ERX1664609 qiita_ptid_55:1721.B11B qiita_ptid_55:1721.B11B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304258 SAMEA4392809 1721.B11B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTGTATCGCCAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BC27 number 88.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664610 qiita_ptid_55:1721.B11M qiita_ptid_55:1721.B11M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304259 SAMEA4392810 1721.B11M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGCCTCATGATG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BC26 number 87.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664611 qiita_ptid_55:1721.B11T qiita_ptid_55:1721.B11T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304260 SAMEA4392811 1721.B11T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTTATGACGGAT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BC25 number 86.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664612 qiita_ptid_55:1721.B12B qiita_ptid_55:1721.B12B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304261 SAMEA4392812 1721.B12B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGAATCGAAGCT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BC30 number 97.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664613 qiita_ptid_55:1721.B12M qiita_ptid_55:1721.B12M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304262 SAMEA4392813 1721.B12M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACGTGAGGAACG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BC29 number 96.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664614 qiita_ptid_55:1721.B12T qiita_ptid_55:1721.B12T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304263 SAMEA4392814 1721.B12T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CCAAACTCGTCG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BC28 number 95.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664615 qiita_ptid_55:1721.B15B qiita_ptid_55:1721.B15B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304264 SAMEA4392815 1721.B15B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATCGTGTGTTGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BC33 number 118.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664616 qiita_ptid_55:1721.B15M qiita_ptid_55:1721.B15M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304265 SAMEA4392816 1721.B15M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TATAGGCTCCGC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BC32 number 117.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664617 qiita_ptid_55:1721.B15T qiita_ptid_55:1721.B15T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304266 SAMEA4392817 1721.B15T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTGCAGTAAGTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BC31 number 116.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664618 qiita_ptid_55:1721.B16B qiita_ptid_55:1721.B16B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304267 SAMEA4392818 1721.B16B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCTGGGCATTGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BC36 number 127.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664619 qiita_ptid_55:1721.B16M qiita_ptid_55:1721.B16M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304268 SAMEA4392819 1721.B16M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCACTATACGCA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BC35 number 126.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664620 qiita_ptid_55:1721.B16T qiita_ptid_55:1721.B16T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304269 SAMEA4392820 1721.B16T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTTCCGCAGACA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BC34 number 125.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664621 qiita_ptid_55:1721.B17B qiita_ptid_55:1721.B17B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304270 SAMEA4392821 1721.B17B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTTGCATACCGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BD39 number 136.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664622 qiita_ptid_55:1721.B17M qiita_ptid_55:1721.B17M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304271 SAMEA4392822 1721.B17M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTAAACCGCGCC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BD38 number 135.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664623 qiita_ptid_55:1721.B17T qiita_ptid_55:1721.B17T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304272 SAMEA4392823 1721.B17T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CCAATGATAAGC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BD37 number 134.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664624 qiita_ptid_55:1721.B18B qiita_ptid_55:1721.B18B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304273 SAMEA4392824 1721.B18B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCAGGACGTATC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BD42 number 145.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664625 qiita_ptid_55:1721.B18M qiita_ptid_55:1721.B18M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304274 SAMEA4392825 1721.B18M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GGTCTAGGTCTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BD41 number 144.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664626 qiita_ptid_55:1721.B18T qiita_ptid_55:1721.B18T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304275 SAMEA4392826 1721.B18T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTGCACGATAAT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BD40 number 143.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664627 qiita_ptid_55:1721.B19B qiita_ptid_55:1721.B19B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304276 SAMEA4392827 1721.B19B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTCGCTTGCACA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BD45 number 154.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664628 qiita_ptid_55:1721.B19M qiita_ptid_55:1721.B19M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304277 SAMEA4392828 1721.B19M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GAATATACCTGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BD44 number 153.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664629 qiita_ptid_55:1721.B19T qiita_ptid_55:1721.B19T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304278 SAMEA4392829 1721.B19T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GAAAGGTGAGAA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BD43 number 152.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664630 qiita_ptid_55:1721.B20B qiita_ptid_55:1721.B20B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304279 SAMEA4392830 1721.B20B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TAGTGCATTCGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BD48 number 163.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664631 qiita_ptid_55:1721.B20M qiita_ptid_55:1721.B20M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304280 SAMEA4392831 1721.B20M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AATATCGGGATC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BD47 number 162.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664632 qiita_ptid_55:1721.B20T qiita_ptid_55:1721.B20T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304281 SAMEA4392832 1721.B20T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCTACCACGAAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BD46 number 161.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664633 qiita_ptid_55:1721.B21B qiita_ptid_55:1721.B21B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304282 SAMEA4392833 1721.B21B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGGATTGCTGTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BE51 number 171.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664634 qiita_ptid_55:1721.B21M qiita_ptid_55:1721.B21M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304283 SAMEA4392834 1721.B21M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTATCGGAAGAT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BE50 number 170.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664635 qiita_ptid_55:1721.B21T qiita_ptid_55:1721.B21T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304284 SAMEA4392835 1721.B21T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCAATGACCGCA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BE49 number 169.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664636 qiita_ptid_55:1721.B23B qiita_ptid_55:1721.B23B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304285 SAMEA4392836 1721.B23B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTAGCAGTATGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BE54 number 185.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664637 qiita_ptid_55:1721.B23M qiita_ptid_55:1721.B23M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304286 SAMEA4392837 1721.B23M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATCGAATCGAGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BE53 number 184.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664638 qiita_ptid_55:1721.B23T qiita_ptid_55:1721.B23T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304287 SAMEA4392838 1721.B23T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GGTACTGTACCA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BE52 number 183.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664639 qiita_ptid_55:1721.B24B qiita_ptid_55:1721.B24B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304288 SAMEA4392839 1721.B24B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CCTTCTGTATAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BE57 number 194.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664640 qiita_ptid_55:1721.B24M qiita_ptid_55:1721.B24M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304289 SAMEA4392840 1721.B24M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTATGGAGCTAT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BE56 number 193.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664641 qiita_ptid_55:1721.B24T qiita_ptid_55:1721.B24T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304290 SAMEA4392841 1721.B24T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTTAATGGCAGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BE55 number 192.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664642 qiita_ptid_55:1721.B25B qiita_ptid_55:1721.B25B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304291 SAMEA4392842 1721.B25B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCGAACCTATAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BE60 number 203.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664643 qiita_ptid_55:1721.B25M qiita_ptid_55:1721.B25M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304292 SAMEA4392843 1721.B25M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTCGTTTCAGTT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BE59 number 202.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664644 qiita_ptid_55:1721.B25T qiita_ptid_55:1721.B25T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304293 SAMEA4392844 1721.B25T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACGCTGTCGGTT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BE58 number 201.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664645 qiita_ptid_55:1721.B26B qiita_ptid_55:1721.B26B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304294 SAMEA4392845 1721.B26B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCGTTTCTTCAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BF63 number 212.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664646 qiita_ptid_55:1721.B26M qiita_ptid_55:1721.B26M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304295 SAMEA4392846 1721.B26M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CCAGTATCGCGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BF62 number 211.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664647 qiita_ptid_55:1721.B26T qiita_ptid_55:1721.B26T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304296 SAMEA4392847 1721.B26T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTCTCATATGCT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BF61 number 210.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664648 qiita_ptid_55:1721.B27B qiita_ptid_55:1721.B27B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304297 SAMEA4392848 1721.B27B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CCACTTGAGAGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BF66 number 221.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664649 qiita_ptid_55:1721.B27M qiita_ptid_55:1721.B27M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304298 SAMEA4392849 1721.B27M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GGATGCAGGATG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BF65 number 220.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664650 qiita_ptid_55:1721.B27T qiita_ptid_55:1721.B27T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304299 SAMEA4392850 1721.B27T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGTACCTAAGTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BF64 number 219.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664651 qiita_ptid_55:1721.B29B qiita_ptid_55:1721.B29B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304300 SAMEA4392851 1721.B29B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTCAAGTCAAAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BF69 number 236.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664652 qiita_ptid_55:1721.B29M qiita_ptid_55:1721.B29M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304301 SAMEA4392852 1721.B29M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGAATCCACCAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BF68 number 235.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664653 qiita_ptid_55:1721.B29T qiita_ptid_55:1721.B29T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304302 SAMEA4392853 1721.B29T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCACTTCATTTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BF67 number 234.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664654 qiita_ptid_55:1721.B2B qiita_ptid_55:1721.B2B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304303 SAMEA4392854 1721.B2B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACATGTCACGTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BA3 number 13.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664655 qiita_ptid_55:1721.B2M qiita_ptid_55:1721.B2M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304304 SAMEA4392855 1721.B2M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATGTTTAGACGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BA2 number 12.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664656 qiita_ptid_55:1721.B2T qiita_ptid_55:1721.B2T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304305 SAMEA4392856 1721.B2T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGTACCAGATCC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BA1 number 11.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664657 qiita_ptid_55:1721.B30B qiita_ptid_55:1721.B30B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304306 SAMEA4392857 1721.B30B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCAAGCAATACG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BF72 number 245.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664658 qiita_ptid_55:1721.B30M qiita_ptid_55:1721.B30M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304307 SAMEA4392858 1721.B30M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CACTGAGTACGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BF71 number 244.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664659 qiita_ptid_55:1721.B30T qiita_ptid_55:1721.B30T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304308 SAMEA4392859 1721.B30T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTACCTAGCCTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BF70 number 243.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664660 qiita_ptid_55:1721.B3B qiita_ptid_55:1721.B3B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304309 SAMEA4392860 1721.B3B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CAAGTCGAATAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BA6 number 21.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664661 qiita_ptid_55:1721.B3M qiita_ptid_55:1721.B3M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304310 SAMEA4392861 1721.B3M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTGGTCTTACGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BA5 number 20.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664662 qiita_ptid_55:1721.B3T qiita_ptid_55:1721.B3T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304311 SAMEA4392862 1721.B3T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTTTAGCGCTGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BA4 number 19.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664663 qiita_ptid_55:1721.B4B qiita_ptid_55:1721.B4B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304312 SAMEA4392863 1721.B4B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACCCTATTGCGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BA9 number 30.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664664 qiita_ptid_55:1721.B4M qiita_ptid_55:1721.B4M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304313 SAMEA4392864 1721.B4M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTCGGTCAACCA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BA8 number 29.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664665 qiita_ptid_55:1721.B4T qiita_ptid_55:1721.B4T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304314 SAMEA4392865 1721.B4T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCAAGTGTGAGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BA7 number 28.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664666 qiita_ptid_55:1721.B5B qiita_ptid_55:1721.B5B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304315 SAMEA4392866 1721.B5B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CATTTCGCACTT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BA12 number 39.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664667 qiita_ptid_55:1721.B5M qiita_ptid_55:1721.B5M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304316 SAMEA4392867 1721.B5M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACCACCGTAACC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BA11 number 38.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664668 qiita_ptid_55:1721.B5T qiita_ptid_55:1721.B5T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304317 SAMEA4392868 1721.B5T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCCGTTCGTTTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BA10 number 37.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664669 qiita_ptid_55:1721.B6B qiita_ptid_55:1721.B6B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304318 SAMEA4392869 1721.B6B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CAAACTGCGTTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BB15 number 47.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664670 qiita_ptid_55:1721.B6M qiita_ptid_55:1721.B6M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304319 SAMEA4392870 1721.B6M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGCGGGATTCAT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BB14 number 46.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664671 qiita_ptid_55:1721.B6T qiita_ptid_55:1721.B6T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304320 SAMEA4392871 1721.B6T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTAAGCGCCTGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BB13 number 45.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664672 qiita_ptid_55:1721.B7B qiita_ptid_55:1721.B7B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304321 SAMEA4392872 1721.B7B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GGCGAACTGAAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BB18 number 56.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664673 qiita_ptid_55:1721.B7M qiita_ptid_55:1721.B7M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304322 SAMEA4392873 1721.B7M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GACCGATAGGGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BB17 number 55.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664674 qiita_ptid_55:1721.B7T qiita_ptid_55:1721.B7T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304323 SAMEA4392874 1721.B7T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTAGACTCGGAA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BB16 number 54.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664675 qiita_ptid_55:1721.B8B qiita_ptid_55:1721.B8B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304324 SAMEA4392875 1721.B8B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATTCCCAGAACG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BB21 number 65.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664676 qiita_ptid_55:1721.B8M qiita_ptid_55:1721.B8M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304325 SAMEA4392876 1721.B8M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGGTGGTGGAGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BB20 number 64.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664677 qiita_ptid_55:1721.B8T qiita_ptid_55:1721.B8T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304326 SAMEA4392877 1721.B8T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGGCACTATCAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BB19 number 63.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664678 qiita_ptid_55:1721.B9B qiita_ptid_55:1721.B9B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304327 SAMEA4392878 1721.B9B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AAGCGTACATTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BB24 number 73.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664679 qiita_ptid_55:1721.B9M qiita_ptid_55:1721.B9M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304328 SAMEA4392879 1721.B9M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGAATAGCGCTT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BB23 number 72.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664680 qiita_ptid_55:1721.B9T qiita_ptid_55:1721.B9T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304329 SAMEA4392880 1721.B9T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGACGTTGCTAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location BB22 number 71.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Biochar primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664681 qiita_ptid_55:1721.BMC.7.1T.20.1 qiita_ptid_55:1721.BMC.7.1T.20.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304330 SAMEA4392881 1721.BMC.7.1T.20.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATAAGGTCGCCT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664682 qiita_ptid_55:1721.BMC.7.1T.20.2 qiita_ptid_55:1721.BMC.7.1T.20.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304331 SAMEA4392882 1721.BMC.7.1T.20.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTGCCCTTTGAT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664683 qiita_ptid_55:1721.BMC.7.1T.20.3 qiita_ptid_55:1721.BMC.7.1T.20.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304332 SAMEA4392883 1721.BMC.7.1T.20.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CCTGGAATTAAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664684 qiita_ptid_55:1721.BMC.7.1T.26.1 qiita_ptid_55:1721.BMC.7.1T.26.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304333 SAMEA4392884 1721.BMC.7.1T.26.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGAGACCCTACA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664685 qiita_ptid_55:1721.BMC.7.1T.26.2 qiita_ptid_55:1721.BMC.7.1T.26.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304334 SAMEA4392885 1721.BMC.7.1T.26.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AAGTATCCTGCG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664686 qiita_ptid_55:1721.BMC.7.1T.26.3 qiita_ptid_55:1721.BMC.7.1T.26.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304335 SAMEA4392886 1721.BMC.7.1T.26.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CAAATGGTCGTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664687 qiita_ptid_55:1721.BMC.7.1T.3.1 qiita_ptid_55:1721.BMC.7.1T.3.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304336 SAMEA4392887 1721.BMC.7.1T.3.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGAGATAGTTTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664688 qiita_ptid_55:1721.BMC.7.1T.3.2 qiita_ptid_55:1721.BMC.7.1T.3.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304337 SAMEA4392888 1721.BMC.7.1T.3.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGCGTCAAACTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664689 qiita_ptid_55:1721.BMC.7.1T.3.3 qiita_ptid_55:1721.BMC.7.1T.3.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304338 SAMEA4392889 1721.BMC.7.1T.3.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTGACACACGAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664690 qiita_ptid_55:1721.BMC.7.5T.18.1 qiita_ptid_55:1721.BMC.7.5T.18.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304339 SAMEA4392890 1721.BMC.7.5T.18.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATGCGAGACTTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664691 qiita_ptid_55:1721.BMC.7.5T.18.2 qiita_ptid_55:1721.BMC.7.5T.18.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304340 SAMEA4392891 1721.BMC.7.5T.18.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGCTTGTGTAGC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664692 qiita_ptid_55:1721.BMC.7.5T.18.3 qiita_ptid_55:1721.BMC.7.5T.18.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304341 SAMEA4392892 1721.BMC.7.5T.18.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATGAATGCGTCC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664693 qiita_ptid_55:1721.BMC.7.5T.21.1 qiita_ptid_55:1721.BMC.7.5T.21.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304342 SAMEA4392893 1721.BMC.7.5T.21.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GACTCTGCTCAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664694 qiita_ptid_55:1721.BMC.7.5T.21.2 qiita_ptid_55:1721.BMC.7.5T.21.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304343 SAMEA4392894 1721.BMC.7.5T.21.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CACGTACACGTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664695 qiita_ptid_55:1721.BMC.7.5T.21.3 qiita_ptid_55:1721.BMC.7.5T.21.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304344 SAMEA4392895 1721.BMC.7.5T.21.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CAGAGCTAATTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664696 qiita_ptid_55:1721.BMC.7.5T.5.1 qiita_ptid_55:1721.BMC.7.5T.5.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304345 SAMEA4392896 1721.BMC.7.5T.5.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CCACGGTACTTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664697 qiita_ptid_55:1721.BMC.7.5T.5.2 qiita_ptid_55:1721.BMC.7.5T.5.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304346 SAMEA4392897 1721.BMC.7.5T.5.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACTAGTTGGACC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664698 qiita_ptid_55:1721.BMC.7.5T.5.3 qiita_ptid_55:1721.BMC.7.5T.5.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304347 SAMEA4392898 1721.BMC.7.5T.5.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GATCAACCCACA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664699 qiita_ptid_55:1721.BMC.8.1T.11.1 qiita_ptid_55:1721.BMC.8.1T.11.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304348 SAMEA4392899 1721.BMC.8.1T.11.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTATCCAGTCCT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664700 qiita_ptid_55:1721.BMC.8.1T.11.2 qiita_ptid_55:1721.BMC.8.1T.11.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304349 SAMEA4392900 1721.BMC.8.1T.11.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTAAGACGTCGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664701 qiita_ptid_55:1721.BMC.8.1T.11.3 qiita_ptid_55:1721.BMC.8.1T.11.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304350 SAMEA4392901 1721.BMC.8.1T.11.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GGCTCAGATTCC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664702 qiita_ptid_55:1721.BMC.8.1T.30.1 qiita_ptid_55:1721.BMC.8.1T.30.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304351 SAMEA4392902 1721.BMC.8.1T.30.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTTGGTAGTGCC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664703 qiita_ptid_55:1721.BMC.8.1T.30.2 qiita_ptid_55:1721.BMC.8.1T.30.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304352 SAMEA4392903 1721.BMC.8.1T.30.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTGCTGCGCTTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664704 qiita_ptid_55:1721.BMC.8.1T.30.3 qiita_ptid_55:1721.BMC.8.1T.30.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304353 SAMEA4392904 1721.BMC.8.1T.30.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGTAGGAGGCAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664705 qiita_ptid_55:1721.BMC.8.5T.17.1 qiita_ptid_55:1721.BMC.8.5T.17.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304354 SAMEA4392905 1721.BMC.8.5T.17.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GGCACACCCTTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664706 qiita_ptid_55:1721.BMC.8.5T.17.2 qiita_ptid_55:1721.BMC.8.5T.17.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304355 SAMEA4392906 1721.BMC.8.5T.17.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTCCAGCTATGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664707 qiita_ptid_55:1721.BMC.8.5T.17.3 qiita_ptid_55:1721.BMC.8.5T.17.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304356 SAMEA4392907 1721.BMC.8.5T.17.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCGCGCAACTGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664708 qiita_ptid_55:1721.BMC.8.5T.2.1 qiita_ptid_55:1721.BMC.8.5T.2.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304357 SAMEA4392908 1721.BMC.8.5T.2.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACACATAAGTCG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664709 qiita_ptid_55:1721.BMC.8.5T.2.2 qiita_ptid_55:1721.BMC.8.5T.2.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304358 SAMEA4392909 1721.BMC.8.5T.2.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TACTGCCAGTGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664710 qiita_ptid_55:1721.BMC.8.5T.2.3 qiita_ptid_55:1721.BMC.8.5T.2.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304359 SAMEA4392910 1721.BMC.8.5T.2.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GAGTTTACGGTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664711 qiita_ptid_55:1721.BMC.8.5T.23.1 qiita_ptid_55:1721.BMC.8.5T.23.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304360 SAMEA4392911 1721.BMC.8.5T.23.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATTCCTCTCCAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664712 qiita_ptid_55:1721.BMC.8.5T.23.2 qiita_ptid_55:1721.BMC.8.5T.23.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304361 SAMEA4392912 1721.BMC.8.5T.23.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGGTTCATCCTT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664713 qiita_ptid_55:1721.BMC.8.5T.23.3 qiita_ptid_55:1721.BMC.8.5T.23.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304362 SAMEA4392913 1721.BMC.8.5T.23.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGCACTTTGAGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664714 qiita_ptid_55:1721.R10B qiita_ptid_55:1721.R10B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304363 SAMEA4392914 1721.R10B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGTCGTGCACAT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RC27 number 82.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664715 qiita_ptid_55:1721.R10M qiita_ptid_55:1721.R10M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304364 SAMEA4392915 1721.R10M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGAGCAATCCTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RC26 number 81.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664716 qiita_ptid_55:1721.R10T qiita_ptid_55:1721.R10T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304365 SAMEA4392916 1721.R10T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCGATATATCGC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RC25 number 80.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664717 qiita_ptid_55:1721.R11B qiita_ptid_55:1721.R11B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304366 SAMEA4392917 1721.R11B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CAAATTCGGGAT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RC30 number 91.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664718 qiita_ptid_55:1721.R11M qiita_ptid_55:1721.R11M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304367 SAMEA4392918 1721.R11M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGAGGGAAAGTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RC29 number 90.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664719 qiita_ptid_55:1721.R11T qiita_ptid_55:1721.R11T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304368 SAMEA4392919 1721.R11T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTATCTGCGCGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RC28 number 89.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664720 qiita_ptid_55:1721.R12B qiita_ptid_55:1721.R12B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304369 SAMEA4392920 1721.R12B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCATATGCACTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RC33 number 100.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664721 qiita_ptid_55:1721.R12M qiita_ptid_55:1721.R12M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304370 SAMEA4392921 1721.R12M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGTTACGAGCTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RC32 number 99.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664722 qiita_ptid_55:1721.R12T qiita_ptid_55:1721.R12T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304371 SAMEA4392922 1721.R12T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGATTGACCAAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RC31 number 98.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664723 qiita_ptid_55:1721.R13B qiita_ptid_55:1721.R13B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304372 SAMEA4392923 1721.R13B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TACGAGCCCTAA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RC36 number 106.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664724 qiita_ptid_55:1721.R13M qiita_ptid_55:1721.R13M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304373 SAMEA4392924 1721.R13M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTGCGTTAGCAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RC35 number 105.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664725 qiita_ptid_55:1721.R13T qiita_ptid_55:1721.R13T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304374 SAMEA4392925 1721.R13T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CAACTCCCGTGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RC34 number 104.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664726 qiita_ptid_55:1721.R14B qiita_ptid_55:1721.R14B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304375 SAMEA4392926 1721.R14B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACCATAGCTCCG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RD39 number 112.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664727 qiita_ptid_55:1721.R14M qiita_ptid_55:1721.R14M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304376 SAMEA4392927 1721.R14M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGCAGTCCTCGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RD38 number 111.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664728 qiita_ptid_55:1721.R14T qiita_ptid_55:1721.R14T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304377 SAMEA4392928 1721.R14T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CACTACGCTAGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RD37 number 110.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664729 qiita_ptid_55:1721.R15B qiita_ptid_55:1721.R15B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304378 SAMEA4392929 1721.R15B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GAGCCATCTGTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RD42 number 121.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664730 qiita_ptid_55:1721.R15M qiita_ptid_55:1721.R15M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304379 SAMEA4392930 1721.R15M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GAACACTTTGGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RD41 number 120.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664731 qiita_ptid_55:1721.R15T qiita_ptid_55:1721.R15T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304380 SAMEA4392931 1721.R15T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCGACATCTCTT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RD40 number 119.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664732 qiita_ptid_55:1721.R16B qiita_ptid_55:1721.R16B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304381 SAMEA4392932 1721.R16B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TAATACGGATCG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RD45 number 130.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664733 qiita_ptid_55:1721.R16M qiita_ptid_55:1721.R16M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304382 SAMEA4392933 1721.R16M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AAGGCGCTCCTT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RD44 number 129.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664734 qiita_ptid_55:1721.R16T qiita_ptid_55:1721.R16T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304383 SAMEA4392934 1721.R16T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTGGGTACACGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RD43 number 128.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664735 qiita_ptid_55:1721.R17B qiita_ptid_55:1721.R17B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304384 SAMEA4392935 1721.R17B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CATTCGTGGCGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RD48 number 139.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664736 qiita_ptid_55:1721.R17M qiita_ptid_55:1721.R17M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304385 SAMEA4392936 1721.R17M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGTGAATTCGGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RD47 number 138.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664737 qiita_ptid_55:1721.R17T qiita_ptid_55:1721.R17T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304386 SAMEA4392937 1721.R17T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCGGAATTAGAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RD46 number 137.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664738 qiita_ptid_55:1721.R18B qiita_ptid_55:1721.R18B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304387 SAMEA4392938 1721.R18B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GATGTTCGCTAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RE51 number 148.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664739 qiita_ptid_55:1721.R18M qiita_ptid_55:1721.R18M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304388 SAMEA4392939 1721.R18M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GGCCAGTTCCTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RE50 number 147.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664740 qiita_ptid_55:1721.R18T qiita_ptid_55:1721.R18T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304389 SAMEA4392940 1721.R18T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TACTACGTGGCC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RE49 number 146.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664741 qiita_ptid_55:1721.R19B qiita_ptid_55:1721.R19B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304390 SAMEA4392941 1721.R19B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATGATGAGCCTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RE54 number 157.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664742 qiita_ptid_55:1721.R19M qiita_ptid_55:1721.R19M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304391 SAMEA4392942 1721.R19M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACTCACAGGAAT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RE53 number 156.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664743 qiita_ptid_55:1721.R19T qiita_ptid_55:1721.R19T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304392 SAMEA4392943 1721.R19T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTATCTCCTGTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RE52 number 155.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664744 qiita_ptid_55:1721.R1B qiita_ptid_55:1721.R1B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304393 SAMEA4392944 1721.R1B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCTGTACGGATT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RA3 number 7.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664745 qiita_ptid_55:1721.R1M qiita_ptid_55:1721.R1M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304394 SAMEA4392945 1721.R1M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACGAGACTGATT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RA2 number 6.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664746 qiita_ptid_55:1721.R1T qiita_ptid_55:1721.R1T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304395 SAMEA4392946 1721.R1T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCCCTTGTCTCC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RA1 number 5.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664747 qiita_ptid_55:1721.R20B qiita_ptid_55:1721.R20B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304396 SAMEA4392947 1721.R20B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGTCGCAAATAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RE56 number 165.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664748 qiita_ptid_55:1721.R20M qiita_ptid_55:1721.R20M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304397 SAMEA4392948 1721.R20M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTCGACAGAGGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RE55 number 164.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664749 qiita_ptid_55:1721.R21B qiita_ptid_55:1721.R21B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304398 SAMEA4392949 1721.R21B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGTTGAGGCATT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RE59 number 174.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664750 qiita_ptid_55:1721.R21M qiita_ptid_55:1721.R21M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304399 SAMEA4392950 1721.R21M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TATACCGCTGCG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RE58 number 173.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664751 qiita_ptid_55:1721.R21T qiita_ptid_55:1721.R21T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304400 SAMEA4392951 1721.R21T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CATCCCTCTACT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RE57 number 172.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664752 qiita_ptid_55:1721.R22B qiita_ptid_55:1721.R22B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304401 SAMEA4392952 1721.R22B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTGGAGTCTCAT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RF62 number 180.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664753 qiita_ptid_55:1721.R22M qiita_ptid_55:1721.R22M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304402 SAMEA4392953 1721.R22M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGGTCAATTGAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RF61 number 179.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664754 qiita_ptid_55:1721.R22T qiita_ptid_55:1721.R22T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304403 SAMEA4392954 1721.R22T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACAATAGACACC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RE60 number 178.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664755 qiita_ptid_55:1721.R23B qiita_ptid_55:1721.R23B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304404 SAMEA4392955 1721.R23B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCTCTACCACTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RF65 number 188.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664756 qiita_ptid_55:1721.R23M qiita_ptid_55:1721.R23M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304405 SAMEA4392956 1721.R23M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGGCTTACGTGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RF64 number 187.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664757 qiita_ptid_55:1721.R23T qiita_ptid_55:1721.R23T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304406 SAMEA4392957 1721.R23T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCTCGAAGATTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RF63 number 186.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664758 qiita_ptid_55:1721.R24B qiita_ptid_55:1721.R24B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304407 SAMEA4392958 1721.R24B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTGTTGTCGTGC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RF68 number 197.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664759 qiita_ptid_55:1721.R24M qiita_ptid_55:1721.R24M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304408 SAMEA4392959 1721.R24M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTCACCTAGGAA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RF67 number 196.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664760 qiita_ptid_55:1721.R24T qiita_ptid_55:1721.R24T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304409 SAMEA4392960 1721.R24T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACTTCCAACTTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RF66 number 195.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664761 qiita_ptid_55:1721.R25B qiita_ptid_55:1721.R25B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304410 SAMEA4392961 1721.R25B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GATTCCGGCTCA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RF71 number 206.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664762 qiita_ptid_55:1721.R25M qiita_ptid_55:1721.R25M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304411 SAMEA4392962 1721.R25M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TATCGACACAAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RF70 number 205.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664763 qiita_ptid_55:1721.R25T qiita_ptid_55:1721.R25T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304412 SAMEA4392963 1721.R25T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CCACAGATCGAT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RF69 number 204.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664764 qiita_ptid_55:1721.R26B qiita_ptid_55:1721.R26B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304413 SAMEA4392964 1721.R26B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATGGGTTCCGTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RG74 number 215.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664765 qiita_ptid_55:1721.R26M qiita_ptid_55:1721.R26M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304414 SAMEA4392965 1721.R26M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GGTGACTAGTTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RG73 number 214.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664766 qiita_ptid_55:1721.R26T qiita_ptid_55:1721.R26T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304415 SAMEA4392966 1721.R26T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGTAATTGCCGC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RF72 number 213.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664767 qiita_ptid_55:1721.R27B qiita_ptid_55:1721.R27B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304416 SAMEA4392967 1721.R27B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATTCTGCCGAAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RG77 number 224.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664768 qiita_ptid_55:1721.R27M qiita_ptid_55:1721.R27M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304417 SAMEA4392968 1721.R27M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AACTAGTTCAGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RG76 number 223.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664769 qiita_ptid_55:1721.R27T qiita_ptid_55:1721.R27T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304418 SAMEA4392969 1721.R27T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TAGGCATGCTTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RG75 number 222.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664770 qiita_ptid_55:1721.R28B qiita_ptid_55:1721.R28B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304419 SAMEA4392970 1721.R28B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTGGTGGTTTCC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RG80 number 230.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664771 qiita_ptid_55:1721.R28M qiita_ptid_55:1721.R28M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304420 SAMEA4392971 1721.R28M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTACGATATGAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RG79 number 229.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664772 qiita_ptid_55:1721.R28T qiita_ptid_55:1721.R28T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304421 SAMEA4392972 1721.R28T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGCATGTCCCGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RG78 number 228.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664773 qiita_ptid_55:1721.R29B qiita_ptid_55:1721.R29B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304422 SAMEA4392973 1721.R29B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATGGCTGTCAGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RG83 number 239.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664774 qiita_ptid_55:1721.R29M qiita_ptid_55:1721.R29M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304423 SAMEA4392974 1721.R29M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGCGCTGAATGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RG82 number 238.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664775 qiita_ptid_55:1721.R29T qiita_ptid_55:1721.R29T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304424 SAMEA4392975 1721.R29T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TAGTATGCGCAA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RG81 number 237.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664776 qiita_ptid_55:1721.R2B qiita_ptid_55:1721.R2B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304425 SAMEA4392976 1721.R2B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGGTCAACGATA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RA5 number 15.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664777 qiita_ptid_55:1721.R2M qiita_ptid_55:1721.R2M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304426 SAMEA4392977 1721.R2M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATCACCAGGTGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RA4 number 14.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664778 qiita_ptid_55:1721.R30B qiita_ptid_55:1721.R30B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304427 SAMEA4392978 1721.R30B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCGTTCTAGCTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RH86 number 248.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664779 qiita_ptid_55:1721.R30M qiita_ptid_55:1721.R30M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304428 SAMEA4392979 1721.R30M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGTAAGATGCCT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RH85 number 247.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664780 qiita_ptid_55:1721.R30T qiita_ptid_55:1721.R30T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304429 SAMEA4392980 1721.R30T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTTCTCTTCTCG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RG84 number 246.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664781 qiita_ptid_55:1721.R3B qiita_ptid_55:1721.R3B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304430 SAMEA4392981 1721.R3B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGCGGAGGTTAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RA8 number 24.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664782 qiita_ptid_55:1721.R3M qiita_ptid_55:1721.R3M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304431 SAMEA4392982 1721.R3M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTCGTGTAGCCT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RA7 number 23.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664783 qiita_ptid_55:1721.R3T qiita_ptid_55:1721.R3T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304432 SAMEA4392983 1721.R3T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATCGCACAGTAA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RA6 number 22.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664784 qiita_ptid_55:1721.R4B qiita_ptid_55:1721.R4B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304433 SAMEA4392984 1721.R4B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACCGGTATGTAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RA11 number 33.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664785 qiita_ptid_55:1721.R4M qiita_ptid_55:1721.R4M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304434 SAMEA4392985 1721.R4M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TACAGCGCATAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RA10 number 32.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664786 qiita_ptid_55:1721.R4T qiita_ptid_55:1721.R4T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304435 SAMEA4392986 1721.R4T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATCCTTTGGTTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RA9 number 31.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664787 qiita_ptid_55:1721.R5M qiita_ptid_55:1721.R5M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304436 SAMEA4392987 1721.R5M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGCATACACTGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RB13 number 41.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664788 qiita_ptid_55:1721.R5T qiita_ptid_55:1721.R5T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304437 SAMEA4392988 1721.R5T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AATTGTGTCGGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RA12 number 40.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664789 qiita_ptid_55:1721.R6B qiita_ptid_55:1721.R6B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304438 SAMEA4392989 1721.R6B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GAATACCAAGTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RB16 number 50.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664790 qiita_ptid_55:1721.R6M qiita_ptid_55:1721.R6M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304439 SAMEA4392990 1721.R6M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACCAGTGACTCA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RB15 number 49.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664791 qiita_ptid_55:1721.R6T qiita_ptid_55:1721.R6T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304440 SAMEA4392991 1721.R6T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGTCGAACGAGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RB14 number 48.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664792 qiita_ptid_55:1721.R7B qiita_ptid_55:1721.R7B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304441 SAMEA4392992 1721.R7B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CATTATGGCGTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RB19 number 59.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664793 qiita_ptid_55:1721.R7M qiita_ptid_55:1721.R7M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304442 SAMEA4392993 1721.R7M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TAACGTGTGTGC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RB18 number 58.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664794 qiita_ptid_55:1721.R7T qiita_ptid_55:1721.R7T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304443 SAMEA4392994 1721.R7T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTAGATCGTGTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RB17 number 57.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664795 qiita_ptid_55:1721.R8B qiita_ptid_55:1721.R8B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304444 SAMEA4392995 1721.R8B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GATCTGCGATCC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RB21 number 67.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664796 qiita_ptid_55:1721.R8M qiita_ptid_55:1721.R8M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304445 SAMEA4392996 1721.R8M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CCAATACGCCTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RB20 number 66.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664797 qiita_ptid_55:1721.R9B qiita_ptid_55:1721.R9B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304446 SAMEA4392997 1721.R9B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCAACACCATCC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RB24 number 76.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664798 qiita_ptid_55:1721.R9M qiita_ptid_55:1721.R9M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304447 SAMEA4392998 1721.R9M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CAAACAACAGCT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RB23 number 75.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664799 qiita_ptid_55:1721.R9T qiita_ptid_55:1721.R9T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304448 SAMEA4392999 1721.R9T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CAGCTCATCAGC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location RB22 number 74.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Root primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664800 qiita_ptid_55:1721.S10B qiita_ptid_55:1721.S10B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304449 SAMEA4393000 1721.S10B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGTCATCGAATG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SC30 number 79.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664801 qiita_ptid_55:1721.S10M qiita_ptid_55:1721.S10M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304450 SAMEA4393001 1721.S10M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGGGATCAAATT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SC29 number 78.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664802 qiita_ptid_55:1721.S10T qiita_ptid_55:1721.S10T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304451 SAMEA4393002 1721.S10T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATAGCTTCGTGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SC28 number 77.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664803 qiita_ptid_55:1721.S11B qiita_ptid_55:1721.S11B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304452 SAMEA4393003 1721.S11B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCAAATCAGCCT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SC33 number 85.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664804 qiita_ptid_55:1721.S11M qiita_ptid_55:1721.S11M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304453 SAMEA4393004 1721.S11M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGCACCGGTCTT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SC32 number 84.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664805 qiita_ptid_55:1721.S11T qiita_ptid_55:1721.S11T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304454 SAMEA4393005 1721.S11T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATCTTGGAGTCG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SC31 number 83.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664806 qiita_ptid_55:1721.S12B qiita_ptid_55:1721.S12B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304455 SAMEA4393006 1721.S12B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCTTCAACTACC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SC36 number 94.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664807 qiita_ptid_55:1721.S12M qiita_ptid_55:1721.S12M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304456 SAMEA4393007 1721.S12M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGCGGCCTATTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SC35 number 93.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664808 qiita_ptid_55:1721.S12T qiita_ptid_55:1721.S12T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304457 SAMEA4393008 1721.S12T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCAAGCTGTCTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SC34 number 92.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664809 qiita_ptid_55:1721.S13B qiita_ptid_55:1721.S13B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304458 SAMEA4393009 1721.S13B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGCCGAGTAATC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SD39 number 103.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664810 qiita_ptid_55:1721.S13M qiita_ptid_55:1721.S13M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304459 SAMEA4393010 1721.S13M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TAAGATGCAGTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SD38 number 102.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664811 qiita_ptid_55:1721.S13T qiita_ptid_55:1721.S13T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304460 SAMEA4393011 1721.S13T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGGAATTCGGCT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SD37 number 101.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664812 qiita_ptid_55:1721.S14B qiita_ptid_55:1721.S14B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304461 SAMEA4393012 1721.S14B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CATAGCTCGGTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SD42 number 109.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664813 qiita_ptid_55:1721.S14M qiita_ptid_55:1721.S14M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304462 SAMEA4393013 1721.S14M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTAACCACCACC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SD41 number 108.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664814 qiita_ptid_55:1721.S14T qiita_ptid_55:1721.S14T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304463 SAMEA4393014 1721.S14T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACCTTGACAAGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SD40 number 107.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664815 qiita_ptid_55:1721.S15B qiita_ptid_55:1721.S15B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304464 SAMEA4393015 1721.S15B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACTACCTCTTCA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SD45 number 115.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664816 qiita_ptid_55:1721.S15M qiita_ptid_55:1721.S15M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304465 SAMEA4393016 1721.S15M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TATGGAGCTAGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SD44 number 114.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664817 qiita_ptid_55:1721.S15T qiita_ptid_55:1721.S15T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304466 SAMEA4393017 1721.S15T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AACCATGCCAAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SD43 number 113.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664818 qiita_ptid_55:1721.S16B qiita_ptid_55:1721.S16B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304467 SAMEA4393018 1721.S16B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTGTATGACAGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SD48 number 124.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664819 qiita_ptid_55:1721.S16M qiita_ptid_55:1721.S16M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304468 SAMEA4393019 1721.S16M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GGCCCAATATAA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SD47 number 123.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664820 qiita_ptid_55:1721.S16T qiita_ptid_55:1721.S16T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304469 SAMEA4393020 1721.S16T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GATGATAACCCA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SD46 number 122.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664821 qiita_ptid_55:1721.S17B qiita_ptid_55:1721.S17B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304470 SAMEA4393021 1721.S17B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGGTCTGTCTGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SE51 number 133.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664822 qiita_ptid_55:1721.S17M qiita_ptid_55:1721.S17M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304471 SAMEA4393022 1721.S17M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTACCACGGTAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SE50 number 132.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664823 qiita_ptid_55:1721.S17T qiita_ptid_55:1721.S17T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304472 SAMEA4393023 1721.S17T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GGTAAGTTTGAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SE49 number 131.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664824 qiita_ptid_55:1721.S18B qiita_ptid_55:1721.S18B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304473 SAMEA4393024 1721.S18B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACGGATGTTATG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SE54 number 142.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664825 qiita_ptid_55:1721.S18M qiita_ptid_55:1721.S18M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304474 SAMEA4393025 1721.S18M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTCTAGAGTGCG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SE53 number 141.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664826 qiita_ptid_55:1721.S18T qiita_ptid_55:1721.S18T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304475 SAMEA4393026 1721.S18T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTACATGTCGCC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SE52 number 140.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664827 qiita_ptid_55:1721.S19B qiita_ptid_55:1721.S19B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304476 SAMEA4393027 1721.S19B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACATCTAGCAGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SE57 number 151.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664828 qiita_ptid_55:1721.S19M qiita_ptid_55:1721.S19M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304477 SAMEA4393028 1721.S19M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTAGGAACCGGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SE56 number 150.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664829 qiita_ptid_55:1721.S19T qiita_ptid_55:1721.S19T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304478 SAMEA4393029 1721.S19T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTGAGGCTACAA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SE55 number 149.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664830 qiita_ptid_55:1721.S1B qiita_ptid_55:1721.S1B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304479 SAMEA4393030 1721.S1B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGCTTCGACAGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SA3 number 4.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664831 qiita_ptid_55:1721.S1M qiita_ptid_55:1721.S1M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304480 SAMEA4393031 1721.S1M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTCGATGTAAGC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SA2 number 3.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664832 qiita_ptid_55:1721.S1T qiita_ptid_55:1721.S1T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304481 SAMEA4393032 1721.S1T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGGAGCCTTGTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SA1 number 2.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664833 qiita_ptid_55:1721.S20B qiita_ptid_55:1721.S20B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304482 SAMEA4393033 1721.S20B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGCAAGCTAAGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SE60 number 160.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664834 qiita_ptid_55:1721.S20M qiita_ptid_55:1721.S20M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304483 SAMEA4393034 1721.S20M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CATGTAAGGCTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SE59 number 159.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664835 qiita_ptid_55:1721.S20T qiita_ptid_55:1721.S20T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304484 SAMEA4393035 1721.S20T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CCGACATTGTAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SE58 number 158.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664836 qiita_ptid_55:1721.S21B qiita_ptid_55:1721.S21B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304485 SAMEA4393036 1721.S21B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TAGGCTCGTGCT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SF63 number 168.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664837 qiita_ptid_55:1721.S21M qiita_ptid_55:1721.S21M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304486 SAMEA4393037 1721.S21M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGACAACCGAAT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SF62 number 167.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664838 qiita_ptid_55:1721.S21T qiita_ptid_55:1721.S21T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304487 SAMEA4393038 1721.S21T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTGTGTGCCATA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SF61 number 166.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664839 qiita_ptid_55:1721.S22B qiita_ptid_55:1721.S22B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304488 SAMEA4393039 1721.S22B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CAATTCTGCTTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SF66 number 177.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664840 qiita_ptid_55:1721.S22M qiita_ptid_55:1721.S22M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304489 SAMEA4393040 1721.S22M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTGCCTGGGTCA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SF65 number 176.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664841 qiita_ptid_55:1721.S22T qiita_ptid_55:1721.S22T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304490 SAMEA4393041 1721.S22T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTCCTTAAGGCG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SF64 number 175.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664842 qiita_ptid_55:1721.S23B qiita_ptid_55:1721.S23B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304491 SAMEA4393042 1721.S23B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CAATGTAGACAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SF69 number 182.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664843 qiita_ptid_55:1721.S23M qiita_ptid_55:1721.S23M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304492 SAMEA4393043 1721.S23M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AATCAGAGCTTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SF68 number 181.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664844 qiita_ptid_55:1721.S24B qiita_ptid_55:1721.S24B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304493 SAMEA4393044 1721.S24B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TACCTGTGTCTT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SF72 number 191.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664845 qiita_ptid_55:1721.S24M qiita_ptid_55:1721.S24M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304494 SAMEA4393045 1721.S24M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCCTTACGATAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SF71 number 190.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664846 qiita_ptid_55:1721.S24T qiita_ptid_55:1721.S24T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304495 SAMEA4393046 1721.S24T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGGCGATACGTT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SF70 number 189.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664847 qiita_ptid_55:1721.S25B qiita_ptid_55:1721.S25B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304496 SAMEA4393047 1721.S25B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACACCTGCGATC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SG75 number 200.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664848 qiita_ptid_55:1721.S25M qiita_ptid_55:1721.S25M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304497 SAMEA4393048 1721.S25M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GAAGACAGCGAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SG74 number 199.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664849 qiita_ptid_55:1721.S25T qiita_ptid_55:1721.S25T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304498 SAMEA4393049 1721.S25T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AACGAGGCAACG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SG73 number 198.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664850 qiita_ptid_55:1721.S26B qiita_ptid_55:1721.S26B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304499 SAMEA4393050 1721.S26B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTGCGACAAAGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SG78 number 209.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664851 qiita_ptid_55:1721.S26M qiita_ptid_55:1721.S26M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304500 SAMEA4393051 1721.S26M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACTAGCGTTCAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SG77 number 208.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664852 qiita_ptid_55:1721.S26T qiita_ptid_55:1721.S26T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304501 SAMEA4393052 1721.S26T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GGCGTTGCATTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SG76 number 207.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664853 qiita_ptid_55:1721.S27B qiita_ptid_55:1721.S27B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304502 SAMEA4393053 1721.S27B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCTGGAACGGTT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SG81 number 218.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664854 qiita_ptid_55:1721.S27M qiita_ptid_55:1721.S27M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304503 SAMEA4393054 1721.S27M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TACCACAACGAA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SG80 number 217.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664855 qiita_ptid_55:1721.S27T qiita_ptid_55:1721.S27T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304504 SAMEA4393055 1721.S27T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGCGAGTATATG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SG79 number 216.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664856 qiita_ptid_55:1721.S28B qiita_ptid_55:1721.S28B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304505 SAMEA4393056 1721.S28B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTATCCAAGTGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SG84 number 227.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664857 qiita_ptid_55:1721.S28M qiita_ptid_55:1721.S28M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304506 SAMEA4393057 1721.S28M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTCCTGTTAACC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SG83 number 226.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664858 qiita_ptid_55:1721.S28T qiita_ptid_55:1721.S28T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304507 SAMEA4393058 1721.S28T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTACTACCTCGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SG82 number 225.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664859 qiita_ptid_55:1721.S29B qiita_ptid_55:1721.S29B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304508 SAMEA4393059 1721.S29B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGTGGTGATGTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SH87 number 233.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664860 qiita_ptid_55:1721.S29M qiita_ptid_55:1721.S29M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304509 SAMEA4393060 1721.S29M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTGTCCGGATTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SH86 number 232.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664861 qiita_ptid_55:1721.S29T qiita_ptid_55:1721.S29T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304510 SAMEA4393061 1721.S29T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CAGTCTAGTACG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SH85 number 231.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664862 qiita_ptid_55:1721.S2B qiita_ptid_55:1721.S2B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304511 SAMEA4393062 1721.S2B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCACCTGTTGAA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SA6 number 10.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664863 qiita_ptid_55:1721.S2M qiita_ptid_55:1721.S2M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304512 SAMEA4393063 1721.S2M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AGATGTCCGTCA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SA5 number 9.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664864 qiita_ptid_55:1721.S2T qiita_ptid_55:1721.S2T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304513 SAMEA4393064 1721.S2T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATACGCATCAAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SA4 number 8.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664865 qiita_ptid_55:1721.S30B qiita_ptid_55:1721.S30B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304514 SAMEA4393065 1721.S30B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTTGGCGTTACA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SH90 number 242.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664866 qiita_ptid_55:1721.S30M qiita_ptid_55:1721.S30M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304515 SAMEA4393066 1721.S30M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CCGAAGATTCTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SH89 number 241.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664867 qiita_ptid_55:1721.S30T qiita_ptid_55:1721.S30T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304516 SAMEA4393067 1721.S30T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTTTCGTTCAAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SH88 number 240.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664868 qiita_ptid_55:1721.S3B qiita_ptid_55:1721.S3B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304517 SAMEA4393068 1721.S3B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTGTAAAGGTTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SA9 number 18.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664869 qiita_ptid_55:1721.S3M qiita_ptid_55:1721.S3M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304518 SAMEA4393069 1721.S3M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GAGGTTCTTGAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SA8 number 17.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664870 qiita_ptid_55:1721.S3T qiita_ptid_55:1721.S3T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304519 SAMEA4393070 1721.S3T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CCTAGAGAAACT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SA7 number 16.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664871 qiita_ptid_55:1721.S4B qiita_ptid_55:1721.S4B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304520 SAMEA4393071 1721.S4B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CTCTAGAAGAGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SA12 number 27.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664872 qiita_ptid_55:1721.S4M qiita_ptid_55:1721.S4M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304521 SAMEA4393072 1721.S4M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TACGGCAGTTCA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SA11 number 26.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664873 qiita_ptid_55:1721.S4T qiita_ptid_55:1721.S4T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304522 SAMEA4393073 1721.S4T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGAGTCATTGAG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SA10 number 25.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664874 qiita_ptid_55:1721.S5B qiita_ptid_55:1721.S5B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304523 SAMEA4393074 1721.S5B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGCTCCGTAGAA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SB15 number 36.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664875 qiita_ptid_55:1721.S5M qiita_ptid_55:1721.S5M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304524 SAMEA4393075 1721.S5M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CATGCGGATCCT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SB14 number 35.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664876 qiita_ptid_55:1721.S5T qiita_ptid_55:1721.S5T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304525 SAMEA4393076 1721.S5T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGCACAGTCGCT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SB13 number 34.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664877 qiita_ptid_55:1721.S6B qiita_ptid_55:1721.S6B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304526 SAMEA4393077 1721.S6B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AAGCAGATTGTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SB18 number 44.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664878 qiita_ptid_55:1721.S6M qiita_ptid_55:1721.S6M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304527 SAMEA4393078 1721.S6M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CGAGTTCATCGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SB17 number 43.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664879 qiita_ptid_55:1721.S6T qiita_ptid_55:1721.S6T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304528 SAMEA4393079 1721.S6T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGATAGGTACAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SB16 number 42.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664880 qiita_ptid_55:1721.S7B qiita_ptid_55:1721.S7B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304529 SAMEA4393080 1721.S7B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TAGACCGACTCC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SB21 number 53.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664881 qiita_ptid_55:1721.S7M qiita_ptid_55:1721.S7M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304530 SAMEA4393081 1721.S7M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCAGCGCCGTTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SB20 number 52.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664882 qiita_ptid_55:1721.S7T qiita_ptid_55:1721.S7T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304531 SAMEA4393082 1721.S7T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TAGAGGCGTAGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SB19 number 51.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664883 qiita_ptid_55:1721.S8B qiita_ptid_55:1721.S8B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304532 SAMEA4393083 1721.S8B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCTGTCGTCAAC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SB24 number 62.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664884 qiita_ptid_55:1721.S8M qiita_ptid_55:1721.S8M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304533 SAMEA4393084 1721.S8M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ACAGGAGGGTGT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SB23 number 61.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664885 qiita_ptid_55:1721.S8T qiita_ptid_55:1721.S8T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304534 SAMEA4393085 1721.S8T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTCAACGCTGTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SB22 number 60.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664886 qiita_ptid_55:1721.S9B qiita_ptid_55:1721.S9B ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304535 SAMEA4393086 1721.S9B OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TAAGCGTCTCGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SC27 number 70.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664887 qiita_ptid_55:1721.S9M qiita_ptid_55:1721.S9M ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304536 SAMEA4393087 1721.S9M OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AAGCTTGAAACC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SC26 number 69.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664888 qiita_ptid_55:1721.S9T qiita_ptid_55:1721.S9T ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304537 SAMEA4393088 1721.S9T OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode ATAGAGGCCATT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SC25 number 68.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664889 qiita_ptid_55:1721.SBB1 qiita_ptid_55:1721.SBB1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304538 SAMEA4393089 1721.SBB1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCGGAAACATGG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SH93 number 251.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664890 qiita_ptid_55:1721.SBB2 qiita_ptid_55:1721.SBB2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304539 SAMEA4393090 1721.SBB2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TCAATCGCTTTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SH96 number 254.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664891 qiita_ptid_55:1721.SBM1 qiita_ptid_55:1721.SBM1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304540 SAMEA4393091 1721.SBM1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTGCGGACCCTA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SH92 number 250.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664892 qiita_ptid_55:1721.SBM2 qiita_ptid_55:1721.SBM2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304541 SAMEA4393092 1721.SBM2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TGCATGACAGTC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SH95 number 253.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664893 qiita_ptid_55:1721.SBT1 qiita_ptid_55:1721.SBT1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304542 SAMEA4393093 1721.SBT1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GAAGTAGCGAGC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SH91 number 249.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664894 qiita_ptid_55:1721.SBT2 qiita_ptid_55:1721.SBT2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304543 SAMEA4393094 1721.SBT2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AACGTTAGTGTG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location SH94 number 252.0 pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 3/1/14 run_prefix Thomas_barley_soil_lane5_NoIndex_L005 samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664895 qiita_ptid_55:1721.Soil.NIL.100.N.10.1 qiita_ptid_55:1721.Soil.NIL.100.N.10.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304544 SAMEA4393095 1721.Soil.NIL.100.N.10.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTCTCTGAAAGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664896 qiita_ptid_55:1721.Soil.NIL.100.N.10.2 qiita_ptid_55:1721.Soil.NIL.100.N.10.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304545 SAMEA4393096 1721.Soil.NIL.100.N.10.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTTCTGCTTGTT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664897 qiita_ptid_55:1721.Soil.NIL.100.N.10.3 qiita_ptid_55:1721.Soil.NIL.100.N.10.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304546 SAMEA4393097 1721.Soil.NIL.100.N.10.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GTCAAGACCTCA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664898 qiita_ptid_55:1721.Soil.NIL.100.N.14.1 qiita_ptid_55:1721.Soil.NIL.100.N.14.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304547 SAMEA4393098 1721.Soil.NIL.100.N.14.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTGTTACGTTCC center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664899 qiita_ptid_55:1721.Soil.NIL.100.N.14.2 qiita_ptid_55:1721.Soil.NIL.100.N.14.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304548 SAMEA4393099 1721.Soil.NIL.100.N.14.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CAGTTCGAGATA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664900 qiita_ptid_55:1721.Soil.NIL.100.N.14.3 qiita_ptid_55:1721.Soil.NIL.100.N.14.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304549 SAMEA4393100 1721.Soil.NIL.100.N.14.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode AATGTCACCAGA center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664901 qiita_ptid_55:1721.Soil.NIL.100.N.22.1 qiita_ptid_55:1721.Soil.NIL.100.N.22.1 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304550 SAMEA4393101 1721.Soil.NIL.100.N.22.1 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode CAGCCTGCAAAT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664902 qiita_ptid_55:1721.Soil.NIL.100.N.22.2 qiita_ptid_55:1721.Soil.NIL.100.N.22.2 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304551 SAMEA4393102 1721.Soil.NIL.100.N.22.2 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode TTGCAAGTACCG center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4 ERX1664903 qiita_ptid_55:1721.Soil.NIL.100.N.22.3 qiita_ptid_55:1721.Soil.NIL.100.N.22.3 ERP016937 This project will obtain a comprehensive picture of the microbial community in bulk soil, roots and biochar particles in agricultural soils that were supplemented with various amounts of biochar as part of a field trial executed by the New South Wales Department of Primary Industries (DPI). A total of 253 samples will be analysed, that constitute a design that will allow us to understand where the most pronounced microbial changes occur (e.g. biochar particle versus soil) and what changes in the microbial communities occur over time. ERS1304552 SAMEA4393103 1721.Soil.NIL.100.N.22.3 OTHER METAGENOMIC PCR EMP V4 515f,806rbc protocol Illumina HiSeq 2000 barcode GCTTCTCTCACT center_name CCME center_project_name Thomas_barley_soil emp_status EMP illumina_technology HiSeq linker GT location Unknown number Unknown pcr_primers FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT plate Soil primer GTGTGCCAGCMGCCGCGGTAA run_center CCME run_date 1/30/12 run_prefix s_1_1_sequences samp_size 0.1, gram sample_center Thomas Lab, Sydney, Australia sequencing_meth sequencing by synthesis study_center CCME target_gene 16S rRNA target_subfragment V4