ERP132001 PRJEB47700 E-MTAB-10981 Biosafety circuits for Mycoplasma - DNA-seq for passages 2, 3 and 15 of clonal Mycoplasma pneumoniae biosafety strains to study long-term stability of kill-switch devices We have engineered synthetic gene switches to control and limit Mycoplasma growth for biosafety containment applications. Mycoplasmas have high mutation rates and, the accumulation of mutations that inactivate the circuit is expected. However, the question is how resilient is the kill-switch to mutation and whether it is more sensitive to the accumulation of mutations. Therefore, we did the whole-genome sequencing of the three Mycoplasma biosafety strains, designed in our study, at different passages (p2, p3 and p15) or after IPTG-treatment at passage 3 (p3IPTG) We have engineered synthetic gene switches to control and limit Mycoplasma growth for biosafety containment applications. Mycoplasmas have high mutation rates and, the accumulation of mutations that inactivate the circuit is expected. However, the question is how resilient is the kill-switch to mutation and whether it is more sensitive to the accumulation of mutations. Therefore, we did the whole-genome sequencing of the three Mycoplasma biosafety strains, designed in our study, at different passages (p2, p3 and p15) or after IPTG-treatment at passage 3 (p3IPTG) E-MTAB-10981 in ArrayExpress http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-10981 ENA-FIRST-PUBLIC 2022-02-09 ENA-LAST-UPDATE 2022-02-09