ERX1787334 E-MTAB-4766_2:Sample 1_p E-MTAB-4766_2:Sample 1_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429925 SAMEA4530746 Sample 1_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 2 age Experimental Factor: 2 weeks 1x sugar diet ERX1787335 E-MTAB-4766_2:Sample 10_p E-MTAB-4766_2:Sample 10_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429926 SAMEA4530747 Sample 10_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 2 age Experimental Factor: 1 week 8x sugar, 1 week 1x sugar diet ERX1787336 E-MTAB-4766_2:Sample 11_p E-MTAB-4766_2:Sample 11_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429927 SAMEA4530748 Sample 11_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 2 age Experimental Factor: 1 week 8x sugar, 1 week 1x sugar diet ERX1787337 E-MTAB-4766_2:Sample 12_p E-MTAB-4766_2:Sample 12_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429928 SAMEA4530749 Sample 12_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 1 age Experimental Factor: 1 week 8x sugar diet ERX1787338 E-MTAB-4766_2:Sample 13_p E-MTAB-4766_2:Sample 13_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429929 SAMEA4530750 Sample 13_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 1 age Experimental Factor: 1 week 8x sugar diet ERX1787339 E-MTAB-4766_2:Sample 14_p E-MTAB-4766_2:Sample 14_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429930 SAMEA4530751 Sample 14_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 1 age Experimental Factor: 1 week 8x sugar diet ERX1787340 E-MTAB-4766_2:Sample 2_p E-MTAB-4766_2:Sample 2_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429931 SAMEA4530752 Sample 2_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 2 age Experimental Factor: 1 week 8x sugar, 1 week 1x sugar diet ERX1787341 E-MTAB-4766_2:Sample 3_p E-MTAB-4766_2:Sample 3_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429932 SAMEA4530753 Sample 3_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 2 age Experimental Factor: 2 weeks 1x sugar diet ERX1787342 E-MTAB-4766_2:Sample 4_p E-MTAB-4766_2:Sample 4_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429933 SAMEA4530754 Sample 4_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 2 age Experimental Factor: 2 weeks 1x sugar diet ERX1787343 E-MTAB-4766_2:Sample 5_p E-MTAB-4766_2:Sample 5_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429934 SAMEA4530755 Sample 5_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 2 age Experimental Factor: 2 weeks 1x sugar diet ERX1787344 E-MTAB-4766_2:Sample 6_p E-MTAB-4766_2:Sample 6_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429935 SAMEA4530756 Sample 6_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 1 age Experimental Factor: 1 week 1x sugar diet ERX1787345 E-MTAB-4766_2:Sample 7_p E-MTAB-4766_2:Sample 7_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429936 SAMEA4530757 Sample 7_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 1 age Experimental Factor: 1 week 1x sugar diet ERX1787346 E-MTAB-4766_2:Sample 8_p E-MTAB-4766_2:Sample 8_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429937 SAMEA4530758 Sample 8_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 1 age Experimental Factor: 1 week 1x sugar diet ERX1787347 E-MTAB-4766_2:Sample 9_p E-MTAB-4766_2:Sample 9_p ERP019485 Nutritional programming of lifespan: Excessive sugar consumption in early adulthood curtails Drosophila survival by inhibiting dFOXO ERS1429938 SAMEA4530759 Sample 9_p RNA-Seq TRANSCRIPTOMIC cDNA Flies were maintained on food containing 10% yeast, 5% sucrose, 1.5% agarose (all w/v; 1x sugar food) (Bass et al., 2007). Experimental flies developed at standardised densities on 1x sugar food and once-mated females we sorted on day two of adulthood onto the appropriate food. 8x sugar food contained 10% yeast, 40% sucrose, 1.5% agarose. RNA was extracted in Trizol by phenol-chloroform method. 64 0 F Application Read Forward 1 1 R Application Read Reverse 33 NextSeq 500 Experimental Factor: 2 age Experimental Factor: 1 week 8x sugar, 1 week 1x sugar diet