ERX7830442 E-MTAB-11223_2:SHSY5Y-H3K27me3-0d_p ERP135121 PRJEB50532 Whole Genome Bisulfite Sequencing (WGBS) of the SH-SY5Y human neuroblastoma cell line ERS10360347 SAMEA12754100 SHSY5Y-H3K27me3-0d_p Bisulfite-Seq GENOMIC RANDOM Cells were collected at: 7 days of Vincristine treatment (150 nM), 7 days of Vincristine treatment (150 nM) followed by recovery for a further 7 days after the drug removal, or untreated. Genomic DNA was extracted from cells using the Qiagen blood and tissue DNA extraction kit as per manufacturer's instructions. 1 µg of genomic DNA was fragmented to an average size of 300 bp using a Covaris sonicator, and sonicated DNA was used for MethylC-seq library preparation with the NEXTFLEX Bisulfite Library Prep Kit (PerkinElmer, Waltham, MA, USA) and bisulfite treatment with the EZ DNA-methylation Gold Kit (Zymo Research, Irvine, CA, USA) according to manufacturer's instructions. HiSeq X Ten Experimental Factor: treatment none ERX7830443 E-MTAB-11223_2:SHSY5Y-H3K27me3-14d_p ERP135121 PRJEB50532 Whole Genome Bisulfite Sequencing (WGBS) of the SH-SY5Y human neuroblastoma cell line ERS10360348 SAMEA12754101 SHSY5Y-H3K27me3-14d_p Bisulfite-Seq GENOMIC RANDOM Cells were collected at: 7 days of Vincristine treatment (150 nM), 7 days of Vincristine treatment (150 nM) followed by recovery for a further 7 days after the drug removal, or untreated. Genomic DNA was extracted from cells using the Qiagen blood and tissue DNA extraction kit as per manufacturer's instructions. 1 µg of genomic DNA was fragmented to an average size of 300 bp using a Covaris sonicator, and sonicated DNA was used for MethylC-seq library preparation with the NEXTFLEX Bisulfite Library Prep Kit (PerkinElmer, Waltham, MA, USA) and bisulfite treatment with the EZ DNA-methylation Gold Kit (Zymo Research, Irvine, CA, USA) according to manufacturer's instructions. HiSeq X Ten Experimental Factor: treatment 7 days with compound; 7 days recovery ERX7830444 E-MTAB-11223_2:SHSY5Y-H3K27me3-7d_p ERP135121 PRJEB50532 Whole Genome Bisulfite Sequencing (WGBS) of the SH-SY5Y human neuroblastoma cell line ERS10360349 SAMEA12754102 SHSY5Y-H3K27me3-7d_p Bisulfite-Seq GENOMIC RANDOM Cells were collected at: 7 days of Vincristine treatment (150 nM), 7 days of Vincristine treatment (150 nM) followed by recovery for a further 7 days after the drug removal, or untreated. Genomic DNA was extracted from cells using the Qiagen blood and tissue DNA extraction kit as per manufacturer's instructions. 1 µg of genomic DNA was fragmented to an average size of 300 bp using a Covaris sonicator, and sonicated DNA was used for MethylC-seq library preparation with the NEXTFLEX Bisulfite Library Prep Kit (PerkinElmer, Waltham, MA, USA) and bisulfite treatment with the EZ DNA-methylation Gold Kit (Zymo Research, Irvine, CA, USA) according to manufacturer's instructions. HiSeq X Ten Experimental Factor: treatment 7 days with compound