ERX7881755 E-MTAB-11409:Sample 10_s ERP135203 PRJEB50607 Flexible wood caused by altered lignin, following viral-induced suppression of phenylalanine ammonia lyase (PAL) activity. sRNA-seq ERS10420656 SAMEA12813696 Sample 10_s miRNA-Seq TRANSCRIPTOMIC RT-PCR RNA was extracted from 10 mg of stem material. 6 uninfected and 6 infected graft segments were used. After removing the bark, whole xylem segments were flash frozen in liquid nitrogen. Newly differentiating xylem was scraped from frozen xylem segments and then ground into a fine powder using a mortar and pestle with liquid nitrogen. Apple stem material was propagated from 2 uninfected and 2 ARW-infected Lord Lambourne apple trees, which was provided by the East Malling Research Station, Kent, UK in 1996. Budwood was grafted onto M27 rootstocks using the 'whip and tongue' grafting method (Bradley & Garner, 2013) in April 2017. Grafts were kept in a glass house at 22°C and one-year old stem material was harvested the following year for analysis. Qiagen RNAeasy Plant Power kit to extract RNA from ground xylem samples. DNA was removed with the Qiagen DNAse Max kit. NEBNext Small RNA Library Prep NextSeq 550 Experimental Factor: infect apple rubbery wood virus 1 ERX7881756 E-MTAB-11409:Sample 6_s ERP135203 PRJEB50607 Flexible wood caused by altered lignin, following viral-induced suppression of phenylalanine ammonia lyase (PAL) activity. sRNA-seq ERS10420657 SAMEA12813697 Sample 6_s miRNA-Seq TRANSCRIPTOMIC RT-PCR RNA was extracted from 10 mg of stem material. 6 uninfected and 6 infected graft segments were used. After removing the bark, whole xylem segments were flash frozen in liquid nitrogen. Newly differentiating xylem was scraped from frozen xylem segments and then ground into a fine powder using a mortar and pestle with liquid nitrogen. Apple stem material was propagated from 2 uninfected and 2 ARW-infected Lord Lambourne apple trees, which was provided by the East Malling Research Station, Kent, UK in 1996. Budwood was grafted onto M27 rootstocks using the 'whip and tongue' grafting method (Bradley & Garner, 2013) in April 2017. Grafts were kept in a glass house at 22°C and one-year old stem material was harvested the following year for analysis. Qiagen RNAeasy Plant Power kit to extract RNA from ground xylem samples. DNA was removed with the Qiagen DNAse Max kit. NEBNext Small RNA Library Prep NextSeq 550 Experimental Factor: infect apple rubbery wood virus 1 ERX7881757 E-MTAB-11409:Sample 7_s ERP135203 PRJEB50607 Flexible wood caused by altered lignin, following viral-induced suppression of phenylalanine ammonia lyase (PAL) activity. sRNA-seq ERS10420658 SAMEA12813698 Sample 7_s miRNA-Seq TRANSCRIPTOMIC RT-PCR RNA was extracted from 10 mg of stem material. 6 uninfected and 6 infected graft segments were used. After removing the bark, whole xylem segments were flash frozen in liquid nitrogen. Newly differentiating xylem was scraped from frozen xylem segments and then ground into a fine powder using a mortar and pestle with liquid nitrogen. Apple stem material was propagated from 2 uninfected and 2 ARW-infected Lord Lambourne apple trees, which was provided by the East Malling Research Station, Kent, UK in 1996. Budwood was grafted onto M27 rootstocks using the 'whip and tongue' grafting method (Bradley & Garner, 2013) in April 2017. Grafts were kept in a glass house at 22°C and one-year old stem material was harvested the following year for analysis. Qiagen RNAeasy Plant Power kit to extract RNA from ground xylem samples. DNA was removed with the Qiagen DNAse Max kit. NEBNext Small RNA Library Prep NextSeq 550 Experimental Factor: infect apple rubbery wood virus 1 ERX7881758 E-MTAB-11409:Sample 8_s ERP135203 PRJEB50607 Flexible wood caused by altered lignin, following viral-induced suppression of phenylalanine ammonia lyase (PAL) activity. sRNA-seq ERS10420659 SAMEA12813699 Sample 8_s miRNA-Seq TRANSCRIPTOMIC RT-PCR RNA was extracted from 10 mg of stem material. 6 uninfected and 6 infected graft segments were used. After removing the bark, whole xylem segments were flash frozen in liquid nitrogen. Newly differentiating xylem was scraped from frozen xylem segments and then ground into a fine powder using a mortar and pestle with liquid nitrogen. Apple stem material was propagated from 2 uninfected and 2 ARW-infected Lord Lambourne apple trees, which was provided by the East Malling Research Station, Kent, UK in 1996. Budwood was grafted onto M27 rootstocks using the 'whip and tongue' grafting method (Bradley & Garner, 2013) in April 2017. Grafts were kept in a glass house at 22°C and one-year old stem material was harvested the following year for analysis. Qiagen RNAeasy Plant Power kit to extract RNA from ground xylem samples. DNA was removed with the Qiagen DNAse Max kit. NEBNext Small RNA Library Prep NextSeq 550 Experimental Factor: infect apple rubbery wood virus 1 ERX7881759 E-MTAB-11409:Sample 1_s ERP135203 PRJEB50607 Flexible wood caused by altered lignin, following viral-induced suppression of phenylalanine ammonia lyase (PAL) activity. sRNA-seq ERS10420660 SAMEA12813700 Sample 1_s miRNA-Seq TRANSCRIPTOMIC RT-PCR RNA was extracted from 10 mg of stem material. 6 uninfected and 6 infected graft segments were used. After removing the bark, whole xylem segments were flash frozen in liquid nitrogen. Newly differentiating xylem was scraped from frozen xylem segments and then ground into a fine powder using a mortar and pestle with liquid nitrogen. Apple stem material was propagated from 2 uninfected and 2 ARW-infected Lord Lambourne apple trees, which was provided by the East Malling Research Station, Kent, UK in 1996. Budwood was grafted onto M27 rootstocks using the 'whip and tongue' grafting method (Bradley & Garner, 2013) in April 2017. Grafts were kept in a glass house at 22°C and one-year old stem material was harvested the following year for analysis. Qiagen RNAeasy Plant Power kit to extract RNA from ground xylem samples. DNA was removed with the Qiagen DNAse Max kit. NEBNext Small RNA Library Prep NextSeq 550 Experimental Factor: infect none ERX7881760 E-MTAB-11409:Sample 2_s ERP135203 PRJEB50607 Flexible wood caused by altered lignin, following viral-induced suppression of phenylalanine ammonia lyase (PAL) activity. sRNA-seq ERS10420661 SAMEA12813701 Sample 2_s miRNA-Seq TRANSCRIPTOMIC RT-PCR RNA was extracted from 10 mg of stem material. 6 uninfected and 6 infected graft segments were used. After removing the bark, whole xylem segments were flash frozen in liquid nitrogen. Newly differentiating xylem was scraped from frozen xylem segments and then ground into a fine powder using a mortar and pestle with liquid nitrogen. Apple stem material was propagated from 2 uninfected and 2 ARW-infected Lord Lambourne apple trees, which was provided by the East Malling Research Station, Kent, UK in 1996. Budwood was grafted onto M27 rootstocks using the 'whip and tongue' grafting method (Bradley & Garner, 2013) in April 2017. Grafts were kept in a glass house at 22°C and one-year old stem material was harvested the following year for analysis. Qiagen RNAeasy Plant Power kit to extract RNA from ground xylem samples. DNA was removed with the Qiagen DNAse Max kit. NEBNext Small RNA Library Prep NextSeq 550 Experimental Factor: infect none ERX7881761 E-MTAB-11409:Sample 3_s ERP135203 PRJEB50607 Flexible wood caused by altered lignin, following viral-induced suppression of phenylalanine ammonia lyase (PAL) activity. sRNA-seq ERS10420662 SAMEA12813702 Sample 3_s miRNA-Seq TRANSCRIPTOMIC RT-PCR RNA was extracted from 10 mg of stem material. 6 uninfected and 6 infected graft segments were used. After removing the bark, whole xylem segments were flash frozen in liquid nitrogen. Newly differentiating xylem was scraped from frozen xylem segments and then ground into a fine powder using a mortar and pestle with liquid nitrogen. Apple stem material was propagated from 2 uninfected and 2 ARW-infected Lord Lambourne apple trees, which was provided by the East Malling Research Station, Kent, UK in 1996. Budwood was grafted onto M27 rootstocks using the 'whip and tongue' grafting method (Bradley & Garner, 2013) in April 2017. Grafts were kept in a glass house at 22°C and one-year old stem material was harvested the following year for analysis. Qiagen RNAeasy Plant Power kit to extract RNA from ground xylem samples. DNA was removed with the Qiagen DNAse Max kit. NEBNext Small RNA Library Prep NextSeq 550 Experimental Factor: infect none ERX7881762 E-MTAB-11409:Sample 4_s ERP135203 PRJEB50607 Flexible wood caused by altered lignin, following viral-induced suppression of phenylalanine ammonia lyase (PAL) activity. sRNA-seq ERS10420663 SAMEA12813703 Sample 4_s miRNA-Seq TRANSCRIPTOMIC RT-PCR RNA was extracted from 10 mg of stem material. 6 uninfected and 6 infected graft segments were used. After removing the bark, whole xylem segments were flash frozen in liquid nitrogen. Newly differentiating xylem was scraped from frozen xylem segments and then ground into a fine powder using a mortar and pestle with liquid nitrogen. Apple stem material was propagated from 2 uninfected and 2 ARW-infected Lord Lambourne apple trees, which was provided by the East Malling Research Station, Kent, UK in 1996. Budwood was grafted onto M27 rootstocks using the 'whip and tongue' grafting method (Bradley & Garner, 2013) in April 2017. Grafts were kept in a glass house at 22°C and one-year old stem material was harvested the following year for analysis. Qiagen RNAeasy Plant Power kit to extract RNA from ground xylem samples. DNA was removed with the Qiagen DNAse Max kit. NEBNext Small RNA Library Prep NextSeq 550 Experimental Factor: infect none ERX7881763 E-MTAB-11409:Sample 5_s ERP135203 PRJEB50607 Flexible wood caused by altered lignin, following viral-induced suppression of phenylalanine ammonia lyase (PAL) activity. sRNA-seq ERS10420664 SAMEA12813704 Sample 5_s miRNA-Seq TRANSCRIPTOMIC RT-PCR RNA was extracted from 10 mg of stem material. 6 uninfected and 6 infected graft segments were used. After removing the bark, whole xylem segments were flash frozen in liquid nitrogen. Newly differentiating xylem was scraped from frozen xylem segments and then ground into a fine powder using a mortar and pestle with liquid nitrogen. Apple stem material was propagated from 2 uninfected and 2 ARW-infected Lord Lambourne apple trees, which was provided by the East Malling Research Station, Kent, UK in 1996. Budwood was grafted onto M27 rootstocks using the 'whip and tongue' grafting method (Bradley & Garner, 2013) in April 2017. Grafts were kept in a glass house at 22°C and one-year old stem material was harvested the following year for analysis. Qiagen RNAeasy Plant Power kit to extract RNA from ground xylem samples. DNA was removed with the Qiagen DNAse Max kit. NEBNext Small RNA Library Prep NextSeq 550 Experimental Factor: infect none ERX7881764 E-MTAB-11409:Sample 9_s ERP135203 PRJEB50607 Flexible wood caused by altered lignin, following viral-induced suppression of phenylalanine ammonia lyase (PAL) activity. sRNA-seq ERS10420665 SAMEA12813705 Sample 9_s miRNA-Seq TRANSCRIPTOMIC RT-PCR RNA was extracted from 10 mg of stem material. 6 uninfected and 6 infected graft segments were used. After removing the bark, whole xylem segments were flash frozen in liquid nitrogen. Newly differentiating xylem was scraped from frozen xylem segments and then ground into a fine powder using a mortar and pestle with liquid nitrogen. Apple stem material was propagated from 2 uninfected and 2 ARW-infected Lord Lambourne apple trees, which was provided by the East Malling Research Station, Kent, UK in 1996. Budwood was grafted onto M27 rootstocks using the 'whip and tongue' grafting method (Bradley & Garner, 2013) in April 2017. Grafts were kept in a glass house at 22°C and one-year old stem material was harvested the following year for analysis. Qiagen RNAeasy Plant Power kit to extract RNA from ground xylem samples. DNA was removed with the Qiagen DNAse Max kit. NEBNext Small RNA Library Prep NextSeq 550 Experimental Factor: infect none