ERP135108 PRJEB50520 ad965922-465e-4a58-ad89-0c6155b600a8 A diverse reference set of cultured SARS-CoV-2 genomes sequenced using various amplification methods and instrument platforms We present a high-quality collection of SARS-CoV-2 whole genome sequences generated from cultured PCR-positive specimens collected in Oxfordshire UK as part of routine national surveillance. Cultured virus RNA was sequenced using Illumina and Oxford Nanopore platforms with both ARTIC and ONT Midnight tiling amplicon protocols, in addition to sequence-independent amplification (SISPA). Accompanying these raw reads are 'truth' variant calls available from https://github.com/iqbal-lab-org/covid-truth-datasets. Represented PANGO lineages include: B, B.1, B.1.1.7, B.1.1.7 (E484K), B.1.214.2, B.1.351, B.1.525, B.1.617.2, B.28, BA.1, P.1, and P.2. We present a high-quality collection of SARS-CoV-2 whole genome sequences generated from cultured PCR-positive specimens collected in Oxfordshire UK as part of routine national surveillance. Cultured virus RNA was sequenced using Illumina and Oxford Nanopore platforms with both ARTIC and ONT Midnight tiling amplicon protocols, in addition to sequence-independent amplification (SISPA). Accompanying these raw reads are 'truth' variant calls available from https://github.com/iqbal-lab-org/covid-truth-datasets. Represented PANGO lineages include: B, B.1, B.1.1.7, B.1.1.7 (E484K), B.1.214.2, B.1.351, B.1.525, B.1.617.2, B.28, BA.1, P.1, and P.2. ENA-FIRST-PUBLIC 2022-02-01 ENA-LAST-UPDATE 2024-01-29