SRX076733 TEX-treated library SRP007195 454 pyrosequencing of c.elegans noncoding transcriptome RNA in the size ranging from 50 to 500nt were excised from the gel. The RNA was electro-eluted in TBE buffer (0.5X) using the D-Tube™ Dialyzer Maxi MWCO 3.5 kDa, and using the D-Tube with high efficiency (>90%) as the manufacturer's instructions. After polyadenylated RNAs and rRNAs were removed using an adapted MicrobExpress kit, the RNA sample was split into two aliquotes, of which one was treated with Terminator 5'-monophosphate dependent exonuclease (Epicentre).the treated RNA sample was dephosphorylated with FastAP, followed by ligation to the 3'-adaptor oligonucleotide (UUUUGACCACGGTACCCAG; underlined bases are RNA) by T4 RNA ligase. The 3'-end ligated RNAs were reverse transcribed by SMARTer™ PCR cDNA Synthesis kit using an oligo complementary to the 3’-RT adaptor (CTGGGTACCGTGGTCAAA). The two cDNA libraries were amplified by PCR using the Advantage® 2 PCR kit, followed by purification of the PCR products using PureLink™ PCR Purification Kit. The two libraries were then sequenced on a Roche/454 GS-FLX system. SRS212020 C.elegans mixed mixed-stage worms TEX-treated library RNA-Seq TRANSCRIPTOMIC size fractionation 400 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX SRX076734 control-library SRP007195 454 pyrosequencing of c.elegans noncoding transcriptome RNA in the size ranging from 50 to 500nt were excised from the gel. The RNA was electro-eluted in TBE buffer (0.5X) using the D-Tube™ Dialyzer Maxi MWCO 3.5 kDa, and using the D-Tube with high efficiency (>90%) as the manufacturer’s instructions. After polyadenylated RNAs and rRNAs were removed using an adapted MicrobExpress kit (Ambion), the RNA sample was split into two aliquotes, of which one was treated with Terminator 5’-monophosphate dependent exonuclease (Epicentre), and the other left untreated.The untreated RNA sample was dephosphorylated with FastAP, followed by ligation to the 3’-adaptor oligonucleotide (UUUUGACCACGGTACCCAG; underlined bases are RNA) by T4 RNA ligase. The 3’-end ligated RNAs were reverse transcribed by SMARTer™ PCR cDNA Synthesis kit using an oligo complementary to the 3’-RT adaptor (CTGGGTACCGTGGTCAAA). The two cDNA libraries were amplified by PCR using the Advantage® 2 PCR kit, followed by purification of the PCR products using PureLink™ PCR Purification Kit. The two libraries were then sequenced on a Roche/454 GS-FLX system. SRS212020 C.elegans mixed mixed-stage worms control library RNA-Seq TRANSCRIPTOMIC size fractionation 400 0 Technical Read Adapter 1 1 Application Read Forward 5 454 GS FLX