ERS8489582 SAMEA10838348 178606 Leptospirillum ferriphilum Protocols: Centrifugation of culture in late exponential phase at 8000 x g and 4째C for 15 minutes. Washing of cells with cold 10 mM H2SO4, and twice with 10 mM sodium citrate pH 7.0. Growth of L.ferriphulum in DSMZ 882 medium (pH1.8) supplemented with 20 g L-1 ferrous sulfate in a shaker at 37째C and 180 rpm. Isolation of total RNA by RNeasy Mini Kit from Qiagen. DNA removed by treatment with DNase I (New England Biolabs). Evaluation of purity and integrity of total RNA with Agilent Bioanalyzer 2100 and an RNA 600 Nano Kit (Agilent Technologies. For each sample 3 RNA preparations with RMNA integrity number above 7 from independent biological replicates pooled into one. Depletion of ribosomal RNA by MICROBExpress kit (Thermo Fisher). Preparation of cDNA library by TruSeq stranded mRNA library prep kit (Illumina). ENA first public 2022-03-31 ENA last update 2022-03-31 External Id SAMEA10838348 INSDC center alias Technische Universitat Bergakademie Freiberg Institute of Biosciences INSDC center name Technische Universitat Bergakademie Freiberg Institute of Biosciences INSDC first public 2022-03-31T00:17:45Z INSDC last update 2022-03-31T00:17:45Z INSDC status public Submitter Id E-MTAB-11136:Sample 1 broker name ArrayExpress organism part whole organism sample name E-MTAB-11136:Sample 1 strain DSM 14647 type-material type strain of Leptospirillum ferriphilum ERS8489583 SAMEA10838349 178606 Leptospirillum ferriphilum Protocols: Centrifugation of culture in late exponential phase at 8000 x g and 4째C for 15 minutes. Washing of cells with cold 10 mM H2SO4, and twice with 10 mM sodium citrate pH 7.0. Growth of L.ferriphulum in DSMZ 882 medium (pH1.8) supplemented with 20 g L-1 ferrous sulfate in a shaker at 37째C and 180 rpm. Isolation of total RNA by RNeasy Mini Kit from Qiagen. DNA removed by treatment with DNase I (New England Biolabs). Evaluation of purity and integrity of total RNA with Agilent Bioanalyzer 2100 and an RNA 600 Nano Kit (Agilent Technologies. For each sample 3 RNA preparations with RMNA integrity number above 7 from independent biological replicates pooled into one. Depletion of ribosomal RNA by MICROBExpress kit (Thermo Fisher). Preparation of cDNA library by TruSeq stranded mRNA library prep kit (Illumina). ENA first public 2022-03-31 ENA last update 2022-03-31 External Id SAMEA10838349 INSDC center alias Technische Universitat Bergakademie Freiberg Institute of Biosciences INSDC center name Technische Universitat Bergakademie Freiberg Institute of Biosciences INSDC first public 2022-03-31T00:17:45Z INSDC last update 2022-03-31T00:17:45Z INSDC status public Submitter Id E-MTAB-11136:Sample 2 broker name ArrayExpress organism part whole organism sample name E-MTAB-11136:Sample 2 strain DSM 14647 type-material type strain of Leptospirillum ferriphilum