ERS10419850 SAMEA12812889 10090 Mus musculus house mouse Protocols: Cells were washed with PBS 1x and the lysed with the lysis buffer provided by the manufacturer of the kit. Cells were harvested from the bone marrow of 2 femurs from 6-week old mice in the C57Bl6 background, wild type, in alpha-MEM. After centrifugation at 1000 rpm for 5 min, the supernatant was removed and the red cells were lyzed using 4,5mL of water. 500uL of PBS 10x was added to equilibrate the pH and osmolarity, and the cells were centrifuged again at 1000 rpm for 5 min. The cells were resuspended in alpha-MEM containing 10% FBS and 30ng/mL of murine MCSF (R&D sytems) and seeded in suspension culture dishes (Corning) for 3 days. Cells described in the growth protocol were harvested from the plate using a cell scraper and seeded at the density of 5,000 cells/well in 96-well culture dishes. The cells were grown in alpha-MEM containing 10%FBS, antibiotics, and 30ng/mL MCSF RNA was extracted using RNAeasy Mini kit following manufacturer’s instructions (Cat no. 74104 - Qiagen) Illumina TruSeq Stranded mRNA strain C57BL/6 isolate not applicable ENA-FIRST-PUBLIC 2024-02-02T00:44:02Z organism Mus musculus organism Mus musculus ENA-LAST-UPDATE 2024-02-02T00:44:02Z progenitor cell type bone marrow macrophage scientific_name Mus musculus common name house mouse organism part bone marrow cell_type bone marrow macrophage age 6 week genotype wild type genotype ENA-FIRST-PUBLIC 2024-02-02 ENA-LAST-UPDATE 2024-02-02 ERS10419848 SAMEA12812887 10090 Mus musculus house mouse Protocols: Cells were washed with PBS 1x and the lysed with the lysis buffer provided by the manufacturer of the kit. Cells were harvested from the bone marrow of 2 femurs from 6-week old mice in the C57Bl6 background, expressing GFP under regulation of Ibsp and mCherry under regulation of Acp5. Due to the insertion of mCherry, the Acp5 gene product was alrered, rendering a TRAP deficient mouse strain. Cells were harvested in alpha MEM. After centrifugation at 1000 rpm for 5 min, the supernatant was removed and the red cells were lyzed using 4,5mL of water. 500uL of PBS 10x was added to equilibrate the pH and osmolarity, and the cells were centrifuged again at 1000 rpm for 5 min. The cells were resuspended in alpha-MEM containing 10% FBS and 30ng/mL of murine MCSF (R&D sytems) and seeded in suspension culture dishes (Corning) for 3 days. Cells described in the growth protocol were harvested from the plate using a cell scraper and seeded at the density of 5,000 cells/well in 96-well culture dishes. The cells were grown in alpha-MEM containing 10%FBS, antibiotics, and 30ng/mL MCSF RNA was extracted using RNAeasy Mini kit following manufacturer’s instructions (Cat no. 74104 - Qiagen) Illumina TruSeq Stranded mRNA strain C57BL/6 isolate not applicable ENA-FIRST-PUBLIC 2024-02-02T00:44:03Z organism Mus musculus organism Mus musculus ENA-LAST-UPDATE 2024-02-02T00:44:03Z progenitor cell type bone marrow macrophage scientific_name Mus musculus common name house mouse organism part bone marrow cell_type bone marrow macrophage age 6 week genotype TRAP knockout ENA-FIRST-PUBLIC 2024-02-02 ENA-LAST-UPDATE 2024-02-02 ERS10419845 SAMEA12812884 10090 Mus musculus house mouse Protocols: Cells were washed with PBS 1x and the lysed with the lysis buffer provided by the manufacturer of the kit. Cells were harvested from the bone marrow of 2 femurs from 6-week old mice in the C57Bl6 background, expressing GFP under regulation of Ibsp and mCherry under regulation of Acp5. Due to the insertion of mCherry, the Acp5 gene product was alrered, rendering a TRAP deficient mouse strain. Cells were harvested in alpha MEM. After centrifugation at 1000 rpm for 5 min, the supernatant was removed and the red cells were lyzed using 4,5mL of water. 500uL of PBS 10x was added to equilibrate the pH and osmolarity, and the cells were centrifuged again at 1000 rpm for 5 min. The cells were resuspended in alpha-MEM containing 10% FBS and 30ng/mL of murine MCSF (R&D sytems) and seeded in suspension culture dishes (Corning) for 3 days. Cells described in the growth protocol were harvested from the plate using a cell scraper and seeded at the density of 5,000 cells/well in 96-well culture dishes. The cells were grown in alpha-MEM containing 10%FBS, antibiotics, 30ng/mL MCSF and 4ng/mL RANKL (R&D systems) RNA was extracted using RNAeasy Mini kit following manufacturer’s instructions (Cat no. 74104 - Qiagen) Illumina TruSeq Stranded mRNA strain C57BL/6 isolate not applicable ENA-FIRST-PUBLIC 2024-02-02T00:44:03Z organism Mus musculus organism Mus musculus ENA-LAST-UPDATE 2024-02-02T00:44:03Z progenitor cell type bone marrow macrophage scientific_name Mus musculus common name house mouse organism part bone marrow cell_type osteoclast age 6 week genotype TRAP knockout ENA-FIRST-PUBLIC 2024-02-02 ENA-LAST-UPDATE 2024-02-02 ERS10419851 SAMEA12812890 10090 Mus musculus house mouse Protocols: Cells were washed with PBS 1x and the lysed with the lysis buffer provided by the manufacturer of the kit. Cells were harvested from the bone marrow of 2 femurs from 6-week old mice in the C57Bl6 background, wild type, in alpha-MEM. After centrifugation at 1000 rpm for 5 min, the supernatant was removed and the red cells were lyzed using 4,5mL of water. 500uL of PBS 10x was added to equilibrate the pH and osmolarity, and the cells were centrifuged again at 1000 rpm for 5 min. The cells were resuspended in alpha-MEM containing 10% FBS and 30ng/mL of murine MCSF (R&D sytems) and seeded in suspension culture dishes (Corning) for 3 days. Cells described in the growth protocol were harvested from the plate using a cell scraper and seeded at the density of 5,000 cells/well in 96-well culture dishes. The cells were grown in alpha-MEM containing 10%FBS, antibiotics, and 30ng/mL MCSF RNA was extracted using RNAeasy Mini kit following manufacturer’s instructions (Cat no. 74104 - Qiagen) Illumina TruSeq Stranded mRNA strain C57BL/6 isolate not applicable ENA-FIRST-PUBLIC 2024-02-02T00:44:02Z organism Mus musculus organism Mus musculus ENA-LAST-UPDATE 2024-02-02T00:44:02Z progenitor cell type bone marrow macrophage scientific_name Mus musculus common name house mouse organism part bone marrow cell_type bone marrow macrophage age 6 week genotype wild type genotype ENA-FIRST-PUBLIC 2024-02-02 ENA-LAST-UPDATE 2024-02-02 ERS10419852 SAMEA12812891 10090 Mus musculus house mouse Protocols: Cells were washed with PBS 1x and the lysed with the lysis buffer provided by the manufacturer of the kit. Cells were harvested from the bone marrow of 2 femurs from 6-week old mice in the C57Bl6 background, wild type, in alpha-MEM. After centrifugation at 1000 rpm for 5 min, the supernatant was removed and the red cells were lyzed using 4,5mL of water. 500uL of PBS 10x was added to equilibrate the pH and osmolarity, and the cells were centrifuged again at 1000 rpm for 5 min. The cells were resuspended in alpha-MEM containing 10% FBS and 30ng/mL of murine MCSF (R&D sytems) and seeded in suspension culture dishes (Corning) for 3 days. Cells described in the growth protocol were harvested from the plate using a cell scraper and seeded at the density of 5,000 cells/well in 96-well culture dishes. The cells were grown in alpha-MEM containing 10%FBS, antibiotics, and 30ng/mL MCSF RNA was extracted using RNAeasy Mini kit following manufacturer’s instructions (Cat no. 74104 - Qiagen) Illumina TruSeq Stranded mRNA strain C57BL/6 isolate not applicable ENA-FIRST-PUBLIC 2024-02-02T00:44:02Z organism Mus musculus organism Mus musculus ENA-LAST-UPDATE 2024-02-02T00:44:02Z progenitor cell type bone marrow macrophage scientific_name Mus musculus common name house mouse organism part bone marrow cell_type bone marrow macrophage age 6 week genotype wild type genotype ENA-FIRST-PUBLIC 2024-02-02 ENA-LAST-UPDATE 2024-02-02 ERS10419853 SAMEA12812892 10090 Mus musculus house mouse Protocols: Cells were washed with PBS 1x and the lysed with the lysis buffer provided by the manufacturer of the kit. Cells were harvested from the bone marrow of 2 femurs from 6-week old mice in the C57Bl6 background, wild type, in alpha-MEM. After centrifugation at 1000 rpm for 5 min, the supernatant was removed and the red cells were lyzed using 4,5mL of water. 500uL of PBS 10x was added to equilibrate the pH and osmolarity, and the cells were centrifuged again at 1000 rpm for 5 min. The cells were resuspended in alpha-MEM containing 10% FBS and 30ng/mL of murine MCSF (R&D sytems) and seeded in suspension culture dishes (Corning) for 3 days. Cells described in the growth protocol were harvested from the plate using a cell scraper and seeded at the density of 5,000 cells/well in 96-well culture dishes. The cells were grown in alpha-MEM containing 10%FBS, antibiotics, 30ng/mL MCSF and 4ng/mL RANKL (R&D systems) RNA was extracted using RNAeasy Mini kit following manufacturer’s instructions (Cat no. 74104 - Qiagen) Illumina TruSeq Stranded mRNA strain C57BL/6 isolate not applicable ENA-FIRST-PUBLIC 2024-02-02T00:44:02Z organism Mus musculus organism Mus musculus ENA-LAST-UPDATE 2024-02-02T00:44:02Z progenitor cell type bone marrow macrophage scientific_name Mus musculus common name house mouse organism part bone marrow cell_type osteoclast age 6 week genotype wild type genotype ENA-FIRST-PUBLIC 2024-02-02 ENA-LAST-UPDATE 2024-02-02 ERS10419847 SAMEA12812886 10090 Mus musculus house mouse Protocols: Cells were washed with PBS 1x and the lysed with the lysis buffer provided by the manufacturer of the kit. Cells were harvested from the bone marrow of 2 femurs from 6-week old mice in the C57Bl6 background, expressing GFP under regulation of Ibsp and mCherry under regulation of Acp5. Due to the insertion of mCherry, the Acp5 gene product was alrered, rendering a TRAP deficient mouse strain. Cells were harvested in alpha MEM. After centrifugation at 1000 rpm for 5 min, the supernatant was removed and the red cells were lyzed using 4,5mL of water. 500uL of PBS 10x was added to equilibrate the pH and osmolarity, and the cells were centrifuged again at 1000 rpm for 5 min. The cells were resuspended in alpha-MEM containing 10% FBS and 30ng/mL of murine MCSF (R&D sytems) and seeded in suspension culture dishes (Corning) for 3 days. Cells described in the growth protocol were harvested from the plate using a cell scraper and seeded at the density of 5,000 cells/well in 96-well culture dishes. The cells were grown in alpha-MEM containing 10%FBS, antibiotics, and 30ng/mL MCSF RNA was extracted using RNAeasy Mini kit following manufacturer’s instructions (Cat no. 74104 - Qiagen) Illumina TruSeq Stranded mRNA strain C57BL/6 isolate not applicable ENA-FIRST-PUBLIC 2024-02-02T00:44:03Z organism Mus musculus organism Mus musculus ENA-LAST-UPDATE 2024-02-02T00:44:03Z progenitor cell type bone marrow macrophage scientific_name Mus musculus common name house mouse organism part bone marrow cell_type bone marrow macrophage age 6 week genotype TRAP knockout ENA-FIRST-PUBLIC 2024-02-02 ENA-LAST-UPDATE 2024-02-02 ERS10419846 SAMEA12812885 10090 Mus musculus house mouse Protocols: Cells were washed with PBS 1x and the lysed with the lysis buffer provided by the manufacturer of the kit. Cells were harvested from the bone marrow of 2 femurs from 6-week old mice in the C57Bl6 background, expressing GFP under regulation of Ibsp and mCherry under regulation of Acp5. Due to the insertion of mCherry, the Acp5 gene product was alrered, rendering a TRAP deficient mouse strain. Cells were harvested in alpha MEM. After centrifugation at 1000 rpm for 5 min, the supernatant was removed and the red cells were lyzed using 4,5mL of water. 500uL of PBS 10x was added to equilibrate the pH and osmolarity, and the cells were centrifuged again at 1000 rpm for 5 min. The cells were resuspended in alpha-MEM containing 10% FBS and 30ng/mL of murine MCSF (R&D sytems) and seeded in suspension culture dishes (Corning) for 3 days. Cells described in the growth protocol were harvested from the plate using a cell scraper and seeded at the density of 5,000 cells/well in 96-well culture dishes. The cells were grown in alpha-MEM containing 10%FBS, antibiotics, 30ng/mL MCSF and 4ng/mL RANKL (R&D systems) RNA was extracted using RNAeasy Mini kit following manufacturer’s instructions (Cat no. 74104 - Qiagen) Illumina TruSeq Stranded mRNA strain C57BL/6 isolate not applicable ENA-FIRST-PUBLIC 2024-02-02T00:44:03Z organism Mus musculus organism Mus musculus ENA-LAST-UPDATE 2024-02-02T00:44:03Z progenitor cell type bone marrow macrophage scientific_name Mus musculus common name house mouse organism part bone marrow cell_type osteoclast age 6 week genotype TRAP knockout ENA-FIRST-PUBLIC 2024-02-02 ENA-LAST-UPDATE 2024-02-02 ERS10419854 SAMEA12812893 10090 Mus musculus house mouse Protocols: Cells were washed with PBS 1x and the lysed with the lysis buffer provided by the manufacturer of the kit. Cells were harvested from the bone marrow of 2 femurs from 6-week old mice in the C57Bl6 background, wild type, in alpha-MEM. After centrifugation at 1000 rpm for 5 min, the supernatant was removed and the red cells were lyzed using 4,5mL of water. 500uL of PBS 10x was added to equilibrate the pH and osmolarity, and the cells were centrifuged again at 1000 rpm for 5 min. The cells were resuspended in alpha-MEM containing 10% FBS and 30ng/mL of murine MCSF (R&D sytems) and seeded in suspension culture dishes (Corning) for 3 days. Cells described in the growth protocol were harvested from the plate using a cell scraper and seeded at the density of 5,000 cells/well in 96-well culture dishes. The cells were grown in alpha-MEM containing 10%FBS, antibiotics, 30ng/mL MCSF and 4ng/mL RANKL (R&D systems) RNA was extracted using RNAeasy Mini kit following manufacturer’s instructions (Cat no. 74104 - Qiagen) Illumina TruSeq Stranded mRNA strain C57BL/6 isolate not applicable ENA-FIRST-PUBLIC 2024-02-02T00:44:02Z organism Mus musculus organism Mus musculus ENA-LAST-UPDATE 2024-02-02T00:44:02Z progenitor cell type bone marrow macrophage scientific_name Mus musculus common name house mouse organism part bone marrow cell_type osteoclast age 6 week genotype wild type genotype ENA-FIRST-PUBLIC 2024-02-02 ENA-LAST-UPDATE 2024-02-02 ERS10419844 SAMEA12812883 10090 Mus musculus house mouse Protocols: Cells were washed with PBS 1x and the lysed with the lysis buffer provided by the manufacturer of the kit. Cells were harvested from the bone marrow of 2 femurs from 6-week old mice in the C57Bl6 background, expressing GFP under regulation of Ibsp and mCherry under regulation of Acp5. Due to the insertion of mCherry, the Acp5 gene product was alrered, rendering a TRAP deficient mouse strain. Cells were harvested in alpha MEM. After centrifugation at 1000 rpm for 5 min, the supernatant was removed and the red cells were lyzed using 4,5mL of water. 500uL of PBS 10x was added to equilibrate the pH and osmolarity, and the cells were centrifuged again at 1000 rpm for 5 min. The cells were resuspended in alpha-MEM containing 10% FBS and 30ng/mL of murine MCSF (R&D sytems) and seeded in suspension culture dishes (Corning) for 3 days. Cells described in the growth protocol were harvested from the plate using a cell scraper and seeded at the density of 5,000 cells/well in 96-well culture dishes. The cells were grown in alpha-MEM containing 10%FBS, antibiotics, 30ng/mL MCSF and 4ng/mL RANKL (R&D systems) RNA was extracted using RNAeasy Mini kit following manufacturer’s instructions (Cat no. 74104 - Qiagen) Illumina TruSeq Stranded mRNA strain C57BL/6 isolate not applicable ENA-FIRST-PUBLIC 2024-02-02T00:44:03Z organism Mus musculus organism Mus musculus ENA-LAST-UPDATE 2024-02-02T00:44:03Z progenitor cell type bone marrow macrophage scientific_name Mus musculus common name house mouse organism part bone marrow cell_type osteoclast age 6 week genotype TRAP knockout ENA-FIRST-PUBLIC 2024-02-02 ENA-LAST-UPDATE 2024-02-02 ERS10419855 SAMEA12812894 10090 Mus musculus house mouse Protocols: Cells were washed with PBS 1x and the lysed with the lysis buffer provided by the manufacturer of the kit. Cells were harvested from the bone marrow of 2 femurs from 6-week old mice in the C57Bl6 background, wild type, in alpha-MEM. After centrifugation at 1000 rpm for 5 min, the supernatant was removed and the red cells were lyzed using 4,5mL of water. 500uL of PBS 10x was added to equilibrate the pH and osmolarity, and the cells were centrifuged again at 1000 rpm for 5 min. The cells were resuspended in alpha-MEM containing 10% FBS and 30ng/mL of murine MCSF (R&D sytems) and seeded in suspension culture dishes (Corning) for 3 days. Cells described in the growth protocol were harvested from the plate using a cell scraper and seeded at the density of 5,000 cells/well in 96-well culture dishes. The cells were grown in alpha-MEM containing 10%FBS, antibiotics, 30ng/mL MCSF and 4ng/mL RANKL (R&D systems) RNA was extracted using RNAeasy Mini kit following manufacturer’s instructions (Cat no. 74104 - Qiagen) Illumina TruSeq Stranded mRNA strain C57BL/6 isolate not applicable ENA-FIRST-PUBLIC 2024-02-02T00:44:02Z organism Mus musculus organism Mus musculus ENA-LAST-UPDATE 2024-02-02T00:44:02Z progenitor cell type bone marrow macrophage scientific_name Mus musculus common name house mouse organism part bone marrow cell_type osteoclast age 6 week genotype wild type genotype ENA-FIRST-PUBLIC 2024-02-02 ENA-LAST-UPDATE 2024-02-02 ERS10419849 SAMEA12812888 10090 Mus musculus house mouse Protocols: Cells were washed with PBS 1x and the lysed with the lysis buffer provided by the manufacturer of the kit. Cells were harvested from the bone marrow of 2 femurs from 6-week old mice in the C57Bl6 background, expressing GFP under regulation of Ibsp and mCherry under regulation of Acp5. Due to the insertion of mCherry, the Acp5 gene product was alrered, rendering a TRAP deficient mouse strain. Cells were harvested in alpha MEM. After centrifugation at 1000 rpm for 5 min, the supernatant was removed and the red cells were lyzed using 4,5mL of water. 500uL of PBS 10x was added to equilibrate the pH and osmolarity, and the cells were centrifuged again at 1000 rpm for 5 min. The cells were resuspended in alpha-MEM containing 10% FBS and 30ng/mL of murine MCSF (R&D sytems) and seeded in suspension culture dishes (Corning) for 3 days. Cells described in the growth protocol were harvested from the plate using a cell scraper and seeded at the density of 5,000 cells/well in 96-well culture dishes. The cells were grown in alpha-MEM containing 10%FBS, antibiotics, and 30ng/mL MCSF RNA was extracted using RNAeasy Mini kit following manufacturer’s instructions (Cat no. 74104 - Qiagen) Illumina TruSeq Stranded mRNA strain C57BL/6 isolate not applicable ENA-FIRST-PUBLIC 2024-02-02T00:44:02Z organism Mus musculus organism Mus musculus ENA-LAST-UPDATE 2024-02-02T00:44:02Z progenitor cell type bone marrow macrophage scientific_name Mus musculus common name house mouse organism part bone marrow cell_type bone marrow macrophage age 6 week genotype TRAP knockout ENA-FIRST-PUBLIC 2024-02-02 ENA-LAST-UPDATE 2024-02-02