ERS1580348 SAMEA103891180 9606 Homo sapiens Protocols: Cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 Cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011), with an anti-ZEB1 antibody DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol. ENA-FIRST-PUBLIC 2018-04-24T17:03:46Z ENA-LAST-UPDATE 2017-03-06T12:10:52Z External Id SAMEA103891180 INSDC center name Instituto Gulbenkian de Ciencia INSDC first public 2018-04-24T17:03:46Z INSDC last update 2017-03-06T12:10:52Z INSDC status public Submitter Id E-MTAB-5541:Zeb1_Cb192 broker name ArrayExpress cell line stem cell derived cell line cell type neural stem cell common name human genotype wild type genotype growth condition media sample name E-MTAB-5541:Zeb1_Cb192 scientific_name Homo sapiens ERS1580349 SAMEA103891181 9606 Homo sapiens Protocols: Cells were cultured as previously described (Conti et al. 2005), using laminin as a media supplement (20ug/mL), and plated at a density of 28,000/cm2 Cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011), with an anti-ZEB1 antibody DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol. ENA-FIRST-PUBLIC 2018-04-24T17:03:46Z ENA-LAST-UPDATE 2017-03-06T12:10:54Z External Id SAMEA103891181 INSDC center name Instituto Gulbenkian de Ciencia INSDC first public 2018-04-24T17:03:46Z INSDC last update 2017-03-06T12:10:54Z INSDC status public Submitter Id E-MTAB-5541:Zeb1_NCH421k broker name ArrayExpress cell line D4 glioblastoma derived primary cell line cell type cancer stem-like cell common name human disease glioblastoma multiforme genotype NCH421k growth condition media sample name E-MTAB-5541:Zeb1_NCH421k scientific_name Homo sapiens tumor grading IV