ERX6201846 E-MTAB-10899:B67 Control 1_s ERP131496 PRJEB47232 RNA-sequencing of human adherent glioblastoma stem cell line following RNA interference targeting TRIM26 ERS7494921 SAMEA9816293 B67 Control 1_s RNA-Seq TRANSCRIPTOMIC RANDOM B67 GSCs were infected with MISSION TRC lentiviruses with shRNA targeting human TRIM26 (sh4050, sh4051) or with control (sh002) and 5 days later harvested for total RNA Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Ribosomal RNA was removed by a hybridization method using Ribo-ZERO kits (Illumina). mRNA was fragmented and reverse transcribed to yield double-stranded cDNA. cDNA was blunt ended, had an A base added to the 3′ ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12 cycles using primers incorporating unique index tags. Illumina HiSeq 2500 Experimental Factor: genetic modification scrambled siRNA ERX6201847 E-MTAB-10899:B67 Control 2_s ERP131496 PRJEB47232 RNA-sequencing of human adherent glioblastoma stem cell line following RNA interference targeting TRIM26 ERS7494922 SAMEA9816294 B67 Control 2_s RNA-Seq TRANSCRIPTOMIC RANDOM B67 GSCs were infected with MISSION TRC lentiviruses with shRNA targeting human TRIM26 (sh4050, sh4051) or with control (sh002) and 5 days later harvested for total RNA Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Ribosomal RNA was removed by a hybridization method using Ribo-ZERO kits (Illumina). mRNA was fragmented and reverse transcribed to yield double-stranded cDNA. cDNA was blunt ended, had an A base added to the 3′ ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12 cycles using primers incorporating unique index tags. Illumina HiSeq 2500 Experimental Factor: genetic modification scrambled siRNA ERX6201848 E-MTAB-10899:B67 Control 3_s ERP131496 PRJEB47232 RNA-sequencing of human adherent glioblastoma stem cell line following RNA interference targeting TRIM26 ERS7494923 SAMEA9816295 B67 Control 3_s RNA-Seq TRANSCRIPTOMIC RANDOM B67 GSCs were infected with MISSION TRC lentiviruses with shRNA targeting human TRIM26 (sh4050, sh4051) or with control (sh002) and 5 days later harvested for total RNA Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Ribosomal RNA was removed by a hybridization method using Ribo-ZERO kits (Illumina). mRNA was fragmented and reverse transcribed to yield double-stranded cDNA. cDNA was blunt ended, had an A base added to the 3′ ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12 cycles using primers incorporating unique index tags. Illumina HiSeq 2500 Experimental Factor: genetic modification scrambled siRNA ERX6201849 E-MTAB-10899:B67 TRIM26i.1 1_s ERP131496 PRJEB47232 RNA-sequencing of human adherent glioblastoma stem cell line following RNA interference targeting TRIM26 ERS7494924 SAMEA9816296 B67 TRIM26i.1 1_s RNA-Seq TRANSCRIPTOMIC RANDOM B67 GSCs were infected with MISSION TRC lentiviruses with shRNA targeting human TRIM26 (sh4050, sh4051) or with control (sh002) and 5 days later harvested for total RNA Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Ribosomal RNA was removed by a hybridization method using Ribo-ZERO kits (Illumina). mRNA was fragmented and reverse transcribed to yield double-stranded cDNA. cDNA was blunt ended, had an A base added to the 3′ ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12 cycles using primers incorporating unique index tags. Illumina HiSeq 2500 Experimental Factor: genetic modification TRIM26 knockdown by siRNA 1 ERX6201850 E-MTAB-10899:B67 TRIM26i.1 2_s ERP131496 PRJEB47232 RNA-sequencing of human adherent glioblastoma stem cell line following RNA interference targeting TRIM26 ERS7494925 SAMEA9816297 B67 TRIM26i.1 2_s RNA-Seq TRANSCRIPTOMIC RANDOM B67 GSCs were infected with MISSION TRC lentiviruses with shRNA targeting human TRIM26 (sh4050, sh4051) or with control (sh002) and 5 days later harvested for total RNA Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Ribosomal RNA was removed by a hybridization method using Ribo-ZERO kits (Illumina). mRNA was fragmented and reverse transcribed to yield double-stranded cDNA. cDNA was blunt ended, had an A base added to the 3′ ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12 cycles using primers incorporating unique index tags. Illumina HiSeq 2500 Experimental Factor: genetic modification TRIM26 knockdown by siRNA 1 ERX6201851 E-MTAB-10899:B67 TRIM26i.1 3_s ERP131496 PRJEB47232 RNA-sequencing of human adherent glioblastoma stem cell line following RNA interference targeting TRIM26 ERS7494926 SAMEA9816298 B67 TRIM26i.1 3_s RNA-Seq TRANSCRIPTOMIC RANDOM B67 GSCs were infected with MISSION TRC lentiviruses with shRNA targeting human TRIM26 (sh4050, sh4051) or with control (sh002) and 5 days later harvested for total RNA Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Ribosomal RNA was removed by a hybridization method using Ribo-ZERO kits (Illumina). mRNA was fragmented and reverse transcribed to yield double-stranded cDNA. cDNA was blunt ended, had an A base added to the 3′ ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12 cycles using primers incorporating unique index tags. Illumina HiSeq 2500 Experimental Factor: genetic modification TRIM26 knockdown by siRNA 1 ERX6201852 E-MTAB-10899:B67 TRIM26i.2 1_s ERP131496 PRJEB47232 RNA-sequencing of human adherent glioblastoma stem cell line following RNA interference targeting TRIM26 ERS7494927 SAMEA9816299 B67 TRIM26i.2 1_s RNA-Seq TRANSCRIPTOMIC RANDOM B67 GSCs were infected with MISSION TRC lentiviruses with shRNA targeting human TRIM26 (sh4050, sh4051) or with control (sh002) and 5 days later harvested for total RNA Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Ribosomal RNA was removed by a hybridization method using Ribo-ZERO kits (Illumina). mRNA was fragmented and reverse transcribed to yield double-stranded cDNA. cDNA was blunt ended, had an A base added to the 3′ ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12 cycles using primers incorporating unique index tags. Illumina HiSeq 2500 Experimental Factor: genetic modification TRIM26 knockdown by siRNA 2 ERX6201853 E-MTAB-10899:B67 TRIM26i.2 2_s ERP131496 PRJEB47232 RNA-sequencing of human adherent glioblastoma stem cell line following RNA interference targeting TRIM26 ERS7494928 SAMEA9816300 B67 TRIM26i.2 2_s RNA-Seq TRANSCRIPTOMIC RANDOM B67 GSCs were infected with MISSION TRC lentiviruses with shRNA targeting human TRIM26 (sh4050, sh4051) or with control (sh002) and 5 days later harvested for total RNA Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Ribosomal RNA was removed by a hybridization method using Ribo-ZERO kits (Illumina). mRNA was fragmented and reverse transcribed to yield double-stranded cDNA. cDNA was blunt ended, had an A base added to the 3′ ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12 cycles using primers incorporating unique index tags. Illumina HiSeq 2500 Experimental Factor: genetic modification TRIM26 knockdown by siRNA 2 ERX6201854 E-MTAB-10899:B67 TRIM26i.2 3_s ERP131496 PRJEB47232 RNA-sequencing of human adherent glioblastoma stem cell line following RNA interference targeting TRIM26 ERS7494929 SAMEA9816301 B67 TRIM26i.2 3_s RNA-Seq TRANSCRIPTOMIC RANDOM B67 GSCs were infected with MISSION TRC lentiviruses with shRNA targeting human TRIM26 (sh4050, sh4051) or with control (sh002) and 5 days later harvested for total RNA Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). Ribosomal RNA was removed by a hybridization method using Ribo-ZERO kits (Illumina). mRNA was fragmented and reverse transcribed to yield double-stranded cDNA. cDNA was blunt ended, had an A base added to the 3′ ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12 cycles using primers incorporating unique index tags. Illumina HiSeq 2500 Experimental Factor: genetic modification TRIM26 knockdown by siRNA 2