ERX1930086 E-MTAB-5541:Zeb1_Cb192_s ERP021919 ChIP-seq for Zeb1 in Human Neural Stem Cells and Glioblastoma Cancer Stem-like Cells ERS1580348 SAMEA103891180 Zeb1_Cb192_s ChIP-Seq GENOMIC ChIP Cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 Cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011), with an anti-ZEB1 antibody DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol. Illumina HiSeq 2000 Experimental Factor: wild type genotype genotype ERX1930087 E-MTAB-5541:Zeb1_NCH421k_s ERP021919 ChIP-seq for Zeb1 in Human Neural Stem Cells and Glioblastoma Cancer Stem-like Cells ERS1580349 SAMEA103891181 Zeb1_NCH421k_s ChIP-Seq GENOMIC ChIP Cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 Cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011), with an anti-ZEB1 antibody DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol. Illumina HiSeq 2000 Experimental Factor: NCH421k genotype