<?xml version="1.0" encoding="UTF-8"?>
<STUDY_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <STUDY accession="ERP131812" alias="ena-STUDY-STATION BIOLOGIQUE DE ROSCOFF-14-09-2021-15:41:27:586-1719" center_name="STATION BIOLOGIQUE DE ROSCOFF">
    <IDENTIFIERS>
      <PRIMARY_ID>ERP131812</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioProject">PRJEB47533</EXTERNAL_ID>
      <SUBMITTER_ID namespace="STATION BIOLOGIQUE DE ROSCOFF">ena-STUDY-STATION BIOLOGIQUE DE ROSCOFF-14-09-2021-15:41:27:586-1719</SUBMITTER_ID>
    </IDENTIFIERS>
    <DESCRIPTOR>
      <STUDY_TITLE>RAD sequencing for the hydrothermal vent gastropod Ifremeria nautilei</STUDY_TITLE>
      <STUDY_TYPE existing_study_type="Other"/>
      <STUDY_ABSTRACT>This study contains RAD-sequencing data generated for the hydrothermal vent gastropod Ifremeria nautilei. RAD-sequencing libraries were constructed at the Station Biologique de Roscoff, France, in the ANR CERBERUS project. Individual ddRAD libraries were constructed according to the protocol available in dx.doi.org/10.17504/protocols.io.bv4tn8wn. Briefly, genomic DNA from each samples were extracted and quantified, then double-digested with two restriction enzymes, ligated to adaptors, and PCR-amplified to yield Illumina compatible fragments. All individuals were then pooled equally in a single library that was then size-selected and subjected to quality control before Illumina HiSeq4000 PE150 sequencing at the Genoscope, in the eDNAbyss umbrella project.</STUDY_ABSTRACT>
      <STUDY_DESCRIPTION>This study contains RAD-sequencing data generated for the hydrothermal vent gastropod Ifremeria nautilei. RAD-sequencing libraries were constructed at the Station Biologique de Roscoff, France, in the ANR CERBERUS project. Individual ddRAD libraries were constructed according to the protocol available in dx.doi.org/10.17504/protocols.io.bv4tn8wn. Briefly, genomic DNA from each samples were extracted and quantified, then double-digested with two restriction enzymes, ligated to adaptors, and PCR-amplified to yield Illumina compatible fragments. All individuals were then pooled equally in a single library that was then size-selected and subjected to quality control before Illumina HiSeq4000 PE150 sequencing at the Genoscope, in the eDNAbyss umbrella project.</STUDY_DESCRIPTION>
    </DESCRIPTOR>
    <STUDY_ATTRIBUTES>
      <STUDY_ATTRIBUTE>
        <TAG>ENA-FIRST-PUBLIC</TAG>
        <VALUE>2022-01-05</VALUE>
      </STUDY_ATTRIBUTE>
      <STUDY_ATTRIBUTE>
        <TAG>ENA-LAST-UPDATE</TAG>
        <VALUE>2022-02-11</VALUE>
      </STUDY_ATTRIBUTE>
    </STUDY_ATTRIBUTES>
  </STUDY>
</STUDY_SET>
