ERX6625717 E-MTAB-11053:Sample 1_p ERP132462 PRJEB48141 RNA-seq of human HT-29 WT and SMYD2-KO xenograft implanted into the flanks of Rag1−/− mice ERS8032921 SAMEA10378347 Sample 1_p RNA-Seq TRANSCRIPTOMIC Oligo-dT 2 × 10^6 HT-29 cells were implanted into the flanks of Rag1−/− mice via subcutaneous injection. Tumor tissues were collected 20 day post injection. Total RNA was extracted using peqGOLD RNA Kit (732-2868, Peqlab) according to the manufacturer's protocol. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. PCR was performed with Phusion High-Fidelity DNA polymerase (New England Biolabs), Universal PCR primers and Index (X) Primer. PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. Illumina HiSeq 1500 Experimental Factor: cell type wild type genotype ERX6625718 E-MTAB-11053:Sample 2_p ERP132462 PRJEB48141 RNA-seq of human HT-29 WT and SMYD2-KO xenograft implanted into the flanks of Rag1−/− mice ERS8032922 SAMEA10378348 Sample 2_p RNA-Seq TRANSCRIPTOMIC Oligo-dT 2 × 10^6 HT-29 cells were implanted into the flanks of Rag1−/− mice via subcutaneous injection. Tumor tissues were collected 20 day post injection. Total RNA was extracted using peqGOLD RNA Kit (732-2868, Peqlab) according to the manufacturer's protocol. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. PCR was performed with Phusion High-Fidelity DNA polymerase (New England Biolabs), Universal PCR primers and Index (X) Primer. PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. Illumina HiSeq 1500 Experimental Factor: cell type wild type genotype ERX6625719 E-MTAB-11053:Sample 3_p ERP132462 PRJEB48141 RNA-seq of human HT-29 WT and SMYD2-KO xenograft implanted into the flanks of Rag1−/− mice ERS8032923 SAMEA10378349 Sample 3_p RNA-Seq TRANSCRIPTOMIC Oligo-dT 2 × 10^6 HT-29 cells were implanted into the flanks of Rag1−/− mice via subcutaneous injection. Tumor tissues were collected 20 day post injection. Total RNA was extracted using peqGOLD RNA Kit (732-2868, Peqlab) according to the manufacturer's protocol. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. PCR was performed with Phusion High-Fidelity DNA polymerase (New England Biolabs), Universal PCR primers and Index (X) Primer. PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. Illumina HiSeq 1500 Experimental Factor: cell type SMYD2-KO2 ERX6625720 E-MTAB-11053:Sample 4_p ERP132462 PRJEB48141 RNA-seq of human HT-29 WT and SMYD2-KO xenograft implanted into the flanks of Rag1−/− mice ERS8032924 SAMEA10378350 Sample 4_p RNA-Seq TRANSCRIPTOMIC Oligo-dT 2 × 10^6 HT-29 cells were implanted into the flanks of Rag1−/− mice via subcutaneous injection. Tumor tissues were collected 20 day post injection. Total RNA was extracted using peqGOLD RNA Kit (732-2868, Peqlab) according to the manufacturer's protocol. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. PCR was performed with Phusion High-Fidelity DNA polymerase (New England Biolabs), Universal PCR primers and Index (X) Primer. PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. Illumina HiSeq 1500 Experimental Factor: cell type SMYD2-KO2 ERX6625721 E-MTAB-11053:Sample 5_p ERP132462 PRJEB48141 RNA-seq of human HT-29 WT and SMYD2-KO xenograft implanted into the flanks of Rag1−/− mice ERS8032925 SAMEA10378351 Sample 5_p RNA-Seq TRANSCRIPTOMIC Oligo-dT 2 × 10^6 HT-29 cells were implanted into the flanks of Rag1−/− mice via subcutaneous injection. Tumor tissues were collected 20 day post injection. Total RNA was extracted using peqGOLD RNA Kit (732-2868, Peqlab) according to the manufacturer's protocol. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. PCR was performed with Phusion High-Fidelity DNA polymerase (New England Biolabs), Universal PCR primers and Index (X) Primer. PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. Illumina HiSeq 1500 Experimental Factor: cell type SMYD2-KO6 ERX6625722 E-MTAB-11053:Sample 6_p ERP132462 PRJEB48141 RNA-seq of human HT-29 WT and SMYD2-KO xenograft implanted into the flanks of Rag1−/− mice ERS8032926 SAMEA10378352 Sample 6_p RNA-Seq TRANSCRIPTOMIC Oligo-dT 2 × 10^6 HT-29 cells were implanted into the flanks of Rag1−/− mice via subcutaneous injection. Tumor tissues were collected 20 day post injection. Total RNA was extracted using peqGOLD RNA Kit (732-2868, Peqlab) according to the manufacturer's protocol. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample. PCR was performed with Phusion High-Fidelity DNA polymerase (New England Biolabs), Universal PCR primers and Index (X) Primer. PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. Illumina HiSeq 1500 Experimental Factor: cell type SMYD2-KO6