ERX6434103 E-MTAB-10989:Sample 1_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825594 SAMEA10169122 Sample 1_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference empty vector Experimental Factor: stimulus IFNg Experimental Factor: time 6 ERX6434104 E-MTAB-10989:Sample 10_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825595 SAMEA10169123 Sample 10_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference empty vector Experimental Factor: stimulus pIC Experimental Factor: time 6 ERX6434105 E-MTAB-10989:Sample 11_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825596 SAMEA10169124 Sample 11_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference NCoR1 siRNA Experimental Factor: stimulus pIC Experimental Factor: time 6 ERX6434106 E-MTAB-10989:Sample 12_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825597 SAMEA10169125 Sample 12_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference NCoR1 siRNA Experimental Factor: stimulus pIC Experimental Factor: time 6 ERX6434107 E-MTAB-10989:Sample 13_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825598 SAMEA10169126 Sample 13_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference empty vector Experimental Factor: stimulus CpG and pIC and IFNg Experimental Factor: time 6 ERX6434108 E-MTAB-10989:Sample 14_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825599 SAMEA10169127 Sample 14_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference empty vector Experimental Factor: stimulus CpG and pIC and IFNg Experimental Factor: time 6 ERX6434109 E-MTAB-10989:Sample 15_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825600 SAMEA10169128 Sample 15_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference NCoR1 siRNA Experimental Factor: stimulus CpG and pIC and IFNg Experimental Factor: time 6 ERX6434110 E-MTAB-10989:Sample 16_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825601 SAMEA10169129 Sample 16_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference NCoR1 siRNA Experimental Factor: stimulus CpG and pIC and IFNg Experimental Factor: time 6 ERX6434111 E-MTAB-10989:Sample 2_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825602 SAMEA10169130 Sample 2_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference empty vector Experimental Factor: stimulus IFNg Experimental Factor: time 6 ERX6434112 E-MTAB-10989:Sample 3_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825603 SAMEA10169131 Sample 3_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference NCoR1 siRNA Experimental Factor: stimulus IFNg Experimental Factor: time 6 ERX6434113 E-MTAB-10989:Sample 4_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825604 SAMEA10169132 Sample 4_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference NCoR1 siRNA Experimental Factor: stimulus IFNg Experimental Factor: time 6 ERX6434114 E-MTAB-10989:Sample 5_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825605 SAMEA10169133 Sample 5_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference empty vector Experimental Factor: stimulus pIC Experimental Factor: time 2 ERX6434115 E-MTAB-10989:Sample 6_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825606 SAMEA10169134 Sample 6_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference empty vector Experimental Factor: stimulus pIC Experimental Factor: time 2 ERX6434116 E-MTAB-10989:Sample 7_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825607 SAMEA10169135 Sample 7_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference NCoR1 siRNA Experimental Factor: stimulus pIC Experimental Factor: time 2 ERX6434117 E-MTAB-10989:Sample 8_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825608 SAMEA10169136 Sample 8_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference NCoR1 siRNA Experimental Factor: stimulus pIC Experimental Factor: time 2 ERX6434118 E-MTAB-10989:Sample 9_p ERP132013 PRJEB47711 RNA-seq of Control and NCoR1 KD CD8α+ DCs at 0hr and 6hr of stimulation ERS7825609 SAMEA10169137 Sample 9_p RNA-Seq TRANSCRIPTOMIC PolyA DC Cell lines (CD8+ cDC1 control and NCoR1 KD) were grown in IMDM medium supplemented with 10% FBS and antibiotic solution, 5 x 10-5 M b-mercaptoethanol, sodium bicarbonate and HEPES. The cells were maintained at 37°C in a humidified incubator with 5% CO2. These DCs were dissociated with a short incubation of 2-3 min in a nonenzymatic, 5 mM EDTA in 20 mM HEPES buffer. Cells were plated in 6-well plates at a density of 5 x 105 cells/ml overnight. The cells were then challenged with different activation media containing IFNg, TLR3 agonist pIC, and CpG+pIC+IFNg for 2hr or 6hr. For performing RT-qPCR analysis the cells were washed in the plate once with PBS followed by addition of RNA-later (RLT) buffer (Qiagen) for lysis of cells. The plates were then stored at -80oC until further RNA isolation and processing of samples. mRNA was isolated from 2ug of total RNA using magnetic beads mRNA isolation kit (PolyA mRNA isolation Module, NEB) RNA-seq library preparation was performed using mRNA library preparation kit (NEB) NextSeq 550 Experimental Factor: RNA interference empty vector Experimental Factor: stimulus pIC Experimental Factor: time 6