SRX028781
Santa Cruz sequencing
Santa Cruz sequenced in half of a picotiter plate
SRP003815
T. californicus transcriptome 454 pyrosequencing
SRS117350
Santa Cruz T. californicus
Santa Cruz cDNA
EST
TRANSCRIPTOMIC
cDNA
0
Technical Read
Adapter
1
1
Application Read
Forward
5
454 GS FLX
SRX028782
San Diego control - sequecing
San Diego unstressed sample - 1/6 of a picotiter plate
SRP003815
T. californicus transcriptome 454 pyrosequencing
Total RNA was extracted from the sample pool of individuals (> 0.5 g wet weight each) using Tri Reagent (Sigma-Aldrich) and mRNA was extracted from the total RNA using Poly(A) Purist Kit (Ambion). Complementary DNA (cDNA) library were prepared using Roche’s GS FLX Titanium Rapid Library Preparation Kit, and San Diego samples barcoded with multiplex identifier (MID) adaptors. Sequencing was performed by Roche Life Sciences.
SRS117351
San Diego untreated sample
San Diego - control
EST
TRANSCRIPTOMIC
cDNA
0
Technical Read
Adapter
1
1
Application Read
Forward
5
454 GS FLX
SRX028783
San Diego - 30 min
San Diego subjected to hyperosmotic stress for 30 minutes
SRP003815
T. californicus transcriptome 454 pyrosequencing
Total RNA was extracted from the sample pool of individuals (> 0.5 g wet weight each) using Tri Reagent (Sigma-Aldrich) and mRNA was extracted from the total RNA using Poly(A) Purist Kit (Ambion). Complementary DNA (cDNA) library were prepared using Roche’s GS FLX Titanium Rapid Library Preparation Kit, and San Diego samples barcoded with multiplex identifier (MID) adaptors. Sequencing was performed by Roche Life Sciences.
SRS117352
San Diego hyperosmotic - 30 min
San Diego - 30 min
EST
TRANSCRIPTOMIC
cDNA
0
Technical Read
Adapter
1
1
Application Read
Forward
5
454 GS FLX
SRX028784
San Diego - 24 hr
San Diego copepods subjected to hyperosmotic stress for 24 hr
SRP003815
T. californicus transcriptome 454 pyrosequencing
Total RNA was extracted from the sample pool of individuals (> 0.5 g wet weight each) using Tri Reagent (Sigma-Aldrich) and mRNA was extracted from the total RNA using Poly(A) Purist Kit (Ambion). Complementary DNA (cDNA) library were prepared using Roche’s GS FLX Titanium Rapid Library Preparation Kit, and San Diego samples barcoded with multiplex identifier (MID) adaptors. Sequencing was performed by Roche Life Sciences.
SRS117353
San Diego hyperosmotic - 24hr
San Diego - 24 hr
EST
TRANSCRIPTOMIC
cDNA
0
Technical Read
Adapter
1
1
Application Read
Forward
5
454 GS FLX