9697347 Human_colon_16S8157895_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part jejunum Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131401 E-MTAB-9532_2:Human_colon_16S8157875_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421582 SAMEA9697348 Human_colon_16S8157875_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part mesenteric lymph node Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131402 E-MTAB-9532_2:Human_colon_16S8157887_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421583 SAMEA9697349 Human_colon_16S8157887_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part mesenteric lymph node Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131403 E-MTAB-9532_2:Human_colon_16S8157836_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421584 SAMEA9697350 Human_colon_16S8157836_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part rectum Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131404 E-MTAB-9532_2:Human_colon_16S8157835_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421585 SAMEA9697351 Human_colon_16S8157835_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part sigmoid colon Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131405 E-MTAB-9532_2:Human_colon_16S8157843_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421586 SAMEA9697352 Human_colon_16S8157843_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part sigmoid colon Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131406 E-MTAB-9532_2:Human_colon_16S8157877_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421587 SAMEA9697353 Human_colon_16S8157877_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part sigmoid colon Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131407 E-MTAB-9532_2:Human_colon_16S8157833_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421588 SAMEA9697354 Human_colon_16S8157833_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part transverse colon Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131408 E-MTAB-9532_2:Human_colon_16S8157872_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421589 SAMEA9697355 Human_colon_16S8157872_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part transverse colon Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131409 E-MTAB-9532_2:Human_colon_16S8157876_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421590 SAMEA9697356 Human_colon_16S8157876_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part transverse colon Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131410 E-MTAB-9532_2:Human_colon_16S8157901_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421591 SAMEA9697357 Human_colon_16S8157901_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part transverse colon Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131411 E-MTAB-9532_2:Human_colon_16S8157902_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421592 SAMEA9697358 Human_colon_16S8157902_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part transverse colon Experimental Factor: cell type B cell Experimental Factor: protocol 10x Ig enrichment ERX6131412 E-MTAB-9532_2:Human_colon_16S8157821_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421593 SAMEA9697359 Human_colon_16S8157821_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part veniform appendix Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131413 E-MTAB-9532_2:Human_colon_16S8157857_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421594 SAMEA9697360 Human_colon_16S8157857_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part veniform appendix Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131414 E-MTAB-9532_2:Human_colon_16S8157881_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421595 SAMEA9697361 Human_colon_16S8157881_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part veniform appendix Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131415 E-MTAB-9532_2:Human_colon_16S8157816_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421596 SAMEA9697362 Human_colon_16S8157816_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ascending colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131416 E-MTAB-9532_2:Human_colon_16S8157882_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421597 SAMEA9697363 Human_colon_16S8157882_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ascending colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131417 E-MTAB-9532_2:Human_colon_16S8157883_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421598 SAMEA9697364 Human_colon_16S8157883_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ascending colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131418 E-MTAB-9532_2:Human_colon_16S8117833_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421599 SAMEA9697365 Human_colon_16S8117833_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part caecum Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131419 E-MTAB-9532_2:Human_colon_16S8157815_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421600 SAMEA9697366 Human_colon_16S8157815_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part caecum Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131420 E-MTAB-9532_2:Human_colon_16S8157858_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421601 SAMEA9697367 Human_colon_16S8157858_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part caecum Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131421 E-MTAB-9532_2:Human_colon_16S8157861_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421602 SAMEA9697368 Human_colon_16S8157861_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part caecum Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131422 E-MTAB-9532_2:Human_colon_16S8157880_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421603 SAMEA9697369 Human_colon_16S8157880_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part caecum Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131423 E-MTAB-9532_2:Human_colon_16S8157818_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421604 SAMEA9697370 Human_colon_16S8157818_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part descending colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131424 E-MTAB-9532_2:Human_colon_16S8157884_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421605 SAMEA9697371 Human_colon_16S8157884_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part descending colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131425 E-MTAB-9532_2:Human_colon_16S8157823_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421606 SAMEA9697372 Human_colon_16S8157823_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part duodenum Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131426 E-MTAB-9532_2:Human_colon_16S8157825_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421607 SAMEA9697373 Human_colon_16S8157825_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ileum Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131427 E-MTAB-9532_2:Human_colon_16S8157826_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421608 SAMEA9697374 Human_colon_16S8157826_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ileum Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131428 E-MTAB-9532_2:Human_colon_16S8157828_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421609 SAMEA9697375 Human_colon_16S8157828_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ileum Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131429 E-MTAB-9532_2:Human_colon_16S8157829_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421610 SAMEA9697376 Human_colon_16S8157829_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part ileum Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131430 E-MTAB-9532_2:Human_colon_16S8157824_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421611 SAMEA9697377 Human_colon_16S8157824_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part jejunum Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131431 E-MTAB-9532_2:Human_colon_16S8157879_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421612 SAMEA9697378 Human_colon_16S8157879_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part jejunum Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131432 E-MTAB-9532_2:Human_colon_16S8117832_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421613 SAMEA9697379 Human_colon_16S8117832_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part mesenteric lymph node Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131433 E-MTAB-9532_2:Human_colon_16S8157859_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421614 SAMEA9697380 Human_colon_16S8157859_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part mesenteric lymph node Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131434 E-MTAB-9532_2:Human_colon_16S8157820_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421615 SAMEA9697381 Human_colon_16S8157820_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part rectum Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131435 E-MTAB-9532_2:Human_colon_16S8117835_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421616 SAMEA9697382 Human_colon_16S8117835_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part sigmoid colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131436 E-MTAB-9532_2:Human_colon_16S8157819_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421617 SAMEA9697383 Human_colon_16S8157819_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part sigmoid colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131437 E-MTAB-9532_2:Human_colon_16S8157827_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421618 SAMEA9697384 Human_colon_16S8157827_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part sigmoid colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131438 E-MTAB-9532_2:Human_colon_16S8117834_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421619 SAMEA9697385 Human_colon_16S8117834_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part transverse colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131439 E-MTAB-9532_2:Human_colon_16S8157817_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421620 SAMEA9697386 Human_colon_16S8157817_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part transverse colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131440 E-MTAB-9532_2:Human_colon_16S8157856_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421621 SAMEA9697387 Human_colon_16S8157856_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part transverse colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131441 E-MTAB-9532_2:Human_colon_16S8157860_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421622 SAMEA9697388 Human_colon_16S8157860_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part transverse colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131442 E-MTAB-9532_2:Human_colon_16S8157885_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421623 SAMEA9697389 Human_colon_16S8157885_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part transverse colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment ERX6131443 E-MTAB-9532_2:Human_colon_16S8157886_p ERP131268 PRJEB47029 The human gastrointestinal tract through space and time- Adult VDJ ERS7421624 SAMEA9697390 Human_colon_16S8157886_p RNA-Seq TRANSCRIPTOMIC SINGLE CELL PCR For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol. Illumina HiSeq 4000 Experimental Factor: organism part transverse colon Experimental Factor: cell type T cell Experimental Factor: protocol 10x TCR enrichment