ERS10540164 SAMEA12941371 9606 Homo sapiens Protocols: Organoids were collected in 1.5ml Eppendorff tubes and washed three times in 1xPBS. The supernatant of the final wash was discarded and organoids were shock frozen on dry ice and stored at -80C. Total RNA was extracted using the QIAGEN RNeasy Plus Micro kit as per manufacturer's instructions. RNA was eluated in 14 μl of RNAse free water. Libraries were prepared from 250ng of each total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760) and the Poly-A mRNA magnetic isolation module (NEB #E7490) according to the provided protocol ENA first public 2022-06-06 ENA last update 2022-06-06 External Id SAMEA12941371 INSDC center alias Centre for Discovery Brain Sciences University of Edinburgh INSDC center name Centre for Discovery Brain Sciences University of Edinburgh INSDC first public 2022-06-06T12:23:35Z INSDC last update 2022-06-06T12:23:35Z INSDC status public Submitter Id E-MTAB-11437:hPSC1 broker name ArrayExpress cell line hPSC1 cell type neural cell common name human developmental stage embryo disease normal genotype wild type genotype growth condition organoid culture organism part inner cell mass progenitor cell type embryonic stem cell sample name E-MTAB-11437:hPSC1 ERS10540165 SAMEA12941372 9606 Homo sapiens Protocols: Organoids were collected in 1.5ml Eppendorff tubes and washed three times in 1xPBS. The supernatant of the final wash was discarded and organoids were shock frozen on dry ice and stored at -80C. Total RNA was extracted using the QIAGEN RNeasy Plus Micro kit as per manufacturer's instructions. RNA was eluated in 14 μl of RNAse free water. Libraries were prepared from 250ng of each total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760) and the Poly-A mRNA magnetic isolation module (NEB #E7490) according to the provided protocol ENA first public 2022-06-06 ENA last update 2022-06-06 External Id SAMEA12941372 INSDC center alias Centre for Discovery Brain Sciences University of Edinburgh INSDC center name Centre for Discovery Brain Sciences University of Edinburgh INSDC first public 2022-06-06T12:23:35Z INSDC last update 2022-06-06T12:23:35Z INSDC status public Submitter Id E-MTAB-11437:hPSC2 broker name ArrayExpress cell line CS02 cell type neural cell common name human developmental stage embryo disease normal genotype wild type genotype growth condition organoid culture organism part inner cell mass progenitor cell type embryonic stem cell sample name E-MTAB-11437:hPSC2 ERS10540166 SAMEA12941373 9606 Homo sapiens Protocols: Organoids were collected in 1.5ml Eppendorff tubes and washed three times in 1xPBS. The supernatant of the final wash was discarded and organoids were shock frozen on dry ice and stored at -80C. Total RNA was extracted using the QIAGEN RNeasy Plus Micro kit as per manufacturer's instructions. RNA was eluated in 14 μl of RNAse free water. Libraries were prepared from 250ng of each total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760) and the Poly-A mRNA magnetic isolation module (NEB #E7490) according to the provided protocol ENA first public 2022-06-06 ENA last update 2022-06-06 External Id SAMEA12941373 INSDC center alias Centre for Discovery Brain Sciences University of Edinburgh INSDC center name Centre for Discovery Brain Sciences University of Edinburgh INSDC first public 2022-06-06T12:23:35Z INSDC last update 2022-06-06T12:23:35Z INSDC status public Submitter Id E-MTAB-11437:hPSC3 broker name ArrayExpress cell line CS25 cell type neural cell common name human developmental stage embryo disease normal genotype wild type genotype growth condition organoid culture organism part inner cell mass progenitor cell type embryonic stem cell sample name E-MTAB-11437:hPSC3 ERS10540167 SAMEA12941374 9606 Homo sapiens Protocols: Organoids were collected in 1.5ml Eppendorff tubes and washed three times in 1xPBS. The supernatant of the final wash was discarded and organoids were shock frozen on dry ice and stored at -80C. Total RNA was extracted using the QIAGEN RNeasy Plus Micro kit as per manufacturer's instructions. RNA was eluated in 14 μl of RNAse free water. Libraries were prepared from 250ng of each total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760) and the Poly-A mRNA magnetic isolation module (NEB #E7490) according to the provided protocol ENA first public 2022-06-06 ENA last update 2022-06-06 External Id SAMEA12941374 INSDC center alias Centre for Discovery Brain Sciences University of Edinburgh INSDC center name Centre for Discovery Brain Sciences University of Edinburgh INSDC first public 2022-06-06T12:23:35Z INSDC last update 2022-06-06T12:23:35Z INSDC status public Submitter Id E-MTAB-11437:hPSM1 broker name ArrayExpress cell line hPSC1 cell type neural cell common name human developmental stage embryo disease normal genetic modification homozygous INPP5e D477N mutation genotype INPP5eD477N/D477N growth condition organoid culture organism part inner cell mass progenitor cell type embryonic stem cell sample name E-MTAB-11437:hPSM1 ERS10540168 SAMEA12941375 9606 Homo sapiens Protocols: Organoids were collected in 1.5ml Eppendorff tubes and washed three times in 1xPBS. The supernatant of the final wash was discarded and organoids were shock frozen on dry ice and stored at -80C. Total RNA was extracted using the QIAGEN RNeasy Plus Micro kit as per manufacturer's instructions. RNA was eluated in 14 μl of RNAse free water. Libraries were prepared from 250ng of each total-RNA sample using the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760) and the Poly-A mRNA magnetic isolation module (NEB #E7490) according to the provided protocol ENA first public 2022-06-06 ENA last update 2022-06-06 External Id SAMEA12941375 INSDC center alias Centre for Discovery Brain Sciences University of Edinburgh INSDC center name Centre for Discovery Brain Sciences University of Edinburgh INSDC first public 2022-06-06T12:23:35Z INSDC last update 2022-06-06T12:23:35Z INSDC status public Submitter Id E-MTAB-11437:hPSM2 broker name ArrayExpress cell line hPSC2 cell type neural cell common name human developmental stage embryo disease normal genetic modification homozygous INPP5e D477N mutation genotype INPP5eD477N/D477N growth condition organoid culture organism part inner cell mass progenitor cell type embryonic stem cell sample name E-MTAB-11437:hPSM2