ERS10697305 SAMEA13093302 4472 Lemna minor Protocols: For each sample, 25 mg of plant tissue were randomly collected from the test vessel into a tube. After adding the lysis matrix (¼ Ceramic Sphere, MP Biomedicals), the lysis was performed according to the manufacturer's protocol of the RapidPURE RNA Plant Kit (MP Biomedicals Illkirch, France). Homogenization was conducted at 5 m/s for 60 s with FastPrep-24© 5G (MP Biomedicals Illkirch, France) at room temperature. One week before test start, a pre-culture of Lemna minor was auxenically prepared from a stock culture in Steinberg medium according to OECD TG 221. Plants were kept in a growth chamber at 24±2°C and under continuous light (85-135 µE∙m-2∙s-1). Atorvastatin-calcium was purchased from abcr (Karlsruhe, Germany). Test solutions of EC5 and EC20 (% inhibition of growth (e.g. 50 %) is determined and expressed as the ECx e.g. EC50) determined in preliminary experiments according to OECD TG 221, were prepared with Steinberg medium at a pH 5.5±0.2. 150 mL of each test solution were transferred into three test vessels (replicates) and analogously 150 mL of pure Steinberg medium were used as control samples. Four healthy plants with three fronds each were transferred from the pre-culture to the test vessels. Incubation was conducted according to the conditions described in “Growth protocol” under random arrangement for 3 days. Total RNA and protein were extracted according to the RapidPURE Plant Kit (MP Biomedicals Illkirch, France). RNA quantification (RNA>100 ng/µL) was performed using Nanodrop 2000 spectrophotometer (Thermo Scientific), and quality control (RIN>7.0) was conducted using the 2100 Bioanalyzer (Agilent Technologies). The samples were stored at -80°C until mRNA sequencing. cDNA libraries were prepared from mRNA that was purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing. ENA first public 2022-03-31 ENA last update 2022-03-31 External Id SAMEA13093302 INSDC center alias Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC center name Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC first public 2022-03-31T00:18:23Z INSDC last update 2022-03-31T00:18:23Z INSDC status public Submitter Id E-MTAB-11459:R1484 age 7 broker name ArrayExpress developmental stage growth stage extraction date 31.05.2021 genotype wild type genotype organism part whole plant rin 8 sample name E-MTAB-11459:R1484 ERS10697306 SAMEA13093303 4472 Lemna minor Protocols: For each sample, 25 mg of plant tissue were randomly collected from the test vessel into a tube. After adding the lysis matrix (¼ Ceramic Sphere, MP Biomedicals), the lysis was performed according to the manufacturer's protocol of the RapidPURE RNA Plant Kit (MP Biomedicals Illkirch, France). Homogenization was conducted at 5 m/s for 60 s with FastPrep-24© 5G (MP Biomedicals Illkirch, France) at room temperature. One week before test start, a pre-culture of Lemna minor was auxenically prepared from a stock culture in Steinberg medium according to OECD TG 221. Plants were kept in a growth chamber at 24±2°C and under continuous light (85-135 µE∙m-2∙s-1). Atorvastatin-calcium was purchased from abcr (Karlsruhe, Germany). Test solutions of EC5 and EC20 (% inhibition of growth (e.g. 50 %) is determined and expressed as the ECx e.g. EC50) determined in preliminary experiments according to OECD TG 221, were prepared with Steinberg medium at a pH 5.5±0.2. 150 mL of each test solution were transferred into three test vessels (replicates) and analogously 150 mL of pure Steinberg medium were used as control samples. Four healthy plants with three fronds each were transferred from the pre-culture to the test vessels. Incubation was conducted according to the conditions described in “Growth protocol” under random arrangement for 3 days. Total RNA and protein were extracted according to the RapidPURE Plant Kit (MP Biomedicals Illkirch, France). RNA quantification (RNA>100 ng/µL) was performed using Nanodrop 2000 spectrophotometer (Thermo Scientific), and quality control (RIN>7.0) was conducted using the 2100 Bioanalyzer (Agilent Technologies). The samples were stored at -80°C until mRNA sequencing. cDNA libraries were prepared from mRNA that was purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing. ENA first public 2022-03-31 ENA last update 2022-03-31 External Id SAMEA13093303 INSDC center alias Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC center name Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC first public 2022-03-31T00:18:23Z INSDC last update 2022-03-31T00:18:23Z INSDC status public Submitter Id E-MTAB-11459:R1485 age 7 broker name ArrayExpress developmental stage growth stage extraction date 31.05.2021 genotype wild type genotype organism part whole plant rin 8.1 sample name E-MTAB-11459:R1485 ERS10697307 SAMEA13093304 4472 Lemna minor Protocols: For each sample, 25 mg of plant tissue were randomly collected from the test vessel into a tube. After adding the lysis matrix (¼ Ceramic Sphere, MP Biomedicals), the lysis was performed according to the manufacturer's protocol of the RapidPURE RNA Plant Kit (MP Biomedicals Illkirch, France). Homogenization was conducted at 5 m/s for 60 s with FastPrep-24© 5G (MP Biomedicals Illkirch, France) at room temperature. One week before test start, a pre-culture of Lemna minor was auxenically prepared from a stock culture in Steinberg medium according to OECD TG 221. Plants were kept in a growth chamber at 24±2°C and under continuous light (85-135 µE∙m-2∙s-1). Atorvastatin-calcium was purchased from abcr (Karlsruhe, Germany). Test solutions of EC5 and EC20 (% inhibition of growth (e.g. 50 %) is determined and expressed as the ECx e.g. EC50) determined in preliminary experiments according to OECD TG 221, were prepared with Steinberg medium at a pH 5.5±0.2. 150 mL of each test solution were transferred into three test vessels (replicates) and analogously 150 mL of pure Steinberg medium were used as control samples. Four healthy plants with three fronds each were transferred from the pre-culture to the test vessels. Incubation was conducted according to the conditions described in “Growth protocol” under random arrangement for 3 days. Total RNA and protein were extracted according to the RapidPURE Plant Kit (MP Biomedicals Illkirch, France). RNA quantification (RNA>100 ng/µL) was performed using Nanodrop 2000 spectrophotometer (Thermo Scientific), and quality control (RIN>7.0) was conducted using the 2100 Bioanalyzer (Agilent Technologies). The samples were stored at -80°C until mRNA sequencing. cDNA libraries were prepared from mRNA that was purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing. ENA first public 2022-03-31 ENA last update 2022-03-31 External Id SAMEA13093304 INSDC center alias Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC center name Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC first public 2022-03-31T00:18:23Z INSDC last update 2022-03-31T00:18:23Z INSDC status public Submitter Id E-MTAB-11459:R1486 age 7 broker name ArrayExpress developmental stage growth stage extraction date 31.05.2021 genotype wild type genotype organism part whole plant rin 7.8 sample name E-MTAB-11459:R1486 ERS10697308 SAMEA13093305 4472 Lemna minor Protocols: For each sample, 25 mg of plant tissue were randomly collected from the test vessel into a tube. After adding the lysis matrix (¼ Ceramic Sphere, MP Biomedicals), the lysis was performed according to the manufacturer's protocol of the RapidPURE RNA Plant Kit (MP Biomedicals Illkirch, France). Homogenization was conducted at 5 m/s for 60 s with FastPrep-24© 5G (MP Biomedicals Illkirch, France) at room temperature. One week before test start, a pre-culture of Lemna minor was auxenically prepared from a stock culture in Steinberg medium according to OECD TG 221. Plants were kept in a growth chamber at 24±2°C and under continuous light (85-135 µE∙m-2∙s-1). Atorvastatin-calcium was purchased from abcr (Karlsruhe, Germany). Test solutions of EC5 and EC20 (% inhibition of growth (e.g. 50 %) is determined and expressed as the ECx e.g. EC50) determined in preliminary experiments according to OECD TG 221, were prepared with Steinberg medium at a pH 5.5±0.2. 150 mL of each test solution were transferred into three test vessels (replicates) and analogously 150 mL of pure Steinberg medium were used as control samples. Four healthy plants with three fronds each were transferred from the pre-culture to the test vessels. Incubation was conducted according to the conditions described in “Growth protocol” under random arrangement for 3 days. Total RNA and protein were extracted according to the RapidPURE Plant Kit (MP Biomedicals Illkirch, France). RNA quantification (RNA>100 ng/µL) was performed using Nanodrop 2000 spectrophotometer (Thermo Scientific), and quality control (RIN>7.0) was conducted using the 2100 Bioanalyzer (Agilent Technologies). The samples were stored at -80°C until mRNA sequencing. cDNA libraries were prepared from mRNA that was purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing. ENA first public 2022-03-31 ENA last update 2022-03-31 External Id SAMEA13093305 INSDC center alias Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC center name Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC first public 2022-03-31T00:18:23Z INSDC last update 2022-03-31T00:18:23Z INSDC status public Submitter Id E-MTAB-11459:R1487 age 7 broker name ArrayExpress developmental stage growth stage extraction date 31.05.2021 genotype wild type genotype organism part whole plant rin 7.6 sample name E-MTAB-11459:R1487 treatment EC5 ERS10697309 SAMEA13093306 4472 Lemna minor Protocols: For each sample, 25 mg of plant tissue were randomly collected from the test vessel into a tube. After adding the lysis matrix (¼ Ceramic Sphere, MP Biomedicals), the lysis was performed according to the manufacturer's protocol of the RapidPURE RNA Plant Kit (MP Biomedicals Illkirch, France). Homogenization was conducted at 5 m/s for 60 s with FastPrep-24© 5G (MP Biomedicals Illkirch, France) at room temperature. One week before test start, a pre-culture of Lemna minor was auxenically prepared from a stock culture in Steinberg medium according to OECD TG 221. Plants were kept in a growth chamber at 24±2°C and under continuous light (85-135 µE∙m-2∙s-1). Atorvastatin-calcium was purchased from abcr (Karlsruhe, Germany). Test solutions of EC5 and EC20 (% inhibition of growth (e.g. 50 %) is determined and expressed as the ECx e.g. EC50) determined in preliminary experiments according to OECD TG 221, were prepared with Steinberg medium at a pH 5.5±0.2. 150 mL of each test solution were transferred into three test vessels (replicates) and analogously 150 mL of pure Steinberg medium were used as control samples. Four healthy plants with three fronds each were transferred from the pre-culture to the test vessels. Incubation was conducted according to the conditions described in “Growth protocol” under random arrangement for 3 days. Total RNA and protein were extracted according to the RapidPURE Plant Kit (MP Biomedicals Illkirch, France). RNA quantification (RNA>100 ng/µL) was performed using Nanodrop 2000 spectrophotometer (Thermo Scientific), and quality control (RIN>7.0) was conducted using the 2100 Bioanalyzer (Agilent Technologies). The samples were stored at -80°C until mRNA sequencing. cDNA libraries were prepared from mRNA that was purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing. ENA first public 2022-03-31 ENA last update 2022-03-31 External Id SAMEA13093306 INSDC center alias Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC center name Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC first public 2022-03-31T00:18:23Z INSDC last update 2022-03-31T00:18:23Z INSDC status public Submitter Id E-MTAB-11459:R1488 age 7 broker name ArrayExpress developmental stage growth stage extraction date 31.05.2021 genotype wild type genotype organism part whole plant rin 7.7 sample name E-MTAB-11459:R1488 treatment EC5 ERS10697310 SAMEA13093307 4472 Lemna minor Protocols: For each sample, 25 mg of plant tissue were randomly collected from the test vessel into a tube. After adding the lysis matrix (¼ Ceramic Sphere, MP Biomedicals), the lysis was performed according to the manufacturer's protocol of the RapidPURE RNA Plant Kit (MP Biomedicals Illkirch, France). Homogenization was conducted at 5 m/s for 60 s with FastPrep-24© 5G (MP Biomedicals Illkirch, France) at room temperature. One week before test start, a pre-culture of Lemna minor was auxenically prepared from a stock culture in Steinberg medium according to OECD TG 221. Plants were kept in a growth chamber at 24±2°C and under continuous light (85-135 µE∙m-2∙s-1). Atorvastatin-calcium was purchased from abcr (Karlsruhe, Germany). Test solutions of EC5 and EC20 (% inhibition of growth (e.g. 50 %) is determined and expressed as the ECx e.g. EC50) determined in preliminary experiments according to OECD TG 221, were prepared with Steinberg medium at a pH 5.5±0.2. 150 mL of each test solution were transferred into three test vessels (replicates) and analogously 150 mL of pure Steinberg medium were used as control samples. Four healthy plants with three fronds each were transferred from the pre-culture to the test vessels. Incubation was conducted according to the conditions described in “Growth protocol” under random arrangement for 3 days. Total RNA and protein were extracted according to the RapidPURE Plant Kit (MP Biomedicals Illkirch, France). RNA quantification (RNA>100 ng/µL) was performed using Nanodrop 2000 spectrophotometer (Thermo Scientific), and quality control (RIN>7.0) was conducted using the 2100 Bioanalyzer (Agilent Technologies). The samples were stored at -80°C until mRNA sequencing. cDNA libraries were prepared from mRNA that was purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing. ENA first public 2022-03-31 ENA last update 2022-03-31 External Id SAMEA13093307 INSDC center alias Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC center name Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC first public 2022-03-31T00:18:23Z INSDC last update 2022-03-31T00:18:23Z INSDC status public Submitter Id E-MTAB-11459:R1489 age 7 broker name ArrayExpress developmental stage growth stage extraction date 31.05.2021 genotype wild type genotype organism part whole plant rin 8 sample name E-MTAB-11459:R1489 treatment EC5 ERS10697311 SAMEA13093308 4472 Lemna minor Protocols: For each sample, 25 mg of plant tissue were randomly collected from the test vessel into a tube. After adding the lysis matrix (¼ Ceramic Sphere, MP Biomedicals), the lysis was performed according to the manufacturer's protocol of the RapidPURE RNA Plant Kit (MP Biomedicals Illkirch, France). Homogenization was conducted at 5 m/s for 60 s with FastPrep-24© 5G (MP Biomedicals Illkirch, France) at room temperature. One week before test start, a pre-culture of Lemna minor was auxenically prepared from a stock culture in Steinberg medium according to OECD TG 221. Plants were kept in a growth chamber at 24±2°C and under continuous light (85-135 µE∙m-2∙s-1). Atorvastatin-calcium was purchased from abcr (Karlsruhe, Germany). Test solutions of EC5 and EC20 (% inhibition of growth (e.g. 50 %) is determined and expressed as the ECx e.g. EC50) determined in preliminary experiments according to OECD TG 221, were prepared with Steinberg medium at a pH 5.5±0.2. 150 mL of each test solution were transferred into three test vessels (replicates) and analogously 150 mL of pure Steinberg medium were used as control samples. Four healthy plants with three fronds each were transferred from the pre-culture to the test vessels. Incubation was conducted according to the conditions described in “Growth protocol” under random arrangement for 3 days. Total RNA and protein were extracted according to the RapidPURE Plant Kit (MP Biomedicals Illkirch, France). RNA quantification (RNA>100 ng/µL) was performed using Nanodrop 2000 spectrophotometer (Thermo Scientific), and quality control (RIN>7.0) was conducted using the 2100 Bioanalyzer (Agilent Technologies). The samples were stored at -80°C until mRNA sequencing. cDNA libraries were prepared from mRNA that was purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing. ENA first public 2022-03-31 ENA last update 2022-03-31 External Id SAMEA13093308 INSDC center alias Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC center name Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC first public 2022-03-31T00:18:23Z INSDC last update 2022-03-31T00:18:23Z INSDC status public Submitter Id E-MTAB-11459:R1491 age 7 broker name ArrayExpress developmental stage growth stage extraction date 31.05.2021 genotype wild type genotype organism part whole plant rin 7.9 sample name E-MTAB-11459:R1491 treatment EC20 ERS10697312 SAMEA13093309 4472 Lemna minor Protocols: For each sample, 25 mg of plant tissue were randomly collected from the test vessel into a tube. After adding the lysis matrix (¼ Ceramic Sphere, MP Biomedicals), the lysis was performed according to the manufacturer's protocol of the RapidPURE RNA Plant Kit (MP Biomedicals Illkirch, France). Homogenization was conducted at 5 m/s for 60 s with FastPrep-24© 5G (MP Biomedicals Illkirch, France) at room temperature. One week before test start, a pre-culture of Lemna minor was auxenically prepared from a stock culture in Steinberg medium according to OECD TG 221. Plants were kept in a growth chamber at 24±2°C and under continuous light (85-135 µE∙m-2∙s-1). Atorvastatin-calcium was purchased from abcr (Karlsruhe, Germany). Test solutions of EC5 and EC20 (% inhibition of growth (e.g. 50 %) is determined and expressed as the ECx e.g. EC50) determined in preliminary experiments according to OECD TG 221, were prepared with Steinberg medium at a pH 5.5±0.2. 150 mL of each test solution were transferred into three test vessels (replicates) and analogously 150 mL of pure Steinberg medium were used as control samples. Four healthy plants with three fronds each were transferred from the pre-culture to the test vessels. Incubation was conducted according to the conditions described in “Growth protocol” under random arrangement for 3 days. Total RNA and protein were extracted according to the RapidPURE Plant Kit (MP Biomedicals Illkirch, France). RNA quantification (RNA>100 ng/µL) was performed using Nanodrop 2000 spectrophotometer (Thermo Scientific), and quality control (RIN>7.0) was conducted using the 2100 Bioanalyzer (Agilent Technologies). The samples were stored at -80°C until mRNA sequencing. cDNA libraries were prepared from mRNA that was purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing. ENA first public 2022-03-31 ENA last update 2022-03-31 External Id SAMEA13093309 INSDC center alias Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC center name Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC first public 2022-03-31T00:18:23Z INSDC last update 2022-03-31T00:18:23Z INSDC status public Submitter Id E-MTAB-11459:R1493 age 7 broker name ArrayExpress developmental stage growth stage extraction date 31.05.2021 genotype wild type genotype organism part whole plant rin 7.9 sample name E-MTAB-11459:R1493 treatment EC20 ERS10697313 SAMEA13093310 4472 Lemna minor Protocols: For each sample, 25 mg of plant tissue were randomly collected from the test vessel into a tube. After adding the lysis matrix (¼ Ceramic Sphere, MP Biomedicals), the lysis was performed according to the manufacturer's protocol of the RapidPURE RNA Plant Kit (MP Biomedicals Illkirch, France). Homogenization was conducted at 5 m/s for 60 s with FastPrep-24© 5G (MP Biomedicals Illkirch, France) at room temperature. One week before test start, a pre-culture of Lemna minor was auxenically prepared from a stock culture in Steinberg medium according to OECD TG 221. Plants were kept in a growth chamber at 24±2°C and under continuous light (85-135 µE∙m-2∙s-1). Atorvastatin-calcium was purchased from abcr (Karlsruhe, Germany). Test solutions of EC5 and EC20 (% inhibition of growth (e.g. 50 %) is determined and expressed as the ECx e.g. EC50) determined in preliminary experiments according to OECD TG 221, were prepared with Steinberg medium at a pH 5.5±0.2. 150 mL of each test solution were transferred into three test vessels (replicates) and analogously 150 mL of pure Steinberg medium were used as control samples. Four healthy plants with three fronds each were transferred from the pre-culture to the test vessels. Incubation was conducted according to the conditions described in “Growth protocol” under random arrangement for 3 days. Total RNA and protein were extracted according to the RapidPURE Plant Kit (MP Biomedicals Illkirch, France). RNA quantification (RNA>100 ng/µL) was performed using Nanodrop 2000 spectrophotometer (Thermo Scientific), and quality control (RIN>7.0) was conducted using the 2100 Bioanalyzer (Agilent Technologies). The samples were stored at -80°C until mRNA sequencing. cDNA libraries were prepared from mRNA that was purified through PolyA selection using the TruSeq RNA Library Prep Kit v2 (Illumina). Libraries were validated using a Fragment Analyzer system (Agilent, Santa Clara, USA) before sequencing. ENA first public 2022-03-31 ENA last update 2022-03-31 External Id SAMEA13093310 INSDC center alias Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC center name Fraunhofer Institute for Molecular Biology and Applied Ecology INSDC first public 2022-03-31T00:18:23Z INSDC last update 2022-03-31T00:18:23Z INSDC status public Submitter Id E-MTAB-11459:R1494 age 7 broker name ArrayExpress developmental stage growth stage extraction date 31.05.2021 genotype wild type genotype organism part whole plant rin 7.9 sample name E-MTAB-11459:R1494 treatment EC20