ERS1847866 SAMEA104188848 10116 Rattus norvegicus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188848 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:BR1_IFNB broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Norway rat disease normal individual BR1 organism part dermis sample name E-MTAB-5919:BR1_IFNB sex female strain BN ERS1847867 SAMEA104188849 10116 Rattus norvegicus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188849 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:BR1_LF4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Norway rat disease normal individual BR1 organism part dermis sample name E-MTAB-5919:BR1_LF4 sex female strain BN ERS1847868 SAMEA104188850 10116 Rattus norvegicus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188850 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:BR1_PIC4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Norway rat disease normal individual BR1 organism part dermis sample name E-MTAB-5919:BR1_PIC4 sex female strain BN ERS1847869 SAMEA104188851 10116 Rattus norvegicus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188851 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:BR1_UNST broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Norway rat disease normal individual BR1 organism part dermis sample name E-MTAB-5919:BR1_UNST sex female strain BN ERS1847870 SAMEA104188852 10116 Rattus norvegicus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188852 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:BR2_IFNB broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Norway rat disease normal individual BR2 organism part dermis sample name E-MTAB-5919:BR2_IFNB sex female strain BN ERS1847871 SAMEA104188853 10116 Rattus norvegicus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188853 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:BR2_LF4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Norway rat disease normal individual BR2 organism part dermis sample name E-MTAB-5919:BR2_LF4 sex female strain BN ERS1847872 SAMEA104188854 10116 Rattus norvegicus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188854 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:BR2_PIC4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Norway rat disease normal individual BR2 organism part dermis sample name E-MTAB-5919:BR2_PIC4 sex female strain BN ERS1847873 SAMEA104188855 10116 Rattus norvegicus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188855 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:BR2_UNST broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Norway rat disease normal individual BR2 organism part dermis sample name E-MTAB-5919:BR2_UNST sex female strain BN ERS1847874 SAMEA104188856 10116 Rattus norvegicus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188856 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:BR3_IFNB broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Norway rat disease normal individual BR3 organism part dermis sample name E-MTAB-5919:BR3_IFNB sex female strain BN ERS1847875 SAMEA104188857 10116 Rattus norvegicus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188857 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:BR3_LF4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Norway rat disease normal individual BR3 organism part dermis sample name E-MTAB-5919:BR3_LF4 sex female strain BN ERS1847876 SAMEA104188858 10116 Rattus norvegicus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188858 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:BR3_PIC4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Norway rat disease normal individual BR3 organism part dermis sample name E-MTAB-5919:BR3_PIC4 sex female strain BN ERS1847877 SAMEA104188859 10116 Rattus norvegicus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188859 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:BR3_UNST broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Norway rat disease normal individual BR3 organism part dermis sample name E-MTAB-5919:BR3_UNST sex female strain BN ERS1847878 SAMEA104188860 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188860 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS1_CMI broker name ArrayExpress cell line HPSI1013pf-cups cell type fibroblast common name human disease normal individual cups organism part dermis sample name E-MTAB-5919:HS1_CMI sex female ERS1847879 SAMEA104188861 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188861 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS1_IFNB broker name ArrayExpress cell line HPSI1013pf-cups cell type fibroblast common name human disease normal individual cups organism part dermis sample name E-MTAB-5919:HS1_IFNB sex female ERS1847880 SAMEA104188862 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188862 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS1_LF4 broker name ArrayExpress cell line HPSI1013pf-cups cell type fibroblast common name human disease normal individual cups organism part dermis sample name E-MTAB-5919:HS1_LF4 sex female ERS1847881 SAMEA104188863 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188863 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS1_PIC4 broker name ArrayExpress cell line HPSI1013pf-cups cell type fibroblast common name human disease normal individual cups organism part dermis sample name E-MTAB-5919:HS1_PIC4 sex female ERS1847882 SAMEA104188864 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188864 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS1_UNST broker name ArrayExpress cell line HPSI1013pf-cups cell type fibroblast common name human disease normal individual cups organism part dermis sample name E-MTAB-5919:HS1_UNST sex female ERS1847883 SAMEA104188865 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188865 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS2_CMI broker name ArrayExpress cell line HPSI0913pf-oapg cell type fibroblast common name human disease normal individual oapg organism part dermis sample name E-MTAB-5919:HS2_CMI sex female ERS1847884 SAMEA104188866 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188866 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS2_IFNB broker name ArrayExpress cell line HPSI0913pf-oapg cell type fibroblast common name human disease normal individual oapg organism part dermis sample name E-MTAB-5919:HS2_IFNB sex female ERS1847885 SAMEA104188867 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188867 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS2_LF4 broker name ArrayExpress cell line HPSI0913pf-oapg cell type fibroblast common name human disease normal individual oapg organism part dermis sample name E-MTAB-5919:HS2_LF4 sex female ERS1847886 SAMEA104188868 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188868 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS2_PIC4 broker name ArrayExpress cell line HPSI0913pf-oapg cell type fibroblast common name human disease normal individual oapg organism part dermis sample name E-MTAB-5919:HS2_PIC4 sex female ERS1847887 SAMEA104188869 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188869 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS2_UNST broker name ArrayExpress cell line HPSI0913pf-oapg cell type fibroblast common name human disease normal individual oapg organism part dermis sample name E-MTAB-5919:HS2_UNST sex female ERS1847888 SAMEA104188870 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188870 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS3_CMI broker name ArrayExpress cell line HPSI0114pf-joxm cell type fibroblast common name human disease normal individual joxm organism part dermis sample name E-MTAB-5919:HS3_CMI sex female ERS1847889 SAMEA104188871 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188871 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS3_IFNB broker name ArrayExpress cell line HPSI0114pf-joxm cell type fibroblast common name human disease normal individual joxm organism part dermis sample name E-MTAB-5919:HS3_IFNB sex female ERS1847890 SAMEA104188872 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188872 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS3_LF4 broker name ArrayExpress cell line HPSI0114pf-joxm cell type fibroblast common name human disease normal individual joxm organism part dermis sample name E-MTAB-5919:HS3_LF4 sex female ERS1847891 SAMEA104188873 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188873 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS3_PIC4 broker name ArrayExpress cell line HPSI0114pf-joxm cell type fibroblast common name human disease normal individual joxm organism part dermis sample name E-MTAB-5919:HS3_PIC4 sex female ERS1847892 SAMEA104188874 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188874 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS3_UNST broker name ArrayExpress cell line HPSI0114pf-joxm cell type fibroblast common name human disease normal individual joxm organism part dermis sample name E-MTAB-5919:HS3_UNST sex female ERS1847893 SAMEA104188875 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188875 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS4_CMI broker name ArrayExpress cell line HPSI0314pf-sojd cell type fibroblast common name human disease normal individual sojd organism part dermis sample name E-MTAB-5919:HS4_CMI sex female ERS1847894 SAMEA104188876 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188876 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS4_IFNB broker name ArrayExpress cell line HPSI0314pf-sojd cell type fibroblast common name human disease normal individual sojd organism part dermis sample name E-MTAB-5919:HS4_IFNB sex female ERS1847895 SAMEA104188877 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188877 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:35Z INSDC status public Submitter Id E-MTAB-5919:HS4_LF4 broker name ArrayExpress cell line HPSI0314pf-sojd cell type fibroblast common name human disease normal individual sojd organism part dermis sample name E-MTAB-5919:HS4_LF4 sex female ERS1847896 SAMEA104188878 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188878 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:HS4_PIC4 broker name ArrayExpress cell line HPSI0314pf-sojd cell type fibroblast common name human disease normal individual sojd organism part dermis sample name E-MTAB-5919:HS4_PIC4 sex female ERS1847897 SAMEA104188879 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188879 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:HS4_UNST broker name ArrayExpress cell line HPSI0314pf-sojd cell type fibroblast common name human disease normal individual sojd organism part dermis sample name E-MTAB-5919:HS4_UNST sex female ERS1847898 SAMEA104188880 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188880 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:HS5_CMI broker name ArrayExpress cell line HPSI0214pf-pelm cell type fibroblast common name human disease normal individual pelm organism part dermis sample name E-MTAB-5919:HS5_CMI sex female ERS1847899 SAMEA104188881 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188881 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:HS5_IFNB broker name ArrayExpress cell line HPSI0214pf-pelm cell type fibroblast common name human disease normal individual pelm organism part dermis sample name E-MTAB-5919:HS5_IFNB sex female ERS1847900 SAMEA104188882 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188882 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:HS5_LF4 broker name ArrayExpress cell line HPSI0214pf-pelm cell type fibroblast common name human disease normal individual pelm organism part dermis sample name E-MTAB-5919:HS5_LF4 sex female ERS1847901 SAMEA104188883 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188883 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:HS5_PIC4 broker name ArrayExpress cell line HPSI0214pf-pelm cell type fibroblast common name human disease normal individual pelm organism part dermis sample name E-MTAB-5919:HS5_PIC4 sex female ERS1847902 SAMEA104188884 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188884 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:HS5_UNST broker name ArrayExpress cell line HPSI0214pf-pelm cell type fibroblast common name human disease normal individual pelm organism part dermis sample name E-MTAB-5919:HS5_UNST sex female ERS1847903 SAMEA104188885 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188885 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:HS6_CMI broker name ArrayExpress cell line HPSI0314pf-hoik cell type fibroblast common name human disease normal individual hoik organism part dermis sample name E-MTAB-5919:HS6_CMI sex female ERS1847904 SAMEA104188886 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188886 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:HS6_IFNB broker name ArrayExpress cell line HPSI0314pf-hoik cell type fibroblast common name human disease normal individual hoik organism part dermis sample name E-MTAB-5919:HS6_IFNB sex female ERS1847905 SAMEA104188887 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188887 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:HS6_LF4 broker name ArrayExpress cell line HPSI0314pf-hoik cell type fibroblast common name human disease normal individual hoik organism part dermis sample name E-MTAB-5919:HS6_LF4 sex female ERS1847906 SAMEA104188888 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188888 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:HS6_PIC4 broker name ArrayExpress cell line HPSI0314pf-hoik cell type fibroblast common name human disease normal individual hoik organism part dermis sample name E-MTAB-5919:HS6_PIC4 sex female ERS1847907 SAMEA104188889 9606 Homo sapiens Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188889 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:HS6_UNST broker name ArrayExpress cell line HPSI0314pf-hoik cell type fibroblast common name human disease normal individual hoik organism part dermis sample name E-MTAB-5919:HS6_UNST sex female ERS1847908 SAMEA104188890 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188890 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM1_CMI broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM1 organism part dermis sample name E-MTAB-5919:MM1_CMI sex female strain C57BL/6 ERS1847909 SAMEA104188891 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188891 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM1_IFNB broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM1 organism part dermis sample name E-MTAB-5919:MM1_IFNB sex female strain C57BL/6 ERS1847910 SAMEA104188892 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188892 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM1_LF4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM1 organism part dermis sample name E-MTAB-5919:MM1_LF4 sex female strain C57BL/6 ERS1847911 SAMEA104188893 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188893 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM1_PIC4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM1 organism part dermis sample name E-MTAB-5919:MM1_PIC4 sex female strain C57BL/6 ERS1847912 SAMEA104188894 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188894 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM1_UNST broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM1 organism part dermis sample name E-MTAB-5919:MM1_UNST sex female strain C57BL/6 ERS1847913 SAMEA104188895 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188895 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM2_CMI broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM2 organism part dermis sample name E-MTAB-5919:MM2_CMI sex female strain C57BL/6 ERS1847914 SAMEA104188896 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188896 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM2_IFNB broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM2 organism part dermis sample name E-MTAB-5919:MM2_IFNB sex female strain C57BL/6 ERS1847915 SAMEA104188897 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188897 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM2_LF4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM2 organism part dermis sample name E-MTAB-5919:MM2_LF4 sex female strain C57BL/6 ERS1847916 SAMEA104188898 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188898 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM2_PIC4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM2 organism part dermis sample name E-MTAB-5919:MM2_PIC4 sex female strain C57BL/6 ERS1847917 SAMEA104188899 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188899 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM2_UNST broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM2 organism part dermis sample name E-MTAB-5919:MM2_UNST sex female strain C57BL/6 ERS1847918 SAMEA104188900 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188900 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM3_CMI broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM3 organism part dermis sample name E-MTAB-5919:MM3_CMI sex female strain C57BL/6 ERS1847919 SAMEA104188901 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188901 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM3_IFNB broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM3 organism part dermis sample name E-MTAB-5919:MM3_IFNB sex female strain C57BL/6 ERS1847920 SAMEA104188902 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188902 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM3_LF4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM3 organism part dermis sample name E-MTAB-5919:MM3_LF4 sex female strain C57BL/6 ERS1847921 SAMEA104188903 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188903 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM3_PIC4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM3 organism part dermis sample name E-MTAB-5919:MM3_PIC4 sex female strain C57BL/6 ERS1847922 SAMEA104188904 10090 Mus musculus Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188904 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:MM3_UNST broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name house mouse disease normal individual MM3 organism part dermis sample name E-MTAB-5919:MM3_UNST sex female strain C57BL/6 ERS1847923 SAMEA104188905 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188905 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH1_IFNB broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH1 organism part dermis sample name E-MTAB-5919:RH1_IFNB sex female ERS1847924 SAMEA104188906 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188906 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH1_UNST broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH1 organism part dermis sample name E-MTAB-5919:RH1_UNST sex female ERS1847925 SAMEA104188907 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188907 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH2_IFNB broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH2 organism part dermis sample name E-MTAB-5919:RH2_IFNB sex female ERS1847926 SAMEA104188908 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188908 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH2_LF4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH2 organism part dermis sample name E-MTAB-5919:RH2_LF4 sex female ERS1847927 SAMEA104188909 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188909 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH2_PIC4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH2 organism part dermis sample name E-MTAB-5919:RH2_PIC4 sex female ERS1847928 SAMEA104188910 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188910 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH2_UNST broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH2 organism part dermis sample name E-MTAB-5919:RH2_UNST sex female ERS1847929 SAMEA104188911 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188911 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH3_IFNB broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH3 organism part dermis sample name E-MTAB-5919:RH3_IFNB sex female ERS1847930 SAMEA104188912 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188912 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH3_LF4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH3 organism part dermis sample name E-MTAB-5919:RH3_LF4 sex female ERS1847931 SAMEA104188913 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188913 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH3_PIC4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH3 organism part dermis sample name E-MTAB-5919:RH3_PIC4 sex female ERS1847932 SAMEA104188914 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188914 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH3_UNST broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH3 organism part dermis sample name E-MTAB-5919:RH3_UNST sex female ERS1847933 SAMEA104188915 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188915 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH4_IFNB broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH4 organism part dermis sample name E-MTAB-5919:RH4_IFNB sex female ERS1847934 SAMEA104188916 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188916 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH4_LF4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH4 organism part dermis sample name E-MTAB-5919:RH4_LF4 sex female ERS1847935 SAMEA104188917 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188917 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH4_PIC4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH4 organism part dermis sample name E-MTAB-5919:RH4_PIC4 sex female ERS1847936 SAMEA104188918 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188918 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH4_UNST broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH4 organism part dermis sample name E-MTAB-5919:RH4_UNST sex female ERS1847937 SAMEA104188919 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188919 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH5_IFNB broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH5 organism part dermis sample name E-MTAB-5919:RH5_IFNB sex female ERS1847938 SAMEA104188920 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188920 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH5_LF4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH5 organism part dermis sample name E-MTAB-5919:RH5_LF4 sex female ERS1847939 SAMEA104188921 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188921 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH5_PIC4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH5 organism part dermis sample name E-MTAB-5919:RH5_PIC4 sex female ERS1847940 SAMEA104188922 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188922 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH5_UNST broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH5 organism part dermis sample name E-MTAB-5919:RH5_UNST sex female ERS1847941 SAMEA104188923 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188923 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH6_IFNB broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH6 organism part dermis sample name E-MTAB-5919:RH6_IFNB sex female ERS1847942 SAMEA104188924 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188924 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH6_LF4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH6 organism part dermis sample name E-MTAB-5919:RH6_LF4 sex female ERS1847943 SAMEA104188925 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188925 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:36Z INSDC status public Submitter Id E-MTAB-5919:RH6_PIC4 broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH6 organism part dermis sample name E-MTAB-5919:RH6_PIC4 sex female ERS1847944 SAMEA104188926 9544 Macaca mulatta Protocols: Cells were washed with PBS and then lysed in RLT plus buffer (Qiagen) Human dermal fibroblasts were obtained from the HipSci consortium (http://www.hipsci.org/ (Streeter et al., 2017) (where they were used for creating iPSCs). These original cells were thawed to test for growth and viability and refrozen after 1-2 passages, so that all lines were in the same growth rate and proliferation conditions before the stimulation experiment. Non-human primate cells were extracted from skin tissues that were incubated for 2hrs with 0.5% collagense-A (Roche; 11088815001) after mechanical processing, and then filtered through 100µm strainers before being plated and passaged prior to cryo-banking. Rodent cells were obtained from Cellbiologics where they were extracted using a similar enzymatic extraction protocol using skin tissues from the same regions of the body used in primates. Cell were grown in ATCC fibroblast growth medium (Fibroblast Basal Medium (ATCC, ATCC-PCS-201-030) with Fibroblast Growth Kit-Low serum (ATCC, PCS-201-041) Cells were stimulated with either: (1) 1ug/mL High-Molecular Weight poly I:C (Invivogen, Cat. Code: tlrl-pic) transfected with 2uL/mL Lipofectamin 2,000 (ThermoFisher, Cat Number 11668027); (2) mock transfected with Lipofectamin 2,000; (3) stimulated with 1,000 IU of IFNB (human IFNB - 11410-2 (for human and macaque cells); rat IFNB - 13400-1; mouse IFNB - 12401-1; all IFNs were obtained from PBL, and had activity units based on similar virological assays); or (4) left untreated. Additional samples in human and mouse were stimulated with 1,000 IU of Cross-mammalian IFN (Universal Type I IFN Alpha, PBL, Cat Number 11200-1). Total RNA was extracted using the RNeasy Plus Mini kit(Qiagen, Cat Number 74136), using QIAcube (Qiagen). Libraries were produced using the Kapa Stranded mRNA-seq Kit (Kapa Biosystems, Kit code: KK8421). The Kapa library construction protocol was modified for automated library preparation by Bravo (Agilent Technologies). cDNA was amplified in 13 PCR cycles, and purified by Ampure XP beads (Beckman Coulter, Cat Number A63882) (1.8x volume) using Zephyr (Perkin Elmer). Prior to sequencing, libraries were quantified using MiSeq (25bp PE). This data was used for pooling libraries in equimolar amounts by Coulter NX (Span-8, Beckman). ENA first public 2018-05-08 ENA last update 2017-08-01 External Id SAMEA104188926 INSDC center alias EMBL - EBI & Wellcome Trust Sanger Institute INSDC center name EMBL - EBI & Wellcome Trust Sanger Institute INSDC first public 2018-05-08T17:02:09Z INSDC last update 2017-08-01T15:20:37Z INSDC status public Submitter Id E-MTAB-5919:RH6_UNST broker name ArrayExpress cell line fibroblast derived cell line cell type fibroblast common name Rhesus monkey disease normal individual RH6 organism part dermis sample name E-MTAB-5919:RH6_UNST sex female