ERX1787534 E-MTAB-5190:GF1LPS_p ERP019492 RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice ERS1430331 SAMEA4531152 GF1LPS_p RNA-Seq TRANSCRIPTOMIC RANDOM Spenocytes were stained for CD3- CD19- CD11c+ MHC-II+ CD45+ and sorted to high purity by flow cytometry Isolated cells were plated in D10+ medium, stimulated with either PBS or LPS for 2h. The cells were collected. Total RNA was isolated by Trizol extraction Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany. The library preparation included a polyA enrichment. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: germ-free C57BL/6 strain Experimental Factor: wild type genotype genotype Experimental Factor: lipopolysaccharide stimulus ERX1787535 E-MTAB-5190:GF2LPS_p ERP019492 RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice ERS1430332 SAMEA4531153 GF2LPS_p RNA-Seq TRANSCRIPTOMIC RANDOM Spenocytes were stained for CD3- CD19- CD11c+ MHC-II+ CD45+ and sorted to high purity by flow cytometry Isolated cells were plated in D10+ medium, stimulated with either PBS or LPS for 2h. The cells were collected. Total RNA was isolated by Trizol extraction Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany. The library preparation included a polyA enrichment. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: germ-free C57BL/6 strain Experimental Factor: wild type genotype genotype Experimental Factor: lipopolysaccharide stimulus ERX1787536 E-MTAB-5190:GF1O_p ERP019492 RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice ERS1430333 SAMEA4531154 GF1O_p RNA-Seq TRANSCRIPTOMIC RANDOM Spenocytes were stained for CD3- CD19- CD11c+ MHC-II+ CD45+ and sorted to high purity by flow cytometry Isolated cells were plated in D10+ medium, stimulated with either PBS or LPS for 2h. The cells were collected. Total RNA was isolated by Trizol extraction Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany. The library preparation included a polyA enrichment. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: germ-free C57BL/6 strain Experimental Factor: wild type genotype genotype Experimental Factor: PBS stimulus ERX1787537 E-MTAB-5190:GF2O_p ERP019492 RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice ERS1430334 SAMEA4531155 GF2O_p RNA-Seq TRANSCRIPTOMIC RANDOM Spenocytes were stained for CD3- CD19- CD11c+ MHC-II+ CD45+ and sorted to high purity by flow cytometry Isolated cells were plated in D10+ medium, stimulated with either PBS or LPS for 2h. The cells were collected. Total RNA was isolated by Trizol extraction Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany. The library preparation included a polyA enrichment. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: germ-free C57BL/6 strain Experimental Factor: wild type genotype genotype Experimental Factor: PBS stimulus ERX1787538 E-MTAB-5190:IFNAR1LPS_p ERP019492 RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice ERS1430335 SAMEA4531156 IFNAR1LPS_p RNA-Seq TRANSCRIPTOMIC RANDOM Spenocytes were stained for CD3- CD19- CD11c+ MHC-II+ CD45+ and sorted to high purity by flow cytometry Isolated cells were plated in D10+ medium, stimulated with either PBS or LPS for 2h. The cells were collected. Total RNA was isolated by Trizol extraction Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany. The library preparation included a polyA enrichment. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: C57BL/6 strain Experimental Factor: Ifnar1 knockout genotype Experimental Factor: lipopolysaccharide stimulus ERX1787539 E-MTAB-5190:IFNAR2LPS_p ERP019492 RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice ERS1430336 SAMEA4531157 IFNAR2LPS_p RNA-Seq TRANSCRIPTOMIC RANDOM Spenocytes were stained for CD3- CD19- CD11c+ MHC-II+ CD45+ and sorted to high purity by flow cytometry Isolated cells were plated in D10+ medium, stimulated with either PBS or LPS for 2h. The cells were collected. Total RNA was isolated by Trizol extraction Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany. The library preparation included a polyA enrichment. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: C57BL/6 strain Experimental Factor: Ifnar1 knockout genotype Experimental Factor: lipopolysaccharide stimulus ERX1787540 E-MTAB-5190:IFNAR1O_p ERP019492 RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice ERS1430337 SAMEA4531158 IFNAR1O_p RNA-Seq TRANSCRIPTOMIC RANDOM Spenocytes were stained for CD3- CD19- CD11c+ MHC-II+ CD45+ and sorted to high purity by flow cytometry Isolated cells were plated in D10+ medium, stimulated with either PBS or LPS for 2h. The cells were collected. Total RNA was isolated by Trizol extraction Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany. The library preparation included a polyA enrichment. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: C57BL/6 strain Experimental Factor: Ifnar1 knockout genotype Experimental Factor: PBS stimulus ERX1787541 E-MTAB-5190:IFNAR2O_p ERP019492 RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice ERS1430338 SAMEA4531159 IFNAR2O_p RNA-Seq TRANSCRIPTOMIC RANDOM Spenocytes were stained for CD3- CD19- CD11c+ MHC-II+ CD45+ and sorted to high purity by flow cytometry Isolated cells were plated in D10+ medium, stimulated with either PBS or LPS for 2h. The cells were collected. Total RNA was isolated by Trizol extraction Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany. The library preparation included a polyA enrichment. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: C57BL/6 strain Experimental Factor: Ifnar1 knockout genotype Experimental Factor: PBS stimulus ERX1787542 E-MTAB-5190:WT1LPS_p ERP019492 RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice ERS1430339 SAMEA4531160 WT1LPS_p RNA-Seq TRANSCRIPTOMIC RANDOM Spenocytes were stained for CD3- CD19- CD11c+ MHC-II+ CD45+ and sorted to high purity by flow cytometry Isolated cells were plated in D10+ medium, stimulated with either PBS or LPS for 2h. The cells were collected. Total RNA was isolated by Trizol extraction Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany. The library preparation included a polyA enrichment. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: C57BL/6 strain Experimental Factor: wild type genotype genotype Experimental Factor: lipopolysaccharide stimulus ERX1787543 E-MTAB-5190:WT2LPS_p ERP019492 RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice ERS1430340 SAMEA4531161 WT2LPS_p RNA-Seq TRANSCRIPTOMIC RANDOM Spenocytes were stained for CD3- CD19- CD11c+ MHC-II+ CD45+ and sorted to high purity by flow cytometry Isolated cells were plated in D10+ medium, stimulated with either PBS or LPS for 2h. The cells were collected. Total RNA was isolated by Trizol extraction Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany. The library preparation included a polyA enrichment. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: C57BL/6 strain Experimental Factor: wild type genotype genotype Experimental Factor: lipopolysaccharide stimulus ERX1787544 E-MTAB-5190:WT1O_p ERP019492 RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice ERS1430341 SAMEA4531162 WT1O_p RNA-Seq TRANSCRIPTOMIC RANDOM Spenocytes were stained for CD3- CD19- CD11c+ MHC-II+ CD45+ and sorted to high purity by flow cytometry Isolated cells were plated in D10+ medium, stimulated with either PBS or LPS for 2h. The cells were collected. Total RNA was isolated by Trizol extraction Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany. The library preparation included a polyA enrichment. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: C57BL/6 strain Experimental Factor: wild type genotype genotype Experimental Factor: PBS stimulus ERX1787545 E-MTAB-5190:WT2O_p ERP019492 RNA-Seq of steady-state and LPS stimulated splenic classical dendritic cells from wild type, germ-free and IFNAR knockout mice ERS1430342 SAMEA4531163 WT2O_p RNA-Seq TRANSCRIPTOMIC RANDOM Spenocytes were stained for CD3- CD19- CD11c+ MHC-II+ CD45+ and sorted to high purity by flow cytometry Isolated cells were plated in D10+ medium, stimulated with either PBS or LPS for 2h. The cells were collected. Total RNA was isolated by Trizol extraction Libraries were prepared by the Deep Sequencing Unit at the MPI in Freiburg, Germany. The library preparation included a polyA enrichment. 100 0 F Application Read Forward 1 1 R Application Read Reverse 51 Illumina HiSeq 2500 Experimental Factor: C57BL/6 strain Experimental Factor: wild type genotype genotype Experimental Factor: PBS stimulus