ERS1157792 SAMEA3986682 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986682 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:24Z INSDC status public Submitter Id E-MTAB-2758:Sample 1 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 1 ERS1157793 SAMEA3986683 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986683 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:25Z INSDC status public Submitter Id E-MTAB-2758:Sample 2 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 2 ERS1157794 SAMEA3986684 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986684 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:25Z INSDC status public Submitter Id E-MTAB-2758:Sample 3 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 3 ERS1157795 SAMEA3986685 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986685 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:26Z INSDC status public Submitter Id E-MTAB-2758:Sample 4 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 4 ERS1157796 SAMEA3986686 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986686 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:26Z INSDC status public Submitter Id E-MTAB-2758:Sample 5 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 5 ERS1157797 SAMEA3986687 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986687 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:26Z INSDC status public Submitter Id E-MTAB-2758:Sample 6 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 6 ERS1157798 SAMEA3986688 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986688 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:26Z INSDC status public Submitter Id E-MTAB-2758:Sample 7 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 7 ERS1157799 SAMEA3986689 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986689 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:27Z INSDC status public Submitter Id E-MTAB-2758:Sample 8 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 8 ERS1157800 SAMEA3986690 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986690 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:27Z INSDC status public Submitter Id E-MTAB-2758:Sample 9 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 9 ERS1157801 SAMEA3986691 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986691 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:27Z INSDC status public Submitter Id E-MTAB-2758:Sample 10 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 10 ERS1157802 SAMEA3986692 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986692 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:27Z INSDC status public Submitter Id E-MTAB-2758:Sample 11 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 11 ERS1157803 SAMEA3986693 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986693 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:28Z INSDC status public Submitter Id E-MTAB-2758:Sample 12 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 12 ERS1157804 SAMEA3986694 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986694 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:28Z INSDC status public Submitter Id E-MTAB-2758:Sample 13 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 13 ERS1157805 SAMEA3986695 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986695 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:28Z INSDC status public Submitter Id E-MTAB-2758:Sample 14 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 14 ERS1157807 SAMEA3986697 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986697 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:29Z INSDC status public Submitter Id E-MTAB-2758:Sample 16 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 16 ERS1157808 SAMEA3986698 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986698 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:29Z INSDC status public Submitter Id E-MTAB-2758:Sample 17 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 17 ERS1157809 SAMEA3986699 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986699 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:30Z INSDC status public Submitter Id E-MTAB-2758:Sample 18 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 18 ERS1157810 SAMEA3986700 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986700 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:30Z INSDC status public Submitter Id E-MTAB-2758:Sample 19 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 19 ERS1157811 SAMEA3986701 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986701 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:30Z INSDC status public Submitter Id E-MTAB-2758:Sample 20 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 20 ERS1157812 SAMEA3986702 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986702 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:30Z INSDC status public Submitter Id E-MTAB-2758:Sample 21 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 21 ERS1157813 SAMEA3986703 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986703 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:31Z INSDC status public Submitter Id E-MTAB-2758:Sample 22 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 22 ERS1157814 SAMEA3986704 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986704 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:31Z INSDC status public Submitter Id E-MTAB-2758:Sample 23 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 23 ERS1157815 SAMEA3986705 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986705 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:32Z INSDC status public Submitter Id E-MTAB-2758:Sample 24 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 24 ERS1157816 SAMEA3986706 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986706 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:32Z INSDC status public Submitter Id E-MTAB-2758:Sample 25 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 25 ERS1157817 SAMEA3986707 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986707 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:32Z INSDC status public Submitter Id E-MTAB-2758:Sample 26 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 26 ERS1157818 SAMEA3986708 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986708 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:32Z INSDC status public Submitter Id E-MTAB-2758:Sample 27 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 27 ERS1157819 SAMEA3986709 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986709 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:33Z INSDC status public Submitter Id E-MTAB-2758:Sample 28 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 28 ERS1157820 SAMEA3986710 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986710 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:33Z INSDC status public Submitter Id E-MTAB-2758:Sample 29 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 29 ERS1157821 SAMEA3986711 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986711 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:33Z INSDC status public Submitter Id E-MTAB-2758:Sample 30 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 30 ERS1157806 SAMEA3986696 9606 Homo sapiens Protocols: A2780 ovarian cancer cells were obtained from ECACC (European Collection of Animal Cell Culture) and grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum, 1% (v/v) 2mL L-glutamine and 1% (v/v) penicillin (10 k units/mL)/streptomycin (10 mg/mL), at 310 K in a humidified atmosphere containing 5% CO2. Maintenance passages were done at ca. 80% confluency. Cells were seeded at 3 × 10^6 in P100 petri dishes and left to incubate for 24 h at 310 K in a humidified atmosphere containing 5% CO2. Stock solutions of the negative control and compound 1 were prepared in 5% DMSO, 10% saline, and 85% RPMI-1640 medium. Cells were exposed to compound 1 at a final concentration of 0.15 μM. After 4, 12, 24 and 48 h exposure, medium was removed from triplicate compound-exposed and control-exposed dishes, and cells were washed twice with PBS and collected using trypsin. Between 5 × 10^6 and 1 × 10^7 cells in each sample were taken forward for RNA extraction. Using QIAshredder spin columns (Qiagen) the cells were lysed, as per manufacturers instructions. DNA was eliminated from each sample using gDNA spin columns, and the RNA was purified using RNeasy spin columns and buffer solutions (RNeasy plus mini kit, QIagen) as per the manufacturers instructions. RNA was collected in 70 μL RNase-free water and stored at -70 C. Libraries were prepped using Truseq by the Oxford Genomics Centre, UK ENA first public 2016-05-12 ENA last update 2016-05-12 External Id SAMEA3986696 INSDC center alias Warwick University INSDC center name Warwick University INSDC first public 2016-05-12T17:02:43Z INSDC last update 2016-05-12T13:59:29Z INSDC status public Submitter Id E-MTAB-2758:Sample 15 broker name ArrayExpress cell line A2780 cell type ovarian cancer cell lines common name human sample name E-MTAB-2758:Sample 15