ERX1663116 E-MTAB-5047:S_lividans_TK24_2_Biolector_S2_p ERP016915 RNA-seq system comparison of Streptomyces lividans TK24 cultivated under micro-scale biolector and reference bioreactor conditions ERS1302218 SAMEA4390769 S_lividans_TK24_2_Biolector_S2_p RNA-Seq TRANSCRIPTOMIC RANDOM S. lividans TK24 grown in MTP BioLector (BL) a 48 well FlowerPlates (m2p-labs GmbH, Baesweiler, Germany), covered by a gas-permeable sealing foil (m2p-labs GmbH, Baesweiler, Germany) in modified minimal medium NMMP (D'Huys et al. 2011) was applied, containing 2.07 g/L NaH2PO4 x 1 H2O, 2.6 g/L K2HPO4, 0.6 MgSO4 x 7 H2O, 3.0 g/L (NH4)2SO4, 10 g/L D-glucose and 5 g/L BactoTM Casamino acids as well as several trace elements (1 mg/L ZnSO4 x 7 H2O, 1 mg/L FeSO4 x 7 H2O, 1 mg/L MnCl2 x 4 H2O, 1 mg/L CaCl2), harvested in late growth phase. Temperature and humidity was controlled in the incubation chamber of the BL device at 30 °C and 89 % respectively. Briefly, S. lividans TK24 cells were harvested at late growth phase. Total RNA was isolated from two biological replicates using RNeasy Mini Kit along with a DNase Kit (both from Qiagen). Initially RNA quality was checked by Trinean Xpose (Gentbrugge,Belgium) and Agilent RNA Nano 6000 kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). Samples contaminated with DNA were treated with DNase (Qiagen), cleaned as described above and rechecked by Xpose and Agilent Bioanalyzer. Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA, USA) was used to remove the ribosomal RNA molecules from the isolated total RNA. Removal of rRNA was checked by Agilent RNA Pico 6000 kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). 140 0 F Application Read Forward 1 1 R Application Read Reverse 71 Illumina HiSeq 1500 Experimental Factor: MTP BioLector (BL) cultivation growth condition ERX1663117 E-MTAB-5047:S_lividans_TK24_Bioreactor_R1_R2_100mM_MES_S2_p ERP016915 RNA-seq system comparison of Streptomyces lividans TK24 cultivated under micro-scale biolector and reference bioreactor conditions ERS1302219 SAMEA4390770 S_lividans_TK24_Bioreactor_R1_R2_100mM_MES_S2_p RNA-Seq TRANSCRIPTOMIC RANDOM S. lividans TK24 grown in a 1.5 L lab-scale system (DasGip, Jülich, Germany), equipped with two six blade, 46 mm Rushton turbines as well as sensors for DO (Hamilton, Visiferm DO 225) and pH (Mettler–Toledo, 405-DPAS-SC-K8S/225/120). in modified minimal medium NMMP (D'Huys et al. 2011) was applied, containing 2.07 g/L NaH2PO4 x 1 H2O, 2.6 g/L K2HPO4, 0.6 MgSO4 x 7 H2O, 3.0 g/L (NH4)2SO4, 10 g/L D-glucose and 5 g/L BactoTM Casamino acids as well as several trace elements (1 mg/L ZnSO4 x 7 H2O, 1 mg/L FeSO4 x 7 H2O, 1 mg/L MnCl2 x 4 H2O, 1 mg/L CaCl2), harvested in late growth phase. Temperature was controlled at 30 °C. Both, aeration and agitation were set to fixed values at 0.5 vvm and 800 rpm respectively. Briefly, S. lividans TK24 cells were harvested at late growth phase. Total RNA was isolated from two biological replicates using RNeasy Mini Kit along with a DNase Kit (both from Qiagen). Initially RNA quality was checked by Trinean Xpose (Gentbrugge,Belgium) and Agilent RNA Nano 6000 kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). Samples contaminated with DNA were treated with DNase (Qiagen), cleaned as described above and rechecked by Xpose and Agilent Bioanalyzer. Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA, USA) was used to remove the ribosomal RNA molecules from the isolated total RNA. Removal of rRNA was checked by Agilent RNA Pico 6000 kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). 140 0 F Application Read Forward 1 1 R Application Read Reverse 71 Illumina HiSeq 1500 Experimental Factor: Bioreactor 1.5 L lab-scale system (DasGip) growth condition