SRX070126 FGMG SRP006965 FGMG cDNA from the Clontech SMART PCR cDNA Synthesis Kit was used as the basis for the library preparation. Following the illumina Paired-End Sample Prep kit protocol (recounted briefly here), cDNA was first sheared using a nebulizer and then purified. DNA fragment ends were repaired, dA was added to the ends, and adapters for the illumina sequencing protocol were added. The prepared DNA fragments were size-selected via agarose gel electrophoresis at a median of ~350 bp, and purified via the Wizard SV Gel and PCR Cleanup System (Promega). Library Construction Date: 4/2/10 SRS211546 FGMG-S1 MaizeSeedling1 RNA-Seq TRANSCRIPTOMIC size fractionation 160 0 Application Read Forward 1 1 Application Read Reverse 81 Illumina Genome Analyzer IIx SRX072631 P1 Sequence SRP006965 FGMG cDNA from the Clontech SMART PCR cDNA Synthesis Kit was used as the basis for the library preparation. Following the illumina Paired-End Sample Prep kit protocol (recounted briefly here), cDNA was first sheared using a nebulizer and then purified. DNA fragment ends were repaired, dA was added to the ends, and adapters for the illumina sequencing protocol were added. The prepared DNA fragments were size-selected via agarose gel electrophoresis at a median of ~320 bp, and purified via the Wizard SV Gel and PCR Cleanup System (Promega). Library Construction Date: 12/9/08 SRS211657 FGMG-P1 MaizePollen1 RNA-Seq TRANSCRIPTOMIC size fractionation 160 0 Application Read Forward 1 1 Application Read Reverse 81 Illumina Genome Analyzer IIx SRX072632 Seedling2 Sequence SRP006965 FGMG cDNA from the Clontech SMART PCR cDNA Synthesis Kit was used as the basis for the library preparation. Following the illumina Paired-End Sample Prep kit protocol (recounted briefly here), cDNA was first sheared using a nebulizer and then purified. DNA fragment ends were repaired, dA was added to the ends, and adapters for the illumina sequencing protocol were added. The prepared DNA fragments were size-selected via agarose gel electrophoresis at a median of ~320 bp, and purified via the Wizard SV Gel and PCR Cleanup System (Promega). Library Construction Date: 12/9/08 SRS211655 FGMG-S2 MaizeSeedling2 RNA-Seq TRANSCRIPTOMIC size fractionation 160 0 Application Read Forward 1 1 Application Read Reverse 81 Illumina Genome Analyzer IIx SRX072635 Seedling3 Sequence SRP006965 FGMG cDNA from the Clontech SMART PCR cDNA Synthesis Kit was used as the basis for the library preparation. Following the illumina Paired-End Sample Prep kit protocol (recounted briefly here), cDNA was first sheared using a nebulizer and then purified. DNA fragment ends were repaired, dA was added to the ends, and adapters for the illumina sequencing protocol were added. The prepared DNA fragments were size-selected via agarose gel electrophoresis at a median of ~320 bp, and purified via the Wizard SV Gel and PCR Cleanup System (Promega). Library Construction Date: 12/9/08 SRS211656 FGMG-S3 MaizeSeedling3 RNA-Seq TRANSCRIPTOMIC size fractionation 160 0 Application Read Forward 1 1 Application Read Reverse 81 Illumina Genome Analyzer IIx SRX072636 Pollen2 Sequence SRP006965 FGMG cDNA from the Clontech SMART PCR cDNA Synthesis Kit was used as the basis for the library preparation. Following the illumina Paired-End Sample Prep kit protocol (recounted briefly here), cDNA was first sheared using a nebulizer and then purified. DNA fragment ends were repaired, dA was added to the ends, and adapters for the illumina sequencing protocol were added. The prepared DNA fragments were size-selected via agarose gel electrophoresis at a median of ~320 bp, and purified via the Wizard SV Gel and PCR Cleanup System (Promega). Library Construction Date: 12/9/08 SRS211658 FGMG-P2 MaizePollen2 RNA-Seq TRANSCRIPTOMIC size fractionation 160 0 Application Read Forward 1 1 Application Read Reverse 81 Illumina Genome Analyzer IIx SRX072637 Pollen3 Sequence SRP006965 FGMG cDNA from the Clontech SMART PCR cDNA Synthesis Kit was used as the basis for the library preparation. Following the illumina Paired-End Sample Prep kit protocol (recounted briefly here), cDNA was first sheared using a nebulizer and then purified. DNA fragment ends were repaired, dA was added to the ends, and adapters for the illumina sequencing protocol were added. The prepared DNA fragments were size-selected via agarose gel electrophoresis at a median of ~320 bp, and purified via the Wizard SV Gel and PCR Cleanup System (Promega). Library Construction Date: 12/9/08 SRS211659 FGMG-P3 MaizePollen3 RNA-Seq TRANSCRIPTOMIC size fractionation 160 0 Application Read Forward 1 1 Application Read Reverse 81 Illumina Genome Analyzer IIx SRX072638 Ovule1 Sequence SRP006965 FGMG cDNA from the Clontech SMARTer PCR cDNA Synthesis Kit was used as the basis for the library preparation. (NOTE that the SMARTer protocol is optimized for small tissue samples, and slightly modified from the SMART protocol used for the Seedling and Pollen samples.) Following the illumina Paired-End Sample Prep kit protocol (recounted briefly here), cDNA was first sheared using a nebulizer and then purified. DNA fragment ends were repaired, dA was added to the ends, and adapters for the illumina sequencing protocol were added. The prepared DNA fragments were size-selected via agarose gel electrophoresis at a median of ~320 bp, and purified via the Wizard SV Gel and PCR Cleanup System (Promega). Library Construction Date: 9/30/09 SRS211660 FGMG-O1 MaizeOvule1 RNA-Seq TRANSCRIPTOMIC size fractionation 160 0 Application Read Forward 1 1 Application Read Reverse 81 Illumina Genome Analyzer IIx SRX072639 Ovule2 Sequence SRP006965 FGMG cDNA from the Clontech SMARTer PCR cDNA Synthesis Kit was used as the basis for the library preparation. (NOTE that the SMARTer protocol is optimized for small tissue samples, and slightly modified from the SMART protocol used for the Seedling and Pollen samples.) Following the illumina Paired-End Sample Prep kit protocol (recounted briefly here), cDNA was first sheared using a nebulizer and then purified. DNA fragment ends were repaired, dA was added to the ends, and adapters for the illumina sequencing protocol were added. The prepared DNA fragments were size-selected via agarose gel electrophoresis at a median of ~320 bp, and purified via the Wizard SV Gel and PCR Cleanup System (Promega). Library Construction Date: 9/30/09 SRS211661 FGMG-O2 MaizeOvule2 RNA-Seq TRANSCRIPTOMIC size fractionation 160 0 Application Read Forward 1 1 Application Read Reverse 81 Illumina Genome Analyzer IIx SRX072640 Ovule3 Sequence SRP006965 FGMG cDNA from the Clontech SMARTer PCR cDNA Synthesis Kit was used as the basis for the library preparation. (NOTE that the SMARTer protocol is optimized for small tissue samples, and slightly modified from the SMART protocol used for the Seedling and Pollen samples.) Following the illumina Paired-End Sample Prep kit protocol (recounted briefly here), cDNA was first sheared using a nebulizer and then purified. DNA fragment ends were repaired, dA was added to the ends, and adapters for the illumina sequencing protocol were added. The prepared DNA fragments were size-selected via agarose gel electrophoresis at a median of ~320 bp, and purified via the Wizard SV Gel and PCR Cleanup System (Promega). Library Construction Date: 9/30/09 SRS211662 FGMG-O3 MaizeOvule3 RNA-Seq TRANSCRIPTOMIC size fractionation 160 0 Application Read Forward 1 1 Application Read Reverse 81 Illumina Genome Analyzer IIx SRX072646 Esac1 Sequence SRP006965 FGMG cDNA from the Clontech SMARTer PCR cDNA Synthesis Kit was used as the basis for the library preparation. (NOTE that the SMARTer protocol is optimized for small tissue samples, and slightly modified from the SMART protocol used for the Seedling and Pollen samples.) Following the illumina Paired-End Sample Prep kit protocol (recounted briefly here), cDNA was first sheared using a nebulizer and then purified. DNA fragment ends were repaired, dA was added to the ends, and adapters for the illumina sequencing protocol were added. The prepared DNA fragments were size-selected via agarose gel electrophoresis at a median of ~320 bp, and purified via the Wizard SV Gel and PCR Cleanup System (Promega). Library Construction Date: 9/30/09 SRS211663 FGMG-ES1 MaizeEmbryoSac1 RNA-Seq TRANSCRIPTOMIC size fractionation 160 0 Application Read Forward 1 1 Application Read Reverse 81 Illumina Genome Analyzer IIx SRX072647 Esac2 Sequence SRP006965 FGMG cDNA from the Clontech SMARTer PCR cDNA Synthesis Kit was used as the basis for the library preparation. (NOTE that the SMARTer protocol is optimized for small tissue samples, and slightly modified from the SMART protocol used for the Seedling and Pollen samples.) Following the illumina Paired-End Sample Prep kit protocol (recounted briefly here), cDNA was first sheared using a nebulizer and then purified. DNA fragment ends were repaired, dA was added to the ends, and adapters for the illumina sequencing protocol were added. The prepared DNA fragments were size-selected via agarose gel electrophoresis at a median of ~320 bp, and purified via the Wizard SV Gel and PCR Cleanup System (Promega). Library Construction Date: 9/30/09 SRS211664 FGMG-ES2 MaizeEmbryoSac2 RNA-Seq TRANSCRIPTOMIC size fractionation 160 0 Application Read Forward 1 1 Application Read Reverse 81 Illumina Genome Analyzer IIx SRX072648 Esac3 Sequence SRP006965 FGMG cDNA from the Clontech SMARTer PCR cDNA Synthesis Kit was used as the basis for the library preparation. (NOTE that the SMARTer protocol is optimized for small tissue samples, and slightly modified from the SMART protocol used for the Seedling and Pollen samples.) Following the illumina Paired-End Sample Prep kit protocol (recounted briefly here), cDNA was first sheared using a nebulizer and then purified. DNA fragment ends were repaired, dA was added to the ends, and adapters for the illumina sequencing protocol were added. The prepared DNA fragments were size-selected via agarose gel electrophoresis at a median of ~320 bp, and purified via the Wizard SV Gel and PCR Cleanup System (Promega). Library Construction Date: 9/30/09 SRS211665 FGMG-ES3 MaizeEmbryoSac3 RNA-Seq TRANSCRIPTOMIC size fractionation 160 0 Application Read Forward 1 1 Application Read Reverse 81 Illumina Genome Analyzer IIx