ERS10360347 SAMEA12754100 9606 Homo sapiens Protocols: Cells were collected at: 7 days of Vincristine treatment (150 nM), 7 days of Vincristine treatment (150 nM) followed by recovery for a further 7 days after the drug removal, or untreated. Genomic DNA was extracted from cells using the Qiagen blood and tissue DNA extraction kit as per manufacturer's instructions. 1 µg of genomic DNA was fragmented to an average size of 300 bp using a Covaris sonicator, and sonicated DNA was used for MethylC-seq library preparation with the NEXTFLEX Bisulfite Library Prep Kit (PerkinElmer, Waltham, MA, USA) and bisulfite treatment with the EZ DNA-methylation Gold Kit (Zymo Research, Irvine, CA, USA) according to manufacturer's instructions. ENA-FIRST-PUBLIC 2022-10-01 ENA-LAST-UPDATE 2022-10-01 External Id SAMEA12754100 INSDC center alias Garvan Institute of Medical Research INSDC center name Garvan Institute of Medical Research INSDC first public 2022-10-01T16:25:38Z INSDC last update 2022-10-01T16:25:38Z INSDC status public Submitter Id E-MTAB-11223_2:SHSY5Y-H3K27me3-0d age 4 broker name ArrayExpress cell_line SH-SY5Y common name human developmental stage juvenile stage disease neuroblastoma dose 0 organism part bone marrow sample name E-MTAB-11223_2:SHSY5Y-H3K27me3-0d sex female ERS10360348 SAMEA12754101 9606 Homo sapiens Protocols: Cells were collected at: 7 days of Vincristine treatment (150 nM), 7 days of Vincristine treatment (150 nM) followed by recovery for a further 7 days after the drug removal, or untreated. Genomic DNA was extracted from cells using the Qiagen blood and tissue DNA extraction kit as per manufacturer's instructions. 1 µg of genomic DNA was fragmented to an average size of 300 bp using a Covaris sonicator, and sonicated DNA was used for MethylC-seq library preparation with the NEXTFLEX Bisulfite Library Prep Kit (PerkinElmer, Waltham, MA, USA) and bisulfite treatment with the EZ DNA-methylation Gold Kit (Zymo Research, Irvine, CA, USA) according to manufacturer's instructions. ENA-FIRST-PUBLIC 2022-10-01 ENA-LAST-UPDATE 2022-10-01 External Id SAMEA12754101 INSDC center alias Garvan Institute of Medical Research INSDC center name Garvan Institute of Medical Research INSDC first public 2022-10-01T16:25:38Z INSDC last update 2022-10-01T16:25:38Z INSDC status public Submitter Id E-MTAB-11223_2:SHSY5Y-H3K27me3-14d age 4 broker name ArrayExpress cell_line SH-SY5Y common name human compound vincristine developmental stage juvenile stage disease neuroblastoma dose 150 organism part bone marrow sample name E-MTAB-11223_2:SHSY5Y-H3K27me3-14d sex female treatment 7 days with compound; 7 days recovery ERS10360349 SAMEA12754102 9606 Homo sapiens Protocols: Cells were collected at: 7 days of Vincristine treatment (150 nM), 7 days of Vincristine treatment (150 nM) followed by recovery for a further 7 days after the drug removal, or untreated. Genomic DNA was extracted from cells using the Qiagen blood and tissue DNA extraction kit as per manufacturer's instructions. 1 µg of genomic DNA was fragmented to an average size of 300 bp using a Covaris sonicator, and sonicated DNA was used for MethylC-seq library preparation with the NEXTFLEX Bisulfite Library Prep Kit (PerkinElmer, Waltham, MA, USA) and bisulfite treatment with the EZ DNA-methylation Gold Kit (Zymo Research, Irvine, CA, USA) according to manufacturer's instructions. ENA-FIRST-PUBLIC 2022-10-01 ENA-LAST-UPDATE 2022-10-01 External Id SAMEA12754102 INSDC center alias Garvan Institute of Medical Research INSDC center name Garvan Institute of Medical Research INSDC first public 2022-10-01T16:25:38Z INSDC last update 2022-10-01T16:25:38Z INSDC status public Submitter Id E-MTAB-11223_2:SHSY5Y-H3K27me3-7d age 4 broker name ArrayExpress cell_line SH-SY5Y common name human compound vincristine developmental stage juvenile stage disease neuroblastoma dose 150 organism part bone marrow sample name E-MTAB-11223_2:SHSY5Y-H3K27me3-7d sex female treatment 7 days with compound