<?xml version="1.0" encoding="UTF-8"?>
<SAMPLE_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <SAMPLE center_name="Center for Research" alias="35 BACs pool" accession="SRS117315">
    <IDENTIFIERS>
      <PRIMARY_ID>SRS117315</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioSample">SAMN00115782</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>Pool of 35 BACs from the C. melo PIT92 line</TITLE>
    <SAMPLE_NAME>
      <TAXON_ID>412675</TAXON_ID>
      <SCIENTIFIC_NAME>Cucumis melo subsp. melo</SCIENTIFIC_NAME>
    </SAMPLE_NAME>
    <DESCRIPTION>Pool of 35 BACs from a BamHI BAC library of young leaves from the double-haploid melon line 'PIT92' (PI 161375 x T111) Cucumis melo. Library is described in van Leeuwen et al., Plant Mol Biol 2003, 51: 703-718. The pool contains the previously sanger sequenced BAC Cm13_J04 (GenBank Acc. No. EF657230.1). Sequencing was performed with a Roche 454 Genome Sequencer machine using FLX chemistry. Two DNA extractions were done from the 35-BACs pool, one to create a shotgun library and the other one to create a 3 kb paired-end library. The shotgun library was used for one titration run and one full run. The paired-end library was sequenced on two quarters of a plate followed by a full run.</DESCRIPTION>
  </SAMPLE>
  <SAMPLE center_name="Center for Research" alias="23 BACs pool" accession="SRS117316">
    <IDENTIFIERS>
      <PRIMARY_ID>SRS117316</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioSample">SAMN00115783</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>Pool of 35 BACs from the C. melo PIT92 line</TITLE>
    <SAMPLE_NAME>
      <TAXON_ID>412675</TAXON_ID>
      <SCIENTIFIC_NAME>Cucumis melo subsp. melo</SCIENTIFIC_NAME>
    </SAMPLE_NAME>
    <DESCRIPTION>Pool of 23 BACs from a BamHI BAC library of young leaves from the double-haploid melon line 'PIT92' (PI 161375 x T111) Cucumis melo. Library is described in van Leeuwen et al., Plant Mol Biol 2003, 51: 703-718. The chosen BACs map to at least 21 different genetic loci. Sequencing was performed with a Roche 454 Genome Sequencer machine using FLX chemistry. One DNA extraction was done which served to create a shotgun and a 3 kb paired-end library. The shotgun library was sequenced with a full run while the paired-end library was sequenced on three eights of a plate.</DESCRIPTION>
  </SAMPLE>
</SAMPLE_SET>
