ERX6349179 E-MTAB-10929:129T2 E12.5 replicate 1_p ERP131818 PRJEB47538 RNA-seq of dissected cardiac septa from a mouse developmental time course ERS7740576 SAMEA10083770 129T2 E12.5 replicate 1_p RNA-Seq TRANSCRIPTOMIC Inverse rRNA Embryos were dissected from the uterus in ice-cold PBS under a dissecting scope (Leica MZ8) using micro-tweezers. Once the heart was harvested from the embryo and the pericardial membrane was removed, the atrial and ventricular regions were separated by splitting the heart into 2 halves through the atrioventricular canal using micro-dissection tungsten needles. Then, the auricular appendages were cut off the atrial tissue and the remaining mesenchymal tissue from the atrioventricular canal was further trimmed. The remaining tissue, containing the atrial septum, was stored at -80ºC in RNAlater solution (Ambion) for further analysis. Total RNA was purified using miRNeasy Kit (Qiagen). RNA libraries were made from 300-400ng of total RNA using TruSeq (ribozero) stranded RNA Library Preparation Kit following the manufacturers protocol (Illumina). Illumina HiSeq 2500 Experimental Factor: strain 129T2/SvEms Experimental Factor: developmental stage embryonic day 12.5 ERX6349180 E-MTAB-10929:129T2 E12.5 replicate 2_p ERP131818 PRJEB47538 RNA-seq of dissected cardiac septa from a mouse developmental time course ERS7740577 SAMEA10083772 129T2 E12.5 replicate 2_p RNA-Seq TRANSCRIPTOMIC Inverse rRNA Embryos were dissected from the uterus in ice-cold PBS under a dissecting scope (Leica MZ8) using micro-tweezers. Once the heart was harvested from the embryo and the pericardial membrane was removed, the atrial and ventricular regions were separated by splitting the heart into 2 halves through the atrioventricular canal using micro-dissection tungsten needles. Then, the auricular appendages were cut off the atrial tissue and the remaining mesenchymal tissue from the atrioventricular canal was further trimmed. The remaining tissue, containing the atrial septum, was stored at -80ºC in RNAlater solution (Ambion) for further analysis. Total RNA was purified using miRNeasy Kit (Qiagen). RNA libraries were made from 300-400ng of total RNA using TruSeq (ribozero) stranded RNA Library Preparation Kit following the manufacturers protocol (Illumina). Illumina HiSeq 2500 Experimental Factor: strain 129T2/SvEms Experimental Factor: developmental stage embryonic day 12.5 ERX6349181 E-MTAB-10929:129T2 E14.5 replicate 1_p ERP131818 PRJEB47538 RNA-seq of dissected cardiac septa from a mouse developmental time course ERS7740578 SAMEA10083774 129T2 E14.5 replicate 1_p RNA-Seq TRANSCRIPTOMIC Inverse rRNA Embryos were dissected from the uterus in ice-cold PBS under a dissecting scope (Leica MZ8) using micro-tweezers. Once the heart was harvested from the embryo and the pericardial membrane was removed, the atrial and ventricular regions were separated by splitting the heart into 2 halves through the atrioventricular canal using micro-dissection tungsten needles. Then, the auricular appendages were cut off the atrial tissue and the remaining mesenchymal tissue from the atrioventricular canal was further trimmed. The remaining tissue, containing the atrial septum, was stored at -80ºC in RNAlater solution (Ambion) for further analysis. Total RNA was purified using miRNeasy Kit (Qiagen). RNA libraries were made from 300-400ng of total RNA using TruSeq (ribozero) stranded RNA Library Preparation Kit following the manufacturers protocol (Illumina). Illumina HiSeq 2500 Experimental Factor: strain 129T2/SvEms Experimental Factor: developmental stage embryonic day 14.5 ERX6349182 E-MTAB-10929:129T2 E14.5 replicate 2_p ERP131818 PRJEB47538 RNA-seq of dissected cardiac septa from a mouse developmental time course ERS7740579 SAMEA10083776 129T2 E14.5 replicate 2_p RNA-Seq TRANSCRIPTOMIC Inverse rRNA Embryos were dissected from the uterus in ice-cold PBS under a dissecting scope (Leica MZ8) using micro-tweezers. Once the heart was harvested from the embryo and the pericardial membrane was removed, the atrial and ventricular regions were separated by splitting the heart into 2 halves through the atrioventricular canal using micro-dissection tungsten needles. Then, the auricular appendages were cut off the atrial tissue and the remaining mesenchymal tissue from the atrioventricular canal was further trimmed. The remaining tissue, containing the atrial septum, was stored at -80ºC in RNAlater solution (Ambion) for further analysis. Total RNA was purified using miRNeasy Kit (Qiagen). RNA libraries were made from 300-400ng of total RNA using TruSeq (ribozero) stranded RNA Library Preparation Kit following the manufacturers protocol (Illumina). Illumina HiSeq 2500 Experimental Factor: strain 129T2/SvEms Experimental Factor: developmental stage embryonic day 14.5 ERX6349183 E-MTAB-10929:129T2 E16.5 replicate 1_p ERP131818 PRJEB47538 RNA-seq of dissected cardiac septa from a mouse developmental time course ERS7740580 SAMEA10083777 129T2 E16.5 replicate 1_p RNA-Seq TRANSCRIPTOMIC Inverse rRNA Embryos were dissected from the uterus in ice-cold PBS under a dissecting scope (Leica MZ8) using micro-tweezers. Once the heart was harvested from the embryo and the pericardial membrane was removed, the atrial and ventricular regions were separated by splitting the heart into 2 halves through the atrioventricular canal using micro-dissection tungsten needles. Then, the auricular appendages were cut off the atrial tissue and the remaining mesenchymal tissue from the atrioventricular canal was further trimmed. The remaining tissue, containing the atrial septum, was stored at -80ºC in RNAlater solution (Ambion) for further analysis. Total RNA was purified using miRNeasy Kit (Qiagen). RNA libraries were made from 300-400ng of total RNA using TruSeq (ribozero) stranded RNA Library Preparation Kit following the manufacturers protocol (Illumina). Illumina HiSeq 2500 Experimental Factor: strain 129T2/SvEms Experimental Factor: developmental stage embryonic day 16.5 ERX6349184 E-MTAB-10929:129T2 E16.5 replicate 2_p ERP131818 PRJEB47538 RNA-seq of dissected cardiac septa from a mouse developmental time course ERS7740581 SAMEA10083779 129T2 E16.5 replicate 2_p RNA-Seq TRANSCRIPTOMIC Inverse rRNA Embryos were dissected from the uterus in ice-cold PBS under a dissecting scope (Leica MZ8) using micro-tweezers. Once the heart was harvested from the embryo and the pericardial membrane was removed, the atrial and ventricular regions were separated by splitting the heart into 2 halves through the atrioventricular canal using micro-dissection tungsten needles. Then, the auricular appendages were cut off the atrial tissue and the remaining mesenchymal tissue from the atrioventricular canal was further trimmed. The remaining tissue, containing the atrial septum, was stored at -80ºC in RNAlater solution (Ambion) for further analysis. Total RNA was purified using miRNeasy Kit (Qiagen). RNA libraries were made from 300-400ng of total RNA using TruSeq (ribozero) stranded RNA Library Preparation Kit following the manufacturers protocol (Illumina). Illumina HiSeq 2500 Experimental Factor: strain 129T2/SvEms Experimental Factor: developmental stage embryonic day 16.5 ERX6349185 E-MTAB-10929:QSi5 E12.5 replicate 1_p ERP131818 PRJEB47538 RNA-seq of dissected cardiac septa from a mouse developmental time course ERS7740582 SAMEA10083781 QSi5 E12.5 replicate 1_p RNA-Seq TRANSCRIPTOMIC Inverse rRNA Embryos were dissected from the uterus in ice-cold PBS under a dissecting scope (Leica MZ8) using micro-tweezers. Once the heart was harvested from the embryo and the pericardial membrane was removed, the atrial and ventricular regions were separated by splitting the heart into 2 halves through the atrioventricular canal using micro-dissection tungsten needles. Then, the auricular appendages were cut off the atrial tissue and the remaining mesenchymal tissue from the atrioventricular canal was further trimmed. The remaining tissue, containing the atrial septum, was stored at -80ºC in RNAlater solution (Ambion) for further analysis. Total RNA was purified using miRNeasy Kit (Qiagen). RNA libraries were made from 300-400ng of total RNA using TruSeq (ribozero) stranded RNA Library Preparation Kit following the manufacturers protocol (Illumina). Illumina HiSeq 2500 Experimental Factor: strain QSi5 Experimental Factor: developmental stage embryonic day 12.5 ERX6349186 E-MTAB-10929:QSi5 E12.5 replicate 2_p ERP131818 PRJEB47538 RNA-seq of dissected cardiac septa from a mouse developmental time course ERS7740583 SAMEA10083783 QSi5 E12.5 replicate 2_p RNA-Seq TRANSCRIPTOMIC Inverse rRNA Embryos were dissected from the uterus in ice-cold PBS under a dissecting scope (Leica MZ8) using micro-tweezers. Once the heart was harvested from the embryo and the pericardial membrane was removed, the atrial and ventricular regions were separated by splitting the heart into 2 halves through the atrioventricular canal using micro-dissection tungsten needles. Then, the auricular appendages were cut off the atrial tissue and the remaining mesenchymal tissue from the atrioventricular canal was further trimmed. The remaining tissue, containing the atrial septum, was stored at -80ºC in RNAlater solution (Ambion) for further analysis. Total RNA was purified using miRNeasy Kit (Qiagen). RNA libraries were made from 300-400ng of total RNA using TruSeq (ribozero) stranded RNA Library Preparation Kit following the manufacturers protocol (Illumina). Illumina HiSeq 2500 Experimental Factor: strain QSi5 Experimental Factor: developmental stage embryonic day 12.5 ERX6349187 E-MTAB-10929:QSi5 E14.5 replicate 1_p ERP131818 PRJEB47538 RNA-seq of dissected cardiac septa from a mouse developmental time course ERS7740584 SAMEA10083785 QSi5 E14.5 replicate 1_p RNA-Seq TRANSCRIPTOMIC Inverse rRNA Embryos were dissected from the uterus in ice-cold PBS under a dissecting scope (Leica MZ8) using micro-tweezers. Once the heart was harvested from the embryo and the pericardial membrane was removed, the atrial and ventricular regions were separated by splitting the heart into 2 halves through the atrioventricular canal using micro-dissection tungsten needles. Then, the auricular appendages were cut off the atrial tissue and the remaining mesenchymal tissue from the atrioventricular canal was further trimmed. The remaining tissue, containing the atrial septum, was stored at -80ºC in RNAlater solution (Ambion) for further analysis. Total RNA was purified using miRNeasy Kit (Qiagen). RNA libraries were made from 300-400ng of total RNA using TruSeq (ribozero) stranded RNA Library Preparation Kit following the manufacturers protocol (Illumina). Illumina HiSeq 2500 Experimental Factor: strain QSi5 Experimental Factor: developmental stage embryonic day 14.5 ERX6349188 E-MTAB-10929:QSi5 E14.5 replicate 2_p ERP131818 PRJEB47538 RNA-seq of dissected cardiac septa from a mouse developmental time course ERS7740585 SAMEA10083787 QSi5 E14.5 replicate 2_p RNA-Seq TRANSCRIPTOMIC Inverse rRNA Embryos were dissected from the uterus in ice-cold PBS under a dissecting scope (Leica MZ8) using micro-tweezers. Once the heart was harvested from the embryo and the pericardial membrane was removed, the atrial and ventricular regions were separated by splitting the heart into 2 halves through the atrioventricular canal using micro-dissection tungsten needles. Then, the auricular appendages were cut off the atrial tissue and the remaining mesenchymal tissue from the atrioventricular canal was further trimmed. The remaining tissue, containing the atrial septum, was stored at -80ºC in RNAlater solution (Ambion) for further analysis. Total RNA was purified using miRNeasy Kit (Qiagen). RNA libraries were made from 300-400ng of total RNA using TruSeq (ribozero) stranded RNA Library Preparation Kit following the manufacturers protocol (Illumina). Illumina HiSeq 2500 Experimental Factor: strain QSi5 Experimental Factor: developmental stage embryonic day 14.5 ERX6349189 E-MTAB-10929:QSi5 E16.5 replicate 1_p ERP131818 PRJEB47538 RNA-seq of dissected cardiac septa from a mouse developmental time course ERS7740586 SAMEA10083789 QSi5 E16.5 replicate 1_p RNA-Seq TRANSCRIPTOMIC Inverse rRNA Embryos were dissected from the uterus in ice-cold PBS under a dissecting scope (Leica MZ8) using micro-tweezers. Once the heart was harvested from the embryo and the pericardial membrane was removed, the atrial and ventricular regions were separated by splitting the heart into 2 halves through the atrioventricular canal using micro-dissection tungsten needles. Then, the auricular appendages were cut off the atrial tissue and the remaining mesenchymal tissue from the atrioventricular canal was further trimmed. The remaining tissue, containing the atrial septum, was stored at -80ºC in RNAlater solution (Ambion) for further analysis. Total RNA was purified using miRNeasy Kit (Qiagen). RNA libraries were made from 300-400ng of total RNA using TruSeq (ribozero) stranded RNA Library Preparation Kit following the manufacturers protocol (Illumina). Illumina HiSeq 2500 Experimental Factor: strain QSi5 Experimental Factor: developmental stage embryonic day 16.5 ERX6349190 E-MTAB-10929:QSi5 E16.5 replicate 2_p ERP131818 PRJEB47538 RNA-seq of dissected cardiac septa from a mouse developmental time course ERS7740587 SAMEA10083791 QSi5 E16.5 replicate 2_p RNA-Seq TRANSCRIPTOMIC Inverse rRNA Embryos were dissected from the uterus in ice-cold PBS under a dissecting scope (Leica MZ8) using micro-tweezers. Once the heart was harvested from the embryo and the pericardial membrane was removed, the atrial and ventricular regions were separated by splitting the heart into 2 halves through the atrioventricular canal using micro-dissection tungsten needles. Then, the auricular appendages were cut off the atrial tissue and the remaining mesenchymal tissue from the atrioventricular canal was further trimmed. The remaining tissue, containing the atrial septum, was stored at -80ºC in RNAlater solution (Ambion) for further analysis. Total RNA was purified using miRNeasy Kit (Qiagen). RNA libraries were made from 300-400ng of total RNA using TruSeq (ribozero) stranded RNA Library Preparation Kit following the manufacturers protocol (Illumina). Illumina HiSeq 2500 Experimental Factor: strain QSi5 Experimental Factor: developmental stage embryonic day 16.5