ERX1477268 E-MTAB-4729:A549 BR1 ERP015518 RNA-seq analysis of two cell line models of lung diseases in Project 3 of Open Targets - Epigenomes of Cell Lines ERS1146905 SAMEA3959771 A549 BR1 RNA-Seq TRANSCRIPTOMIC cDNA F12K + 10% FBS Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer’s protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: A549 cell line ERX1477269 E-MTAB-4729:A549 BR2 ERP015518 RNA-seq analysis of two cell line models of lung diseases in Project 3 of Open Targets - Epigenomes of Cell Lines ERS1146906 SAMEA3959772 A549 BR2 RNA-Seq TRANSCRIPTOMIC cDNA F12K + 10% FBS Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer’s protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: A549 cell line ERX1477270 E-MTAB-4729:BEAS-2B BR1 ERP015518 RNA-seq analysis of two cell line models of lung diseases in Project 3 of Open Targets - Epigenomes of Cell Lines ERS1146907 SAMEA3959773 BEAS-2B BR1 RNA-Seq TRANSCRIPTOMIC cDNA Lonza BEBM with additive kit (Cat# CC-3170, GA-1000 additive omitted). Plates coated with 0.01 mg/ml fibronectin, 0.03 mg/ml bovine collagen type I and 0.01 mg/mL BSA dissolved in BEBM Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer’s protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: BEAS-2B cell line ERX1477271 E-MTAB-4729:BEAS-2B BR2 ERP015518 RNA-seq analysis of two cell line models of lung diseases in Project 3 of Open Targets - Epigenomes of Cell Lines ERS1146908 SAMEA3959774 BEAS-2B BR2 RNA-Seq TRANSCRIPTOMIC cDNA Lonza BEBM with additive kit (Cat# CC-3170, GA-1000 additive omitted). Plates coated with 0.01 mg/ml fibronectin, 0.03 mg/ml bovine collagen type I and 0.01 mg/mL BSA dissolved in BEBM Qiagen RNeasy plus mini kit Total RNA from each sample was used as input for the Illumina TruSeq Stranded messenger RNA LT Sample Prep Kit (Illumina) and sequencing libraries were created according to the manufacturer’s protocol. Briefly, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Following purification, the mRNA was fragmented and copied into first strand complementary DNA using random primers and reverse transcriptase. Second strand cDNA synthesis was then done using DNA polymerase I, RNase H and substituting dUTP for dTTP. The cDNA was ligated to adapters and enriched with PCR to create the final cDNA library. The library was pooled and sequenced 150 0 F Application Read Forward 1 1 R Application Read Reverse 76 Illumina HiSeq 2500 Experimental Factor: BEAS-2B cell line