ERP134375 PRJEB49850 Molecular_mechanisms_of_haploid_gametic_selection_in_Zebrafish_DNA-sc-6748 Molecular_mechanisms_of_haploid_gametic_selection_in_Zebrafish_DNA An inescapable consequence of sex in eukaryotes is the evolution of a biphasic life cycle with alternation diploid and haploid gametic phases. But while the consequences of natural selection on diploid individuals have been elucidated, the haploid phase has been neglected so far. Make gametes (sperm) are produced in large numbers but only very few reach the fertilisation site. The potential for selection occurring at ejaculate level is therefore strong. There is growing evidence that selection acting on haploid spermatids may drive rapid evolutionary change and play a key role in the maintenance of genetic variation. With the new advances in single cell sequencing, we aim to understand that genes are expressed at post meiotic haploid stage of spermatogenesis and determine which of these genes may be under selection. We aim to further explore the link between sperm phenotype and its underlying genotype. These findings will help us to illuminate the cellular and molecular mechanisms driving selection within an ejaculate. Sperm are produced in large numbers but only very few reach the fertilisation site. The potential of selection occurring at ejaculate level is therefore strong. Employing single cell sequencing will provide us with an understanding of the genes expressed at the post meiotic haploid stage of spermatogenesis. We will further test and investigate which of these genes may be under selection. These findings will help us to illuminate the cellular and molecular mechanisms driving selection within an ejaculate. ENA-FIRST-PUBLIC 2023-09-19 ENA-LAST-UPDATE 2023-09-19