ERS1262049 SAMEA4350600 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350600 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_CW_Sample1 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_CW_Sample1 stimulus Alternating temperature strain AnTat 1.1E time 0 ERS1262050 SAMEA4350601 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350601 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_CW_Sample10 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_CW_Sample10 stimulus Alternating temperature strain AnTat 1.1E time 36 ERS1262051 SAMEA4350602 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350602 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_CW_Sample11 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_CW_Sample11 stimulus Alternating temperature strain AnTat 1.1E time 40 ERS1262052 SAMEA4350603 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350603 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_CW_Sample12 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_CW_Sample12 stimulus Alternating temperature strain AnTat 1.1E time 44 ERS1262053 SAMEA4350604 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350604 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_CW_Sample13 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_CW_Sample13 stimulus Alternating temperature strain AnTat 1.1E time 48 ERS1262054 SAMEA4350605 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350605 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_CW_Sample2 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_CW_Sample2 stimulus Alternating temperature strain AnTat 1.1E time 4 ERS1262055 SAMEA4350606 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350606 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_CW_Sample3 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_CW_Sample3 stimulus Alternating temperature strain AnTat 1.1E time 8 ERS1262056 SAMEA4350607 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350607 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_CW_Sample4 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_CW_Sample4 stimulus Alternating temperature strain AnTat 1.1E time 12 ERS1262057 SAMEA4350608 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350608 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_CW_Sample5 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_CW_Sample5 stimulus Alternating temperature strain AnTat 1.1E time 16 ERS1262058 SAMEA4350609 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350609 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_CW_Sample6 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_CW_Sample6 stimulus Alternating temperature strain AnTat 1.1E time 20 ERS1262059 SAMEA4350610 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350610 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_CW_Sample7 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_CW_Sample7 stimulus Alternating temperature strain AnTat 1.1E time 24 ERS1262060 SAMEA4350611 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350611 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_CW_Sample8 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_CW_Sample8 stimulus Alternating temperature strain AnTat 1.1E time 28 ERS1262061 SAMEA4350612 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350612 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_CW_Sample9 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_CW_Sample9 stimulus Alternating temperature strain AnTat 1.1E time 32 ERS1262062 SAMEA4350613 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350613 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_DD_Sample1 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_DD_Sample1 stimulus Constant darkness strain AnTat 1.1E time 0 ERS1262063 SAMEA4350614 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350614 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_DD_Sample10 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_DD_Sample10 stimulus Constant darkness strain AnTat 1.1E time 36 ERS1262064 SAMEA4350615 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350615 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_DD_Sample11 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_DD_Sample11 stimulus Constant darkness strain AnTat 1.1E time 40 ERS1262065 SAMEA4350616 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350616 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_DD_Sample12 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_DD_Sample12 stimulus Constant darkness strain AnTat 1.1E time 44 ERS1262066 SAMEA4350617 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350617 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_DD_Sample13 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_DD_Sample13 stimulus Constant darkness strain AnTat 1.1E time 48 ERS1262067 SAMEA4350618 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350618 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_DD_Sample2 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_DD_Sample2 stimulus Constant darkness strain AnTat 1.1E time 4 ERS1262068 SAMEA4350619 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350619 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_DD_Sample3 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_DD_Sample3 stimulus Constant darkness strain AnTat 1.1E time 8 ERS1262069 SAMEA4350620 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350620 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_DD_Sample4 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_DD_Sample4 stimulus Constant darkness strain AnTat 1.1E time 12 ERS1262070 SAMEA4350621 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350621 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_DD_Sample5 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_DD_Sample5 stimulus Constant darkness strain AnTat 1.1E time 16 ERS1262071 SAMEA4350622 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350622 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_DD_Sample6 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_DD_Sample6 stimulus Constant darkness strain AnTat 1.1E time 20 ERS1262072 SAMEA4350623 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350623 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_DD_Sample7 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_DD_Sample7 stimulus Constant darkness strain AnTat 1.1E time 24 ERS1262073 SAMEA4350624 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350624 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_DD_Sample8 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_DD_Sample8 stimulus Constant darkness strain AnTat 1.1E time 28 ERS1262074 SAMEA4350625 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350625 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:04Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_DD_Sample9 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_DD_Sample9 stimulus Constant darkness strain AnTat 1.1E time 32 ERS1262075 SAMEA4350626 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350626 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_LD_Sample1 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_LD_Sample1 stimulus Alternating light strain AnTat 1.1E time 0 ERS1262076 SAMEA4350627 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350627 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_LD_Sample10 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_LD_Sample10 stimulus Alternating light strain AnTat 1.1E time 36 ERS1262077 SAMEA4350628 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350628 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_LD_Sample11 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_LD_Sample11 stimulus Alternating light strain AnTat 1.1E time 40 ERS1262078 SAMEA4350629 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350629 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_LD_Sample12 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_LD_Sample12 stimulus Alternating light strain AnTat 1.1E time 44 ERS1262079 SAMEA4350630 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350630 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_LD_Sample13 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_LD_Sample13 stimulus Alternating light strain AnTat 1.1E time 48 ERS1262080 SAMEA4350631 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350631 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_LD_Sample2 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_LD_Sample2 stimulus Alternating light strain AnTat 1.1E time 4 ERS1262081 SAMEA4350632 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350632 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_LD_Sample3 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_LD_Sample3 stimulus Alternating light strain AnTat 1.1E time 8 ERS1262082 SAMEA4350633 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350633 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_LD_Sample4 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_LD_Sample4 stimulus Alternating light strain AnTat 1.1E time 12 ERS1262083 SAMEA4350634 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350634 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_LD_Sample5 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_LD_Sample5 stimulus Alternating light strain AnTat 1.1E time 16 ERS1262084 SAMEA4350635 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350635 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_LD_Sample6 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_LD_Sample6 stimulus Alternating light strain AnTat 1.1E time 20 ERS1262085 SAMEA4350636 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350636 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_LD_Sample7 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_LD_Sample7 stimulus Alternating light strain AnTat 1.1E time 24 ERS1262086 SAMEA4350637 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350637 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_LD_Sample8 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_LD_Sample8 stimulus Alternating light strain AnTat 1.1E time 28 ERS1262087 SAMEA4350638 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr in darkness and 12 hr under a LED 3W lamp. On the 4th and 5th days, parasites were kept in either these alternating conditions or in constant darkness. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. For the alternating light experiment, a LED 3W lamp was used to illuminate the cultures inside the incubator, alternating between light and darkness every 12 hr. Temperature was kept at 37°C and fluctuations were monitored and shown to be less than 0.1°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350638 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_LD_Sample9 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_LD_Sample9 stimulus Alternating light strain AnTat 1.1E time 32 ERS1262088 SAMEA4350639 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350639 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_WW_Sample1 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_WW_Sample1 stimulus Constant temperature strain AnTat 1.1E time 0 ERS1262089 SAMEA4350640 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350640 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_WW_Sample10 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_WW_Sample10 stimulus Constant temperature strain AnTat 1.1E time 36 ERS1262090 SAMEA4350641 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350641 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_WW_Sample11 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_WW_Sample11 stimulus Constant temperature strain AnTat 1.1E time 40 ERS1262091 SAMEA4350642 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350642 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_WW_Sample12 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_WW_Sample12 stimulus Constant temperature strain AnTat 1.1E time 44 ERS1262092 SAMEA4350643 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350643 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_WW_Sample13 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_WW_Sample13 stimulus Constant temperature strain AnTat 1.1E time 48 ERS1262093 SAMEA4350644 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350644 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_WW_Sample2 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_WW_Sample2 stimulus Constant temperature strain AnTat 1.1E time 4 ERS1262094 SAMEA4350645 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350645 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_WW_Sample3 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_WW_Sample3 stimulus Constant temperature strain AnTat 1.1E time 8 ERS1262095 SAMEA4350646 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350646 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_WW_Sample4 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_WW_Sample4 stimulus Constant temperature strain AnTat 1.1E time 12 ERS1262096 SAMEA4350647 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350647 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_WW_Sample5 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_WW_Sample5 stimulus Constant temperature strain AnTat 1.1E time 16 ERS1262097 SAMEA4350648 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350648 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_WW_Sample6 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_WW_Sample6 stimulus Constant temperature strain AnTat 1.1E time 20 ERS1262098 SAMEA4350649 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350649 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_WW_Sample7 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_WW_Sample7 stimulus Constant temperature strain AnTat 1.1E time 24 ERS1262099 SAMEA4350650 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350650 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_WW_Sample8 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_WW_Sample8 stimulus Constant temperature strain AnTat 1.1E time 28 ERS1262100 SAMEA4350651 5691 Trypanosoma brucei Protocols: Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 32°C and 12 hr at 37°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 37°C. Bloodstream forms were grown routinely in HMI-11 at 37°C in 5% CO2. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout two days. Temperature was varied every 12 hr from 32°C to 37°C or remained at constant 37°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350651 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_BSF_WW_Sample9 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_BSF_WW_Sample9 stimulus Constant temperature strain AnTat 1.1E time 32 ERS1262101 SAMEA4350652 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350652 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_CW_Sample1 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_CW_Sample1 stimulus Alternating temperature strain AnTat 1.1E time 0 ERS1262102 SAMEA4350653 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350653 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_CW_Sample10 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_CW_Sample10 stimulus Alternating temperature strain AnTat 1.1E time 36 ERS1262103 SAMEA4350654 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350654 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_CW_Sample11 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_CW_Sample11 stimulus Alternating temperature strain AnTat 1.1E time 40 ERS1262104 SAMEA4350655 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350655 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_CW_Sample12 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_CW_Sample12 stimulus Alternating temperature strain AnTat 1.1E time 44 ERS1262105 SAMEA4350656 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350656 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_CW_Sample13 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_CW_Sample13 stimulus Alternating temperature strain AnTat 1.1E time 48 ERS1262106 SAMEA4350657 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350657 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_CW_Sample2 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_CW_Sample2 stimulus Alternating temperature strain AnTat 1.1E time 4 ERS1262107 SAMEA4350658 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350658 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_CW_Sample3 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_CW_Sample3 stimulus Alternating temperature strain AnTat 1.1E time 8 ERS1262108 SAMEA4350659 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350659 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_CW_Sample4 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_CW_Sample4 stimulus Alternating temperature strain AnTat 1.1E time 12 ERS1262109 SAMEA4350660 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350660 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_CW_Sample5 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_CW_Sample5 stimulus Alternating temperature strain AnTat 1.1E time 16 ERS1262110 SAMEA4350661 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350661 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_CW_Sample6 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_CW_Sample6 stimulus Alternating temperature strain AnTat 1.1E time 20 ERS1262111 SAMEA4350662 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350662 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:05Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_CW_Sample7 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_CW_Sample7 stimulus Alternating temperature strain AnTat 1.1E time 24 ERS1262112 SAMEA4350663 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350663 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_CW_Sample8 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_CW_Sample8 stimulus Alternating temperature strain AnTat 1.1E time 28 ERS1262113 SAMEA4350664 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350664 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_CW_Sample9 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_CW_Sample9 stimulus Alternating temperature strain AnTat 1.1E time 32 ERS1262114 SAMEA4350665 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350665 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_WW_Sample1 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_WW_Sample1 stimulus Constant temperature strain AnTat 1.1E time 0 ERS1262115 SAMEA4350666 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350666 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_WW_Sample10 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_WW_Sample10 stimulus Constant temperature strain AnTat 1.1E time 36 ERS1262116 SAMEA4350667 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350667 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_WW_Sample11 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_WW_Sample11 stimulus Constant temperature strain AnTat 1.1E time 40 ERS1262117 SAMEA4350668 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350668 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_WW_Sample12 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_WW_Sample12 stimulus Constant temperature strain AnTat 1.1E time 44 ERS1262118 SAMEA4350669 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350669 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_WW_Sample13 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_WW_Sample13 stimulus Constant temperature strain AnTat 1.1E time 48 ERS1262119 SAMEA4350670 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350670 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_WW_Sample2 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_WW_Sample2 stimulus Constant temperature strain AnTat 1.1E time 4 ERS1262120 SAMEA4350671 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350671 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_WW_Sample3 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_WW_Sample3 stimulus Constant temperature strain AnTat 1.1E time 8 ERS1262121 SAMEA4350672 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350672 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_WW_Sample4 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_WW_Sample4 stimulus Constant temperature strain AnTat 1.1E time 12 ERS1262122 SAMEA4350673 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350673 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_WW_Sample5 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_WW_Sample5 stimulus Constant temperature strain AnTat 1.1E time 16 ERS1262123 SAMEA4350674 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350674 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_WW_Sample6 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_WW_Sample6 stimulus Constant temperature strain AnTat 1.1E time 20 ERS1262124 SAMEA4350675 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350675 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_WW_Sample7 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_WW_Sample7 stimulus Constant temperature strain AnTat 1.1E time 24 ERS1262125 SAMEA4350676 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350676 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_WW_Sample8 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_WW_Sample8 stimulus Constant temperature strain AnTat 1.1E time 28 ERS1262126 SAMEA4350677 5691 Trypanosoma brucei Protocols: Differentiation of bloodstream forms to procyclic forms was induced by addition of 6 mM cis-acconitate and a temperature change from 37°C to 28°C. Parasites were entrained/synchronized in vitro for three days by incubating parasites for 12 hr at 22°C and 12 hr at 27°C. On the 4th and 5th days, parasites were kept in either these alternating conditions or at constant 27ºC. Individual cultures of parasites were prepared, adjusting the initial parasite density so that at each time point parasite cultures would be at 10^6 parasites/mL. Samples were collected every 4 hr throughout the day. Temperature varied every 12 h from 23°C to 28°C or remained at constant 28°C. RNA was isolated from 10^7 Trypanosoma brucei cells with TRIzol reagent according to the manufacturer's instructions (Life Technologies). 1 µg of total RNA was enriched for mRNA using Poly-A beads for RNA-seq according to the manufacturer's instructions (Invitrogen). The removal of ribosomal RNAs was confirmed on a Bioanalyzer Nano Chip (Agilent Technologies). Sequencing libraries were constructed using TruSeq RNA Sample preparation protocol (Illumina). ENA first public 2017-02-14 ENA last update 2016-07-20 External Id SAMEA4350677 INSDC center alias Instituto de Medicina Molecular INSDC center name Instituto de Medicina Molecular INSDC first public 2017-02-14T17:02:27Z INSDC last update 2016-07-20T10:14:06Z INSDC status public Submitter Id E-MTAB-4952:TF_PF_WW_Sample9 broker name ArrayExpress cell line AnTat 1.1E 90-13 developmental stage Bloodstream form sample name E-MTAB-4952:TF_PF_WW_Sample9 stimulus Constant temperature strain AnTat 1.1E time 32