ERS1237087 SAMEA4065977 410658 soil metagenome Soil from Colmar, sampled one year after organic waste product apllication ENA first public 2017-06-01 ENA last update 2016-10-21 External Id SAMEA4065977 INSDC center alias INRA-CMSE INSDC center name UMR Agroecology, INRA - Burgundy University, Dijon, France INSDC first public 2017-06-01T17:02:03Z INSDC last update 2016-10-21T09:34:42Z INSDC status public Submitter Id B4T2_18S city Colmar collection date 2012-03 common name SOERE_PRO_2012 depth 0.2 environment (biome) cropland biome environment (feature) microbial feature environment (material) arable soil geographic location (country and/or sea) France geographic location (elevation) 175 geographic location (latitude) 48.0833 geographic location (longitude) 7.3667 geographic location (region and locality) Alsace history/previous land use 12 years of organic waste application (every 2 years) horizon A horizon investigation type metagenome multiplex identifiers CACGTAGATC nucleic acid amplification 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation: 94degC_3min; 35 cycles of: denaturation: 94degC_1min; annealing: 52degC_1min; elongation: 72degC_1min, final elongation: 72degC_5min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5 position to specifically identify each sample and avoid PCR biases. nucleic acid extraction DNA was extracted using a procedure slightly modified, developed by the GenoSol platform for application in large-scale soil surveys (Terrat et al., 2012) organic waste product Compost issued from the co-composting of green wastes with sewage sludge pcr conditions 5ng of DNA were used for a 25 µl PCR conducted under the following conditions: initial denaturation:94degC_3.0min;35 cycles of denaturation:94degC_0.5min;annealing:52degC_1.0min;elongation:72degC_1.0min;final elongation:72degC_5.0min pcr primers FR1:5'-ANCCATTCAATCGGTANT-3';FF390:5'-CGATAACGAACGAGACCT-3' project name SOERE_PRO_2014 sample name B4T2_18S sample volume or weight for DNA extraction 1 sequencing method Illumina soil environmental package soil target gene 16S rRNA gene ERS1237088 SAMEA4065978 410658 soil metagenome Soil from Colmar, sampled one year after organic waste product apllication ENA first public 2017-06-01 ENA last update 2016-10-21 External Id SAMEA4065978 INSDC center alias INRA-CMSE INSDC center name UMR Agroecology, INRA - Burgundy University, Dijon, France INSDC first public 2017-06-01T17:02:03Z INSDC last update 2016-10-21T09:34:42Z INSDC status public Submitter Id B4T3_18S city Colmar collection date 2012-03 common name SOERE_PRO_2012 depth 0.2 environment (biome) cropland biome environment (feature) microbial feature environment (material) arable soil geographic location (country and/or sea) France geographic location (elevation) 175 geographic location (latitude) 48.0833 geographic location (longitude) 7.3667 geographic location (region and locality) Alsace history/previous land use 12 years of organic waste application (every 2 years) horizon A horizon investigation type metagenome multiplex identifiers CGTGTCTCTA nucleic acid amplification 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation: 94degC_3min; 35 cycles of: denaturation: 94degC_1min; annealing: 52degC_1min; elongation: 72degC_1min, final elongation: 72degC_5min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5 position to specifically identify each sample and avoid PCR biases. nucleic acid extraction DNA was extracted using a procedure slightly modified, developed by the GenoSol platform for application in large-scale soil surveys (Terrat et al., 2012) organic waste product Biowaste compost pcr conditions 5ng of DNA were used for a 25 µl PCR conducted under the following conditions: initial denaturation:94degC_3.0min;35 cycles of denaturation:94degC_0.5min;annealing:52degC_1.0min;elongation:72degC_1.0min;final elongation:72degC_5.0min pcr primers FR1:5'-ANCCATTCAATCGGTANT-3';FF390:5'-CGATAACGAACGAGACCT-3' project name SOERE_PRO_2014 sample name B4T3_18S sample volume or weight for DNA extraction 1 sequencing method Illumina soil environmental package soil target gene 16S rRNA gene ERS1237089 SAMEA4065979 410658 soil metagenome Soil from Colmar, sampled one year after organic waste product apllication ENA first public 2017-06-01 ENA last update 2016-10-21 External Id SAMEA4065979 INSDC center alias INRA-CMSE INSDC center name UMR Agroecology, INRA - Burgundy University, Dijon, France INSDC first public 2017-06-01T17:02:03Z INSDC last update 2016-10-21T09:34:42Z INSDC status public Submitter Id B4T4_18S city Colmar collection date 2012-03 common name SOERE_PRO_2012 depth 0.2 environment (biome) cropland biome environment (feature) microbial feature environment (material) arable soil geographic location (country and/or sea) France geographic location (elevation) 175 geographic location (latitude) 48.0833 geographic location (longitude) 7.3667 geographic location (region and locality) Alsace history/previous land use 12 years of organic waste application (every 2 years) horizon A horizon investigation type metagenome multiplex identifiers CATAGTAGTG nucleic acid amplification 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation: 94degC_3min; 35 cycles of: denaturation: 94degC_1min; annealing: 52degC_1min; elongation: 72degC_1min, final elongation: 72degC_5min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5 position to specifically identify each sample and avoid PCR biases. nucleic acid extraction DNA was extracted using a procedure slightly modified, developed by the GenoSol platform for application in large-scale soil surveys (Terrat et al., 2012) organic waste product Farmyard manure pcr conditions 5ng of DNA were used for a 25 µl PCR conducted under the following conditions: initial denaturation:94degC_3.0min;35 cycles of denaturation:94degC_0.5min;annealing:52degC_1.0min;elongation:72degC_1.0min;final elongation:72degC_5.0min pcr primers FR1:5'-ANCCATTCAATCGGTANT-3';FF390:5'-CGATAACGAACGAGACCT-3' project name SOERE_PRO_2014 sample name B4T4_18S sample volume or weight for DNA extraction 1 sequencing method Illumina soil environmental package soil target gene 16S rRNA gene ERS1237090 SAMEA4065980 410658 soil metagenome Soil from Colmar, sampled one year after organic waste product apllication ENA first public 2017-06-01 ENA last update 2016-10-21 External Id SAMEA4065980 INSDC center alias INRA-CMSE INSDC center name UMR Agroecology, INRA - Burgundy University, Dijon, France INSDC first public 2017-06-01T17:02:03Z INSDC last update 2016-10-21T09:34:42Z INSDC status public Submitter Id B4T1_18S city Colmar collection date 2012-03 common name SOERE_PRO_2012 depth 0.2 environment (biome) cropland biome environment (feature) microbial feature environment (material) arable soil geographic location (country and/or sea) France geographic location (elevation) 175 geographic location (latitude) 48.0833 geographic location (longitude) 7.3667 geographic location (region and locality) Alsace history/previous land use 12 years of organic waste application (every 2 years) horizon A horizon investigation type metagenome multiplex identifiers CGATCGTATA nucleic acid amplification 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation: 94degC_3min; 35 cycles of: denaturation: 94degC_1min; annealing: 52degC_1min; elongation: 72degC_1min, final elongation: 72degC_5min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5 position to specifically identify each sample and avoid PCR biases. nucleic acid extraction DNA was extracted using a procedure slightly modified, developed by the GenoSol platform for application in large-scale soil surveys (Terrat et al., 2012) organic waste product Non-composted sewage sludge pcr conditions 5ng of DNA were used for a 25 µl PCR conducted under the following conditions: initial denaturation:94degC_3.0min;35 cycles of denaturation:94degC_0.5min;annealing:52degC_1.0min;elongation:72degC_1.0min;final elongation:72degC_5.0min pcr primers FR1:5'-ANCCATTCAATCGGTANT-3';FF390:5'-CGATAACGAACGAGACCT-3' project name SOERE_PRO_2014 sample name B4T1_18S sample volume or weight for DNA extraction 1 sequencing method Illumina soil environmental package soil target gene 16S rRNA gene ERS1237091 SAMEA4065981 410658 soil metagenome Soil from Colmar, sampled one year after / No organic waste product apllication ENA first public 2017-06-01 ENA last update 2016-10-21 External Id SAMEA4065981 INSDC center alias INRA-CMSE INSDC center name UMR Agroecology, INRA - Burgundy University, Dijon, France INSDC first public 2017-06-01T17:02:03Z INSDC last update 2016-10-21T09:34:42Z INSDC status public Submitter Id B4T6_18S city Colmar collection date 2012-03 common name SOERE_PRO_2012 depth 0.2 environment (biome) cropland biome environment (feature) microbial feature environment (material) arable soil geographic location (country and/or sea) France geographic location (elevation) 175 geographic location (latitude) 48.0833 geographic location (longitude) 7.3667 geographic location (region and locality) Alsace history/previous land use 12 years of organic waste application (every 2 years) horizon A horizon investigation type metagenome multiplex identifiers TGTACTACTC nucleic acid amplification 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation: 94degC_3min; 35 cycles of: denaturation: 94degC_1min; annealing: 52degC_1min; elongation: 72degC_1min, final elongation: 72degC_5min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5 position to specifically identify each sample and avoid PCR biases. nucleic acid extraction DNA was extracted using a procedure slightly modified, developed by the GenoSol platform for application in large-scale soil surveys (Terrat et al., 2012) organic waste product Unamended soil pcr conditions 5ng of DNA were used for a 25 µl PCR conducted under the following conditions: initial denaturation:94degC_3.0min;35 cycles of denaturation:94degC_0.5min;annealing:52degC_1.0min;elongation:72degC_1.0min;final elongation:72degC_5.0min pcr primers FR1:5'-ANCCATTCAATCGGTANT-3';FF390:5'-CGATAACGAACGAGACCT-3' project name SOERE_PRO_2014 sample name B4T6_18S sample volume or weight for DNA extraction 1 sequencing method Illumina soil environmental package soil target gene 16S rRNA gene ERS1237092 SAMEA4065982 410658 soil metagenome Soil from Colmar, sampled one year after organic waste product apllication ENA first public 2017-06-01 ENA last update 2016-10-21 External Id SAMEA4065982 INSDC center alias INRA-CMSE INSDC center name UMR Agroecology, INRA - Burgundy University, Dijon, France INSDC first public 2017-06-01T17:02:03Z INSDC last update 2016-10-21T09:34:42Z INSDC status public Submitter Id B4T5_18S city Colmar collection date 2012-03 common name SOERE_PRO_2012 depth 0.2 environment (biome) cropland biome environment (feature) microbial feature environment (material) arable soil geographic location (country and/or sea) France geographic location (elevation) 175 geographic location (latitude) 48.0833 geographic location (longitude) 7.3667 geographic location (region and locality) Alsace history/previous land use 12 years of organic waste application (every 2 years) horizon A horizon investigation type metagenome multiplex identifiers CACGTGTCGC nucleic acid amplification 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation: 94degC_3min; 35 cycles of: denaturation: 94degC_1min; annealing: 52degC_1min; elongation: 72degC_1min, final elongation: 72degC_5min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5 position to specifically identify each sample and avoid PCR biases. nucleic acid extraction DNA was extracted using a procedure slightly modified, developed by the GenoSol platform for application in large-scale soil surveys (Terrat et al., 2012) organic waste product Farmyard manure compost pcr conditions 5ng of DNA were used for a 25 µl PCR conducted under the following conditions: initial denaturation:94degC_3.0min;35 cycles of denaturation:94degC_0.5min;annealing:52degC_1.0min;elongation:72degC_1.0min;final elongation:72degC_5.0min pcr primers FR1:5'-ANCCATTCAATCGGTANT-3';FF390:5'-CGATAACGAACGAGACCT-3' project name SOERE_PRO_2014 sample name B4T5_18S sample volume or weight for DNA extraction 1 sequencing method Illumina soil environmental package soil target gene 16S rRNA gene ERS1237093 SAMEA4065983 410658 soil metagenome Soil from Feucherolles, sampled one year after organic waste product apllication ENA first public 2017-06-01 ENA last update 2016-10-21 External Id SAMEA4065983 INSDC center alias INRA-CMSE INSDC center name UMR Agroecology, INRA - Burgundy University, Dijon, France INSDC first public 2017-06-01T17:02:03Z INSDC last update 2016-10-21T09:34:42Z INSDC status public Submitter Id 2012_407_16S city Feucherolles collection date 2012-03 common name SOERE_PRO_2012 depth 0.2 environment (biome) cropland biome environment (feature) microbial feature environment (material) arable soil geographic location (country and/or sea) France geographic location (elevation) 150 geographic location (latitude) 48.8667 geographic location (longitude) 1.9667 geographic location (region and locality) Ile de France history/previous land use 14 years of organic waste application (every 2 years) horizon A horizon investigation type metagenome multiplex identifiers CGTACAGTCA nucleic acid amplification 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation: 94degC_2 min; 35 cycles of denaturation: 94degC_30sec; annealing: 52degC_30sec; elongation: 72degC_1min; final elongation: 72degC_7min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5' position to specifically identify each sample and avoid PCR biases. nucleic acid extraction DNA was extracted using a procedure slightly modified, developed by the GenoSol platform for application in large-scale soil surveys (Terrat et al., 2012) organic waste product Municipal solid waste compost pcr conditions 5ng of DNA were used for a 25 µl PCR conducted under the following conditions: initial denaturation:94degC_2.0min;35 cycles of denaturation:94degC_0.5min;annealing:52degC_0.5min;elongation:72degC_1.0min;final elongation:72degC_7.0min pcr primers F479:5'-CAGCMGCYGCNGTAANAC-3'; R888:5'CCGYCAATTCMTTTRAGT-3' project name SOERE_PRO_2014 sample name 2012_407_16S sample volume or weight for DNA extraction 1 sequencing method Illumina soil environmental package soil target gene 16S rRNA gene